天花粉蛋白对体外无血清培养的人早期绒毛细胞滋养层细胞hCG，孕酮分泌的影响

本研究以体外无血清培养的人早期绒毛细胞滋养层细胞和绒毛组织培养为模型，测定了天花粉蛋白对滋养层细胞ｈＣＧ、孕酮分泌的影响，发现体外无血清培养的细胞滋养层细胞分泌的ｈＣＧ在天花粉蛋白浓度为０．１μｇ／ｍｌ即下降５０％，而后下降缓慢，８μｇ／ｍｌ以上方降为零，而绒毛组织培养在天花粉蛋白浓度为０．１μｇ／ｍｌ时ｈＣＧ下降近９０％，而后下降缓慢，至８μｇ／ｍｌ以上降到零，说明体外培养的细胞滋养层细胞中有两个群体，其中一个对天花粉蛋白敏感，另一个不敏感，尽管在形态上很难区别。孕酮的反应则不同，在细胞滋养层细胞和绒毛组织培养中，随天花粉蛋白浓度升高，孕酮分泌均缓慢下降，未出现两阶段下降过程。

Cryopreservation of Kunming mouse oocytes using slow cooling, ultrarapid cooling and vitrification protocols

The cryopreservation of oocytes has been only marginally successful with any of the current protocols, including slow cooling, rapid cooling and vitrification. We wished to test the hypothesis that oocytes from a single mouse strain would freeze successfully by 1 of the 3 mentioned protocols. Unfertilized Kunming mouse oocytes obtained 14 h after PMSG/hCG administration were randomly assigned to be cryopreserved after slow cooling, ultra rapid cooling and vitrification. Oocytes were thawed by straws being placed into 37 degrees C water, and their morphological appearance and in vitro fertilization capability were compared with that of oocytes that had not undergone cryopreservation. Survival of oocytes was indicated by the absence of darkened ooplasm or by broken membranes or zona pellucida. Functional integrity was evaluated by the formation of a 2-cell embryo after IVF. Survival rate of slow cooled oocytes did not differ from that seen in vitrified oocytes (55.1 vs 65.9%) but was significantly lower in the rapidly cooled oocytes (24.2%; P<0.01). The results of NF of slow cooled and vitrified oocytes were similar to those of the control group (72 and 73 vs 77%; P>0.05). It appears that Kunming mouse oocytes can be successfully cryopreserved using the slow cooling method with 1,2-propanediol and vitrification, which contains both permeating and nonpermeating cryoprotectants. (C) 1997 by Elsevier Science Inc.

一种改良的双峰蛇血浆促黄体素放射免疫分析方法

研究用单峰驼LH标准品CamLH、CamLH NZY 01和人的LH(hLH)或人绒毛膜促性腺激素(hCG)作为标准品,~(125)I-hLH或~(125)I-hCG作为标记品,抗hLH或hCG血清作为抗血清,组成了不同的双峰驼血浆促黄体素放射免疫分析的组合系统,通过血浆样品稀释曲线,比较了hLH和hCG抗原抗体与CamLH,CamLH NZY 01的交叉反应,选择出测定双峰驼血浆促黄体素交叉反应较强的异种抗原抗体反应系统,即用CamLH作为标准品,~(125)I-hLH作为标记品,抗hLH血清作为抗血清组成的测定系统,并通过血浆中添加标准参考品后的回收实验及母驼垂体兴奋实验等证明了该方法的特异性、准确性和可靠性。说明该方法可以用于测定双峰驼血浆促黄体素的浓度,是研究双峰驼生殖内分泌学的可靠手段之一。

幼猴和成年猴颗粒细胞离体下甾体形成的激素调节

用无血清培养技术研究表明: 幼龄和成年恒河猴颗粒细胞在离体下都能对人FSH、环-AMP、FK和GnRH类似物产生反应, 明显增加孕酮和雌激素的产生; hCG能够促进FSH刺激幼猴和年猴颗粒细胞增加孕酮的产生; 在培液中加入DES明显增加FSH刺激孕酮和雌激素产生的效应; 在缺少雌性激素底物的情况下, 猴壳粒细胞也能合成可测量的雌激素。图7参21

黑尾近红鲌人工繁殖研究

报道了2001～2003年对长江上游特有鱼类黑尾近红鲌开展人工繁殖试验的结果。黑尾近红亲鱼来源于四川泸州长江上游一级支流龙溪河。催产剂为促黄体素释放激素类似物(LRH A),绒毛膜促性腺激素(HCG)与鲤鱼脑垂体(PG)。采用一次注射法,并用干法进行人工授精。共进行了23次人工催产,催产成功的占16次,有效的占3次,失败的占4次;共注射雌鱼70尾,其中46尾雌鱼排卵,平均催产率65.71%,受精率8.33%～100.0%,孵化率0.50%～94.71%,获得仔鱼29.3万尾。对亲鱼选择、催产方法、孵化与

蓝藻Anabaenasp.strainPCC7120中一种可诱导的CO_2浓缩机制(CCM)

为了探讨蓝藻Anabaenasp .strainPCC71 2 0在外源无机碳浓度变化时 ,其光合作用对CO2 和HCO-3 的利用特性 ,制备了高CO2 适应细胞 (High CO2 growingcells ,HCG细胞 ) .Anabaenasp .strainPCC71 2 0HCG细胞的生长速率高于LCG细胞 (Low CO2 GrowingCells) ,即在空气中生长的细胞 .当HCG细胞从 5 %CO2 转移到空气中时 ,其碳酸酐酶活性升高 ;它对外源无机碳的表观光合作用亲合力明显提高 ,

An inducible CO2 concentrating mechanism in cyanobacterium Anabaena sp strain PCC7120

In order to define its characteristics of the photosynthetic utilization of CO2 and HCO3- when the ambient inorganic carbon changed, HCG (High-CO2-Growing Cells) of cyanobacterium Anabaena sp. strain PCC7120 were prepared. The growth rate of HCG was higher than that of LCG (low-CO2-growing cells, i.e. air-growing cells). When the HCG cells were transferred from 5% CO2 to air levels of CO2 , a series of changes took place: its carbonic anhydrase activity as well as its photosynthetic affinity to the external inorganic carbon significantly increased; the number of the carboxysomes, which is one of the most important components of CCM in cyanobacteria also increased. These facts indicated that the CCM activity of Anabaena PCC 7120 was induced. When the pH in the medium increased from 6 to 9, the photosynthetic affinity to external inorganic carbon of both HCG and LCG declined, while the apparent photosynthetic affinity to external CO2 increased. In the light of these findings, this inducible CCM in cyanobacteria provided a good model for the study of the photosynthetic Ci utilization in the phototrophic microoganisms.

Observations on the spawning behavior of artificially matured Japanese eels Anguilla japonica in captivity

Spawning behavior of artificially matured Japanese eels Anguillo japonica in captivity was investigated using a DVD Video image system. Following a routine hormone treatment technique for this fish, female eels were artificially matured by weekly intramuscular injections of salmon pituitary extracts (SPE) at a dosage of 40 mg kg(-1) BW for a total of 7-11 doses to induce ovarian maturation, while male eels received weekly intramuscular injections of human chorionic gonadotropin (HCG) at a dosage of 1000 IU kg(-1) BW for a total of 6-11 doses at 18 degrees C to induce testicular maturation in a separate aquarium. In this experiment, three pairs of such hormone-treated matured eels were acclimatized in seawater in 1.5 m(3) experimental aquaria with or without shelters at 20 degrees C for 24 h. Twenty four hours after the acclimatization terminated, the females received SPE injections to boost maturation and ovulation. Twenty four hours following these injections, the females received injections of HCG (1000 IU per fish, HCG injection) and 17 alpha-hydroxyprogesterone (2 mg per fish) to induce ovulation, while males were given HCG injections (1000 IU per fish, HCG injection) to induce spermiation. Video taping started after the 24 h acclimatization terminated and last for a total of 96 h. Before the HCG injections, both sexes were inactive, staying on the bottom or in shelters if available. Following these HCG injections, they became active and frequently left the bottom swimming in the water column. During the 24 h following HCG injections, activity accounted for 67% and 45% of the total activity in no shelter treatment for females and males, respectively, in comparison with 77% and 78% in shelter treatment. Activity was significantly more pronounced during this phase than during other phases for each sex in either shelter treatment. Egg release and sperm ejection occurred in the water column around the time eels' activity reached peaks. Eels either returned into the shelters or stayed motionlessly on the bottom of the aquaria after egg release and sperm ejection. Eight out of nine (89%) females in no shelter treatment spontaneously released eggs with a total of 11 batches 14-18 h following HCG injections, in contrast with four out of nine (44%) females releasing eggs for 4 batches 16-20 h in shelter treatment. Males arrived at activity peaks 11-13 h following HCG injections in no shelter treatment, 2-4 h ahead of the females (14-16 h), in comparison with 8-11 h in shelter treatment with 5-6 h ahead of the females (14-17 h). Courtship behavior indicative of spawning such as pairing, chasing and touching bodies was not observed in the eels in this study. However, on many occasions, eels of both sexes (male-female or female-female) were found to "cruise together" in water column for a short time period or frequently come together prior to releasing eggs and ejecting sperm, suggesting the possibility of group mating in artificially matured Japanese eels. (c) 2007 Elsevier B.V. All rights reserved.