Excretion pattern investigation of urinary normal and modified nucleosides of breast cancer patients by RP-HPLC and factor analysis method

Modified nucleosides, formed post-transcriptionally in RNA by a number of modification enzymes, are excreted in abnormal levels in the urine of patients with malignant tumors. To test their usefulness as tumor markers, and to compare them with the conventional tumor markers, a reversed-phase high-performance liquid chromatographic (RP-HPLC) method and a factor analysis method have been used to study the excretion pattern of nucleosides of breast cancer patients. A clear cut differentiation of the breast cancer group and the healthy individuals in two clusters without overlapping was obtained. Copyright (C) 2000 John Wiley & Sons, Ltd.

Factor analysis used to peak position optimization and spectral interference correction in inductively coupled plasma atomic emission spectrometry

Target transformation factor analysis was used to correct spectral interference in inductively coupled plasma atomic emission spectrometry (ICP-BES) for the determination of rare earth impurities in high purity thulium oxide. Data matrix was constructed with pure and mixture vectors and background vector. A method based on an error evaluation function was proposed to optimize the peak position, so the influence of the peak position shift in spectral scans on the determination was eliminated or reduced. Satisfactory results were obtained using factor analysis and the proposed peak position optimization method.

APPLICATION OF FACTOR-ANALYSIS TO CORRECTION OF SPECTRAL OVERLAP INTERFERENCE IN INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION-SPECTROMETRY

Correction of spectral overlap interference in inductively coupled plasma atomic emission spectrometry by factor analysis is attempted. For the spectral overlap of two known lines, a data matrix can be composed from one or two pure spectra and a spectrum of the mixture. The data matrix is decomposed into a spectra matrix and a concentration matrix by target transformation factor analysis. The component concentration of interest in a binary mixture is obtained from the concentration matrix and interference from the other component is eliminated. This method is applied to correcting spectral interference of yttrium on the determination of copper and aluminium: satisfactory results are obtained. This method may also be applied to correcting spectral overlap interference for more than two lines. Like other methods of correcting spectral interferences, factor analysis can only be used for additive spectral overlap. Results obtained from measurements on copper/yttrium mixtures with different white noise added show that random errors in measurement data do not significantly affect the results of the correction method.

FACTOR-ANALYSIS SPECTROPHOTOMETRY FOR THE SIMULTANEOUS DETERMINATION OF VALENCE STATES OF METALS

Two M(n+)-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol systems for the simultaneous determination of the valence states of Cr and Fe using factor analysis were studied. (1) At pH 4.0, Cr(III) and Cr(VI) react with the reagent to form stable complexes and a slight difference in the wavelengths of maximum absorption (lambda(max.)) between the two complexes is observed when the sodium lauryl sulfate, which also acts as a solubilizing and sensitizing agent, is added, viz., 590 nm for Cr(III) and 593 nm for Cr(VI) complexes. (2) In the presence of ethanol, both Fe(II) and Fe(III) form 1:2 complexes with the reagent at pH 2.5-3.5 and the lambda(max.) of the Fe(II) and Fe(III) complexes is at 557 and 592 nm, respectively. In the target transformation factor analysis, the K coefficients calculated from the standard mixtures by classical least-squares analysis and a non-zero intercept added to each wavelength are used as the target vector instead of the pure component standards; this can decrease the analysis errors introduced by the interaction between the two species and by deviations from Beer's law.

FACTOR-ANALYSIS APPLIED TO X-RAY-DIFFRACTION DIAGRAMS OF POLYMER BLENDS

The feasibility of applying the method of factor analysis to X-ray diffraction diagrams of binary blends of polypropylene and ethylene-propylene-diene terpolymer (PP/EPDM) was examined. The result of mathematical treatment was satisfactory. The number of scattering species and their concentrations in six kinds of PP/EPDM blends were determined. The separation of the spectral peaks of each species in the blends, contributing spectral intensities, was carried out.

色散管理孤子传输特性分析

色散管理孤子光波系统的色散图周期由具有正负色散值的两段光纤组成，并且在系统中周期性放置集中放大器及滤波器装置。采用变分法获得了描述此种系统性能的常微分方程组，并利用这组方程数值研究了各种参量对孤子传输性能的影响。研究结果表明，虽然从整体趋势上看，当要求色散管理孤子有较好的稳定性时，应适当降低系统的色散管理强度，但在一定条件下，调整光纤放大器的位置以及正负色散光纤的色散差值，可同时获得相对较大的色散管理强度和好的孤子稳定性；总体来讲，集总放大器的位置会对孤子传输的稳定性、色散管理孤子的能量增强因子、脉冲最佳

花青素代谢途径上结构基因和调控基因的多态性分析

在生物个体中，很多基因位点都存在多个等位基因。这些等位基因存在的生物学意义是什么？是中性突变的结果还是进化选择的结果？本文以花青素代谢途径为平台，以圆叶牵牛为研究对象，试图探讨相关的生物学问题。具体问题是：（1）等位基因的多样性在调控基因和结构基因中是否有所不同？（2）两个等位基因的启动区会有什么不同？ 我们首先在来自新圭亚那、美国和中国的17个圆叶牵牛个体中调查了花青素代谢途径上第一个关键酶基因CHS-D基因和下游的最后一个基因UF3GT，以及三类调控基因MYB1、bHLH1、WDR1的多态性。目前的研究结果表明，CHS-D位点有三个等位基因，其中在中国的西南部地区发现了一个新的等位基因CHS-D-SINO1。下游的结构酶基因UF3GT位点存在3个等位基因，其中的UF3GT-c是我们在新圭亚那的三个个体中所新发现的。调控基因中，MYB1位点存在两个等位基因，其中一个是新发现的。bHLH2位点存在3个等位基因，均为新发现的。WDR1位点也存在三个等位基因，其中两个都是在本实验中新得到的。调控基因和结构基因位点的等位基因数量没有明显差别。 为了进一步了解等位基因的差异，我通过inverse PCR的方法得到了花青素代谢途径上F3H基因的两个等位基因F3H1和F3H3的启动区序列。通过分析发现，F3H1和F3H3的编码区序列相似性达到99%，而启动区序列相似性为79.7%，总体相似性87.1%。可见，这两个等位基因的差异主要集中在启动区。 F3H1启动区包含了16个bHLH蛋白的识别位点；而F3H3启动区包含9个bHLH蛋白的识别位点。其中，两者完全一样的有7处。除bHLH蛋白以外，F3H1和F3H3都分别含有一个MYB蛋白的识别位点，但两者所处的位置不同。这些数据表明这两个等位基因在功能上应该差别不大，但在应答调控基因对其的调节作用上可能有所差别。

鄂尔多斯高原景观要素空间格局及植物与环境关系的定量解析

鄂尔多斯高原是一个多层次、复杂的生态过渡带，具有复杂多样的环境条件、生态特点，因此也就具有复杂多样的植物与环境关系。本文从群落和景观两个尺度水平上研究鄂尔多斯高原植物或植被与环境关系及景观空间格局。利用鄂尔多斯高原野外植物群落样方调坦数据、微生境环境数据、气候数据，以典范对应分析（Canonical Correspondence Analysis, CCA）的方法分析了鄂尔多斯高原植物分布空间格局与环境要素的关系并对秋类环境要素对鄂尔多斯高原植物空间分布格局的贡献进行了定量分解;利用1:500 000 鄂尔多斯高原植被、土壤、土地利用、土地沙漠化类型等专题地图在GIS支持下分析了鄂尔多斯高原景观空间格局、并利用上述专题图数据加上鄂尔多斯高原气候数据库分析了土壤、土地利用、土地利用、土地沙漠化和气候等对鄂尔多斯高原植被空间分布格局的作用。通过分析，得到了以下主要结论： 1 在分析方法上、利用典范对应分析的方法，把植物分布的空间因素与环境因素分离的方法发展为植物分布空间格局不同类型影响因素作用的定量分离，提出了相应的概念模型和实现方法。 2 影响鄂尔多斯高原植物分布空间格局的主要微生境环境要素是基质类型、地下水位、覆沙厚度等，而影响鄂尔多斯高原分布空间格局的主要气候要素中，降水和干湿指标的作用大于温度和热量指标的作用。 3 通过对鄂尔多斯高原植物分布空间格局与环境关系的研究，以植物对微生境环境要素的反应为根据，把鄂尔多斯高原主要植物划分为4个大类群：梁地植物、沙地植物、草甸植物和耐盐植物。根据它们对气候要素的反应，把鄂尔多斯高原主要植物划分为典型草原植物、荒漠草原植物和草原化荒漠植物3大类。进一步，根据鄂尔多劳动保护高原植物与环境关系的研究，进行了鄂尔多斯高原植被功能型划分的尝试，得到了鄂尔多斯高原的12种主要植被功能型。 4 对鄂尔多斯高原植物分布的空间格局的影响环境因素的贡献作了定量地分解。分析结果显示：鄂尔多斯高原植物分布空间格局中有27.02％可由已知环境变量得到解释，其中21.56％与微生境环境要素相关，7.51％与气候要素的作用有关，而气候与微生境环境要素的耦合作用的份额为2.05％。根据植物生长是否直接受到地下水的影响，鄂尔多斯高原存在两大类型生态特点差异明显的生境类型：中性立地和隐域生境，对两大类型生境上影响植物空间分布格局的环境要素的作用也进行了定量分解。分析结果表明：对于植物生长不直接受地下水影响的中性立地，已知环境要素的作用可以解释植物空间分布格局总信息的29.36％，稍大于对总体上鄂尔多斯高原植物分布空间格局的解释，其中9.23％与气候要素相关，22.08％与微生境环境要素相关，而两种类型环境要素的耦合作用则占1.95％。对于植物生长直接受到地下水影响的隐域生境，所有已知环境要素对植物分布空间格局的贡献率为72.28％，其中气候要素的作用为30.31％，微生境环境要素的作用为49.08％，两类环境要素的耦合作用为7.11％。 5 描述景观空间格局的指数多种多样，这些能数在描述特定区域的景观空间格局时是有信息冗余的。本文对利用FRAGSTATS所获得的鄂尔多斯高原植被、土壤、土地利用、土地沙漠化等景观分量的20个景观指数实施了因子分析。通过因子分析，我们可以把描述鄂尔多斯高原景观空间格局的景观指数归并为以下8类：多样性指数、斑块多度指数、斑块类型丰富度指数、斑块面积指数、斑块形状指数、分形维数、空间配置指数和斑块面积变异指数。通过因子分析，还得到了这些景观指数对描述鄂尔多斯景观格局的共性特征：在描述鄂尔多斯高原景观空间格局时，作用最大的是多样性指数、斑块多度指数、面积加权平均斑块形状指数和面积加权平均分形维数，其次是斑块类型丰富度指数、平均分维指数、平均形状指数和斑块面积指数，而空间配置指数（扩散与毗连指数）和斑块面积变异指数的作用则比较微弱。 6 对鄂尔多斯高原景观指数的因子分析是非常有效和成功的。因子分析对鄂尔多斯高原植被、土壤、土地利用、土地沙漠化景观指数的分析分别得到了5-6个主要因子，可以表达原有20个景观指数所表达信息的91.1-96.0％，即可以反应鄂尔多斯高原景观空间格局的大部分信息。本文所进行的因子分析对因子进行了方差最大化(Varimax)正交旋转的处理，因子分析得到的每一个主要因子都有一个或几个与之相关性非常高的景观指数与之对应，因此，就可以用与因子分析所得主要因子相关性最高的景观指数代替该主要因子来表达鄂尔多斯高原的景观空间格局。另外还因为有些景观指数之间具有极高的相关系数，所以对因子分析所得到的景观指数可以进一步精减，最后利用因子分析成功地把原有20个景观指数减少到了11个。最后被选来描述鄂尔多斯高原景观格局的景观指数有下列11个：MSIEI（修正的Simpson均匀度指数）、AWMPFD（面积加权平均斑块分形维数）、AWMSI（面积加权平均形状指数）、NP（斑块数目）、PR（斑块类型丰富度）、MSI（平均形状指数）、MPFD（平均斑块分形维数）、MPS（平均斑块面积）、PSCV（斑块面积变异系数）、DLFD（双对数分形维数）和IJI（扩散与毗连指数）。 7 鄂尔多斯高原植被、土壤、土地利用在景观组成结构上具有一个共同特点，就是各种类型的面积差异极大，少数类型占有极大比重，而其余面积则很小。产生这一情形的原因主要与人为活动的强烈影响有关，表现在地带性的植被与土壤面积所占的比重不高，沙地、沙生植被与风沙土则占有很大比重。 8 以地带性植被和滩地隐域性植被表示的鄂尔多斯高原的原生植被仅占高原面积的不足30％，而以地带性土壤和滩地隐域性土壤表示的原生性土壤占鄂尔多斯高原总面积的近40％，说明土壤退化不如植被退化严重，或滞后于植被退化。 9 鄂尔多斯高原各景观指数的空间变化曲线，植被与土壤很相近，具有非常相似的格局;土地利用景观格局空间变化特征与植被、土壤等明显不同;土地沙漠化的景观格局空间变化曲线介植被曲线、土壤曲线与土地利用曲线之间，说明土地沙漠化不仅是一个受人为活动影响的过程，而且与自然过程密切相关。 10 鄂尔多斯高原景观格局的空间梯度变化表现出了东西向和南北向的梯度，但总体上以东西向的变化比较明显。 11 通过鄂尔多斯高原土壤类型、土地利用、土地沙漠化等景观要素、气候、空间要素与鄂尔多斯高原植被空间分布格局的CCA分析，探讨了它们之间的相互关系。以对鄂尔多斯高原植被组成数据的总方差解释的百分率为标准，土壤对鄂尔多斯植被分布的空间格局的作用最大，其方差贡献率可达44.28％，其次是土地利用与鄂尔多斯高原植被的关系也很密切，土地利用对鄂尔多斯高原植被空间分布格局的方差贡献率为22.45％，空间因素对鄂尔多斯高原植被空间分布格局的贡献率为17.51％，土地沙漠化对鄂尔多斯高原植被空间分布格局的贡献为15.65％，排在第四位;气候因素对鄂尔多斯高原植被空间格局的贡献率为11.95％，居第五位。 12 在气候要素对鄂尔多斯高原植被空间分布格局的作用中，降水与干湿指标的作用大于温度与热量指标的作用。这一点与利用野外调查样方的群落数据植物与气候关系的分析是完全一致的。CCA分析还表明鄂尔多斯高原植被空间格局的东西向变化大于南北向分异。 13 在群落和景观水平上，鄂尔多斯高原植物空间分布或植被格局的影响因素的作用具有相似的格局，即气候因子的作用明显地小于地质、土壤、水文等微生境环境要素（群落水平）或土壤（景观水平）的作用，并在这两个尺度上气候要素对植物空间分布或植被格局的定量解释份额上也是非常相近的，都仅有10％左右。气候因子对鄂尔多斯高原植物空间分布格局的这种弱的解释能力，从侧面说明了人为活动等非自然因素对鄂尔多斯高原植物空间分布格局的强烈作用。 14 在鄂尔多斯高原生态系统管理上，应协调人与自然的关系;加强鄂尔多斯高原的生物多样性保育，对于本区生态和经济对非常重要的滩地，应协调好对其开发利用与保护的关系;在鄂尔多斯高原土地沙漠化防治方面，应把调整人地关系与自然生态背景与条件相结合，如使用“三圈”模式等生态系统管理模式等。

Phytoplankton biomass in relation to nutrients and zooplankton in thirty subtropical lakes adjacent to the Yangtze River, China

A total of 30 shallow lakes, located along the middle and lower reaches of the Yangtze River, were studied to assess the relative importance of nutrients and zooplankton biomass in determining the phytoplankton biomass in subtropical China. Zooplankton biomass and nutrients both varied greatly in these lakes. Factor analysis and multiple linear regression showed that phytoplankton biomass was positively correlated with TN, NH4+, NO3- and TP, while it did not show any negative relationship to zooplankton biomass. Meanwhile, the phytoplankton biomass showed contrary relationships to the mass ratio of TN/TP in spring and summer, suggesting that in nutrient-richer lakes the dominant phytoplankton species have different preferences for TN/TP ratio. The insignificant top-down control of phytoplankton biomass may be attributed to the dominance of small-sized crustaceans and low crustacean biomass resulting from cyanobacterial dominance and planktivorous fish predation as well as other factors. Thus, it is likely that nutrients were more important than zooplankton biomass in explaining the total variance of phytoplaDkton biomass in these subtropical lakes.

Study of normal and modified nucleosides in serum by RP-HPLC

Modified nucleosides derived predominantly from transfer ribonucleic acid (tRNA) have been studied as possible tumor markers. In this paper a reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been applied to study 15 normal and modified nucleosides in serum. The nucleoside levels in normal human serum were established, and the concentrations of 15 nucleosides in serum from 19 cancer patients were determined, it was found that the concentrations of modified nucleosides were significantly higher in patient sera. Based on 15 nucleoside concentrations in serum, factor analysis could classify correctly 90% of cancer patients from the normal humans Further work showed that urine was slightly better than serum when determining nucleosides as biological marker candidates. More work is ongoing to determine the clinical usefulness of modified nucleosides as tumor markers.

The relation between formal potential and structure of indophenols and molecular mechnism of electrooxidation

The molecular structural parameters of indophenol and its derivatives were calculated by semi-empirical molecular orbital quantum chemical method,The relation between molecular structural parameters and formal potentials was analyzed by principal factor analysis and multiple Linear regression method. It was found that the formal potential of indophenols has a good relation with two-center electron exchange energy, E-ex (2), resonance energy of O-C bond, E-ex (C-1-O), and molecular ionization potential, I-p, among 19 moleclular structural parameters. The regression equation is E-0' = 1. 47 x 10 (-3) E-ex (two) - 5. 74 x 10 (-2) E-ex (C-1 - O) - 1. 41 x 10 (-2) I-p with RC = 0. 9999 and SD = 0. 00424. It was confirmed by the relation between structure parameters and formal potentials, and the thermodynamic stability of its intermediate products that the H (+) ionization is prior to the electron transfer step in the oxidation mechanism.

Information system for ICP-AES in Windows environment

An information system for inductively coupled plasma atomic emission spectrometry (TCP-BES) in MS Windows environment was developed based on the previous work in the laboratory. The system contains the data of about 28 000 spectral lines and a function of ICP spectral simulation,so it would be very helpful for line selection. The system also contains the Kalman filter and factor analysis programmes written with MS Visual Basic(version 4.0), which can be used for spectral interference correction and peak position optimization. A large amount of real spectral scanning data of rare earth elements were included in the system for user's references. All these characteristics made the system more useful and practical.

北黄海表层沉积物粒度分布特征及其对沉积环境的指示

本文基于北黄海1140个表层沉积物样品的粒度分析结果，来探讨沉积物粒度组成、分布特征与物源和水动力环境的关系。结果表明，研究区底质类型主要有泥、粉砂、砂质粉砂、粉砂质砂、砂五种类型，少数站位含有砾石。砾石则主要分布在长山列岛附近海域、大连湾口及其东南近海；砂主要分布在123.3°E以东的海域和长山列岛附近海域；粉砂主要分布在研究区的西南部和大连湾外海；粘土的含量较低，含量大于30%的区域仅分布在研究区的西南部，大于16%的区域有向北和东北延伸的趋势，在大连湾至广鹿岛近海沉积物粘土含量也大于16%。 根据对沉积物粒度各粒级的因子分析结果,结合沉积物的Folk分类和Pejrup分类,将北黄海划分成五个主要沉积环境分区，分别受山东半岛沿岸流、黄海暖流、强潮流场、长山列岛和辽东沿岸流控制。北黄海中西部沉积物主要受山东半岛沿岸流影响，经过本区的黄海暖流限制了山东半岛沿岸流携带的细粒物质向东和向北的扩散和运移，研究区东部沉积作用的主控因素是潮流，长山列岛中部海域受附近岛屿剥蚀物影响，大连湾外海受辽东半岛沿岸流控制。研究区西南部细粒沉积物的粒度分布具有不均匀性和南北不对称性，其形成主要受控于山东半岛沿岸流和黄海暖流及其相互作用，潮流可能也起一定作用。