17 resultados para Old world wines
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
The classification and phylogenetic relationships of the Old World monkeys are still controversial. For Asian colobines, from three to nine genera were recognized by different primatologists. In the present study, we have sequenced a 424 bp mitochondrial tRNA(Thr) gene and cytochrome b gene fragment from Macaca mulatta, Mandrillus sphinx, Mandrillus leucophaeus, Semnopithecus entellus, Trachypithecus vetulus, T. johnii, T. phayrei, T. francoisi, Pygathrix nemaeus, Rhinopithecus roxellanae, R. bieti, R. avunculus, Nasalis larvatus, and Colobus polykomos in order to gain independent information on the classification and phylogenetic relationships of those species. Phylogenetic trees were constructed with parsimony analysis by weighting transversions 5 or 10 fold greater than transitions. Our results support the following conclusions: (1) the Old World monkeys are divided into two subfamilies; (2) that among the colobines, Colobus, the African group, diverged first, and Nasalis and Rhinopithecus form a sister clade to Pygathrix; (3) that there are two clades within leaf monkeys, i.e. 1) S. entellus, T. johnii, and T. vetulus, and 2) T, phayrei and T. francoisi; (4) that Rhinopithecus avunculus, R. roxellanae, and R. bieti are closely related to each other, and they should be placed into the same subgenus; (5) that Rhinopithecus is a distinct genus; and (6) that the ancestors of Asian colobines migrated from Africa to Asia during the late Pliocene or early Pleistocene.
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The growth hormone (GH) gene family represents an erratic and complex evolutionary pattern, involving many evolutionary events, such as multiple gene duplications, positive selection, the birth-and-death process and gene conversions. In the present study, we cloned and sequenced GH-like genes from three species of New World monkeys (NWM). Phylogenetic analysis strongly suggest monophyly for NWM GH-like genes with respect to those of Old World monkeys (OWM) and hominoids, indicating that independent gene duplications have occurred in NWM GH-like genes. There are three main clusters of genes in putatively functional NWM GH-like genes, according to our gene tree. Comparison of the ratios of nonsynonymous and synonymous substitutions revealed that these three clusters of genes evolved under different kinds of selective pressures. Detailed analysis of the evolution of pseudogenes showed that the evolutionary pattern of this gene family in platyrrhines is in agreement with the so-called birth-and-death process.
Resumo:
Growth hormone is a classic molecule in the study of the molecular clock hypothesis as it exhibits a relatively constant rate of evolution in most mammalian orders except primates and artiodactyls, where dramatically enhanced rate of evolution (25-50-fold) has been reported. The rapid evolution of primate growth hormone occurred after the divergence of tarsiers and simians, but before the separation of old world monkeys (OWM) from new world monkeys (NWM). Interestingly, this event of rapid sequence evolution coincided with multiple duplications of the growth hormone gene, suggesting gene duplication as a possible cause of the accelerated sequence evolution. Here we determined 21 different GH-like sequences from four species of OWM and hominoids. Combining with published sequences from OWM and hominoids, our analysis demonstrates that multiple gene duplications and several gene conversion events both occurred in the evolutionary history of this gene family in OWM/hominoids. The episode of recent duplications of CSH-like genes in gibbon is accompanied with rapid sequence evolution likely resulting from relaxation of purifying selection. GHN genes in both hominoids and OWM are under strong purifying selection. In contrast, CSH genes in both lineages are probably not. GHV genes in OWM and hominoids evolved at different evolutionary rates and underwent different selective constraints. Our results disclosed the complex history of the primate growth hormone gene family and raised intriguing questions on the consequences of these evolutionary events. © 2005 Elsevier B.V. All rights reserved.
Resumo:
Chromosomal homologies were established between human and two Chinese langurs (Semnopithecus francoisi, 2n=44, and S. phayrei, 2n=44) by chromosome painting with chromosome-specific DNA probes of all human chromosomes except the Y. Both langur species showed identical hybridization patterns in addition to similar G-banding patterns. In total, 23 human chromosome-specific probes detected 30 homologous chromosome segments in a haploid langur genome. Except for human chromosomes 1, 2, 6, 16 and 19 probes, which each gave signals on two non-homologous langur chromosomes respectively, all other probes each hybridized to a single chromosome. The results indicate a high degree of conservation of chromosomal synteny between human and these two Chinese langurs. The human chromosome 2 probe painted the entire euchromatic regions of langur chromosomes 14 and 19. Human chromosome 1 probe hybridized to three regions on langur autosomes, one region on langur chromosome 4 and two regions on langur chromosome 5. Human 19 probe hybridized on the same pattern to one region on chromosome 4 and to two regions on langur chromosome 5, where it alternated with the human chromosome 1 probe. Human 6 and 16 probes both hybridized to one region on each of the two langur autosomes 15 and 18. Only two langur chromosomes (12 and 21) were each labelled by probes specific for two whole human chromosomes (14 and 15 and 21 and 22 respectively). Comparison of the hybridization patterns of human painting probes on these two langurs with the data on other Old World primates suggests that reciprocal and Robertsonian translocations as will as inversions could have occurred since the divergance of human and the langurs from a common ancestor. This comparison also indicates that Asian colobines are karyotypically more closely related to each other that to African colobines.
Resumo:
Neuropsin is a secreted-type serine protease involved in learning and memory. The type II splice form of neuropsin is abundantly expressed in the human brain but not in the mouse brain. We sequenced the type II-spliced region of neuropsin gene in humans and representative nonhuman primate species. Our comparative sequence analysis showed that only the hominoid species (humans and apes) have the intact open reading frame of the type II splice form, indicating that the type II neuropsin originated recently in the primate lineage about 18 MYA. Expression analysis using RT-PCR detected abundant expression of the type II form in the frontal lobe of the adult human brain, but no expression was detected in the brains of lesser apes and Old World monkeys, indicating that the type II form of neuropsin only became functional in recent time, and it might contribute to the progressive change of cognitive abilities during primate evolution.
Resumo:
Neurotrypsin is one of the extra-cellular serine proteases that are predominantly expressed in the brain and involved in neuronal development and function. Mutations in humans are associated with autosomal recessive non-syndromic mental retardation (MR). We studied the molecular evolution of neurotrypsin by sequencing the coding region of neurotrypsin in 11 representative non-human primate species covering great apes, lesser apes, Old World monkeys and New World monkeys. Our results demonstrated a strong functional constraint of neurotrypsin that was caused by strong purifying selection during primate evolution, an implication of an essential functional role of neurotrypsin in primate cognition. Further analysis indicated that the purifying selection was in fact acting on the SRCR domains of neurotrypsin, which mediate the binding activity of neurotrypsin to cell surface or extracellular proteins. In addition, by comparing primates with three other mammalian orders, we demonstrated that the absence of the first copy of the SRCR domain (exon 2 and 3) in mouse and rat was due to the deletion of this segment in the murine lineage. Copyright (C) 2005 S. Karger AG, Basel.
Resumo:
Objective: In Old World monkeys, the tripartite motif Sec (TRIM5 alpha) protein confers resistance to HIV-1 infection following virus entry into host cells. However, the pig-tailed macaque (Macaca nemestrina) is an exception and is susceptible to HIV-1 in
Resumo:
We constructed a high redundancy bacterial artificial chromosome library of a seriously endangered Old World Monkey, the Yunnan snub-nosed monkey (Rhinopithecus bieti) from China. This library contains a total of 136 320 BAC clones. The average insert size of BAC clones was estimated to be 148 kb. The percentage of small inserts (50-100 kb) is 2.74%, and only 2.67% non-recombinant clones were observed. Assuming a similar genome size with closely related primate species, the Yunnan snub-nosed monkey BAC library has at least six times the genome coverage. By end sequencing of randomly selected BAC clones, we generated 201 sequence tags for the library. A total of 139 end-sequenced BAC clones were mapped onto the chromosomes of Yunnan snub-nosed monkey by fluorescence in-situ hybridization, demonstrating a high degree of synteny conservation between humans and Yunnan snub-nosed monkeys. Blast search against human genome showed a good correlation between the number of hit clones and the size of the chromosomes, an indication of unbiased chromosomal distribution of the BAC library. This library and the mapped BAC clones will serve as a valuable resource in comparative genomics studies and large-scale genome sequencing of nonhuman primates. The DNA sequence data reported in this paper were deposited in GenBank and assigned the accession number CG891489-CG891703.
Resumo:
测定人猿超科(人、黑猩猩、大猩猩、红毛猩猩和长臂猿)和旧大陆猴(猕猴和叶猴)7种高等灵长类FKN全基因序列,探讨其系统进化分析.用简并引物PCR(Degenerated PCR)法分别扩增FKN的3个外显子,其产物经琼脂糖凝胶回收、纯化后测序,然后用BioEdit软件剪切拼接FKN基因全序列,用DNAStar比对后比较基因和氨基酸序列同源性,Mega软件重构FKN基因进化树,应用Datamonkey分析FKN的负选择位点.序列分析发现人猿超科较旧大陆猴FKN基因除了有散在的点突变外,还有一明显的30 bp的核苷酸缺失突变;人FKN基因序列与黑猩猩、大猩猩、红毛猩猩、长臂猿、猕猴和叶猴的同源性分别是99.2%、98.4%、98.1%、96.5%%、95.95和93.8%,由此推导的氨基酸序列同源性分别是98.5%、98.0%、97.7%、94.7%、93.7%和90.5%;FKN基因进化树表明人与黑猩猩关系更近,FKN基因进化和通常认为的物种进化一致;Datamonkey分析结果显示FKN存在3个负选择位点53Q.84D、239N.成功获得人、黑猩猩、大猩猩、红毛猩猩、长臂猿、猕猴和叶猴7种高等灵长类物种FKN全基因序列,为后续探讨FKN在高等灵长类物种进化过程中免疫学功能演变及其结构与功能的关系奠定基础. 作 者: 洪晓武 张亚平 储以微 高海峰 蒋正刚 熊思东 HONG Xiao-Wu ZHANG Ya-Ping CHU Yi-Wei GAO Hai-Feng JIANG Zheng-Gang XIONG Si-Dong 作者单位: 洪晓武,储以微,高海峰,蒋正刚,熊思东,HONG Xiao-Wu,CHU Yi-Wei,GAO Hai-Feng,JIANG Zheng-Gang,XIONG Si-Dong(复旦大学上海医学院免疫学系,免疫生物学研究所,上海,200032) 张亚平,ZHANG Ya-Ping(中国科学院昆明动物研究所细胞与分子进化开放实验室,昆明,650223) 刊 名: 遗传 ISTIC PKU 英文刊名: HEREDITAS 年,卷(期): 2008 30(5) 分类号: Q94 关键词: 人猿超科 旧大陆猴 FKN 测序 进化分析 机标分类号: R5 S86 机标关键词: 大陆灵长类全基因序列测定系统进化分析phylogenetic analysis基因进化树黑猩猩序列同源性长臂猿物种进化猕猴红毛负选择氨基酸琼脂糖凝胶基因全序列序列分析位点软件重构 基金项目: 国家自然科学基金,复旦大学校科研和教改项目 DOI:
Resumo:
Evidence of incongruence between mitochondrial and nuclear gene trees is now becoming documented with increasing frequency. Among the Old World monkeys, this discordance has been well demonstrated in the Cercopithecinae, but has not yet been investigated
Resumo:
Rhodopsin, encoded by the gene Rhodopsin (RH1), is extremely sensitive to light, and is responsible for dim-light vision. Bats are nocturnal mammals that inhabit poor light environments. Megabats (Old-World fruit bats) generally have well-developed eyes, while microbats (insectivorous bats) have developed echolocation and in general their eyes were degraded, however, dramatic differences in the eyes, and their reliance on vision, exist in this group. In this study, we examined the rod opsin gene (RH1), and compared its evolution to that of two cone opsin genes (SWS1 and M/LWS). While phylogenetic reconstruction with the cone opsin genes SWS1 and M/LWS generated a species tree in accord with expectations, the RH1 gene tree united Pteropodidae (Old-World fruit bats) and Yangochiroptera, with very high bootstrap values, suggesting the possibility of convergent evolution. The hypothesis of convergent evolution was further supported when nonsynonymous sites or amino acid sequences were used to construct phylogenies. Reconstructed RH1 sequences at internal nodes of the bat species phylogeny showed that: (1) Old-World fruit bats share an amino acid change (S270G) with the tomb bat; (2) Miniopterus share two amino acid changes (V104I, M183L) with Rhinolophoidea; (3) the amino acid replacement I123V occurred independently on four branches, and the replacements L99M, L266V and I286V occurred each on two branches. The multiple parallel amino acid replacements that occurred in the evolution of bat RH1 suggest the possibility of multiple convergences of their ecological specialization (i.e., various photic environments) during adaptation for the nocturnal lifestyle, and suggest that further attention is needed on the study of the ecology and behavior of bats.
Resumo:
Background: The pig-tailed macaques are the only Old World monkeys known to be susceptible to human immunodeficiency virus type 1 (HIV-1) infection. We have previously reported that the TRIM5-Cyclophilin A (TRIMCyp) fusion in pig-tailed macaques (Macaca n
Resumo:
In order to understand how mandibular structure differs among the Chinese cercopithecoids (Rhinopithecus, Trachypithecus and Macaca), particularly the uniqueness of the snub-nosed monkeys (Rhinopithecus), we analysed ten mandibular measurements by principal components analysis (PCA), and examined scaling patterns. The results provided by the PCA illustrated differences due to size among the cercopithecoids and the relationship between colobines (Trachypithecus and Rhinopithecus) and cercopithecines, in which macaques (Macaca) are included. Allometric analysis indicated that, biomechanically, there is not a marked difference between macaques and leaf-eating monkeys. This may be associated with the fact that both share some similar ecology and niches in south and southwest China. The snub-nosed monkeys exhibit a significantly more robust mandible, evident in the symphysis, corpus, condyle, and masticatory momentum arm. This supports the hypothesis, based on the study of dental structure, that Rhinopithecus is a unique group in Asian Old World monkeys (OWMs) and has developed some unique characteristics in order to adapt to the tough food available in the severe cold climate of the Plateaux of Qinghai-Tibet, Yun-Gui and Qingling in China.
Resumo:
天然免疫分子TRIM5α(tripartite motif protein 5α)是近年来发现的一种重要的宿主细胞内逆转录病毒限制因子。在灵长类动物细胞中,TRIM5α蛋白可以在病毒进入细胞后、逆转录前的阶段抑制HIV-1、N-MLV和EIAV等逆转录病毒的复制。由于TRIM5α分子的作用,绝大部分旧大陆猴(Old World monkey)都不能感染HIV-1。而在新大陆猴(New World monkey)中,鹰猴是唯一不感染HIV-1的灵长类动物。研究证明,鹰猴细胞中存在的TRIM5-CypA融合蛋白(owl monkey TRIM5-CypA,omTRIMCyp)介导了抗HIV-1的作用,从而使鹰猴不能感染HIV-1。研究证明,平顶猴是旧大陆猴中唯一报道可以感染HIV-1的灵长类动物,但是其感染HIV-1的机制并不清楚。根据现行的灵长类动物分类学,原属平顶猴群体(M. nemestrina group)的三个亚种分为猕猴属的三个不同种:巽他平顶猴(Sunda pig-tailed macaque,M. nemestrina),北平顶猴(Northern pig-tailed macaque,M. leonina)和明打威猴(Mentawai macaque,M. pagensis)。本论文对中国云南境内北平顶猴TRIM5基因座和感染HIV-1的相关性进行了研究。通过PCR和测序对北平顶猴基因组TRIM5基因座进行分析,发现一个CypA假基因的cDNA通过逆转座机制插入至TRIM5基因座的3’-UTR区域,形成了一个不同于鹰猴TRIM5-CypA的新型融合基因npmTRIMCyp(northern pig-tailed macaque TRIM5-CypA)。通过RT-PCR对npmTRIMCyp融合基因的转录本进行分析,我们鉴定出npmTRIMCyp共有3种不同的选择性剪接产物,分别为npmTRIMCypV1-V3。进一步克隆和测序这3种不同选择性剪接体,通过丰度和序列分析证实:npmTRIMCypV2是优势剪接体,可能在该融合基因产物的功能中发挥作用。研究发现北平顶猴npmTRIMCyp融合基因主要转录本中外显子7和8均被剪切掉。外显子7剪接丢失机制源于TRIM5第6内含子内 3’剪接位点的G/T突变。我们克隆了npmTRIMCyp融合基因cDNA的蛋白编码区ORF,并构建了重组表达npmTRIMCyp的载体,转染HeLa和HeLa-T4细胞并获得稳定表达的细胞株。通过感染HIV-1证实,npmTRIMCyp融合蛋白不能够限制HIV-1的感染和复制,这可能是北平顶猴作为旧大陆猴中唯一对HIV-1易感的灵长类动物的重要分子机制之一。通过HIV-1感染灵长类动物PBMCs实验证实,北平顶猴可以感染HIV-1。npmTRIMCyp可以有效地限制HIV-2ROD的复制,但对SIVmac239只有十分微弱的限制活性。通过构建鹰猴omTRIMCyp和北平顶猴npmTRIMCyp的置换剪接体(SWAP-1和SWAP-2),转染融合基因及其置换剪接体的CRFK细胞激光共聚焦实验证明,npmTRIMCyp、SWAP1和SWAP2在细胞内主要存在于胞浆中。稳定表达融合蛋白和置换剪接体的CRFK细胞感染HIV-1-GFP-VSVG分析表明,含omTRIMCyp外显子7的SWAP-1和SWAP-2均具有限制HIV-1活性,但SWAP-1的活性更强一些,这表明TRIM5结构域的外显子7可能在介导对HIV-1的限制活性中发挥了协同辅助作用。免疫共沉淀研究表明,npmTRIMCyp不能识别和结合HIV-1的衣壳蛋白。对北平顶猴中介导识别逆转录病毒区域的基因组部分进行了测序,共鉴定出46个多态性位点,表明在北平顶猴识别逆转录病毒衣壳区域存在较高的多态性。
Resumo:
采用样带调查与TWINSPAN分类等方法,对陕北丘陵沟壑区延安、安塞和吴旗174个撂荒地样方的物种组成、出现频率与盖度、及群落类型进行了统计与分类。植被组成结构的统计结果表明:该区自然恢复的植被几乎一半是由禾本科、菊科、豆科和蔷薇科的物种组成,北温带、旧世界温带、世界与泛热带分布成分占到总物种数近75%,且以中旱生、中生和旱生的草本类植物为主,具有典型的温带地面芽植物气候特征。植被的数量分类表明:调查样方基本包括了该区自然恢复的主要植被类型,延安、安塞和吴旗的植被在1年生草本群落到多年生蒿禾类草本群落阶段,依次均以猪毛蒿(Artemisia scoparia)、赖草(Leymussecalinus)、长芒草(Stipa bungeana)、达乌里胡枝子(Lespedeza davurica)、铁杆蒿(Artemisia gmelinii)、茭蒿(Artemisia giraldii)、白羊草(Bothriochloa ischaemun)等为主要优势物种构成的不同组合的植物群落,且这些物种具有较高的盖度和频度;但在植被演替后期,不同植被带及阴阳坡的演替方向却发生了明显的变化。以延安为代表的森林带,阴坡可形成黄...