Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells

Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) effective against HIV-1 and HSV-1 replication. The mechanism of its antiviral activity is not clear. Many believe that it is related to ribosome inactivation. Some RIPs and viral infectio

微囊藻毒素对Hela细胞和Vero细胞的毒性效应

ＭＣＹＳＴ－ＬＲ，ＭＣＹＳＴ－ＲＲ，ＭＣＹＳＴ－ＹＲ稀释后的毒素溶液处理Ｈｅｌａ细胞和Ｖｅｒｏ细胞后得到如下结果：１．３种毒素溶液对培养的Ｈｅｌａ细胞均有明显的抑制作用和毒性作用，其毒性作用与浓度成正比．致死细胞边缘不整齐不规则，细胞呈脱水状态．倒置显微镜下可见细胞变黑无折光性．高浓度毒素溶液（包括ＭＣＹＳＴ－ＬＲ，－ＲＲ和－ＹＲ３个样品）作用细胞２４ｈ后，细胞完全致死脱落，３个样品间无明显差别；２．毒素对Ｖｅｒｏ细胞也具一定的抑制和毒性作用，但不如对Ｈｅｌａ细胞的作用强烈；３．在相同稀释浓度下，ＭＣＹＳ