A New Designed Two-Dimensional Bifurcation Parallel-Plate Flow Chamber

解决平行平板流槽每次实验只能观测壁面培养细胞受一种剪应力作用的问题。作者在平行平板流槽的基础上,首次提出了一种改进后的流槽--二维平板分叉流槽。通过数值模拟,给出了流体作定常流动时,流速和壁面剪应力的分布。结果发现,利用这种二维平板分叉流槽可以研究壁面培养的细胞在不同大小剪应力作用下的力学行为。该研究结果为流槽的合理设计和使用,并分析剪应力空间分布对内皮细胞的影响有重要实际意义。

Study of Interaction Force between Antigen and Antibody Using Flow Chamber Method

A parallel plate flow chamber was used to study the interaction force between human IgG (immobilized on a chip surface as ligand) and goat anti-human IgG (immobilized on microspheres surface as receptor). First, it was demonstrated that the binding force between the microspheres and the chip surface came from the bio-specific interaction between the antigen and the antibody. Secondly, it was obtained that the critical shear rate to detach microspheres from the chip surface increases with the ligand surface concentration. Finally, two models to estimate the antigen-antibody bond strength considering bonds' positions were proposed and analyzed.

Performance and mechanism of phosphorus removal in an integrated vertical flow constructed wetland treating eutrophic lake water

Phosphorus removal performance and a possible mechanism for the phosphorus removal from an eutrophic lake water were investigated using a medium-scale integrated vertical constructed wetland (combined vertical and reverse-vertical systems) from April, 11, 2001 to September, 28, 2004. Environmental factors affecting phosphorus removal and release profiles were monitored simultaneously under hydraulic loads from 400 to 2000 mm per day. The phosphorus removal rate varied with the environmental conditions. The removal rate for acidic influent water was superior to that for alkaline influent water. The substrate in the wetland chamber acted as a buffer to regulate the pH value of the water sample. As regards the water temperature, no significant differences were observed for the removal rate of total phosphorus (TP) and soluble reactive phosphorus (SRP) between low (lower than 15 degrees C) medium (16-25 degrees C) and high temperature (higher than 26 degrees C) conditions. Under a hydraulic load of 400 mm per day, the removal rate reached over 70%, the highest value achieved in this work. In addition, the highest hydraulic load of 2000 mm/d did not result in the lowest removal rate, as had been expected. After a two-year high hydraulic load test, the removal rate decreased significantly. Phosphorous release from the substrate was examined using a spatial sampling method. Depth profiles of total phosphorus and different states of phosphorus present in the substrate were recorded. This further study demonstrated that binding of phosphorus by iron and calcium might be another major factor in the removal and release of TP and SRP in this wetland system. The distribution of the speciated phosphorus showed that the amount of phosphorus captured in the substrate of the down-flow chamber was significantly higher than that captured in the up-flow chamber, suggesting that the up-flow chamber was the main source of phosphorus release in this constructed wetland.

剪切流动法研究表面固定化配基与目标分子的相互作用力

基于剪切流动腔技术,以微球作为受力载体,设计了一套可用于研究表面固定化配基与目标分子特异性相互作用力的实验和分析方法,并以人免疫球蛋白G (human IgG)和羊抗人免疫球蛋白G(goat anti-human IgG)分别作为模型配基和模型目标分子进行了研究.基于平面Poiseuille层流模型设计了流场参数,以数值计算结果验证了设计的合理性.使用牛血清白蛋白(BSA)作为非特异性对照,判断微球与基片表面的结合力来自配基和目标分子的生物特异性相互作用,并由进一步的目标分子灭活对比实验确认了这一结论.实验观察到微球与基片表面的结合力受到配基面密度的影响,说明发生结合的是多对而非单对蛋白质分子.将95%的微球被剥离时对应的壁面剪切率设定为临界剪切率,由大量实验结果拟合得到了临界剪切率与配基面密度间的定量关系.在受力分析模型中,考虑到多分子的结合,以及分子键位置不同造成的力臂长度的差异,最终计算得到单对配基与目标分子的平均结合力约为342pN.

Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions

Selectin/ligand interactions initiate the multistep adhesion and signaling cascades in the recruitment of leukocytes from circulation to inflamed tissues and may also play a role in tumor metastasis. Kinetic properties of these interactions are essential determinants governing blood-borne cells' tethering to and rolling on the vessel wall. Extending our recently developed micropipette method, we have measured the kinetic rates of E-selectin/ligand interactions. Red cells coated with an E-selectin construct were allowed to bind HL-60 or Colo-205 cells bearing carbohydrate ligands. Specific adhesions were observed to occur at isolated points, the frequency of which followed a Poisson distribution. These point attachments were formed at the same rate with both the HL-60 and Colo-205 cells (0.14 +/- 0.04 and 0.13 +/- 0.03 mum(2) s(-1) per unit density of E-selectin, respectively) but dissociated from the former at a rate twice as fast as did from the latter (0.92 +/- 0.23 and 0.44 +/- 0.10 s(-1), respectively). The reverse rates agree well with those measured by the flow chamber. The forward rates are orders of magnitude higher than those of Fc gamma receptors interacting with IgG measured under similar conditions, consistent with the rapid kinetics requirement for the function of E-selectin/ligand binding, which is to capture leukocytes on endothelial surfaces from flow.

Atomic force microscopy observations of the topography of regenerated silk fibroin aggregations

How fibroin molecules fold themselves and further self-assemble into aggregations with specific structures when the solution concentration increases is the key to understanding the natural silk-forming process of the silkworm. A regenerated Bombyx mori silk fibroin solution was prepared, and serially diluted solutions were coated on aminated coverslips. Atomic force microscopy (AFM) observations of the topography of fibroin molecules revealed a transformation from rodlike aggregations 100-200 nm long to small globules 50 mn in diameter with decreasing concentrations. When the incubation duration increased, the aggregations of fibroin molecules showed a self-assembling process, which was measured with AFM. In particular, after the molecules were incubated for more than 20 min, rodlike micelles formed and were distributed evenly on the surface of the aminated slides. Flow chamber technology was used to study the effect of the shear loading on the topography of the fibroin molecular aggregations. After a shear loading was applied, larger rodlike particles formed at a higher incubation concentration in comparison with those at a lower concentration and were obviously oriented along the direction of fluid flow.

一种新的二维平板分叉流槽的设计

目的 解决平行平板流槽每次实验只能观测壁面培养细胞受一种剪应力作用的问题。方法 作者在平行平板流槽的基础上，首次提出了一种改进后的流槽-二维平板分叉流槽。通过数值模拟，给出了流体作定常流动时，流速和壁面剪应力的分布。结果 结果发现，利用这种二维平板分流槽可以研究壁面培养的细胞在不同大小剪应力作用下的力学行为。结论 该研究结果为流槽的合理设计和使用，并分析剪应力空间分布对内皮细胞的影响有重要实际意义。

T型分叉流槽中前列环素和内皮素分泌的研究

动脉粥样硬化（atherosclerosis）的非随机分布与当地的血流动力环境有关，借助平行平板式平直流槽和以T型分叉流槽为代表的平行平板式异型流槽，可以模拟血管的主要形状特征，首,先在数值模拟的基础上分布了流型特征参数，确定了流的设计尺寸。然后，通过实验研究，探讨流型改变对内皮细胞血管活性物质分泌的影响，发现扩张效应流线偏转和驻点效应使得异型流槽前列环素和内定素的沁泌水平与相同入口雷诺数（Re）条件下的平直流槽分别有降低趋势和显著差异。为进一步研究流型对血管内皮细胞的影响提供了实验数据。

突然扩张流槽中血管内皮细胞表面粘附蛋白表达的研究

动脉粥样硬化的非随机分布与当地的血流动力环境有关，为了研究复杂的流体动力学条件对血和皮细胞生理功能的影响，构建了平行板式平直流槽和突然扩张流槽，通过数值模拟分析了流型的特征，并探讨流型改变对人脐静脉血管内皮细胞表面粘附蛋白表达的影响，发现突然扩张流槽中流动的空间变化使得总体细胞表面粘附蛋白ICAM-1的表达显著高于平直流槽中的均匀定常剪切作用，表明局部流动空间变化的性质可以影响血管内皮细胞的功能。

异形流动腔的数值分析和设计优化

动脉粥样硬化的非随机发生与当地的流体动力环境有关。完全模拟体内血流动力学环境是不可能的。我们的思路是将复杂的血管形态，分解成若干几何因素，尽可能地研究某一几何因素对流场和血管内皮细胞生长的影响。在体外实验中，为了便于在线观测，主要采用流动腔（Flow Chamber）技术。不同于国内外己有的研究，我们主要研究几何因素引起的流型的改变及其对血管内皮细胞生长的影响。本论文主要是用数值方法来研究五种几何因素（扩张、弯曲、驻点、分叉和后向台阶）引起的流型特征。分析各参数的影响，进而优化设计。为在体外进行血管内皮细胞培养实验，研究不同流场条件下由于几何形状改变引起的流场特性改变对内皮细胞生长的影响，提供理论依据。

Enzymatic activities in constructed wetlands and di-n-butyl phthalate (DBP) biodegradation

The bioaccumulation of phthalate acid esters (PAEs) from industrial products and their mutagenic action has been suggested to be a potential threat to human health. The effects of the most frequently identified PAE, Di-n-butyl phthalate (DBP), and its biodegradation, were examined by comparison of two small scale plots (SSP) of integrated vertical-flow constructed wetlands. The influent DBP concentration was 9.84 mg l(-1) in the treatment plot and the control plot received no DBP. Soil enzymatic activities of dehydrogenase, catalase, protease, phosphatase, urease, cellulase, beta-glucosidase, were measured in the two SSP after DBP application for 1 month and 2 months, and 1 month after the final application. Both treatment and control had significantly higher enzyme activity in the surface soil than in the subsurface soil (P < 0.001) and greater enzyme activity in the down-flow chamber than in the up-flow chamber (P < 0.05). In the constructed wetlands, DBP enhanced the activities of dehydrogenase, catalase, protease, phosphatase and inhibited the activities of urease, cellulase and beta-glucosidase. However, urease, cellulase, beta-glucosidase activities were restored 1 month following the final DBP addition. Degradation of DBP was greater in the surface soil and was reduced in sterile soil, indicating that this process may be mediated by aerobic microorgansims. DBP degradation fitted a first-order model, and the kinetic equation showed that the rate constant was 0.50 and 0.17 d(-1), the half-life was 1.39 and 4.02 d, and the r(2) was 0.99 and 0.98, in surface and subsurface soil, respectively. These results indicate that constructed wetlands are able to biodegrade organic PA-Es such as DBP. (c) 2005 Elsevier Ltd. All rights reserved.

Xenobiotics removal from polluted water by a multifunctional constructed wetland

Removal efficiencies on xenobiotics from polluted water in a twin-shaped constructed wetland consisting of a vertical flow chamber with the crop plant Colocasia esculenta L. Schott and a reverse vertical flow one with Ischaemum aristatum var. glaucum Honda, were assessed by chemical analysis and bioassays. After a four-month period of application, removal efficiencies of the applied pesticides parathion and omethoate were 100%, with no detectable parathion and omethoate in the effluent. For the applied herbicides, the decontamination was less efficient with removal efficiencies of 36% and 0% for 4-chloro-2-methyl-phenoxyacetic acid and dicamba, respectively. As shown by toxicity assay with duckweed Lemna minor L., growth retardation may occur if the water treated for herbicide removal is used in irrigation of sensitive cultivars in agriculture or horticulture. In contrast to I. aristatum var. glaucum Honda, the crop C esculenta L. Schott has a high yield in biomass production as a valuable source of renewable energy. (C) 2002 Elsevier Science Ltd. All rights reserved.

Numerical Simulation of the Flow Field and Concentration Distribution in the Bulk Growth of Silicon Carbide Crystals

The physical vapor transport (PVT) method is being widely used to grow large-size single SiC crystals. The growth process is associated with heat and mass transport in the growth chamber, chemical reactions among multiple species as well as phase change at the crystal/gas interface. The current paper aims at studying and verifying the transport mechanism and growth kinetics model by demonstrating the flow field and species concentration distribution in the growth system. We have developed a coupled model, which takes into account the mass transport and growth kinetics. Numerical simulation is carried out by employing an in-house developed software based on finite volume method. The results calculated are in good agreement with the experimental observation.

Three-Dimensional Propagation of the Transmitted Shock Wave in a Square Cross-Sectional Chamber

An investigation into the three-dimensional propagation of the transmitted shock wave in a square cross-section chamber was described in this paper, and the work was carried out numerically by solving the Euler equations with a dispersion-controlled scheme. Computational images were constructed from the density distribution of the transmitted shock wave discharging from the open end of the square shock tube and compared directly with holographic interferograms available for CFD validation. Two cases of the transmitted shock wave propagating at different Mach numbers in the same geometry were simulated. A special shock reflection system near the corner of the square cross-section chamber was observed, consisting of four shock waves: the transmitted shock wave, two reflection shock waves and a Mach stem. A contact surface may appear in the four-shock system when the transmitted shock wave becomes stronger. Both the secondary shock wave and the primary vortex loop are three-dimensional in the present case due to the non-uniform flow expansion behind the transmitted shock.

Numerical Investigation of Toroidal Shock Wave Focusing in a Cylindrical Chamber

In this paper, focusing of a toroidal shock wave propagating from an annular shock tube into a cylindrical chamber was investigated numerically with the dispersion controlled dissipation (DCD) scheme. The first case for an incident Mach number of 1.5 was conducted and compared with experiments for validation. Then, several cases were calculated for higher incident Mach numbers varying from 2.0 to 5.0, and complicated flow structures were observed. The numerical study was mainly focused on two aspects: focusing process and flow structures. The process, including diffraction, focusing, and reflection, is displayed to reveal the focusing mechanism, and the flow structures at different incident. Mach numbers are used to demonstrate shock reflection styles and focusing characteristics.