10 resultados para CD3
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
Partial cDNA sequences of TCR gamma and CD3 gamma/delta were isolated from the thymus of common carp (Cyprinus carpio L.) by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp TCR gamma and CD3 gamma/delta were obtained by means of 3' RACE and 5' RACE, respectively. The full length of carp TCR gamma chain is 1368 bp and encodes 326 amino acids including a signal peptide region of 19 amino acids and a transmembrane region of 23 amino acids at the C-terminal region from aa 291 to 313. The V region of carp TCR gamma contains 109 amino acids, the core motif FGXG in J segment was also found in carp TCR gamma. The C region of carp TCR gamma contains the characteristic CX6PX6WX45C motif. The CP region of carp TCR C gamma contains 37 amino acids. The full length of carp CD3 gamma/delta is 790 bp and encodes 175 amino acids including a signal peptide region of 17 amino acids and a transmembrane region of 23 amino acids from aa 93 to 115. Similar to other known CD3 gamma/delta s, four cysteine residues in the extracellular domain and an immunoreceptor tyrosine-based activation motif ITAM (YxxL/Ix6-8YxxL/I) in the intracellular domain are also included in carp CD3 gamma/delta. Differing from other known CD3 gamma/delta s, carp CD3 gamma/delta tacks the CXXCXE motif in the extracellular domain. RTPCR analysis demonstrated that the expression of TCR gamma gene was mainly in the thymus and gill of 6-month carp, but in 18-month carp, TCR gamma gene was detected in all the examined tissues. The expression of CD3 gamma/delta gene was detected in all examined tissues of 6 and 18-month carp; among them, the highest expression level was in the thymus of 6-month carp. In situ hybridization showed that CD3 gamma/delta-expressing cells were widely distributed in the head kidney, spleen and kidney of carp, whereas in the thymus, they were densely distributed in the lymphoid outer zone and scattered in the epithelioid inner zone. (c) 2007 Published by Etsevier Ltd.
Resumo:
TRBCTCD2CD3/TCRCD5CD6CD7CD2TRBC, ETRBCE, ETRBC, CD2TRBC1412
Resumo:
(CVF). BN,Lewis,.2,8.CVF:3 d2 d,CVF 50 g/kg;12 h,CVF 20 g/kg,2 d1.:.,1356d(CH50)C3CD3+T,. CVF(11.690.72)d(6.650.35)d,,(P0.01).CVFC3CD3+T,. CVF,.
Resumo:
13COZ1953S353TNKCD3CD4CD4CDSCDSS3TThTTsTlS3IL-2IL-1mRNA53ESI-MSK+KNO3nNO3-KNO3mn236m4
Resumo:
therapeutic drugs, vaccines and mechanisms of human diseases. Little is known about the normal levels of leukocyte subpopulations of Chinese rhesus macaques. To obtain these data, 100 blood samples from Chinese rhesus macaques were collected. The normal range of major leukocyte subpopulations, such as T lymphocytes, B lymphocytes, monocytes, myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs), were quantitatively analyzed by flow cytometry through BD trucount tubes. The influence of age and sex on the cell counts of leukocyte subpopulations was analyzed. The counts of CD3+ T cells, CD3+CD4+ T cells, CD3+CD8+ T cells and B cells decreased with age, but those of monocytes, mDCs and pDCs had no significant correlation with age. Significant differences existed in the cell counts of most leukocyte subpopulations between the male and female groups except pDCs. Furthermore the values of the females were higher than those of the males. The study provided basic information about the leukocyte subpopulations of Chinese rhesus macaques, and it may be valuable for immunobiological study of Chinese rhesus macaques. Cellular & Molecular Immunology. 2009;6(6):433-440.
Resumo:
1985T(Tupaia belangeri)(TRBC)(SRBC)(E)TRBCTRBCT45TRBCSRBC(CD2)T(CD)TCD(McAb)(Antigenic modulation or co-modulation)TRBCTRBCE~+-PBL88.8%E~--PBL4.16%TRBCT(CEM, H33JHJA1, Jurkat, MLA-144, Molt-3, Molt-4, Molt-4 clone-8 PEER)BB(Daudi, RajiReh)TCD3, TCR, CD5, CD6CD7McAb OKT3, T108(F1), T136(F101-15), T149(M-T604)T152(7G5)TRBCTRBCT11.1 McAb OKT11OKT11E49.3%77.7%1013CD2 McAbT089 (39C1.5)12McAbT081 (x/3)T082 (GLB-T11.2/1)T083 (GLB-T11.1/1)T085 (RPA-2.10)T1088 (0-275)T092 (M-T201)TRBCET084 (F110.08)T091 (AICD2.1)CD2 McAb-T086 (D-66 clonel)T087 (GT-2)TRBCTRBCEEET090 (6F10.3)T198 (JOR-T2)TRBCETRBCTTCD2 (gp50), CD3/TCRCD5, CD6CD7CD2TRBCEETRBCETRBCTRBCT
Resumo:
,60%S3,,.:,S3;3H-TdR,S3ConA;3H-TdR,S3NK;ELISAS3IL-2;,S3TCD3CD4CD8CD4/CD8;RT-PCRIL-2 mBNA.:S3(P<0.05);ConA(P<0.001);NKIL-2(P<0.001,P<0.01);CD8(P<0.001),CD4/CD8(P<0.001);IL-2mRNA,(P<0.05).:S3,.
Resumo:
CD3 ODH/D [M +1]+ [M +2 ]+ [M +3]+ (CID)H/D
Resumo:
ObjectiveDeveloping a generalized psychological intervention program, and explore its influence on the emotion, subjective health, and immunity function of the perioperation patients with breast cancer. MethodSixty patients with breast cancer were randomly divided into intervention and control groups. The clinical psychological intervention was performed on patients in the intervention group for 20 days, in addition to the routine therapy and care. Levels of emotion (SAS & SDS), subjective health (SF-36), and immunity function (t lymphocyte subsets) of the patients were tested. Results: 1.There was no significant difference between the age, income, educational level, and type of prefession of the two groups. There was no significant difference between SAS, SDS, SF-36 and lymphocyte subsetsCD3+, CD4+, CD8+, CD4+/CD8+, NK of the two groups. 2. Scores of SAS and SDS decreased significantly after intervention in experimental group, while the score of SF-36, the average value of CD4+, CD4+/CD8+, and NK increased significantly. For the control group, the score of depression decreased significantly after intervention, while the score of PF, GH, VT, SF, RE, and MH increased significantly. 3. In comparison of the intervention and control group, the intervention effect of SAS, SDS, SF-36 scores (except SF), CD3+, CD4+, CD4+/CD8+, and NK differed significantly, with the priority of experimental group. 4. SDS, SAS, and CD3+, CD4+, NK correlated in negative respectively, while SDS, SAS, and CD8+ correlated in positive. PF, RP, GH, SF, and MH of subjective health correlated in positive with every index of immunity function in positive, except negative correlation with CD4+/CD8+. BP, RE correlated with CD3+,CD4+,CD8+, and NK in positive. VT correlated in positive with CD3+, CD8+, and NK, in negative with CD4+/CD8+. Conclusions: 1. Anxiety, depression, and subjective health, correlated with immunity function in perioperation patients with breast cancer. 2. Psychological intervention can improve the emotional status, subjective health, and immune function of patients with breast cancer to the optimum in perioperative period.
