Intraperitoneal injection of extracted microcystins results in hypovolemia and hypotension in crucian carp (Carassius auratus)

Circulatory responses of crucian carp injected intraperitoneally with extracted micro-cystins (MCs) were studied at sublethal and lethal doses (150 and 600 mu g MC kg(-1) body mass, respectively). Mean arterial blood pressure (MAP), heart rate, hematocrit (Hct), red blood cell (RBC) counts, and circulating blood volume (BV) were assayed at 0, 1, 3, 12, 24, and 48 h post-toxin administration. MAP decreased significantly in a dose-dependent manner over time. Within the 48-h test period, the lethal dose as well as the sublethal dose resulted in a steady decline of MAP without recovery. Heart rate significantly increased within 24 h post-injection as blood pressure significantly dropped, then showed a terminal decline to the control level. The dose-dependent decreases in BV and Hct were directly related to the drop in MAP. Intraperitoneal injection of a lethal dose of MCs led to hepatic and gill hemorrhage. Consequently, crucian carp given MCs suffered from hypovolemic hypotensive shock. (C) 2009 Elsevier Ltd. All rights reserved.

Direct Numerical Simulation of Shock/Turbulent Boundary Layer Interaction in a Supersonic Compression Ramp

A direct numerical simulation of the shock/turbulent boundary layer interaction flow in a supersonic 24-degree compression ramp is conducted with the free stream Mach number 2.9. The blow-and-suction disturbance in the upstream wall boundary is used to trigger the transition. Both the mean wall pressure and the velocity profiles agree with those of the experimental data, which validates the simulation. The turbulent kinetic energy budget in the separation region is analyzed. Results show that the turbulent production term increases fast in the separation region, while the turbulent dissipation term reaches its peak in the near-wall region. The turbulent transport term contributes to the balance of the turbulent conduction and turbulent dissipation. Based on the analysis of instantaneous pressure in the downstream region of the mean shock and that in the separation bubble, the authors suggest that the low frequency oscillation of the shock is not caused by the upstream turbulent disturbance, but rather the instability of separation bubble.