Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit

Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is important in the human genome project. Here an automated fluorescent method that can rapidly and accurately genotype multiplex known SNPs was developed by using a homemade kit, which has lower cost but higher resolution than commercial kit. With this method, oncogene K-ras was investigated, four known SNPs of K-ras gene exon 1 in 31 coloerctal cancer patients were detected. Results indicate that mutations were present in 8(26%) of 31 patients, and most mutations were localized in codon 12. The presence of these mutations is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. (C) 2003 Elsevier B.V. All rights reserved.

用A-PAGE鉴定谷子遗传多样性

蛋白质是结构基因编码的产物,其组成不因环境条件的改变而改变,可以作为对其编码的结构基因的标记.大量研究表明,禾谷类作物种子贮藏蛋白具有相当大的多态性,不同品种贮藏蛋白的电泳谱带存在较大差异,因此提供了一种表征基因型差异的有效而简便的方法