肉苁蓉寄生对梭梭幼苗水分生理特征及生长的影响

为研究肉苁蓉的寄生对其寄主梭梭幼苗水分生理代谢及其生长的影响,以寄生和未寄生肉苁蓉的梭梭为研究对象,分别测定其同化枝的含水量、自然饱和亏、离体同化枝保水力及蒸腾速率,同时测定了2种条件下梭梭的株高、地径、冠幅,并对其当年新生枝的生长量进行定期连续测定。结果表明:寄生肉苁蓉后,梭梭同化枝的组织含水量、相对含水量及保水力均小于未寄生肉苁蓉的梭梭,水分饱和亏增大,抗旱能力减弱。寄生肉苁蓉后,梭梭的蒸腾速率下降,其树高、地径、冠幅和新生枝生长量普遍小于未寄生肉苁蓉的梭梭。

Identification of Phenylethanoid Glycosides in Plant Extract of Plantago asiatica L. by Liquid Chromatography-Electrospray Ionization Mass Spectrometry

The present work describes a liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for rapid identification of phenylethanoid glycosides in plant extract from Plantago asiatica L. By using a binary mobile phase system consisting of 0.2% acetic acid and acetonitrile under gradient conditions, a good separation was achieved on a reversed-phase C-18 column. The [M-H](-) ions, the molecular weights, and the fragment ions of phenylethanoid glycosides were obtained in the negative ion mode using LC-ESI-MS. The identification of the phenylethanoid glycosides (peaks 1-3) in the extract of P. asiatica L. was based on matching their retention time, the detection of molecular ions, and the fragment ions obtained by collision-induced dissociation (CID) experiments with those of the authentic standards and data reported in the literature.