Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization

Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.

东大别山晚中生代主剪切带及伸展构造研究

Dabie shan lies between Northchina crust and Yangzi crust, which is the result of the collisional orogenen in Triassic period. The biggest area of UHP metamorphic zone have been found in Dabie Shan, which have been verified formed during the course of collision and extrusion after orogenic activity. The Dabie shan is divisioned into four parts, which are North Huaiyang metamorphic zone, North Dabie complex zone, South Dabie ultra-high pressure metamorphic zone and Susong metamorphic zone. Extension structure of late Mesozoic is the key to explain the intrusion and outcrop of UHP metamorphic rocks in Dabie Shan. During the course of structure evolution of the Dabie shan in late Mesozoic period, Luotian dome was formed with the old gneiss lifting from the core of the Dabie shan. There are four enormous ductile zone circled Luotian dorm. Xiaotian-mozitan shear zone is the limit of North Huaiyang metamorphic zone and North Dabie complex zone; Shuihou-wuhe shear zone is the limit of North Dabie complex zone and South Dabie ultra-high pressure metamorphic zone; Taihu-mamiao shears zone is the limit of South Dabie ultra-high pressure metamorphic zone and Susong metamorphic zone and Susong-Qingshuihe shear zone is the south limit of Susong metamorphic zone; the old stress at Dabie shan in late Mesozoic was about 90MPa through the experiment of transmission electricity microscope. The main four ductile shear zone of Dabie shan all have the characteristic of detachment, Xiaotian-mozitan shear zone detached to NNE, the detachment direction of Shuihou-wuhe shear zone and Taihu-mamiao shears zone is SSE, and Susong-Qingshuihe shear zone is SW. The finite strain measurement show that Xiaotian-mozitan shear zone have experienced detachment which was more than 50km, and the detachment of Susong-Qingshuihe shear zone was more than 12km in late Mesozoic; the Flin parameter of Shuihou-wuhe shear zone is much smaller than 1(0.01-0.1), which show that this shear zone was squeezed when it was formed and the initiative function of Luotian granite intrusion during the course of detachment. The Flin parameter of Taihu-mamiao shears zone is above 1(1.1) and Susong-Qingshuihe shear zone is much more than 1(7.6), which show that they are formed in the state of extension at the beginning. These all Flin parameter imply a transition from pure shear to simple shear of the south three shear zone circling Luotian dome from north to south. The rock group analysis show that the rocks inside shear zone encountered middle or high temperature metamorphic activity. The single mineral ~(40)Ar/~(39)Ar age of the main shear zone at Dabie shan show that the three shear zone north to Luotian dome were formed about 190Ma.Taihu-mamiao shear zone was the earliest, Susong shear zone was later than former, and Shuihou-wuhe sheanaone was the latest. They were all the chanel of returning of UHP metamorphic rocks, so they all representative the returning age of UHP metamorphic rocks. The final outcrop of these UHP metamorphic rocks was due to the detachment aroused by the enormous magma intrusion. The biotite age of deformed rocks in Susong-Qingshuihe shear zone is in average 126Ma, and the age of Xiaotian-mozitan is about 125Ma, which is in the same time or a little later than magma intrusion of Luotian dome, and imply that granite intrusion of late Mesozoic in Dabie orogenen is the reason of the detachment.

Combined effect of surface tension, gravity and van der Waals force induced by a non-contact probe tip on the shape of liquid surface

Aiming at understanding how a liquid film on a substrate affects the atomic force microscopic image in experiments, we present an analytical representation of the shape of liquid surface under van der Waals interaction induced by a non-contact probe tip. The analytical expression shows good consistence with the corresponding numerical results. According to the expression, we find that the vertical scale of the liquid dome is mainly governed by a combination of van der Waals force, surface tension and probe tip radius, and is weekly related to gravity. However, its horizontal extension is determined by the capillary length.

Multi-scale analysis of AFM tip and surface interactions

Thoroughly understanding AFM tip-surface interactions is crucial for many experimental studies and applications. It is important to realize that despite its simple appearance, the system of tip and sample surface involves multiscale interactions. In fact, the system is governed by a combination of molecular force (like the van der Waals force), its macroscopic representations (such as surface force) and gravitational force (a macroscopic force). Hence, in the system, various length scales are operative, from sub-nanoscale (at the molecular level) to the macroscopic scale. By integrating molecular forces into continuum equations, we performed a multiscale analysis and revealed the nonlocality effect between a tip and a rough solid surface and the mechanism governing liquid surface deformation and jumping. The results have several significant implications for practical applications. For instance, nonlocality may affect the measurement accuracy of surface morphology. At the critical state of liquid surface jump, the ratio of the gap between a tip and a liquid dome (delta) over the dome height (y(o)) is approximately (n-4) (for a large tip), which depends on the power law exponent n of the molecular interaction energy. These findings demonstrate that the multiscale analysis is not only useful but also necessary in the understanding of practical phenomena involving molecular forces. (c) 2007 Elsevier Ltd. All rights reserved.

透红外大尺寸氧氟化物玻璃研究

锗酸盐和氟铝酸盐透红外玻璃材料作为窗口或头罩材料具有广泛的应用前景。结合锗酸盐玻璃含有少量水的问题，在成分中引入氟化物，实验表明：随氟化物含量增加．玻璃中羟基含量逐渐降低。同时通过对熔制温度的调整，获得了不合羟基的红外玻璃。针对氟铝酸盐玻璃在冷却过程中易析晶的问题，在氟化物组分中加入少量重金属氧化物TeO2，得到析晶性能好的氧氟铝酸盐玻璃。同时给出了两类红外材料的一些物理、化学性质。

Characterization of InAs quantum dots on lattice-matched InAlGaAs/InP superlattice structures

Self-assembled InAs quantum dots (QDs) in an InAlGaAs matrix, lattice-matched to InP substrate, have been grown by molecular beam epitaxy (MBE). Transmission electron microscopy (TEM), double-crystal X-ray diffraction (DCXRD) and photoluminescence (PL) are used to study their structural and optical properties. In InAs/InAlGaAs/ InP system, we propose that when the thickness of InAs layer deposited is small, the random strain distribution of the matrix layer results in the formation of tadpole-shaped QDs with tails towards random directions, while the QDs begin to turn into dome-shaped and then coalesce to form islands with larger size and lower density to release the increasing misfit strain with the continuous deposition of InAs. XRD rocking curves showing the reduced strain with increasing thickness of InAs layer may also support our notion. The results of PL measurements are in well agreement with that of TEM images. (C) 2004 Elsevier B.V. All rights reserved.

Shape evolution of Ge/Si(001) islands induced by strain-driven alloying

The shape evolution of Ge/Si(001) islands grown by ultrahigh vacuum chemical vapor deposition were investigated by atomic force microscopy at different deposition rates. We find that, at low deposition rates, the evolution of islands follows the conventional pathway by which the islands form the pyramid islands, evolve into dome islands, and dislocate at a superdome shape with increasing coverage. While at a high deposition rate of 3 monolayers per minute, the dome islands evolve towards the pyramids by a reduction of the contact angle. The presence of the atomic intermixing between the Ge islands and Si substrate at high deposition rate is responsible for the reverse evolution. (C) 2001 American Institute of Physics.

Characteristics of shallow gas hydrate in Okhotsk Sea

Multidisciplinary field investigations were carried out in Okhotsk Sea by R/V Akademik M.A. Lavrentyev (LV) of the Russian Academy of Sciences (RAS) in May 2006, supported by funding agencies from Korea, Russia, Japan and China. Geophysical data including echo-sounder, bottom profile, side-scan-sonar, and gravity core sample were obtained aimed to understand the characteristics and formation mechanism of shallow gas hydrates. Based on the geophysical data, we found that the methane flare detected by echo-sounder was the evidence of free gas in the sediment, while the dome structure detected by side-scan sonar and bottom profile was the root of gas venting. Gas hydrate retrieved from core on top of the dome structure which was interbedded as thin lamination or lenses with thickness varying from a few millimeters to 3 cm. Gas hydrate content in hydrate-bearing intervals visually amounted to 5%-30% of the sediment volume. This paper argued that gases in the sediment core were not all from gas hydrate decomposition during the gravity core lifting process, free gases must existed in the gas hydrate stability zone, and tectonic structure like dome structure in this paper was free gas central, gas hydrate formed only when gases over-saturated in this gas central, away from these structures, gas hydrate could not form due to low gas concentration.

The Obduction of Equatorial 13 degrees C Water in the Pacific Identified by a Simulated Passive Tracer

The obduction of equatorial 13 degrees C Water in the Pacific is investigated using a simulated passive tracer of the Consortium for Estimating the Circulation and Climate of the Ocean (ECCO). The result shows that the 13 degrees C Water initialized in the region 8 degrees N-8 degrees S, 130 degrees-90 degrees W enters the surface mixed layer in the eastern tropical Pacific, mainly through upwelling near the equator, in the Costa Rica Dome, and along the coast of Peru. Approximately two-thirds of this obduction occurs within 10 years after the 13 degrees C Water being initialized, with the upper portion of the water mass reaching the surface mixed layer in only about a month. The obduction of the 13 degrees C Water helps to maintain a cool sea surface temperature year-round, equivalent to a surface heat flux of about -6.0 W m(-2) averaged over the eastern tropical Pacific (15 degrees S-15 degrees N, 130 degrees W-eastern boundary) for the period of integration (1993-2006). During El Nino years, when the thermocline deepens as a consequence of the easterly wind weakening, the obduction of the 13 degrees C Water is suppressed, and the reduced vertical entrainment generates a warming anomaly of up to 10 W m(-2) in the eastern tropical Pacific and in particular along the coast of Peru, providing explanations for the warming of sea surface temperature that cannot be accounted for by local winds alone. The situation is reversed during La Nina years.

南海大陆边缘地壳结构与盆地形成机制--以珠江口－琼东南盆地为例

South China Sea is located in the convergence of Eurasian plate, the Pacific Ocean plate and Indian Ocean-Australia plate. The total area is about 3,500,000 km2, the geologic structure is complicated, and the structure line cut off reciprocal is the marginal sea taking form by that the seafloor spreads during the middle Oligocene. South China Sea continental margin have developed more than 10 large oil-gas bearing basins and a number of medium-small sized basins. These basins contain abundant mineral resources such as oil & gas. The marginal deepwater area in the north part of South China Sea has become our country’s strategic energy prospecting frontier. The deepwater area of Zhujiangkou and Qiongdongnan basins is the research target in this thesis. The thesis studied deep structure and the earth dynamics of the north part of South China Sea margin, and these researches provide scientific basis for oil-gas resources strategic investigation and valuation in deepwater sea area of north part slope of South China Sea. In order to develop the research of rebuilding velocities and density architecture of earth shell in region of interest, in marginal deepwater area in the north part of South China, we adopted 14 long-cable seismic reflection profile data of 3556.41 kilometers in total, the gravity measurement data along profiles (3851.44 kilometers in total), the magnetic observation along profiles (3838.4 kilometers in total) and depth measurement along profile, the logging data of 11 wells in project, the interpreted fault parameter and preexisting geologic and geophysical research achievement. This thesis has carried out concretely studying research as follows: 1. Overlay-velocity data sampling and analysis, interval velocity calculation, time-depth conversion, model building of earth shell velocity and layering character of earth shell are studied on 14 deep sections. Velocity structure in region of interest has revealed: Changchang is the sag with thinnest crust in Qiongdongnan basin; the sedimentary thickness lowers gradually from north to south, and the thickness change from west to east is milder. The sags’ sedimentary velocities in Qiongdongnan basin have obvious demarcation. The velocity of the 8000 meters sedimentary rocks is 4700 m/s in Shunde sag and Baiyun sag, and is the lowest; at that depth, the velocity very different in Liwan sag and Baiyun sag, which is about 800m/s. 2. Extracting gravity data and building of initial crust density model along the section; With Bouguer gravity anomaly data as constraint, revising density distributes of initial model, and building the crust density model. 3. With crust velocity and density as constraint, correcting the effect of thermobaric field and constructing constitution structure of rock in region of interest. By this research, we known that rocks in Zhujiangkou upper crustal layer are chiefly granite-gneiss, quartzite, granodiorite and basalt, however, rocks in Qiongdongnan basin upper earth shell are chiefly composed of granite-gneiss, quartzite, granodiorite, diorite and basalt. 4. Synthetically crust velocity and density structure, gaining expanding factor on crust and entire crust along section. The result is indicated: the expanding factor in every sag rises from northwest to southeast, which have reflected thinning characteristic of crust from continent to ocean. Intra-crustal deformation degree in Changchang and Ledong-Lingshui sag is bigger than that in Songnan-Baodao sag. Entire crust extension factor in Changchang and Songnan-Baodao sag is greater than that in Ledong-Lingshui sag, which can make an explanation of frequently event and longer heating process in middle-east of Qiongdongnan basin. 5. Synthesize multidisciplinary information to discuss the earth dynamics significance of discordogenic seismic profile in deepwater area of Zhujiangkou and Qiongdongnan basins.

松辽盆地长岭断陷火山岩储层识别与勘探目标评价

Changling fault depression is the biggest fault subsidence in south of Songliao Basin. In its Lower Cretaceous Yingcheng and Shahezi formations developed thick source rocks of deep lake facies and developed poly-phase volcanic rock reservoirs as well. In recent years, significant breakthroughs have been obtained in hydrocarbon exploration of volcanic rock reservoir in the different fault depressions in Songliao basin. Lately, I have been involved in hydrocarbon exploration in the Changling rift depression, especially volcanic rock reservoirs and exploration targets research, participating in the deployment of well Yaoshen 1 which gained over 40 × 104m3 natural gas flow. As quick changes of lithology and facies in Changling area in the south of Songliao basin, and the volcanic rock interludes distribution in continental clastic rock and shale in 3D space, so the identification of volcanic rock types and distribution become a difficult problem. Thus, based on the integrated research of the wild outcrop observation, gravity, magnetic and seismic data, geophysical logging, drilling and coring, laboratory test, this paper carried out the reservoir identification, description and prediction of volcanic rocks in Changling fault depression. In this area, this paper analyzed the volcanic rocks litho-facies, the eruption period, and characteristics of cycles. At the same time, tried to know how to use logging, seismic data to separate volcanic rocks from sandstone and shale, distinguish between volcanic reservoir and non-reservoir, distinguish between intermediate-basic and acidic volcanic rocks, and how to identify traps of volcanic rocks and its gas-bearing properties, etc. Also it is summarized forming conditions and distribution of traps, and possible gas-bearing traps were optimized queuing management. Conclusions as follows: There are two faulted basements in Changling fault depression, granite basement in the southeast and upper paleozoic epimetamorphic basement in the northwest. The main volcanic reservoirs developed in Yingcheng period, which was the intermediate-basic and acidic volcanic eruptions, from the south to north by the intermediate-basic to acid conversion. The volcanic vents are gradually young from south to north. According to information of the re-processing 3D seismic data and gravity-magnetic data, the large volcanic vent or conduit was mainly beaded-distributed along the main fault. The volcanic rocks thickness in Yingcheng formation was changed by the deep faults and basement boundary line. Compared with the clastic rocks, volcanic rocks in Changling area are with high resistance and velocity (4900-5800), abnormal Gamma. All kinds of volcanic rocks are with abnormal strong amplitude reflection on the seismic stacked section except tuff. By analyzing the seismic facies characteristics of volcanic rocks, optimizing seismic attributes constrained by logging, using seismic amplitude and waveforms and other attributes divided volcanic rocks of Yingcheng formation into four seismic zones in map. Currently, most volcanic gas reservoirs are fault-anticline and fault-nose structure. But the volcanic dome lithologic gas reservoirs with large quantity and size are the main gas reservoir types to be found.

藏东南巨厚松散堆积体发育条件、成灾模式及防治对策——以川藏公路然乌至鲁朗段为例

The unique geologic, geomorphic and climatic conditions of southeast Tibet have made the region to develop the multi-style and frequently occurring geologic hazards, especially the collapses and landslides and debris flows along the section of Ranwu-Lulang in Sichuan-Tibet highway. However, most of those geologic hazards have close relationship with the loose accumulations. That is, the loose accumulations are the main carrier of most geologic hazards. Thereof, the huge-thick accumulations along the highway is regarded as the objective in the thesis to study the geologic background, hazarding model and mitigation methods comprehensively, based on the multi-disciplinary theories and former materials. First of all, in the paper, based on field engineering geologic investigations, the genetic type and the characteristics of spatiotemporal distribution of the huge-thick loose accumulations along the highway, have been analysized from the factors of regional geology and geomorphy and climate, as well as the coupling acting of those factors with inoculation and eruption of the loose accumulations geologic hazards. The huge-thick loose accumulations has complex genetic types and specific regulations of spatiotemporal distribution, closely controlled by the outer environment of the region. The accumulations are composed of earth and boulder, with disorder structure and poor sorting, specific forming environments and depositing conditions. And its physical and mechanic properties are greatly distinguished from rock and common earth inland. When Sichuan-Tibet highway was firstly constructed along the north bank of Purlung Tsangpo River, the huge-thick loose accumulations was cut into many high and steep slopes. Through the survey to the cut-slopes and systematic investigation to their failures, the combination of height and angle of the accumulations slope has been obtained. At the same time, the types of genetic structure of those cut-slopes are also analysized and concluded, as well as their failure models. It is studied in the paper that there are piaster, duality, multielement and complexity types in genetic structure, and rip-dump-repose, rip-shear-slip and weathering-flake types in failure models. Moreover, it is briefly introduced present engineering performance methods and techniques dealing with the deformation and failure of the accumulations cut-slope. It is also suggested that several new techniques of slope enforcement and the method of landslide and rockfall avoiding should be applied. The research of high and steep cut-slope along the highway has broadened the acknowledgement of the combination of cut-slope height and angle. Especially, the dissertation also has made the monographic studies about the geologic background and hazarding models and prevention methods of some classic but difficult accumulations geologic hazards. They are: (1) Research of the engineering geologic background of the 102 landslide group and key problems about the project of tunnel. The 102 landslide group is a famous accumulational one composed of glacial tills and glaciofuvial deposit. The tunnel project is a feasible and optional one which can solve the present plight of “sliding after just harnessing” in the 102 section. Based on the glacial geomorphy and its depositing character, distribution of seepage line, a few drillhole materials and some surveying data, the position of contact surface between gneiss and accumulations has been recognized, and the retreating velocities of three different time scales (short, medium and long term) have been approximately calculated, and the weathering thickness of gneiss has also been estimated in the paper. On the basis of above acknowledgement, new engineering geomechnic mode is established. Numerical analysis about the stability of the No.2 landslide is done by way of FLAC program, which supplies the conclusion that the landslide there develops periodically. Thereof, 4 projects of tunnel going through the landslide have been put forwards. Safety distance of the tunnel from clinohefron has been numerically analysized. (2) Research of the geologic setting and disaster model and hazard mitigation of sliding-sand-slope. From the geologic setting of talus cone, it is indicated that the sliding-sand-slope is the process of the re-transportation and re-deposit of sand under the gravity action and from the talus cone. It is the failure of the talus cone essentially. The layering structure of the sliding-sand-slope is discovered. The models of movement and failure of the sliding-sand-slope has been put forwards. The technique, “abamurus+grass-bush fence+degradable culture pan”, is suggested to enforcement and green the sliding-sand-slope. (3) Characteristics and hazarding model and disaster mitigation of debris flow. The sources of solid material of three oversize debris flows have been analysized. It is found that a large amount of moraine existing in the glacial valley and large landslide dam-break are the two important features for oversize debris flow to be taken place. The disaster models of oversize and common debris flows have been generalized respectively. The former model better interpret the event of the Yigong super-large landslide-dam breaking. The features of common debris flow along the highway section, scouring and silting and burying and impacting, are formulated carefully. It is suggested that check dam is a better engineering structure to prevent valley from steeply scouring by debris flow. Moreover, the function of check dam in enforcing the slope is numerically calculated by FLAC program. (4) Songzong ancient ice-dammed lake and its slope stability. The lacustrine profile in Songzong landslide, more than 88 meters thick, is carefully described and measured. The Optical Simulated Luminescence (OSL) ages in the bottom and top of the silty clay layer are 22.5±3.3 kaB.P., 16.1±1.7 kaB.P., respectively. It is indicated by the ages that the lacustrine deposits formed during the Last Glacial Maximum ranging from 25ka B.P. to 15ka B.P. The special characteristics of the lacustrine sediment and the ancient lake line in Songzong basin indicated that the lacustrine sediment is related to the blocking of the Purlung Tsangpo River by the glacier in Last Glacial Maximum from Dongqu valley. The characteristics of the lacustrine profile also indicate that the Songzong ice-dammed lake might run through the Last Glacial Maximum. Two dimensional numerical modeling and analysis are done to simulate the slope stability under the conditions of nature and earthquake by FLAC program. The factor of safety of the lacusrtine slope is 1.04, but it will take place horizontal flow under earthquake activity due to the liquefaction of the 18.33 m silt layer. The realign to prevent the road from landslide is suggested.

油气、微生物与砂岩型铀矿形成关系研究——以东胜铀矿床和钱家店铀矿床为例

Sandstone-type uranium deposits are frequently found close to oil fields or uraniferous sandstones contain bitumen or petroleum. However, few evidence has been presented to indicate the association of uranium mineralization with petroleum oxidation. Thus, Dongsheng uranium deposit in Ordos Basin and Qianjiadian deposit in Kailu Basin are taken for examples to solve the puzzle. Integration data from sedimentary petrology, mineralogy, race elements geochemistry, isotope geochemistry and organic geochemistry, the uranium and petroleum sources, and diagenetic paragenesis of the host sandstone are analyzed, and then the genetic relationship between microbes, petroleum and uranium deposits are discussed. The observation under microscope shows that the host sandstone samples from Middle Jurassic Zhiluo Formation in the Dongsheng deposit contained different kinds of metamorphic rock fragments, which should have been derived form outcrops north to this basin. The LREE/HREE ratios of gneiss and amphibolite sampled from outcrops were close to the highest and the lowest LREE/HREE ratios of the sandstones with well-compared chondrite-normalized REE patterns, respectively. So these results consistently indicated that parent rocks of sandstones were mainly contributed from these two kinds of metamorphic rocks. There was very high Th/U ratio for granite gneiss, which was a mainly potential U resource. Hydrocarbon inclusions and adsorbed hydrocarbons are observed under fluorescence microscope in the host sandstone of Dongsheng uranium deposit, suggesting that the sandstones may have been utilized as oil migration pathways. Based on biomarker parameters, it is indicated that the inclusion oils and adsorbed hydrocarbons were marginally mature to mature, and were derived from humic-sapropel type organic matter under poor reducing freshwater to semi-saline environment. The features are similar to those of organic matter extracted from Triassic sandstone and source rock, but are different from that of cretaceous sandstone. Thus, it can be concluded that the inclusion oils and adsorbed hydrocarbons were mainly derived from Triassic lacustrine facies source rock. Observation results under Scanning Electron Microscopy and Electron Microprobe with Energy Spectrum Analysis show that, in Dongsheng area, the main uranium ore mineral is coffinite. The coffinite is intimately intergrown or coexists with pyrite and calcite, thus, the solution during mineralization stage is inferred to be alkaline. The alkaline environment is not favored for uranium to be pre-concentrated by absorption, and then be reduced abiogenetically. δ34S of pyrite and δ13C of calcite indicate that pyrite was formed by bacterial sulfate reduction (BSR) and part of the carbon of calcite has been dirived from oxidation of petroleum, respectively. Additionally, petroleum is found biodegraded. All the lines of evidence consistently indicate that petroleum was involved in uranium mineralization. Coffinite with microbe-like structures is found in the high U sandstone samples and is composed of nanoparticles, indicating the coffinite is biogenic. The conclusion are also supportted by laboratory experiment studies, which have shown that SRB are capable of utilizing U(VI) as the preferred electron acceptor for respiration and reduce U(VI) to U(IV) directly, coupled the oxidaton of organic matter and sulfate reduction. Based on the research results mentioned above, in the Dongsheng area, coffinite is likely to have formed by mixing of brine containing petroleum derived from Triassic with uranium-bearing meteoric water from outcrops north to Ordos Basin. SRB utilize hydrocarbon as carbon source, and directly reduce U(VI) resulting in precipitation of coffinite. The product of metabolism, H2S and CO2, was precipitated as pyrite and calcite during mineralization stage. Petroleum in fluid inclusions and adsorbed type in host sandstone from Lower Cretaceous Yaojia Formation in Qianjiadian uranium deposit, Kailu Basin, are derived from Jurassic Jiufotang Formation in this basin and the uranium mineral consists mainly of pitchblende. The δ34S and δ13C values of pyrite and calcite during mineralization stage indicate SRB have likely degraded petroleum, which is similar to that of Dongsheng deposit. The alkaline environment as indicated by the diagenetic mineral assemblage calcite, Fe dolomite, pyrite and pitchblende deposit suggests that U ore in the Qiangjiajiadian has a similar origin, i.e., direct reduction by SRB. However, less part of pitchblende is intergrown with kaolinite, suggesting the solution during mineralization stage is acidic. The environment is favorable for U(VI) to be adsorded on quartz or other mineral, and then reduced by H2S produced by SRB. Thus, it can be concluded that U(VI) reduction with petroleum oxidation by SRB and other microbes is an important ore-forming mechanism in petroleum-related sandstone-type uranium deposits. The finding is significant in that it provides a theoretical basis for exploration of both uranium and petroleumr.

西天山造山带古生代构造演化与地壳增生—来自花岗岩和蛇绿岩的证据

The Central Asian Orogen Belt (CAOB), which is different from the subductional orogen and the collisional orogen, is known as the most important site of crustal growth in the Phanerozoic, and it has been a ‘hot spot’ for studying the orogenic belts. The Chinese West Tianshan Orogen is occupying the west-southern part of the CAOB and is of great importances to understand the orogenic processes and the continental growth in the Central Asia. The West Tianshan Orogen had undergone complex tectonic evolutional processes in Paleozoic times and large volumes granitic rocks have recorded important information about these processes. Litter is known about Phanerozoic continental growth in the Western Tianshan area so far, compared with the other areas of the CAOB, such as eastern Junggar, western Junggar, Altai and Alakol. The aim of this dissertation is to set up the chronology frame of granitoids in western Tianshan, provide new evidence for the tectonic evolution and discuss the Paleozoic continental growth in this area, on the basis of the studies on the isotopic chronology, major element, trace element and Nd-Sr isotopic geochemistry of granitoids and the isotopic chronology and geochemistry of the ophiolites in this area, especially the Kule Lake ophiolites. 25 precise SHRIMP U-Pb zircon and LA-ICPMS U-Pb zircon ages have been obtained in this dissertation. The granitic rocks in western Tianshan had been formed during two periods: the granitic gneiss with an age of 896Ma, possibly representing the forming age of the Precambrian basement; the granitic rocks with ages varying from 479Ma to 247Ma, recording the Paleozoic orogenic process of western Tianshan. The granitoids in western Tianshan are composed of intermediate-basic rocks, intermediate rocks, intermediate-acid rocks and acid rocks, mainly intermediate-acid rocks and acid rocks. They are mostly granite, granodiorite, quartz syenite and monzodiorite. Different types of granitic rocks are exposed in different tectonic units. The granitoids on the northern margin of the Yili Plate mainly formed in late Paleozoic (413Ma ~ 281Ma), those with ages varying from 413Ma to 297Ma show continental arc affinities and the magnesian calc-alkalic metaluminous diorite of 281Ma display the geochemical characteristics similar to those of granites formed during the post-orogenic period. The granitiods on the southern margin of the Yili Plate include the adakite diorite of 470Ma which was formd by partial melting of thickened lower crust, the post-collisional alkali-feldspar granite of 430Ma, the volcanic arc granite of 348Ma and the Triassic post-collisional granite. The granitoids in the Central Tianshan Plate formed in 479Ma ~ 247Ma, mainly in 433Ma ~ 321Ma. The granitic rocks with ages of 479Ma ~ 321Ma are magnesian calc-alkalic to alkalic rocks with continental arc affinities. A few post-collisional granitoids of 276Ma ~ 247Ma may have inherited the geochemical characteristics of pre-existing arc magma. The granitic rocks in Southern Tianshan (northern margin of the Tarim plate) formed two stages, 420Ma ~ 411Ma and ca. 285Ma. The magnesian calcic to alkalic granites of 420Ma ~ 411Ma may formed during the extension process of the continental margin. The granite of 285Ma includes mostly ferroan calc-alkalic to alkali-calcic rocks with high SiO2 and high alkaline contents, and obviously negative anomaly of Eu, Ba, Sr, P, Ti, similar to the geochemical characteristics of the A-type granite which is formed during post-collisional extension. The Kule Lake ophiolite in southern Tianshan shows the affinity of N-MORB. A SHRIMP zircon U-Pb age of 425±8Ma has obtained for gabbros. Some zircons have given another group of 206Pb/238U age 918Ma, which may indicate the information of the pre-exist old basement rock. The small oceanic basin represented by Kule Lake ophiolite probably developed on the split northern margin of Tarim block. A model for Paleozoic tectonic evolution of the West Tianshan Orogen has been proposed here on the basis of the new results obtained in this dissertation and the previous published data. In Early Cambrian, the Terskey Ocean occurred along the North Nalati fault (NNF), and it separated the Yili plate from the Central Tianshan plate which was probably connected with the Tarim plate. The Terskey Ocean probably subducted towards south under the Central Tianshan plate and towards north under the Yili plate simultaneously. In the early stage of Late Ordovician, the Terskey Ocean had been closed, and the Yili and Central Tianshan plates collided. Meanwhile, extension happened within the joint Central Tianshan and Tarim plates gradually and the Paleo-South Tianshan Ocean had been formed. In Early Silurian, the Paleo-South Tianshan Ocean began to subduct beneath the composite Yili-Central Tianshan plate, which was intruded by volcanic arc granitoids. In Middle Silurian, the Paleo-South Tianshan Ocean, which had reached a certain width, was subducting strongly. And this subduction may have produced voluminous granitoids in the Central Tianshan plate. In the latest stage of Carboniferous, the Paleo-South Tianshan ocean closed, and the Yili-Central Tianshan plate and Tarim plate collided. In Late Cambrian, Paleo-Junggar Ocean occurred to north of the Yili plate; and started to subduct towards south under the Yili plate in Ordovician. This subduction may have produced a magma arc on the northern margin of the Yili plate. In Late Carboniferous, the Paleo-Junggar Ocean had been closed. The Yili-Central and Junggar plates amalgamated together. The West Tianhan Orogen may undergo a post-collisional collapse since Permian. And the magmatic activities may continue to early Triassic. The initial 87Sr/86Sr ration of the granitic rocks in the western Tianshan Mountains varies from 0.703226 to 0.716343, and Nd（t）from -6.50 to 2.03. The characteristics of Sr-Nd isotope indicate that the source of granitic material is not a sole source, which may be produced by mantle-crust magma mixing. In Paleozoic time, lateral growth of the continental crust along active continental margins was dominant, whereas the vertical growth of continental crust resulted from post- collisional mantle derived magmas was not obvious.