30 resultados para Caragana spp.

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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本文研究了毛乌素沙地6个不同生态环境的柠条群体和1个锡盟草原站的小叶锦鸡儿群体的同工酶和种子蛋白的遗传变异。利用Brown等(/975)同工酶遗传分析方法对同工酶和种子蛋白的多态性进行了遗传分析。运用Shannon信息指数和Nei指数分析测定了锦鸡儿群体的遗传结构。结果表明: (l) Brown同工酶遗传分析的方法适合于柠条同工酶的遗传分析。 (2用LAP酶对柠条群体进行了精细的遗传结构研究。结果表明毛乌素沙地柠条群体的异交性。GST=D.134,即大部分的变异存在于群体内部,小部分的变异存在于群体之间。柠条群体的繁育系统与水分胁迫有关。随着生境变旱,繁育系统有向近交变化的趋势。 (3) Brown同工酶遗传分析方法也可以运用于种子蛋白遗传分析,柠条种子蛋白各亚基的遗传是孟德尔方式。 (4)由种子蛋白变异所统计测定的柠条群体的遗传分化系数GST=0.l81(Nei指数)或0.179(Shannon指数,Kongkiatngam改进)。群体变异水平按表型多样性依次为人工毛条群体>锡盟群体>滩地群体>硬梁群体>软梁群体>丘下群体>丘上群体。按遗传多样性依次为人工毛条群体>软梁群体>锡盟群体>滩地群体和硬梁群体>丘上群体>丘下群体。群体间基因流水平Nm>/,遗传距离D<0.1。 还试验对玉兰、合欢、紫藤、绿豆几种植物种子蛋白变异的遗传分析。最后对同工酶,种子蛋白遗传分析和群体遗传结构进行了讨论并提出今后工作的一些建议。

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野大豆和锦鸡儿,同属豆科植物。前者是一年生自交植物,后者是多年生异交植物;一个是大豆的种质资源,另一个是固沙植物;一个具有耐盐适应,另一个能够抗旱。本文首先结合同工酶分析结果,通过随机扩增多态性DNA(RAPD]标记研究了锦鸡儿和野大豆群体的分子生态学特征。然后用限制性内切酶消化了单态的野大豆群体扩增产物以提高RAPD标记检测野大豆DNA多态性的能力。并且根据锦鸡儿群体的RAPD谱估算了每位点上的等位基因频率,分别用Shannon信息指数和Nei指数估测了各群体的遗传变异。最后,在以上研究的基础上结合我们实验室有关锦鸡儿和野大豆的形态,种子蛋白或同工酶方面的分析得到以下结论: 1]在本文的实验条件下,RAPD标记的重复性很好,使有关锦鸡儿和野大豆群体分子生态学的研究结果有了可靠的基础。 2]RAPD标记的显性特征使实验中确定显、隐性等位基因频率困难较大。用估测的显、隐性等位基因频率通过Shannon信息指数估算群体遗传多样性可能更适合于异交植物。 3)用限制性内切酶消化随机扩增产物后,能够提高RAPD标记检测野大豆群体DNA多态性的能力。4]按RAPD多态位点比率排列毛乌素沙地锦鸡儿各群体为:硬粱群体<沙丘群体<硬粱覆沙群体<毛条群体<滩地覆沙群体<软梁覆沙群体。按遗传多样性排列各群体为:硬粱群体<硬梁覆沙群体<沙丘群体<软梁覆沙群体<毛条群体<滩地覆沙群体。反映了RAPD多态位点比率与遗传多样性在检测群体DNA多态性能力上的异同。 5]毛乌素沙地锦鸡儿群体间 存在着强大的基因流,其高度异交性与生态过渡带可能是一致的。 6]根据同工酶、种子蛋白的分析结果,硬粱覆沙群体的基因多样性在毛鸟素沙地锦鸡儿各群体中是最高的。然而,根据DNA多态性的研究结果,硬粱覆沙群体的基因多样性在各个群体中仅大于硬梁群体。反映了锦鸡尔表型分化与基因型分化的差异。7]就本文的研究结果来看,野大豆群体以其高水平的遗传多样性和发育变通性适应着多变的盐环境。8]无论从DNA多态位点比率还是群体的基因多样性来看,异交植物锦鸡儿群体都具有比自交植物野大豆群体较高的水平。除了盐适应的野大豆群体外,一般来讲,锦鸡儿群体间的基因流要明显地大干野大豆的群体间的基因流。

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A parallel strategy for solving multidimensional tridiagonal equations is investigated in this paper. We present in detail an improved version of single parallel partition (SPP) algorithm in conjunction with message vectorization, which aggregates several communication messages into one to reduce the communication cost. We show the resulting block SPP can achieve good speedup for a wide range of message vector length (MVL), especially when the number of grid points in the divided direction is large. Instead of only using the largest possible MVL, we adopt numerical tests and modeling analysis to determine an optimal MVL so that significant improvement in speedup can be obtained.

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分离自新疆干旱荒漠地区的固氮蓝藻Nostoc spp.被证明具有较高的乙炔还原活性和特异抗逆性。为了保存Nostoc spp.的优良遗传性状和今后研究所需,我们以EMBL4噬菌体为载体,以Sau3AI部分酶解的15-22kb DNA片段为供体,构建了Nostoc spp.的基因组文库。以克氏肺炎杆菌和Anabaena sp. PCC7120的nifHDK DNA片段为探针,通过三轮噬菌斑原位杂交,成功地从基因文库中分离到了六个重组噬菌体阳性克隆。Southern杂交验证插入片段含有Nostoc spp.的固氮酶结构基因序列,并对其限制酶切图谱作了初步分析。

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Because the excreted sporocysts and/or oocysts of various species of Sarcocystis may not be discriminated morphologically, we sought to validate a diagnostic technique based on variation in the 18S rDNA sequence. Oocysts and/or sporocysts; from three taxa

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Khawia saurogobii n. sp. is described from the intestine of the cyprinid fishes Saurogobio dabryi and Saurogobio dumerili in China. The new species belongs to the Lytocestidae and is placed in Khawia because of its afossate scolex, vitelline follicles in the cortical parenchyma, uterus not looping anterior to the cirrus sac, gonopores separate but close together in distinct genital atrium, external seminal vesicle absent, and postovarian follicles present. The new species differs from other congeneric species by the shape of the body markedly tapering posteriorly from the end of the anterior third, the shape of the scolex that is very short and markedly wider than the neck, spatulate without incisions, but shallow superficial grooves, vitelline follicles and testes starting immediately posterior to the scolex, and an ovary with long, posterior arms bent medially, thus giving the shape of an inverted A.