十三种硝基苯类化合物对大型蚤(Daphnia magna Straus)急性毒性试验

本文应用静水试验研究了十三种硝基苯类有机物对大型蚤（D、M）的急性毒性。初步证明了硝基苯类有机物因其取代基团的不同（-Cl_、-OH_、-OH_、-NO_2）或取代基团的位置不同（邻、间、对）而表现出对大型蚤的不同毒性,且从分子结构上分析了毒性差异的机理。试验结果表明,对二硝基苯的毒性最大,其24hLC50为0.98±0.03ppm。

低pH值对大型溞（Daphnia magna Straus）存活、生长和生殖的影响

在静置换水条件下，研究了低ｐＨ值对大型存活、生长和生殖的影响。结果表明，在２５±１℃下，急性试验低ｐＨ值对大型的２４ｈ和４８ｈＬＬ５０值及其９５％可信限分别为ｐＨ值４．６６±０．１９和４．９４±０．２０。慢性试验进行了１４天，对存活和生长，ｐＨ值１．７５有影响，ｐＨ值５．０没有影响；对生殖，ｐＨ值５．０有影响，ｐＨ值５．５没有影响。显示出低ｐＨ值对大型的毒性阈限在ｐＨ值５．００－５．５０之间。

试验用大型溞(Daphnia magna)的培养方法

<正> 由于大型溞(Daphnia magna)的生长周期短、易培养且是水生态系中的一种重要的代表性生物。因此,许多国际组织和国家都推荐用大型溞作为生态毒理试验的试验生物。然而,关于试验用的大型溞的培养方法多为互相传授,在文献中很少见到系统的报导。在许多有关大型溞毒性试验的操作规程中,如世界经济发展与合作组织(OECD)出版的。“化学物质试验方法指南”,只强调如何进行毒性试验及在试验中的注意事项,而对试验生物的要求只原则地提到要选用健康的个体。因此,在实际操作中常常遇到一些困难,由于未能获得健康的个体而影

Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)

Our studies investigated the physico-chemical properties of alkaline phosphatase excreted by D. magna. This cladoceran mainly released alkaline phosphatase, though it also released a small amount of acid phosphatase. The alkaline phosphatase showed a broad pH optimum (8.05-10.0), and had a broad optimum temperature (30-35 degrees C) with a temperature coefficient (Q(10)) of 2.45. The K-m of the enzyme is 0.15 +/- 0.02 mM when p-nitrophenyl phosphate is used as a substrate, and the V-max is 0.43 +/- 0.01 mu M pNP mg(-1) DW h(-1). Even though alkaline phosphatase had been incubated in chloroform saturated with WC medium for 13 days, its activity was 54% that of the original. The enzyme was strongly inactivated by EDTA, and appeared to be zinc dependent. The alkaline phosphatase activity remained constant when D. magna was fed different quantities of Chlorella sp. The sensitivity of D. magna phosphatase activity to phosphate was time-dependent. During the first 16 hrs, the enzyme was insensitive to phosphate addition, after 24 hrs incubation the enzyme became sensitive to phosphate addition.

Toxicity of cypermethrin to Daphnia magna HB

The acute toxic effect of the pesticide cypermethrin to Daphnia magna HB was examined. D. magna HB was exposed to cypermethrin at concentrations of 0, 1, 3, 5, 7, and 9 mg/L for 24 In. Data showed that the 24 h-LC50 of cypermthrin on D. magna HB was 4.81 mg/L. In contrast, the 24 h-LC50 of K2Cr2O7 (the national standard toxicant) to Daphnia magna was 0.38 mg/L in the current study. Results indicated that the Daphnia magna was very sensitive to pesticides. In addition, the effects of the culture condition(such as hardness, temperature and DO etc.) on Daphnia magna HB was also studied.

Long-term toxic impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the reproduction, sexual differentiation, and development of different life stages of Gobiocypris rarus and Daphnia magna

The sexual ratio of Gobiocypris rarus exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 17 beta -estradiol from embryo to sexually mature revealed feminization and overdevelopment of connective tissue in male fish gonad in 2-30 pg/L TCDD concentration range. Daphnia magna was not sensitive to the high dose of TCDD (0.1-1000 ng/ml), but the reproduction of D. magna treated with TCDD decreased after the 8th day. 7-Ethoxyresorufin-O-deethylase (EROD) activities in newly fertilized eggs of G. rarus exposed to TCDD dosage groups (1000-100,000 pg/L) were significantly induced and increased with TCDD concentrations at the early life stage, while no difference was found between low TCDD dosage groups (<100 pg/L), but a good relationship between the EROD activity and the TCDD concentration was observed during a long-term developmental stage. There was a pericardial edema formed in a 2-week yolk-sac at the concentration of 1000 pg/L TCDD. But in the exposure group (2 pg/L TCDD for 120 days), the cell nuclei of hepatocytes was far from the center and packed toward the cell membrane; the cristae of most mitochondria in the cell dropped and collapsed; the rough endoplasmic reticulum broke into fragments; and numerous lipid droplets formed in the cell. (C) 2001 Academic Press.

两种不同株铜绿微囊藻培养液对大型溞的毒性效应研究

为了探讨铜绿微囊藻对浮游甲壳动物溞类的毒害效应,试验选取了两种不同株系(产毒和非产毒)的藻种,采取利用其培养液的方法,以研究铜绿微囊藻在生长过程中向水体中释放的毒素或类毒素物质对溞类的效应以及比较不同株系间效应的差异性。研究结果表明:(1)试验所选铜绿微囊藻产毒和非产毒株系的培养液均能对大型溞(Daphnia magna)的生长繁殖构成影响,表现为低剂量起促进效应,高剂量起抑制效应;(2)铜绿微囊藻产毒株较非产毒株更能影响大型溞的存活;(3)铜绿微囊藻产毒和非产毒株对大型溞的生长影响差异不大,但对其繁殖却

用水毒理学方法评价家用洗涤剂的潜在危害

选用5种不同产地的常用类型的家用洗涤剂,用水毒理学方法研究它们对水生动物的毒性影响并由此判断对水生态系统的潜在危害.结果表明,在远低于日常使用量的浓度下,5种家用洗涤剂均对稀有鲫(Grobiocypris rarus)鱼苗和大型(Daphnia magna,HB)有急性毒性,离体条件下对鲫鱼(Carassius auratus)鳃ATP酶有较强的抑制作用;在无急性毒性浓度下暴露时对大型繁殖有明显影响;当暴露于家用洗涤剂对鱼24h半数致死浓度的5%时鲫鱼鳃ATP酶受到明显抑制;家用洗涤剂溶液存放一段时

一种耐高温大型溞HB的培养和生物学的初步研究

本文介绍了夏季在武汉鱼池所采的耐高温大型溞(Daphnia magna HB),对其生长繁殖及其实验室条件下溞的种群密度、食物、温度和光照强度对生长、发育的影响,并对其培养和保种方法进行了研究。连续3年实验室的培养表明,在以新鲜藻类为食物的情况下,此种溢在15—31℃时能正常生长、繁殖。在31℃时,其最大体长为3.65mm,最高怀卵量为26个。根据国际标准法组织所介绍的(ISO 6341)对标准毒物——重铬酸钾的敏感性试验,24hEC_(50)为0.92mg/L(20%)。

六六六对大型溞生态学的影响

在实验室条件下,测定了六六六对大型溞(Daphnia magna Straus)存活、生长和生殖的影响。在25℃时,以心跳停止为死亡标准,六六六(以丙体计)对大型溞48小时LC_(50)及其95%可信限为1.32±0.30ppm。以存活、生长和生殖为毒性标准,未觉察反应浓度(NOEC)为150ppb,最低觉察反应浓度(LOEC)为200ppb,其应用因子在0.11-0.15之间。内禀增长能力(r_m)是更为敏感的指标,六六六浓度要降低至50ppb才无明显影响。

The toxicities of single-celled Microcystis aeruginosa PCC7820 and liberated M-aeruginosa to Daphnia carinata in the absence and presence of the green alga Scenedesmus obliquus

We evaluated the toxic effect of Microcystis aeruginosa on Daphnia carinata King using survival rate, population growth rate, and body length. When fed Microcystis aerugionsa PCC7820 and liberated colonial Microcystis spp., all D. carinata died within five days. When fed a mixture of M. aeruginosa PCC7820 and the green alga Scenedesmus obliquus, the survival rate, population growth rate, and body length of D. carinata generally increased. The survival rates were all above 80% after ten days. However, with liberated colonial M. aeruginosa, the toxic effect on D. carinata was more pronounced, and only at higher concentration of S. obliquus did that toxic effect abate. Our results indicated that green algae could greatly weaken the toxic effect of cyanobacteria.

Effect of available food and temperature on the growth and reproduction of Daphnia rosea

Population parameters of Daphnia rosea were studied at various concentrations of Chlorella sp. (0.25, 0.75 and 3.0 mg C l(-1)) at several temperatures (20, 25, 28, and 30 degrees C) in the laboratory. Although there were some differences in the degrees of the effects of the various temperature-food combinations, both food and temperature exerted influences on almost all of the main population parameters of D. rosea. At a water temperature of 28 degrees C, growth and reproduction were reduced, and at the lowest food level (0.25 mgC l(-1)), reproduction failed. D, rosea did not survive at 30 degrees C in spite of abundant food supply, indicating that 30 degrees C is a physiological limit. A positive relationship between body length and brood size was recognized at high and medium food levels. The slope of the regression was the highest at the highest food level and at the lowest temperature (20 degrees C). The low food level exerted a negative influence on the net reproductive rate by lowering the size of egg-bearing females, by decreasing the brood size of each size class, by decreasing the brood number per female, and by increasing the period of empty brood chamber. High water temperature (28 degrees C) also exerted a negative influence on the net reproductive rate in a similar way. For the better understanding of the key factors driving the midsummer dynamics of daphnids in the field, it may be of crucial importance to compare the population parameters of the field populations with experimentally derived values under controlled conditions of food concentration and temperature.