The sequence and de novo assembly of the giant panda genome

Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.

对PFU法中MacArthur-Wilson平衡模型的修改

考虑到环境压迫的因素,对PFU法中的MacArthur-Wilson平衡模型作了修改,推导出一个更为适用的新模型。小野外生态试验和室内毒理试验中提出4个功能参数(S_(eq),G,H,T_(90％))用于环境的生物监测。使用Monte Garlo序贯随机模拟算法和Marquardt算法估计模型参数并完成计算机程序的编制,最后还使用拟合差异度法检验实验模型。