银鲫肌酸激酶M3-CK cDNA的克隆及其表达特征

用抑制性差减杂交结合SMARTcDNA合成和RACE PCR技术克隆到雌核发育银鲫 (Carassiusauratusgibelio)肌酸激酶M 3 CK基因的全长cDNA。银鲫M 3 CKcDNA全长 15 5 1bp ,编码 380个氨基酸 ,与普通鲤鱼(Cyprinuscarpio)M3 CK的氨基酸序列同源性高达 95 %。种系分析表明 ,银鲫M3 CK与其它脊椎动物的肌肉型肌酸激酶聚为较近的一支 ,与鲤鱼的M 3 CK聚在一起 ,与脑特异型肌酸激酶及线粒体型肌酸激酶分歧较大。虚拟Northern

Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents.

Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents.

Non-die explosive forming of spherical pressure-vessels

This paper presents a newly developed method of manufacturing spherical pressure vessels based on the technology of non-die explosive forming. Compared with the traditional method, this technology does not need any dies and pressing equipment, so that the cost of the production process can be greatly reduced, especially for vessels of less than 100 m3 capacity.

Magnetic properties of sintered sm2fe17ny magnets

Sintered magnets of Sm2Fe17Ny nitrides, with a density of 6.0-7.4 g/cm3, have been prepared by using an explosion technique. Both crystalline structure and the magnetic properties of Sm2Fe17Ny nitrides were retained in the process. The sintered magnet had a remanence B(r)=0.83 T, an intrinsic coercivity mu(0i)H(c)=0.57 T and an energy product (BH)max=88 kJ/m3. The temperature dependence of coercivity and remanence were also measured. The temperature coefficients alpha of remanence and beta of coercivity are -0.076%/degrees-C and -0.51%/degrees-C, respectively.

空气分级燃烧器稳焰器降低NO_x试验研究.

三次空气分级天然气燃烧器中稳焰器可以大幅度降低不完全燃烧热损失,但却提高了 NOx 的排放浓度,其最佳工况点为天然气管与一次风管、二次风管和三次风管的流通 面积之比为1 ∶5. 51 ∶1. 92 ∶3. 97 及天然气管与一次风管、二次风管的插深之比为 1 ∶0. 75 ∶0. 208 3 。试验表明,稳焰器的安装位置影响NOx 的排放量,烟气中NOx 最 低排放量为24. 26 mg/ m3 。

半导体激光直接倍频的488nm蓝光激光器

利用波导型准相位匹配周期极化反转铌酸锂（PPLN）晶体直接倍频波长为976nm的连续半导体激光二极管，在最佳晶体工作温度（28℃）下，获得了波长为488nm的连续蓝光输出，最大输出功率大于20mW。所用的晶体尺寸为8mm×1．4mm×1mm，波导截面为4．5μm×3．5μm，极化周期为5．2μm。研究了波导型周期极化反转铌酸锂晶体的倍频效率与温度的关系，与普通的周期极化反转铌酸锂相比，倍频效率与温度关系的敏感度较低。同时，由于晶体可以在室温下工作，简化了加温与温控部件，提高了整机的工作效率。在此实验的基础

池塘养殖环境下滇池金线鲃仔稚鱼的食性转化与生长

下载PDF阅读器2008年3月-2009年3月,把35 000尾滇池金线鲃(Sinocyclocheilus grahami)仔稚鱼分别饲养于3个池塘进行对比饲养研究.密度分别是:P1为208尾/m3;P2为167尾/m3;P3为41尾/m3.饲养期间采用相同的管理措施.每天数据收集采用:在仔鱼孵化后的5～16d的每天8:00和18:00从每个育苗池中捞取5～8尾仔鱼进行测量分析.仔鱼开口30d后,每月从培育池各捞取15尾,测量全长和体重.研究发现,滇池金线鲃仔鱼的开口时间为孵化后的5 d,混合营养期3～5d,这与初孵的演池金线鲃仔鱼的全长和卵黄囊较大有关.在容易造成仔鱼大量死亡的混合营养期,通过采用浆状物-轮虫-人工混合饲料的方式,大幅度提高了滇池金线鲃仔稚鱼的存活率,孵化一年后的滇池会线鲃稚鱼存活率可达82.7%.经过一周年的饲养,滇池金线鲃稚鱼的体重由(0.027±0.01)g(0.003～1.22)增长到(8.83±0.54)g (3.7～16.7),月均增重为0.73 g;全长由(15.8±0.30)mm (11.5～20.0)增长到(91.6±1.67)mm (75.0～16.0),全长月均增长6.32 mm.滇池金线鲃稚鱼全年全长的增长呈线性相关,y=9.82+7.05month(R2=0.9891);全年体重的增长呈指数关系,y=0.0158month2.54(R2=0.9939).

菲氏叶猴牙齿与颅长的相关性研究

菲氏叶猴较大的个体出现了相对小的门齿. 而臼齿, 特别是M3的面积则随个 体的增大而增大, 这种与菲氏叶猴在摄取叶类食物过程中臼齿嚼磨起主要作用 相关。整个食物嚼磨过程中, 菲氏叶猴的下颌似乎主要作左右(颊一舌侧)方向 运动。菲氏叶猴芽齿与个体大小的相关关系更接近于橄榄疣猴。图5表2参20

垃圾焚烧飞灰旋风炉高温熔融试验研究

在1台75 t/h旋风炉上进行垃圾焚烧飞灰高温熔融处理试验,采用煤粉作为辅助燃料,飞灰与煤粉质量比为2:8,试验结果表明,在旋风炉内温度达到1 450℃,飞灰中的二恶英能够被彻底分解,急冷熔渣、尾气及静电除尘器捕集灰中二恶英浓度很低,分别为2~3 ng-TEQ/kg、0.033 ng-TEQ/m3以及23~26 ng-TEQ/kg,远低于现行国际、国内二恶英排放标准,可以安全排放。对急冷熔渣、尾部飞灰进行重金属浸出实验研究,发现重金属浸出量远低于国家危险废物浸出标准,说明急冷熔渣及尾部飞灰可作为建筑材料循

复合垂直流人工湿地在奥林匹克森林公园龙型水系的应用

介绍了北京奥林匹克森林公园人工湿地系统的设计,跟踪调查了复合垂直流人工湿地在龙型水系水质改善中的功能和效果。复合垂直流人工湿地系统占地面积为41 500 m2,处理水量为2 600 m3/d的污水处理厂尾水(再生水)和20 000 m3/d的主湖循环水。一年多的运行表明,该系统与其他生态工程的协同作用有效地改善了公园内的龙型水系水质,特别是在奥运期间,在保证了奥运主湖水质良好的同时,为运动员和观众提供了优美的休息环境,取得了良好的生态、环境、经济和文化等综合效益。

鲢鱼放养控制北京城市河湖水华试验研究

介绍了利用鲢鱼控制北京城市河湖水华的试验研究,通过不同放养密度(0、17、51和103 g/m3)的现场围隔试验,对鲢鱼放养对水体水质和浮游生物的影响进行了分析。研究结果表明,鲢鱼放养使有鱼围隔中浮游生物量极低,从而使浮游植物基本不受浮游动物影响,而直接受鱼类影响;同时,中等密度(51 g/m3)的鲢鱼放养使水华蓝藻和微小藻的生长均得到了有效抑制,使藻类生物量最低。

西江肇庆江段鱼类早期资源时空分布特征研究

2006年5～7月对西江肇庆江段鱼苗的时空分布进行了研究,结果表明:5月17日及6月1日前后该江段有苗汛发生,鱼苗密度平均值为0.83尾/m3,最大值为7.9尾/m3;鱼苗密度昼夜分布有显著性差异(n=26,P<0.01),夜间的密度比白天的高;鱼苗的种类在昼夜分布上也有明显差异,四大家鱼鱼苗的分布主要集中于夜间;鱼苗的分布具空间差异性,近岸的鱼苗密度高于江中央的。

膜生物反应器稳定运行的操作条件优化研究

膜污染是限制膜生物反应器(MBR)应用和发展的瓶颈。为此,选择污泥浓度、膜通量(JV)、曝气量(Q气)、抽吸/停抽时间(tR/tS)、反应器升流区与降流区面积之比(Ar/Ad)进行正交试验,开展了MBR操作条件的优化研究。结果表明,溶解性胞外多聚物是导致膜污染形成和发展的主要物质,其中蛋白质/多糖值与膜压差上升速率存在线性关系;较佳的操作参数为:MLSS=7g/L,JV=10 L/(m2.h),Q气=6 m3/h,tR/tS=4 min/1 min,Ar/Ad=1.7。