重组竹叶青蛇毒纤溶酶原激活剂TSV-PA效应观察体内溶栓

】目的观察重组竹叶青蛇毒纤溶酶原激活剂一体内溶栓效应。方法利用家兔制备的脑血检 模型, 以生理盐水和尿激酶为对照, 用数字减影血管造影技术观察重组竹叶青蛇毒纤溶酶原激活剂飞体内溶 检效果。结果昭高剂量实验组喇再通时间为以内月州低剂量实验组止以内再 通。尿激酶组以刃再通时间为一以内生理盐水对照组没有观察到再通现象。实验动物后处 死解剖, 未观察到出血现象和其它异常现象。结论重组巧勺一在体内具有良好的溶检活性。

B itte r an d Sw e e t /Um am i Ta s te Re c ep to rs w ith D iffe ren tly Evo lu tio n a ry Pa thw ays

通过生物信息学和系统发育学分析,研究了苦味受体和甜味/鲜味受体的进化途径。结果显示,苦味受体 和甜味/鲜味受体在进化上具有远相关,并且具有不同的进化途径,提示这可能是导致这些受体具有不同功能,传 导不同味觉的原因。

静止和充气培养条件下短期紫外辐射对钝顶螺旋藻光化学效率的影响

为了研究短期太阳紫外辐射对钝顶螺旋藻的影响,作者将静止和充气培养的藻体,暴露在全波长太阳辐射PAB(PAR+UVA+UVB),去除UVB辐射PA(PAR+UVA)及切断所有紫外辐射的光合有效辐射P(PAR)三种光处理条件下,测定了其光化学效率的变化。结果表明,紫外辐射(UVP)及光合有效辐射(PAR)均能导致钝顶螺旋藻的光化学效率降低,表现出了明显的光抑制,但是,UVR可导致更大程度的光抑制。充气培养条件下,与早晨(07:00)初始值相比,PAR导致了11%~20%的光抑制,而UVR(PAB-P)所产生的

微生物降解邻苯二甲酸(O-PA)的动力学

<正> 苯二甲酸及其脂类在石油化工,特别是作为合成工业的增塑剂和韧化剂和在染料制造业中的重要性以及他们对江河湖海的广泛污染而越来越引起人们的关注。Kluwe研究了邻苯二甲酸脂的致癌影响。Kozumbo发现了这种脂类的致突变作用。Kaneshima发现,苯二甲酸浓度在500—1000ppm时毒性甚大;Wilson指出,苯二甲酸钠在500ppm以上时有很大毒性。所以,关于苯二甲酸在天然环境里归趋的研究便相继进行。Ribbons等用测压技术研究了土壤假单胞菌类对苯二甲酸的氧化代谢,证明4,5-二羟基苯二甲酸为其

Vertical mixing within the epilimnion modulates UVR-induced photoinhibition in tropical freshwater phytoplankton from southern China

1. The importance of vertical mixing in modulating the impact of UVR on phytoplankton photosynthesis was assessed in a tropical, shallow lake in southern China from late winter to mid-spring of 2005. 2. Daily cycles of fluorescence measurements (i.e. photosynthetic quantum yield, Y) were performed on both 'static' and in situ samples. Static samples were of surface water incubated at the surface of the lake under three radiation treatments - PAB (PAR + UVR, 280-700 nm), PA (PAR + UV-A, 320-700 nm) and P (PAR, 400-700 nm). In situ samples were collected every hour at three different depths - 0, 0.5 and 1 m. 3. The general daily pattern was of a significant decrease in Y from early morning towards noon, with partial recovery in the afternoon. Samples incubated under static conditions always had lower Y than those under in situ conditions at the same time of the day. 4. Under stratified conditions, no overall impact of UVR impact could be detected in situ when compared with the static samples. Further rapid vertical mixing not only counteracted the impact of UVR but also stimulated photosynthetic efficiency. 5. Based on these measurements of fluorescence, the mixing speed of cells moving within the epilimnion was estimated to range between 0.53 and 6.5 cm min(-1). 6. These data show that mixing is very important in modulating the photosynthetic response of phytoplankton exposed to natural radiation and, hence, strongly conditions the overall impact of UVR on aquatic ecosystems.

Effects of solar ultraviolet radiation on photosynthesis of the marine red tide alga Heterosigma akashiwo (Raphidophyceae)

In order to assess the short- and long-term impacts of UV radiation (LTVR, 280-400 nm) on the red tide alga, Heterosigma akashiwo, we exposed the cells to three different solar radiation treatments (PAB: 280-700 rim, PA: 320-700 nm, R 400-700 nm) under both solar and artificial radiation. A significant decrease in the effective quantum yield () during high irradiance periods (i.e., local noon) was observed, but the cells partially recovered during the evening hours. Exposure to high irradiances for 15, 30, and 60 min under a solar simulator followed by the recovery (8 h) under dark, 9 and 100 mu mol photons m(-2) s(-1) of PAR, highlighted the importance of the irradiance level during the recovery period. Regardless the radiation treatments, the highest recovery (both in rate and total Y) was found at a PAR irradiance of 9 mu mol photons m(-2) s(-1), while the lowest was observed at 100 mu mol photons m(-2) s(-1). In all experiments, PAR was responsible for most of the observed inhibition; nevertheless, the cells exposed only to PAR had the highest recovery in any condition, as compared to the other radiation treatments. In long-term experiments (10 days) using semi-continuous cultures, there was a significant increase of UV-absorbing compounds (UVabc) per cell from 1.2 to > 4 x 10(-6) mu g UVabc cell(-1) during the first 3-5 days of exposure to solar radiation. The highest concentration of UVabc was found in samples exposed in the PAB as compared to PA and P treatments. Growth rates (mu) mimic the behavior of UV-absorbing compounds, and during the first 5 days mu increased from < 0.2 to ca. 0.8, and stayed relatively constant at this value during the rest of the experiment. The inhibition of the Y decreased with increasing acclimation of cells. All our data indicates that H. akashiwo is a sensitive species, but was able acclimate relatively fast (3-5 days) synthesizing UV-absorbing compounds and thus reducing any impact either on photosystem 11 or on growth. (c) 2006 Published by Elsevier B.V.

Effects of solar UV radiation on morphology and photosynthesis of filamentous cyanobacterium Arthrospira platensis

To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which had been grown under sunlight for decades) while they were cultured with three solar radiation treatments: PAB (photosynthetically active radiation [PAR] plus UVR; 280 to 700 nm), PA (PAR plus UV-A; 320 to 700 nm), and P (PAR only; 400 to 700 nm). Solar UVR broke the spiral filaments of A. platensis exposed to full solar radiation in short-term low-cell-density cultures. This breakage was observed after 2 h for the indoor strain but after 4 to 6 h for the outdoor strain. Filament breakage also occurred in the cultures exposed to PAR alone; however, the extent of breakage was less than that observed for filaments exposed to full solar radiation. The spiral filaments broke and compressed when high-cell-density cultures were exposed to full solar radiation during long-term experiments. When UV-B was screened off, the filaments initially broke, but they elongated and became loosely arranged later (i.e., there were fewer spirals per unit of filament length). When UVR was filtered out, the spiral structure hardly broke or became looser. Photosynthetic 0, evolution in the presence of UVR was significantly suppressed in the indoor strain compared to the outdoor strain. UVR-induced inhibition increased with exposure time, and it was significantly lower in the outdoor strain. The concentration of UV-absorbing compounds was low in both strains, and there was no significant change in the amount regardless of the radiation treatment, suggesting that these compounds were not effectively used as protection against solar UVR. Self-shading, on the other hand, produced by compression of the spirals over adaptive time scales, seems to play an important role in protecting this species against deleterious UVR. Our findings suggest that the increase in UV-B irradiance due to ozone depletion not only might affect photosynthesis but also might alter the morphological development of filamentous cyanobacteria during acclimation or over adaptive time scales.

基于PAR的高可信装备保障算法推演

算法被誉为计算的“灵魂”（spirit of computing），算法程序的可靠性和正确性对软件系统的可信度起着至关重要的作用。动态规划、贪心、分支限界等传统的算法设计策略缺乏有效的选择标准，一些算法形式化开发方法和工具的应用水平和范围也很有限，因此算法的可靠性已成为高可信软件系统的一个主要瓶颈。 作为军事运筹学的一个重要分支，装备保障计算主要研究军事活动中装备领域的决策优化问题，保障计算软件特别是算法的可靠性在很大程度上决定了装备保障的效率乃至军事行动的成败。利用形式化软件工程的研究成果、特别是引入算法程序的形式化开发方法，对我军通用装备保障领域中的大量算法类问题进行有效求解，有利于优化保障结构、统筹保障资源、整合保障力量、提高保障时效，促进装备整体战斗力水平的提升。 本文以高可信软件开发方法PAR为基础，面向装备保障计算的领域需求，提出了一类离散最优化问题（discrete optimization problem, DOP）的结构模型和算法推演技术，并成功推演了一系列典型的装备保障算法。本论文的主要创新性贡献如下： （1）定义了DOP的结构模型，提出了基于单点结构（Singleton）的问题分划递推策略，进而通过PAR算法推演生成问题的高效求解算法，涵盖了多种传统算法设计策略，显著提高了算法程序设计的机械化水平。 （2）提出了描述DOP分划递推过程的问题简约图（problem reduction graph, PRG）模型，并针对典型DOP结构推演得到了一组PRG构造算法模式，其中每个算法模式都涵盖了满足特定代数结构性质的一大类具体问题。 （3）阐述了PAR算法推演的范畴模型，为算法设计和重用提供了抽象而有效的方法指导。 （4）对我军通用装备保障领域具有代表性的48个问题进行了形式化的算法推演，构建了领域算法库。 （5）在PAR平台的基础上设计了装备保障算法开发平台的原型COPALM，支持DOP算法推演和重用，进而提高了相关应用软件的开发效率。

基于PAR的算法形式化开发

形式化方法是构建可信软件的重要途径.基于对算法问题的分析,针对形式化方法PAR开发算法的特征,刻划了问题分划、递推关系构造方面的规律.从一类问题的形式化功能规约出发,可机械地完成问题的分划及规约的变换,自然地揭示出求解问题的算法思想,在相关工具的支持下自动生成算法程序.研究结果将算法设计中尽可能多的创造性劳动转化为非创造性劳动,降低了形式化求解算法问题的难度,提高了算法程序的可靠性和形式化开发效率.

PAR方法和循环不变式的范畴语义

范畴论对理解程序规约及程序设计和正确性证明十分有用.PAR方法则是建立在严格的数学基础之上的一种统一的算法程序设计方法.循环不变式在循环算法程序的设计中至关重要.使用格理论和范畴论作为工具对PAR方法建立一个理论框架,并对其用范畴论的概念加以解释,从而使得PAR有更强的理论基础.在此基础上引入不动点原理深入刻划循环不变式的含义,循环不变式可以表示为谓词泛函的最小不动点,并从范畴论的角度解释该过程.