Simultaneous and ultrarapid determination of reactive oxygen species and reduced glutathione in apoptotic leukemia cells by microchip electrophoresis

A microchip electrophoresis method coupled with laser-induced fluorescence (LIF) detection was established for simultaneous determination of two kinds of intracellular signaling molecules (reactive oxygen species, ROS, and reduced glutathione, GSH) related to apoptosis and oxidative stress. As the probe dihydrorhodamine-123 (DHR123) can be converted intracellularly by ROS to the fluorescent rhodamine-123 (Rh123), and the probe naphthalene-2,3-dicarboxaldehyde (NDA) can react quickly with GSH to produce a fluorescent adduct, rapid determination of Rh-123 and GSH was achieved on a glass microchip within 27 s using a 20 mm borate buffer (pH 9.2). The established method was tested to measure the intracellular ROS and GSH levels in acute promyelocytic leukemia (APL)-derived NB4 cells. An elevation of intracellular ROS and depletion of GSH were observed in apoptotic N134 cells induced by arsenic trioxide (AS(2)O(3)) at low concentration (1-2 mu m). Buthionine sulfoximine (BSO), in combination with AS(2)O(3) enhanced the decrease of reduced GSH to a great extent. The combined treatment of AS(2)O(3) and hydrogen peroxide (H2O2) led to an inverse relationship between the concentrations of ROS and GSH obtained, showing the proposed method can readily evaluate the generation of ROS, which occurs simultaneously with the consumption of the inherent antioxidant.

Observation of Three-Quasiparticle Doublet Bands in I-123: Possible Evidence of Chirality

A new band in the odd proton nucleus I-123 is identified via in- beam gamma- ray spectroscopy using the N-14+Cd-116 reaction. This band shows up as doublets with the previously assigned pi g(7/2) circle times (nu h(11/2))(2) band. Possible configurations of the new band are discussed in the framework of the cranked shell model and the geometrical model. It is argued that the new band might be a chiral partner of the previously known pi g(7/2) circle times (nu h(11/2))(2) band.

土壤传热特性的实验研究(Experimental Study of Soil Heat Diffusivity)

发展了测定实验室土样热扩散率的方法,介绍了研制的实验装置和建议的操作程序。给出的实验结果表明土壤热扩散率随土壤空隙率、含水量和温度等许多参数而变化。

Molecular dynamics simulation of hydrogen enhancing dislocation emission

The interactive pair potential between Al and H is obtained based on the ab initio calculation and the Chen-Mobius 3D lattice inversion formula. By utilizing the pair potentials calculated, the effects of hydrogen on the dislocation emission from crack tip have been studied. The simulated result shows that hydrogen can reduce the cohesive strength for Al single crystal, and then the critical stress intensity factor for partial dislocation emission decreases from 0.11 MPa root m (C-H = 0) to 0.075 MPa root m (C-H=0.72%) and 0.06 MPa root m (C-H = 1.44%). This indicates thar hydrogen can enhance the dislocation emission. The simulation also shows that atoms of hydrogen can gather and turn into small bubbles, resulting in enhancement of the equilibrium vacancy concentration.

Numerical Study Of Heat Transfer Mechanism In Turbulent Supercritical Co2 Channel Flow

Direct numerical simulation (DNS) of supercritical CO2 turbulent channel flow has been performed to investigate the heat transfer mechanism of supercritical fluid. In the present DNS, full compressible Navier-Stokes equations and Peng-Robison state equation are solved. Due to effects of the mean density variation in the wall normal direction, mean velocity in the cooling region becomes high compared with that in the heating region. The mean width between high-and low-speed streaks near the wall decreases in the cooling region, which means that turbulence in the cooling region is enhanced and lots of fine scale eddies are created due to the local high Reynolds number effects. From the turbulent kinetic energy budget, it is found that compressibility effects related with pressure fluctuation and dilatation of velocity fluctuation can be ignored even for supercritical condition. However, the effect of density fluctuation on turbulent kinetic energy cannot be ignored. In the cooling region, low kinematic viscosity and high thermal conductivity in the low speed streaks modify fine scale structure and turbulent transport of temperature, which results in high Nusselt number in the cooling condition of the supercritical CO2.

非线性地基上弹性梁屈曲传播抑制的动力有限元分析

基于非线性地基弹性梁屈曲传播的动态控制方程, 考虑梁质量惯性的影响, 用有限元和时间积分相结合的方法, 计算和分析了止屈器对梁屈曲传播抑制的作用. 止屈器是通过局部加大地基刚度加以模拟. 研究表明, 考虑动态得到的止屈效果和前人以准态为前提的结果相比有明显不同.

液晶热图技术在脉冲风洞中的应用

在短时间脉冲风洞中,对液晶热图实验技术进行了试验。在实验时间为20ms的炮风洞中,获得了清晰的三维高超声速分离流场的热图显示照片。热图照片不仅与油流图谱所显示的特征位置吻合,而且能半定量地显示表面热流的分布。该项技术的研究为国内空气动力学实验研究提供了一项新的方法。

拉断后射流对钢靶的随机侵彻

聚能破甲弹的金属射流断裂后发生径向随机发散,导致破甲深度显著下降。本文分析了径向随机发散的规律,得出描述拉断后射流对钢靶随机侵彻深度的一阶非线性常微分方程,并给出了大炸高条件下的简化解。

磁场的对流再产生和扩散

根据双尺度平均场电动力学,研究一种特定的周期对流的磁场再产生和扩散效应。数值计算运动学发电机方程,给出旋转对流元中感应电动势图象,得出无量纲磁场再产生系数α′≈-0.4～+0.4;对流扩散系数β′≈0.05.所提供的α效应能满足符合太阳活动周期的α-ω发电机模型的要求,若取对流元特征旋转速度u_1为100m/s,对流元特征长度α为1000km计算得到的对流扩散效应较发电机理论和观测的要求约小20倍。需计入脉动磁场和平均流场的相互作用,进一步研究旋转对流元的扩散效应。

混流式水輪机叶片的設計和計算(一)

<正> Q——通过水輪机的总流量N——水輪机的出力(功率)H——水头g——重力加速度η——水輪机的效率n——叶片数ω——水轉机轉动的角速度ξ_k——水力摩損系数(?)——势函数((?)有时也代表骨綫在u,z坐标中的傾斜角)ψ——在u,z坐标系中的流函数h(z)——流层的相对厚度Φ——h~(1/2)•(?)ψ——1/h~(1/2)ψQ_i——每一流层中通过z=0处的每单位厚

Shock slip-relations for thermal and chemical nonequilibrium flows

This paper appears to be the first where the multi-temperature shock slip-relations for the thermal and chemical nonequilibrium flows are derived. The derivation is based on analysis of the influences of thermal nonequilibrium and viscous effects on the mass, momentum and energy flux balance relations at the shock wave. When the relaxation times for all internal energy modes tend to sere, the multi-temperature shock slip-relations are converted into single-temperature ones for thermal equilibrium hows. The present results can be applied to flows over vehicles of different geometries with or without angles of attack. In addition, the present single-temperature shock slip-relations are compared with those in the literature, and Some defects and limitations in the latter are clarified.

A finite-element analysis of viscoelastically damped sandwich plates

A finite element analysis associated with an asymptotic solution method for the harmonic flexural vibration of viscoelastically damped unsymmetrical sandwich plates is given. The element formulation is based on generalization of the discrete Kirchhoff theory (DKT) element formulation. The results obtained with the first order approximation of the asymptotic solution presented here are the same as those obtained by means of the modal strain energy (MSE) method. By taking more terms of the asymptotic solution, with successive calculations and use of the Padé approximants method, accuracy can be improved. The finite element computation has been verified by comparison with an analytical exact solution for rectangular plates with simply supported edges. Results for the same plates with clamped edges are also presented.

采用偏置射流预燃室稳定燃烧水煤浆的原理及应用研究

水煤浆作为经济型清洁燃料引起人们的长期关注。水煤浆偏置射流预燃室具有对浆种适应性强，稳燃性能好等特点。本文对偏置射流预燃室稳定燃烧水煤浆的原理进行了探讨，并对该燃烧技术用于处理造纸黑液的研究情况作了介绍。结果表明，采用低压多级雾化喷嘴对水煤浆进行雾化，形成的雾炬和偏置射流预燃室的流场有良好的匹配，在预燃室内形成大尺度的回流区，有利于煤浆的点火、稳燃和消除灰渣。