模拟稳定塘系统对污染指示菌的去除作用

本文研究了模拟稳定塘系统去除污染指示菌如总大肠菌群(TC),粪大肠菌群(FC),粪链球菌(FS)以及异养细菌(HPC)的作用。结果表明,16℃时污水在日进污水10t的模拟稳定塘系统停留9天后,TC与FC的密度下降4个数量级。在日进污水90t,污水理论水力停留时间10天的模拟稳定塘系统进行的全年跟踪监测结果表明,TC与FC密度随各塘依次递减。Ⅳ号塘出水的TC密度下降2.5个数量级,基本上达到了我国GB3838-88中规定的地面水Ⅲ级标准;FC密度下降3个数量级,达到WHO标准的轻污染水平,肯定了稳定塘系统去

Comparison of Gene Expression Profiles of Fenneropenaeus chinensis Challenged with WSSV and Vibrio

Microarray technique was used to analyze the gene expression profiles of shrimp when they were challenged by WSSV and heat-inactivated Vibrio anguillarum, respectively. At 6 h post challenge (HPC), a total of 806 clones showed differential expression profile in WSSV-challenged samples, but not in Vibrio-challenged samples. The genes coding energy metabolism enzyme and structure protein were the most downregulated elements in 6 h post WSSV-challenged (HPC-WSSV) tissues. However, a total of 155 clones showed differential expression in the Vibrio-challenged samples, but not in WSSV-challenged samples. Serine-type endopeptidase and lysosome-related genes were the most upregulated elements in tissues 6 h post Vibrio challenge (HPC-Vibrio). Totally, 188 clones showed differential expression in both 6 and 12 HPC-WSSV and HPC-Vibrio samples. Most of the differentially expressed genes (185/188) were downregulated in the samples of 12 HPC-WSSV, whereas upregulated in the samples at 6 and 12 HPC-Vibrio and 6 HPC-WSSV. The expression profiles of three differentially expressed genes identified in microarray hybridization were analyzed in hemocytes, lymphoid organ, and hepatopancreas of shrimp challenged by WSSV or Vibrio through real-time PCR. The results further confirmed the microarray hybridization results. The data will provide great help for us in understanding the immune mechanism of shrimp responding to WSSV or Vibrio.