26 resultados para EEG, fMRI, sinestesia
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
Although studies on placebo effect proved the placebo expectation established by pain-alleviating treatment could significantly alleviate later pain perception, or the placebo expectation established by anxiety-reducing treatment could significantly reduce the intensity of induced negative feelings, it is still unclear whether or not the placebo effect can occur in a transferable manner. That is, we still don’t know if the placebo expectation derived from pain-alleviating can significantly reduce later negative emotional arousal or not. Experiment 1: We compared the effect of the verbal expectation (purely verbal induction and without pain-alleviating reinforcement) with the reinforced expectation (building the belief in the placebo’s ataractic efficiency on unpleasant picture processing by secret reduction of the intensity of the pain-evoking stimulus) on the negative emotion. The results showed that the expectation, which was reinforced by actual analgesia, was transferable and could produce significant placebo effect on negative emotional arousal. However, the expectation that was merely induced by verbal instruction did not have such power. Experiment 2 both examined the direct analgesic effect of the placebo on the sensory pain (how strong is the pain stimulus) and emotional pain (how disturbing is the pain stimulus) and the transferable ataractic effect of the placebo on the negative emotion (how disturbing is the emotional picture stimulus), and further proved that the placebo expectation that was established from pain-reducing reinforcement not only induced significant placebo effect on pain, but also significant placebo effect on unpleasant feeling. These results support the viewpoint that the reduction of affective pain based on the conditioning mechanism plays an important role in the placebo analgesia, but can’t explain the transferred placebo effect on visual unpleasantness. Experiment 3 continued to use the paradigm of the reinforced expectation group and recorded the EEG activities, the data showed that the transferable placebo treatment was accompanied with decreased P2 amplitude and increased N2 distributed, and significant differences between the transferable placebo condition and the control condition (i.e., P2 and N2) were observed within the first 150-300 ms, a duration brief enough to rule out the possibility that differences between the two conditions merely reflect a bias “to try to please the investigator. In Experiment 4, we selected the placebo responders in the pre-experiment and let them to go through the formal fMRI scan. The results found that the transferable placebo treatment reduced the negative emotional response, emotion-responsive regions such as the amygdala, insula, anterior cingulate cortex and the thalamus showed an attenuated activation. And in the placebo condition, there was an enhanced activation in the subcollosal gyrus, which may be involved in emotional regulation. In conclusion, the transferable placebo treatment induced the reliable placebo effect on the behavior, EEG activity and bold signal, and we attempted to discuss the pychophysiological mechanism based on the positive expectancy.
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眶额叶皮质与中脑边缘多巴胺奖赏系统有着复杂的相互纤维联系.先前的研究探讨了药物成瘾过程中眶额叶皮质的脑电活动.在本实验中,将探讨食物奖赏和渴求过程中该皮质的脑电活动.实验采用了两个环境:对照环境和食物刺激相关的环境.首先,训练大鼠在食物刺激相关的环境中吃巧克力花生豆,而后在该环境中设置两种不同的刺激方式:能看到和闻到但不能吃到(渴求实验),或者仍旧可以吃到巧克力花生豆(奖赏实验):同时进行左侧眶额叶皮质的脑电记录.结果发现,在食物刺激相关的环境中大鼠Delta频段(2-4Hz)的脑电活动与食物刺激显著相关,此外,与在对照环境中相比,其相对功率在食物渴求时下降而在食物奖赏时升高.本实验表明,食物相关的奖励可以改变大鼠眶额叶皮质的脑电活动,而且,Delta频段的脑电活动能够作为监测该奖励的一个指标.
Resumo:
Investigating the activities of the prefrontal cortex (PFC) in the process of addiction is valuable for understanding the neural mechanism underlying the impairments of the PFC after drug abuse. However, limited data are obtained from primate animals and few studies analyze Electroencephalogram (EEG) in the gamma band, which plays an important role in cognitive functions. In addition, it is yet unclear whether drug abuse affects the orbitofrontal cortex (OFC) and dorsolateral PFC (DLPFC) - the two most important subregions of the PFC - in similar ways or not. The aim of this study is to address these issues. We recorded EEG in the OFC and DLPFC in three rhesus monkeys. All animals received a course of saline (NaCl 0.9%, 2 ml) injection (5 days) followed by 10 days of morphine injection (every 12 h), and then a further series of saline injection (7 days). A main finding in the present study was that morphine decreased EEG power in all frequency bands in a short period after injection in both the OFC and DLPFC in monkeys. And gamma power decreased not just in short period after morphine injection but lasted to 12 h after injection. Moreover, we found that although the changes in EEG activities in the OFC and DLPFC at 30-35 min after injection were similar, the DLPFC was more sensitive to the effect of morphine than the OFC. (c) 2005 Elsevier B.V. All rights reserved.
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Background and objectives: Pentobarbital and ketamine are commonly used in animal experiments, including studies on the effects of ageing on the central nervous system. The electroencephalogram is a sensitive measure of brain activity. The present study i
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A monolithically integrated CMOS bioamplifier is presented in this paper for EEG recording applications. The capacitive-coupled circuit input structure is utilized to eliminate the large and random DC offsets existing in the electrode-tissue interface. Diode-connected NMOS transistors with negative voltage between gate and source are candidates for large resistors necessary to the bioamplifier. A passive BEF (Band Eliminator Filter) can reduce 50 Hz noise disturbance strength by more than 60 dB. A novel analysis approach is given to help determine the noise power spectral density. Simulation results show that the two-stage CMOS bioamplifier in a closed-loop capacitive feedback configuration,provides an AC in-band gain of 39.6 dB, a DC gain of zero, and an input-referred noise of 87 nVrms integrated from 0.01 Hz to 100 Hz.
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针对用于服务机器人的脑机接口系统中脑电信号模式识别精度不高,不能满足机器人多任务要求的问题,提出一种基于C-支持向量多分类机的多类复杂手操作EEG信号模式识别方法,并将其应用到复杂手操作的EEG信号模式识别试验中,实现一个4类复杂手操作的模式识别,实验结果表明,与之前用BP神经网络进行识别相比,识别率由85%提高到了90%。
Resumo:
In the present study, based on processing efficiency theory, we used the event-related potentials (ERP) and functional magnetic resonance image (fMRI) techniques to explore the underlying neutral mechanism of influences of negative emotion on three subsystems of working memory, phonological loop、 visuospatial sketh pad and the central executive. The modified DSMT (delayed matching-to-sample task) and n-back tasks were adopted and IAPS (International Affective Picture System) pictures were employed to induce the expected emotional state of subjects. The main results and conclusions obtained in the series of experiments are as the following: 1. In DSM tasks, we found P200 and P300 were reduced by negative emotion in both spatial and verbal tasks, however the increased negative slow wave were only observed in spatial tasks, not in verbal tasks. 2. In n-back tasks, the updating function of WM associated P300 was affected by negative emotion only in spatial tasks, not in verbal tasks. Current density analysis revealed strong current density in the fronto-parietal cortex only in the spatial tasks as well. 3. We adopted fMRI-block design and ROIs analysis, and found significant emotion and task effects in spatial WM-associated right superior parietal cortex; only emotion effect in verbal WM-associated Broca’s area; the interaction effect in attention-associated medial prefrontal area and bilateral inferior parietal cortex. These results implied the negative emotion mainly disturbed the spatial WM-related areas, and the attention control system play a key role in the interaction of spatial WM and negative emotion. 4. to further examine the effects of positive、negative and neutral emotion on tasks with different cognitive loads, the selective effect of emotion on the ERP components of spatial WM was only found in 2-back tasks, not in visual searching tasks. So, firstly the positive emotion as well as negative emotion selectively disturbed on spatial WM in light of the attention resource competition mechanism. Secondly, the selective influences based on the different WM systems, not the properties of spatial and verbal information. At last, the manner of the interaction of emotion and cognition is correlated with the cognitive load.
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Bats (Chiroptera) are the second-most abundant mammalian order in the world, occupying a diverse range of habitats and exhibiting many different life history traits. In order to contribute to this highly underrepresented group we describe the sleep architecture of two species of frugivorous bat, the greater short-nosed fruit bat (Cynopterus sphinx) and the lesser dawn fruit bat (Eonycteris spelaea). Electroencephalogram (EEG) and electromyogram (EMG) data were recorded from multiple individuals (>= 5) by telemetry over a 72-h period in a laboratory setting with light/dark cycles equivalent to those found in the wild. Our results show that over a 24-h period both species spent more time asleep than awake (mean 15 h), less than previous reported for Chiroptera (20 h). C sphinx spent significantly more of its non-rapid eye movement sleep (NREM) and rapid eye movement sleep (REM) quotas during the light phase, while E. spelaea divided its sleep-wake architecture equally between both light and dark phases. Comparing the sleep patterns of the two species found that C. sphinx had significantly fewer NREM and REM episodes than E. spelaea but each episode lasted for a significantly longer period of time. Potential hypotheses to explain the differences in the sleep architecture of C. sphinx with E. spelaea, including risk of predation and social interaction are discussed. (C) 2010 Published by Elsevier B.V.
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下载PDF阅读器目的 利用功能磁共振(fMRI)和局部一致性(regional homogeneity,ReHo)探讨抑郁症首次发病(以下简称首发)患者在静息态脑功能是否存在异常及异常部位.方法 对34例符合美国精神疾病诊断与统计手册第4版诊断标准的首发抑郁症患者(抑郁症组)和34名性别、年龄、文化程度匹配的健康志愿者(对照组)进行静息态fMRI扫描.结果 抑郁症组静息态脑血氧水平依赖信号的ReHo高于对照组的脑区有左侧额叶眶回、顶下小叶、颞上回,右侧额内侧回、顶下小叶、小脑后叶;低于对照组的脑区有左颞下回、右颞上同和胼胝体、双侧后扣带回(P<0.005,K≥10).结论首发抑郁症患者在静息态存在多个腩区功能活动的异常,并可能和抑郁症的病理机制有关.
Resumo:
The research of dipole source localization has great significance in both clinical research and applications. For example, the EEG recording from the scalp is widely used for the localization of sources of electrical activity in the brain. This paper presents a closed formula that describes the electric field of dipoles at arbitrary position, which is a linear transformer called the transfer matrix. The expression of transfer matrix and its many useful characteristics are given, which can be used for the analysis of the electrical fields of dipoles. This paper also presents the closed formula for determining the location and magnitude of single dipole or multi-dipoles according to its electrical field distribution. A calculation result for a single dipole shows that the dipole will be located at the midpoint of a line segment if there are equivalent fields at its two ends.
Resumo:
已有的研究表明,眶额叶在解剖上与现在已知的药物滥用相关的脑区是紧密联系在一起的。例如,眶额叶在药物滥用和强迫性重复行为中起作用,且随着脑成像技术的应用,越来越多的证据表明眶额叶参与了药物滥用。但是我们并不了解在阿片给药和戒断期间眶额叶脑区活动是如何变化的。因此,我们在实验中采用了Mn2+增强的核磁共振成像(Manganese-enhanced magnetic resonance imaging,MEMRI,4.7T)技术和脑电(EEG)记录的方法,以研究大鼠眶额叶在给与阿片类药物(盐酸吗啡)以及戒断过程中的动态变化。 MEMRI是一种近年才发展起来的新型技术。研究表明,Mn2+是Ca2+的类似物,可以通过Ca2+通道进入兴奋性的神经元里面并结合到胞内的蛋白质和核酸上的Ca2+和Mg2+结合位点上 (MILDVAN and COHN, 1963; EISINGER et al., 1965)。另外,Mn2+的顺磁性也为它成为核磁共振成像的造影剂提供了前提条件。可是成功应用MEMRI的前提就是要在适当的时间把合适剂量的Mn2+传递到靶点上。因此,Mn2+在注射到靶点后,是否能够在有效的时间内反映大脑活动的变化就成为一个非常重要并且在技术上较为棘手的问题。在给实验大鼠脑区微量注射Mn2+(80mMol/L,200nl)的同时,通过微量注射兴奋性神经递质谷氨酸(Glu 0.5mM/L)或抑制性递质γ-aminobutyric acid(GABA 0.5M/L)以改变靶点神经元兴奋性的方法,检测Mn2+能否反映脑区的活动变化。另外,我们随机选取实验动物,分别在注射Mn2+ 3小时、5小时和8小时后对三组大鼠(n=5)进行10%福尔马林灌流,并且通过观察大鼠眶额叶脑区Mn2+强度的变化来研究最佳的灌流时间。我们的实验结果表明,Mn2++Glu组的右侧脑区/左侧脑区的Mn2+亮度比Mn2+空白对照组增加了20%(p=0.016, student t-test, *p <0.05),也远大于Mn2++GABA组(p=0.047, *p<0.05)。结果表明,当神经元被兴奋的时候,较多的Mn2+可以通过Ca2+通道进入兴奋的神经元内,使得Mn2+的成像亮度增加。由于Mn2+成像亮度的增加可以反映神经元的兴奋活动,因此可以显示出靶点区的脑活动。另外,在研究灌流时间对Mn2+亮度影响的实验中发现,注射Mn2+ 5小时后灌流得到的信噪比分别比注射Mn2+3小时(p=0.055)和8小时(p=0.004,*p<0.05)高出24%和32%。总之,我们采用微量注射Mn2+(80mM/L,200nl)后5小时用10%福尔马林心脏灌流的方法获得了较好的结果。另外在试验中我们首先观测了大鼠吗啡戒断后的行为学指标和检测大鼠戒断后条件化位置偏好的程度。实验结果表明大鼠可以建立非常明显的条件化位置偏好,但在湿狗抖等行为学指标上无明显症状。这说明大鼠对于吗啡(10mg/kg, 一天两次,持续12天)形成了明显的心理依赖而无明显的生理依赖。此外,MEMRI的结果表明,在吗啡给药的第1天和第6天,大鼠眶额叶的Mn2+强度与空白对照组相比有显著的降低( one-way ANOVA, Post Hoc Dunnett’s C Tests), F (6,28)=7.242, P<0.001);而在戒断第3天又恢复到正常水平,在戒断第5天和第7天Mn2+强度跟空白对照组相比没有显著性差别(one-way ANOVA, *p<0.05)。脑电(EEG)的结果表明,急性吗啡诱导的gamma波段的EEG显著降低(Two-way ANOVA, F(1,10)=13.626,p=0.006)。然而在戒断第1天gamma波段的EEG与空白对照组相比是增加的。在戒断第3天和戒断第5天,gamma波段的EEG与空白对照组相比也有显著性增强。以上研究结果表明:大鼠眶额叶脑区的动态变化与整个吗啡给药和戒断过程是密切相关的;此外,MEMRI在探讨药物滥用以及成瘾等机制上有很大的应用前景。
Resumo:
长期以来,星形胶质细胞(astrocyte,AS)被认为具有细胞隔离、支持、保护和营养神经元的作用。近来,人们逐渐发现,AS还有许多其他重要的功能。例如,谷氨酸是中枢神经系统中重要的兴奋性神经递质,AS摄取和合成谷氨酸的作用可能影响学习、神经发育及发情周期等多种脑功能,并影响突触形成和突触传递效能。有研究发现,成年海马齿状回颗粒下层具有神经干细胞潜能的AS,可以分化成新生神经元。此外,海马成熟的AS还参与调节神经发生,决定神经干细胞的分化方向。大鼠海马新生神经元与海马依赖性记忆(happocampal- dependent memory)密切相关。提示,AS与学习记忆密切相关。但迄今为止,尚未见AS参与工作记忆的报道。本研究以特异性抑制AS细胞的化合物fluorocitrate(FC)作为工具药来探讨AS在大鼠工作记忆中的作用及作用机制。FC可抑制AS三羧酸循环中乌头酸酶(aconitase)的活性,从而抑制AS摄取谷氨酸的功能,其作用是可逆的,目前已经被广泛用于研究AS的功能。我们通过慢性导管在大鼠双侧前额叶(prefrontal cortex,PFC),双侧海马(hippocampus,HIP)和单侧侧脑室(lateral ventricle,LV)分别注射FC,记录注射后15-75min内自由活动大鼠的脑电图(EEG)功率变化,以判断FC对大鼠PFC和HIP神经元兴奋性的影响,并在注射后15-75min内完成T-迷宫空间交互延缓(spatial delayed alternation task)反应测试,分析动物的空间工作记忆功能。结果发现在双侧PFC和双侧HIP分别注射1,2,5nM FC (0.5μl),仅2,5nM显著增加HIP EEG的功率( p < 0.05 vs. SAL),但对交互延缓反应成绩无明显影响,所有剂量对PFC的EEG功率没有显著影响,且不影响动物操作交互延缓反应作业的成绩(ALL p > 0.1 vs. SAL)。在单侧LV注射0.05,0.5nM FC (10μl)后,PFC的EEG功率没有明显变化,而HIP的EEG功率有增加。该剂量的FC对大鼠操作空间交互延缓作业的成绩无明显影响。在单侧LV注射2nM FC(10μl)同时显著增加PFC和HIP的EEG功率,并显著提高大鼠操作空间交互延缓反应作业中的正确率,改善大鼠的工作记忆( p < 0.05 vs. SAL)。单侧LV注射5nM FC(10μl)同时显著降低PFC和HIP的EEG功率(ALL p < 0.05 vs. SAL)和大鼠操作作业的正确率,损伤大鼠的工作记忆( p < 0.05 vs. SAL)。表明只有在PFC和HIP的EEG功率同时受到影响的时候,大鼠操作作业的正确率才会发生显著改变。已有研究指出,前额叶和海马均参与工作记忆调控。在LV给FC将同时作用于双侧PFC和双侧HIP。因此,我们认为,FC对大鼠空间交互延缓作业成绩的影响可能是通过抑制PFC和HIP的AS功能产生的。在单侧LV注射低浓度(0.05,0.5nM)的FC对大鼠空间交互延缓作业成绩和EEG功率水平均无明显影响,可能与脑内AS的功能尚可,谷氨酸水平升高不明显有关,而高浓度(5nM)FC可能较强地抑制AS摄取胞外谷氨酸的功能,胞外谷氨酸水平过度升高,损伤神经元的活性,使EEG功率降低,结果导致工作记忆受损。高浓度FC也可能直接损伤神经元的活性。本研究结果表明,FC短期抑制PFC和HIP的AS功能,将使PFC和HIP胞外谷氨酸水平升高,从而改变PFC和HIP神经元的兴奋性,使大鼠的空间工作记忆功能发生改变。揭示了工作记忆不仅依赖于PFC-HIP回路中神经元的正常功能,也与PFC-HIP回路中AS的功能正常与否密切相关。
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1 初级视觉皮层功能,GABA系统功能在衰老过程中的变化 本章首先对衰老过程中神经形态学和神经电生理学上的研究进行了综述,然后报道了作者的博士学位论文研究工作。实验采用神经电生理的手段,探讨初级视觉皮层(primary visual cortex;V1)功能,以及GABA(gamma-aminobutyric acid)系统功能在衰老过程中的变化。 实验1和2均采用单细胞记录技术,检测了中年猴V1细胞的方位选择性、方向选择性、自发放和最大反应,并与年轻和老年猴进行对比;比较了年轻和老年猴V1细胞的感受野外周抑制能力。在实验3中,我们记录了年轻和中年大鼠在给予GABA直接或间接的激动剂,戊巴比妥钠或氯胺酮{通过拮抗NMDA(N-methyl-D-aspartate)受体}后,其皮层的EEG(electroencephalogram)活动,并分析与年龄相关的差异。结果如下: 实验1:中年猴V1细胞的方向选择性和自发放介于年轻猴和老年猴之间,而方位选择性和最大反应与年龄之间没有相关性。 实验2:感受野外周区的最优刺激明显降低了年轻和老年猴具有高方位选择性细胞的比例。同时,年轻猴所有细胞,以及老年猴高方位选择性细胞具有较高的最大抑制比,与它们相比,老年猴无明显方位偏好细胞的最大抑制比显著降低; 实验3:戊巴比妥钠注射后,在年轻和中年大鼠上,alpha (8-12 Hz) 和beta (12-20 Hz) 频段EEG功率增加,theta (4-8 Hz) 功率减少,这些变化在中年大鼠上较为明显。氯胺酮注射后,中年大鼠theta功率比年轻大鼠具有更大幅度的降低。 我们的结果表明,视觉皮层功能的下调在衰老早期就已发生,其机制可能与抑制系统功能普遍降低有关. 2 奖赏机制,极低频磁场的生物学效应研究 本章首先对自然奖赏和药物成瘾机制、极低频磁场生物学效应,以及极低频磁场对奖赏系统的影响进行了综述,然后报道了作者的博士论文研究工作。实验目的是探讨大鼠眶额叶皮质(orbitofrontal cortex;OFC)活动与食物奖赏刺激的相关性,以及极低频磁场对小鼠空间认知能力的影响。 实验1采用EEG记录技术,检测了大鼠OFC在食物奖赏和渴求过程中EEG各频段的功率变化。在实验2中,使用了一种探索型Y-迷宫实验范式,它仅依赖于啮齿类动物天生的探索欲望,避免了奖赏效应的干扰,利用此新型迷宫,我们检测了25和50 Hz磁场对小鼠空间识别记忆能力的影响。其结果如下: 实验1:大鼠OFC的delta频段(2-4 Hz)EEG活动与食物刺激显著相关,其相对功率在食物渴求时下降,在食物奖赏时升高。 实验2:与短时照射相比,长时的50 Hz磁场照射降低了小鼠对新异臂的探索能力,而25和50 Hz磁场暴露都不影响小鼠的活动力。 本研究表明,食物奖赏与OFC的delta频段EEG活动密切相关,而我们以前发现,大鼠和猴OFC的gamma(20-100 Hz)活动与吗啡成瘾相关,提示了OFC在自然奖赏和药物成瘾中具有不同的作用;另外,本实验首次证明,极低频磁场损害了小鼠不依赖于奖赏系统的空间认知能力,而我们先前发现,极低频磁场可以强化吗啡诱导的条件化位置偏好,从而说明极低频磁场对吗啡成瘾具有独特的生物学效应。
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Since the 19th century, people have long believed that the function of cerebellum was restricted to fine motor control and modulation. In the past two decades, however, more and more studies challenged this traditional view. While the neuroanatomy of the cerebellum from cellular to system level has been well documented, the functions of this neural organ remain poorly understood. This study, including three experiments, attempted to further the understanding of cerebellar functions from different viewpoints. Experiment One used the parametric design to control motor effects. The activation in cerebellum was found to be associated with the difficulty levels of a semantic discrimination task, suggesting the involvement of the cerebellum in higher level of language functions. Moreover, activation of the right posterior cerebellum was found to co-vary with that of the frontal cortex. Experiment Two adopted the cue-go paradigm and event-related design to exclude the effects of phonological and semantic factors in a mental writing task. The results showed that bilateral anterior cerebellum and cerebral motor regions were significantly activated during the task and the hemodynamic response of the cerebellum was similar to those of the cerebral motor cortex. These results suggest that the cerebellum participates in motor imagination during orthographic output. Experiment Three investigated the learning process of a verb generation task. While both lateral and vermis cerebellum were found to be activation in the task, each was correlated a separate set of frontal regions. More importantly, activations both in the cerebellum and frontal cortex decreased with the repetition of the task. These results indicate that the cerebellum and frontal cortex is jointly engaged in some functions; each serves as a part of a single functional system. Taken these findings together, the following conclusions can be drawn: 1.The cerebellum is not only involved in functions related to speech or articulation, but also participates in the higher cognitive functions of language. 2.The cerebellum participates in various functions by supporting the corresponding regions in cerebral cortex, but not directly executes the functions as an independent module. 3.The anterior part of cerebellum is related to motor functions, whereas the posterior part is involved in cognitive functions. 4.While the motor functions rely on the engagement of both sides of the cerebellar hemispheres, the higher cognitive functions mainly depend on the right cerebellum.