Developing A Microfluidic-Based System To Quantify Cell Capture Efficiency

Micro-fabrication technology has substantial potential for identifying molecular markers expressed on the surfaces of tissue cells and viruses. It has been found in several conceptual prototypes that cells with such markers are able to be captured by their antibodies immobilized on microchannel substrates and unbound cells are flushed out by a driven flow. The feasibility and reliability of such a microfluidic-based assay, however, remains to be further tested. In the current work, we developed a microfluidic-based system consisting of a microfluidic chip, an image grabbing unit, data acquisition and analysis software, as well as a supporting base. Specific binding of CD59-expressed or BSA-coupled human red blood cells (RBCs) to anti-CD59 or anti-BSA antibody-immobilized chip surfaces was quantified by capture efficiency and by the fraction of bound cells. Impacts of respective flow rate, cell concentration, antibody concentration and site density were tested systematically. The measured data indicated that the assay was robust. The robustness was further confirmed by capture efficiencies measured from an independent ELISA-based cell binding assay. These results demonstrated that the system developed provided a new platform to effectively quantify cellular surface markers effectively, which promoted the potential applications in both biological studies and clinical diagnoses.

Laser drilling improving circuit board manufacturing

We reported here a novel technique for laser high speed drillings on Printed Circuit Boards (PCBs). A CNC solid laser based system is developed to drill through and blind vias as an alternative to mechanical drilling. The system employs an Acousto-Optic Q-switched Nd: YAG laser, a computer control system and an X-Y moving table which can handle up to 400 x 400 mm PCB. With a special designed cavity the laser system works in a pulsed operation in order to generate pulses with width down to 0.5 mu s and maximum peak power over 10kW at 10k repetition rate. Delivered by an improved optical beam transforming system, the focused laser beam can drill hobs including blind vias on PCBs with diameter in the range of 0.1 - 0.4 mm and at up to 300 - 500 vias per second (depending on the construction of PCBs). By means of a CNC X-Y moving system, laser pulses with pulse-to-pulse superior repeatability can be fired at desired location on a PCBs with high accuracy. This alternative technology for drilling through or blind vias on PCBs or PWBs (printed wiring boards) will obviously enhance the capability to printed boards manufacturing.

Structure and electrical properties of Pr ans Sm doped CeO2 and Ce2MoO15 based solid electrolytes

Sin and Pr doped CeO2 and Ce6MoO15 based materials were synthesized by sol-gel method. The structure of the powders were characterized by X-ray diffraction (XRD), Raman spectra, field emission scanning electron microscopy(FE-SEM) and the electrical conductivity of the samples was investigated by AC impedance spectroscopy. By comparing the structure and electrical properties of different systems, it could be concluded that the electrical property of Ce6MoO15 based system is better than that of CeO2 system. The added Mo element resulted in the increase of gain size and improved the grain boundary conductivity notably below 600 degrees C, while the Pr dopant induced the smaller grain size and improved the grain boundary conductivity of the materials.

一种基于神经网络的视觉定位系统

本文提出了一种基于人工神经网络的多线阵相机系统标定与 3D定位方法 ,并应用于基于多线阵相机构成的视觉空间定位系统 .该视觉定位系统利用了线阵相机的快速性与高分辨率的特点 ,非平行空间投影面相交定位的基本原理 ,实现了这种结构下快速、高精度空间定位 .实验表明 ,人工神经网络的定位方法简化了多线阵视觉定位系统标定与定位计算的复杂性 ,在定位精度上达到了良好效果 .为机器人位置反馈控制提供了有效的技术途径

基于立体视觉的移动机器人自主导航定位系统

描述了一种基于立体视觉的移动机器人自主导航定位系统。该系统采用双目立体视觉完成环境特征的 3D信息提取 ,实时计算出机器人相对作业目标的位姿 (6D)关系 ,导引移动机器人控制系统按目标导向进行运动。系统在相对位姿计算中采用旋动(Screw)理论 ,将带约束的多变量函数的非线性优化问题转化为线性方程组的最小二乘问题 ,简化了计算复杂性。实验表明 ,这个导航定位系统在定位精度和数据处理速度上均可满足机器人导航的要求。

一种基于视觉的移动机器人定位系统

具有自主的全局定位能力是自主式移动机器人传感器系统的一项重要功能 .为了实现这个目的 ,国内外均在不断地研究发展各种定位传感器系统 .这里介绍了一种采用光学原理的全方位位置传感器系统 .该传感器系统由主动式路标、视觉传感器、图象采集与数据处理系统组成 .其视觉传感器和数据处理系统可安装在移动机器人上 ,然后可通过观测路标和视角定位的方法 ,计算出机器人在世界坐标系中的位置和方向 .实验证明 ,该系统可以实现机器人的在线定位 ,其采样速率和精度能够满足实用要求 .

A shortest path algorithm for moving objects in spatial network databases

One of the most important kinds of queries in Spatial Network Databases (SNDB) to support location-based services (LBS) is the shortest path query. Given an object in a network, e.g. a location of a car on a road network, and a set of objects of interests, e.g. hotels,gas station, and car, the shortest path query returns the shortest path from the query object to interested objects. The studies of shortest path query have two kinds of ways, online processing and preprocessing. The studies of preprocessing suppose that the interest objects are static. This paper proposes a shortest path algorithm with a set of index structures to support the situation of moving objects. This algorithm can transform a dynamic problem to a static problem. In this paper we focus on road networks. However, our algorithms do not use any domain specific information, and therefore can be applied to any network. This algorithm’s complexity is O(klog2 i), and traditional Dijkstra’s complexity is O((i + k)2).

基于草图界面的应用系统框架研究与实现

现有的草图系统重在设计对象外形的表达，忽略了隐含在设计过程中用户思维活动的信息；另一方面，目前笔的交互方式尚不能自然、流畅地表达用户设计意图．通过分析草图界面信息对象和交互过程的特点，提出面向构思设计的应用系统框架ISF，以满足设计意图原型的快速生成；同时结合交互的任务、环境和用户偏好等上下文信息，建立从物理设备到逻辑工具映射的交互模型，旨在为此类系统的交互设计提供一定指导的方法．最后给出面向轻纺行业服装设计的应用实例——草图图板系统．应用结果表明，ISF能够为此类草图应用系统的设计给予一定的指导，满足自然、流畅的交互设计要求．

Review on study of resonance electron capture mass spectrometry

Resonance electron capture mass spectrometry, in which an additional information coordinate, the energy of electron capture, is applied, has a high sensitivity and a high specificity. It is extensively used to study the structure elucidation, the mechanism of ion formation and the detection, identification and quantification of organic substances in mixture.

基于非线性修正的多线阵像机的空间定位

介绍了一种基于多线阵像机构成的视觉空间定位系统.该系统利用线阵像机的快速性与高分辨率的特点,采用了非平行空间投影面相交定位的基本原理,利用几何投影关系定位求解的方法,实现了多线阵像机视觉系统的空间定位.并提出了多线阵像机的神经网络非线性修正方法,使修正后的PSD能在较宽的位置范围内输出高线性度的信号.实验结果表明,基于非线性修正的多线阵像机位姿测量系统简化了立体视觉空间定位计算的复杂性,在定位精度、定位范围和采样速度上均达到了良好效果.

智能制造装备数字化设计与验证系统研究

智能制造装备的设计水平不高是制约我国装备制造业发展因素之一。在阐述智能制造装备特点的基础上,提出了一种基于CORBA规范的智能制造装备数字化设计与验证系统的体系结构,并探讨了实现该系统的关键技术。该系统的目的是降低智能制造设备开发成本,缩短开发周期,提高智能制造装备的设计质量。

路标定位中的优化选择策略

基于合作路标的三角定位是自主式移动机器人的主要定位方法。路标的优化选择是提高定位算法精度的关键。本文在给出该算法原理的基础上 ,通过理论分析和实验证明了路标优化选择对提高定位算法精度的作用 ,并介绍了路标优化选择的准则

基于意象知识的消歧体系

本文提出了一种可以表示常识及语言知识的意象知识体系。在这种知识的形式化表示基础上,给出了NLP中的消歧知识及其表示形式,以及基于消歧知识的消歧策略。最后,论述了这种方法实现上的可行性。

胜利勘探数据中心及勘探辅助决策系统研究

In the paper through extensive study and design, the technical plan for establishing the exploration database center is made to combine imported and self developed techniques. By research and repeated experiment a modern database center has been set up with its hardware and network having advanced performance, its system well configured, its data store and management complete, and its data support being fast and direct. Through study on the theory, method and model of decision an exploration decision assistant schema is designed with one decision plan of well location decision support system being evaluated and put into action. 1. Study on the establishment of Shengli exploration database center Research is made on the hardware configuration of the database center including its workstations and all connected hardware and system. The hardware of the database center is formed by connecting workstations, microcomputer workstations, disk arrays, and those equipments used for seismic processing and interpretation. Research on the data store and management includes the analysis of the contents to be managed, data flow, data standard, data QC, data backup and restore policy, optimization of database system. A reasonable data management regulation and workflow is made and the scientific exploration data management system is created. Data load is done by working out a schedule firstly and at last 200 more projects of seismic surveys has been loaded amount to 25TB. 2. Exploration work support system and its application Seismic data processing system support has the following features, automatic extraction of seismic attributes, GIS navigation, data order, extraction of any sized data cube, pseudo huge capacity disk array, standard output exchange format etc. The prestack data can be accessed by the processing system or data can be transferred to other processing system through standard exchange format. For supporting seismic interpretation system the following features exist such as auto scan and store of interpretation result, internal data quality control etc. the interpretation system is connected directly with database center to get real time support of seismic data, formation data and well data. Comprehensive geological study support is done through intranet with the ability to query or display data graphically on the navigation system under some geological constraints. Production management support system is mainly used to collect, analyze and display production data with its core technology on the controlled data collection and creation of multiple standard forms. 3. exploration decision support system design By classification of workflow and data flow of all the exploration stages and study on decision theory and method, target of each decision step, decision model and requirement, three concept models has been formed for the Shengli exploration decision support system including the exploration distribution support system, the well location support system and production management support system. the well location decision support system has passed evaluation and been put into action. 4. Technical advance Hardware and software match with high performance for the database center. By combining parallel computer system, database server, huge capacity ATL, disk array, network and firewall together to create the first exploration database center in China with reasonable configuration, high performance and able to manage the whole data sets of exploration. Huge exploration data management technology is formed where exploration data standards and management regulations are made to guarantee data quality, safety and security. Multifunction query and support system for comprehensive exploration information support. It includes support system for geological study, seismic processing and interpretation and production management. In the system a lot of new database and computer technology have been used to provide real time information support for exploration work. Finally is the design of Shengli exploration decision support system. 5. Application and benefit Data storage has reached the amount of 25TB with thousand of users in Shengli oil field to access data to improve work efficiency multiple times. The technology has also been applied by many other units of SINOPEC. Its application of providing data to a project named Exploration achievements and Evaluation of Favorable Targets in Hekou Area shortened the data preparation period from 30 days to 2 days, enriching data abundance 15 percent and getting information support from the database center perfectly. Its application to provide former processed result for a project named Pre-stack depth migration in Guxi fracture zone reduced the amount of repeated process and shortened work period of one month and improved processing precision and quality, saving capital investment of data processing of 30 million yuan. It application by providing project database automatically in project named Geological and seismic study of southern slope zone of Dongying Sag shortened data preparation time so that researchers have more time to do research, thus to improve interpretation precision and quality.

注意在视觉刺激位置编码中的作用

There is extensive agreement that attention may play a role in spatial stimmlus coding (Lu & Proctor, 1995). Some authors investigated the effects of spatial attention on the spatial coding by using spatial cueing procedure and spatial Stroop task. The finding was that the stroop effects were modulated by spatial cueing. Three hypotheses including attentional shift account, referential coding account, and event integration account were used to explain the modulation of spatial cueing over the spatial Stroop effects. In these previous studies, on validly cued trials, cue and target not only appeared at the same location, but also in the same object, which resulted in both location and object cued. Consequently, the modulation of spatial attentional cueing over spatial Stroop effects was confounded with the role of object-based attention. In the third chapter of this dissertation, using a modification of double rectangles cueing procedure developed by Egly, Driver and Rafal (1994) and spatial Stroop task employed by Lupiáñez and Funes (2005), separate effects of spatial attention and object-based attention on the location code of visual stimuli were investigated. Across four experiments, the combined results showed that spatial Stroop effects were modulate by object-based attention, but not by location-based attention. This pattern of results could be well explained by event integration account, but not by attentional shift account and referential coding account. In the fourth chapter, on the basis of the prior chapter, whether the modulation of attentional cueing on location code occurred at the stage of perceptual identification or response choice was investigated. The findings were that object-based attention modulated spatial Stroop effects and did not modulate the Simon effects, whereas spatial attention did not modulate Stroop and Simon effects. This pattern of results partially replicated the outcome of the previous chapter. The previous studies generally argued that the conflicts of spatial Stroop task and Simon task respectively occurred at at the stage of perceptual identification and response choice. Therefore, it is likely to conclude that the modulation of attention over spatial Stroop effect was mediated by object-based attention, and this modulation occurred at the stage perceptual identification. Considering that the previous studies mostly investigated the effects of attention captured by abrupt onset on the spatial Stroop effects, few studies investigated the effects of attention captured by offset cue on the spatial Stroop effects. The aim of the fifth chapter was to investigate the role of attention induced by offset and abrupt onset cue in the spatial Stroop task. These results showed that attention elicited by offset cue or abrupt onset cue modulated the spatial Stroop effects, which reconciled with event integration account.