Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit

Accurate and fast genotyping of single nucleotide polymorphisms (SNPs) is important in the human genome project. Here an automated fluorescent method that can rapidly and accurately genotype multiplex known SNPs was developed by using a homemade kit, which has lower cost but higher resolution than commercial kit. With this method, oncogene K-ras was investigated, four known SNPs of K-ras gene exon 1 in 31 coloerctal cancer patients were detected. Results indicate that mutations were present in 8(26%) of 31 patients, and most mutations were localized in codon 12. The presence of these mutations is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. (C) 2003 Elsevier B.V. All rights reserved.

教养方式、亲子关系对青少年给予母亲情感支持的影响

Emotional support, as an important form of social supports, has great impact on the life of both caregivers and receivers. Many studies on adult children and old parents indicate that emotional support is closely related to parents’ well-being, and the relation quality between old parents and adult children is one of the factors influencing adult children’s support offering. However, the study on adolescents’ emotional support to their parents is quite rare. What’s more, it has been found that emotional support skills children have learnt at home have life-time influence on children’s response to other’s emotional needs. It is assumed that to some extent the emotional support styles between parents and adolescents are relatively stable, and they are related to the care children give to their parents after they advance to old age. Hence, it is necessary to study the emotional support of adolescent in order to at last improve the well-being of old parents. Totally speaking, the aim of this study is to explore the relation between parenting styles and adolescents’ emotional support offering, as well as the effects of mother-child relationships. In addition, culture is a factor having impact on parenting styles, parent-child relationships and emotional support. So we have designed this cross-cultural study to explore cultural differences. The results are as follows: (1) As to Chinese and Indonesian mothers, their Acceptance of adolescents’ behaviors has significant positive predictive effect on children’s emotional support. Besides, the Intimacy and Approval dimensions of mother-child relationships partially mediate the path from parenting styles to emotional support offering, and those effects have no significant difference in China and Indonesia. (2) Chinese and Indonesian mother’s Rejection to adolescents has significant positive predictive effect on Conflict between mothers and adolescents, but has no direct effect on adolescents’ emotional support offering. (3) Adolescents’ support offering has no significant predictive effect on their willingness to help their parents. (4) Adolescents’ support offering has significant predictive effect on their motivation to provide emotional support to their parents. However, the relation between adolescents’ support offering and the willingness to help their parents is not clear. (5) Adolescents have scored high in Acceptance parenting style dimension and relatively low in Rejection dimension, which indicate that mothers in China and Indonesia adopt positive parenting styles. Both Chinese and Indonesian mother-child relationships are good. However, Indonesian adolescents have perceived more Intimacy and Approval from mothers, and there is no significant difference between Chinese and Indonesian adolescents and mothers in the dimension of Conflict.