32 resultados para Ca2 -activated K Channels

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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(batch method):(0.02mmolL-1)Ca2+Mg2+Na+1.0mmolL-18mmolL-1,Ca2+,Mg2+,K+,Na+,Si,Al3+Ca2+Mg2+,Na+K+

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(NostocflagelliformeBorn.etFlah.).,.25oC45mol/(m2s);N,PCa2+,AsK.,;,,15~45mmol/(m2s)

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The sediment redox potential was raised in the laboratory to estimate reduction of internal available phosphorus loads, such as soluble reactive phosphorus (SRP) and total phosphorus (TP), as well as the main elements of sediment extracts in Dianchi Lake. Several strongly reducing substances in sediments, which mainly originated from anaerobic decomposition of primary producer residues, were responsible for the lower redox potential. In a range of -400 to 200 mV raising the redox potential of sediments decreased TP and SRP in interstitial water. Redox potentials exceeding 320 mV caused increases in TP, whereas SRP maintained a relatively constant minimum level. The concentrations of Al, Fe, Ca2+, Mg2+, K+, Na+ and S in interstitial water were also related to the redox potential of sediments, suggesting that the mechanism for redox potential to regulate the concentration of phosphorus in interstitial water was complex.

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,.,,,Ca2+Mg2+K+3.,,-NH,.,.

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,pH,pH:(1),0.29 g/kg,101.56 g/kg,(EC)0.53 mS/cm,(EC>0.50 mS/cm);(2),6,pH6.55.5,pH(5.52),;(3)NO3-SO42-Cl-Ca2+Mg2+K+Na+,HCO3-,NO3-SO42-pH,Ca2+Na+pH

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,0-100 cm6,,pH,;,K+,Na+,Ca2+,Mg2+,K+,Na+,Ca2+,Mg2+

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Ca2+Mg2+K+Na+(BS)(CEC)(EC),Ca2+(pH2.5)Mg2+K+Na+BS(pH2.5)CEC(pH3.5),;C a2+Mg2+K+Na+,pH2.5Ca2+Mg2+K+Na+;Ca2+BSCEC(BSP)EC,pH 2.5,Ca2+BSBSPpH 3.5,pH4.5,

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Radial distribution function of CaCl2-KCl (1:2 mol) melt was measured by X-ray scattering of high temperature liquid. The nearest neighbour distances of Ca2+-Cl-, K+-Cl- and Cl--Cl- ionic pairs are 0.278, 0.306 and 0.380 nm, respectively, Discussion on the relation between structure and physicochemical properties in the melt was simply done in this paper.

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The Grove Mountains, including 64 nunataks, is situated on an area about 3200km2 in the inland ice cap of east Antarctica in Princess Elizabeth land (72o20'-73101S, 7350'-75o40'E), between Zhongshan station and Dome A, about 450km away from Zhongshan station (6922'S, 7622'E). Many workers thought there was no pedogenesis in the areas because of the less precipitation and extreme lower temperature. However, during the austral summer in 1999-2000, the Chinaer 16 Antarctic expedition teams entered the inland East Antarctica and found three soil spots in the Southern Mount Harding, Grove Mountains, East Antarctica. It is the first case that soils are discovered in the inland in East Antarctica. Interestingly, the soils in this area show clay fraction migration, which is different from other cold desert soils. In addition, several moraine banks are discovered around the Mount Harding. The soil properties are discussed as below. Desert pavement commonly occurs on the three soil site surfaces, which is composed of pebbles and fragments formed slowly in typical desert zone. Many pebbles are subround and variegated. These pebbles are formed by abrasion caused by not only wind and wind selective transportation, but also salt weathering and thaw-freezing action on rocks. The wind blows the boulders and bedrocks with snow grains and small sands. This results in rock disintegration, paved on the soil surface, forming desert pavement, which protects the underground soil from wind-blow. The desert pavement is the typical feature in ice free zone in Antarctica. There developed desert varnish and ventifacts in this area. Rubification is a dominant process in cold desert Antarctic soils. In cold desert soils, rubification results in relatively high concentrations of Fed in soil profile. Stained depth increases progressively with time. The content of Fed is increasing up to surface in each profile. The reddish thin film is observed around the margin of mafic minerals such as biotite, hornblende, and magnetite in parent materials with the microscope analyzing on some soil profiles. So the Fed originates from the weathering of mafic minerals in soils. Accumulations of water-soluble salts, either as discrete horizons or dispersed within the soil, occur in the soil profiles, and the salt encrustations accumulate just beneath surface stones in this area. The results of X-ray diffraction analyses show that the crystalline salts consist of pentahydrite (MgSO4-5H2O), hexahydrite (MgSO4-6H2O), hurlbutite (CaBe2(PO4)2), bloedite (Na2Mg(S04)2-4H2O), et al., being mainly sulfate. The dominant cations in 1:5 soil-water extracts are Mg2+ and Na+, as well as Ca2+ and K+, while the dominant anion is SO42-, then NO3-, Cl- and HCO3-. There are white and yellowish sponge materials covered the stone underside surface, of which the main compounds are quartz (SiO2, 40.75%), rozenite (FeSOKkO, 37.39%), guyanaite (Cr2O3-1.5H2O, 9.30%), and starkeyite (MgSO4-4H2O, 12.56%). 4) The distribution of the clay fraction is related to the maximum content of moisture and salts. Clay fraction migration occurs in the soils, which is different from that of other cold desert soils. X-ray diffraction analyses show that the main clay minerals are illite, smectite, then illite-smectite, little kaolinite and veirniculite. Mica was changed to illite, even to vermiculite by hydration. Illite formed in the initial stage of weathering. The appearance of smectite suggests that it enriched in magnesium, but no strong eluviation, which belongs to cold and arid acid environment. 5) Three soil sites have different moisture. The effect moisture is in the form of little ice in site 1. There is no ice in site 2, and ice-cement horizon is 12 cm below the soil surface in site 3. Salt horizon is 5-10 cm up to the surface in Site 1 and Site 2, while about 26cm in site 3. The differentiation of the active layer and the permafrost are not distinct because of arid climate. The depth of active layer is about 10 cm in this area. Soils and Environment: On the basis of the characteristics of surface rocks, soil colors, horizon differentiation, salt in soils and soil depth, the soils age of the Grove Mountains is 0.5-3.5Ma. No remnants of glaciations are found on the soil sites of Mount Harding, which suggests that the Antarctic glaciations have not reached the soil sites since at least 0.5Ma, and the ice cap was not much higher than present, even during the Last Glacial Maximum. The average altitude of the contact line of level of blue ice and outcrop is 2050m, and the altitude of soil area is 2160m. The relative height deviation is about 110m, so the soils have developed and preserved until today. The parental material of the soils originated from alluvial sedimentary of baserocks nearby. Sporepollen were extracted from the soils, arbor pollen grains are dominant by Pinus and Betula, as well as a small amount Quercus, Juglans, Tilia and Artemisia etc. Judging from the shape and colour, the sporepollen group is likely attributed to Neogene or Pliocene in age. This indicates that there had been a warm period during the Neogene in the Grove Mountains, East Antarctica.

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,:,,,,,HCO -3 > SO2 -4 > Ca2 + >Mg2 + > K+ NO-3 Cl - > Na + > NH+4 > PO3 -4 ,HCO32Ca ;,PO3 -4 ,Ca2 + NO-3 ,,,HCO-3 Ca2 + Mg2 + ,K+ NH+4 ;,HCO-3 Mg2 + ,,NH+4 NO-3 ,

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, 20042005. , SO2 -4 , NH +4 , Ca2 + , NO -3 H+, pH, . 4, SO2 -4 , NH+4 , NO -3 , Ca2 +F- , K+Mg2 +, Na+Cl- , H+.

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TSPSO220051120051231TSPF-Cl-SO42-NO3-NH4+K+Na+Ca2+Mg2+TSP 1. TSP36.91313.44µg/m3106.60µg/m3SO42-> NH4+> Ca2+ > NO3-> K+ > Na+ > Cl- > Mg2+ > F-SO42- (23.0412.16µg/m3)NH4+(3.052.23µg/m3)3730TSPTSPCl-SO42-NO3-NH4+K+Na+Ca2+Mg2+/:1.295.231.352.371.731.221.841.231.02 2. TSPTSPTSPTSPTSPTSPTSPTSPTSPTSPTSPTSPTSPTSPCa2+Mg2+F-(RH)SO42-NO3-NH4+Cl- 3. TSPTSPpH6.270.41Q0.82TSPSO42-TSP 4. NH4+SO42-NO3-R0.850.65NH4+SO42-0.8TSPNH4+(NH4)2SO4Ca2+Mg2+R=0.72 5. 2005TSPSO42-/ Ca2+13.64SO42-/ Ca2+20NO3-/SO42-0.15

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The characterization of acid-sensing ion channel (ASIC)-like currents has been reported in hippocampal neurons in primary culture. However, it is suggested that the profile of expression of ASICs changes in culture. In this study, we investigated the properties of proton-activated current and its modulation by extracellular Ca2+ and Zn2+ in neurons acutely dissociated from the rat hippocampal CA1 using conventional whole-cell patch-clamp recording. A rapidly decaying inward current and membrane depolarization was induced by exogenous application of acidic solution. The current was sensitive to the extracellular proton with a response threshold of pH 7.0-6.8 and the pH(50) Of 6.1, the reversal potential close to the Na+ equilibrium potential. It had a characteristic of acid-sensing ion channels (ASICs) as demonstrated by its sensitivity to amiloride (IC50 = 19.6 +/- 2.1 muM). Either low [Ca2+](0) or high [Zn2+](0) increased the amplitude of the current. All these characteristics are consistent with a current mediated through a mixture of homomeric ASIC1a and heteromeric ASIC1a + 2a channels and closely replicate many of the characteristics that have been previously reported for hippocampal neurons cultured for a week or more, indicating that culture artifacts do not necessarily flaw the properties of ASICs. Interestingly, we found that high [Zn2+] (>10(-4) M) slowed the decay time constant of the ASIC-like current significantly in both acutely dissociated and cultured hippocampal neurons. In addition, the facilitating effects of low [Ca2+](0) and high [Zn2+](0) on the ASIC-like current were not additive. Since tissue acidosis, extracellular Zn elevation and/or Ca2+ reduction occur concurrently under some physiological and/or pathological conditions, the present observations suggest that hippocampal ASICs may offer a novel pharmacological target for therapeutic invention. (C) 2004 Elsevier B.V. All rights reserved.

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