Sources and transport of dissolved and particulate organic carbon in the Mississippi River estuary and adjacent coastal waters of the northern Gulf of Mexico

Dissolved organic carbon (DOC), stable carbon isotopic (delta(13)C) compositions of DOC and particulate organic carbon (POC), and elemental C/N ratios of POC were measured for samples collected from the lower Mississippi and Atchafalaya rivers and adjacent coastal waters in the northern Gulf of Mexico during the low flow season in June 2000 and high flow season in April 2001. These isotopic and C/N results combined with DOC measurements were used to assess the sources and transport of terrestrial organic matter from the Mississippi and Atchafalaya rivers to the coastal region in the northern Gulf of Mexico. delta(13)C values of both POC (-23.8parts per thousand to -26.8parts per thousand) and DOC (-25.0parts per thousand to -29.0parts per thousand) carried by the two rivers were more depleted than the values measured for the samples collected in the offshore waters. Strong seasonal variations in delta(13)C distributions were observed for both POC and DOC in the surface waters of the region. Fresh water discharge and horizontal mixing played important roles in the distribution and transport of terrestrial POC and DOC offshore. Our results indicate that both POC and DOC exhibited non-conservative behavior during the mixing especially in the mid-salinity range. Based on a simple two end-member mixing model, the comparison of the measured DOC-delta(13)C with the calculated conservative isotopic mixing curve indicated that there was a significant in situ production of marine-derived DOC in the mid- to high-salinity waters consistent with our in situ chlorophyll-a measurements. Our DOC-delta(13)C data suggest that a removal of terrestrial DOC mainly occurred in the high-salinity (>25) waters during the mixing. Our study indicates that the mid- to high- (10-30) salinity range was the most dynamic zone for organic carbon transport and cycling in the Mississippi River estuary. Variability in isotopic and elemental compositions along with variability in DOC and POC concentrations suggest that autochthonous production, bacterial utilization, and photo-oxidation could all play important roles in regulating and removing terrestrial DOC in the northern Gulf of Mexico and further study of these individual processes is warranted. (C) 2004 Elsevier B.V. All rights reserved.

A small trypsin inhibitor from the frog of Odorrana grahami

A novel peptide inhibitor (OGTI) of serine protease with a molecular weight of 1949.8, was purified from the skin secretion of the frog, Odorrana grahami. Of the tested serine proteases, OGTI only inhibited the hydrolysis activity of trypsin on synthetic chromogenic substrate. This precursor deduced from the cDNA sequence is composed of 70 amino acid residues. The mature OGTI contains 17 amino acid residues including a six-residue loop disulfided by two half-cysteines (AVNIPFKVHFRCKAAFC). In addition to its unique six-residue loop, the overall structure and precursor of OGTI are different from those of other serine protease inhibitors. It is also one of the smallest serine protease inhibitors ever found. (C) 2008 Elsevier Masson SAS. All rights reserved.

Cross-shelf circulation in the Yellow and East China Seas indicated by MODIS satellite observations

Ocean color and sea surface temperature data from Moderate Resolution Imaging Spectroradiometer (MODIS) onboard the Terra satellite are used to study the cross-shelf circulation and transport of suspended sediments in the Yellow and the East China Seas. The ocean color images show a significant turbid water plume extending in the southeast direction from the Subei coasts of China to the shelf edge south of Cheju during fall-winter, suggesting significant cross-shelf currents in the Yellow Sea/East China Sea in winter. The currents transport suspended sediments from the area of the old Huanghe mouth into the Okinawa Trough. Part of the turbid plume joins the Yellow Sea Warm Current to enter the Yellow Sea trough in winter. The satellite images suggest that the time scales of cross-shelf transport and surface-to-subsurface descending of the suspended sediments are a few weeks. The turbid plume grows in fall, reaches its maximum expansion and intensity in winter-spring, and subsides in late spring. In summer, the plume becomes coastally trapped. Substantial interannual variations of the intensity and coverage of the turbid plume are indicated by the observations. In comparison, the Changjiang Diluted Water in summer only transports a small amount of the Changjiang suspended sediment to the outer shelf south of Cheju, which does not enter the Yellow Sea owing to the weak intrusion of the Yellow Sea Warm Current in summer. The dynamics of the cross-shelf circulation in the Yellow Sea in winter are hypothesized to be associated with (1) the convergence of the Yellow Sea Coastal Current and the Taiwan Warm Current off the Changjiang mouth and (2) the time-dependent forcing of the northerly wind bursts that drives the intrusion of the Yellow Sea Warm Current. (C) 2007 Elsevier B.V. All rights reserved.