Diterpenes, sesquiterpenes, and a C-15-acetogenin from the marine red alga Laurencia mariannensis

In addition to 10 known compounds (7-16), one new brominated diterpene, 10-hydroxykahukuene B (1), two new sesquiterpenes, 9-deoxyelatol (2) and isodactyloxene A (3), one new brominated C-15-acetogenin, laurenmariallene (4), and two new naturally occurring halogenated sesquiterpenes (5 and 6) that were previously obtained as intemediates in a biomimetic synthetic study of rhodolaureol and rhodolauradiol have been isolated and identified from the organic extract of the marine red alga Laurencia mariannensis. The structures of these compounds were established by spectroscopic methods. The antibacterial and antifungal activities of new compounds 1-4 were evaluated.

Halogenated Terpenes and a C-15-Acetogenin from the Marine Red Alga Laurencia saitoi

Seven parguerane diterpenes: 15-bromo-2,7,19-triacetoxyparguer-9(11)-en-16-ol (1), 15-bromo-2,7,16,19-tetraacetoxyparguer-9(11)-ene (2), 15-bromo-2,19-diacetoxyparguer-9(11)-en-7,16-diol (3), 15-bromo-2,16,19-triacetoxyparguer-9(11)-en-7-ol (4), 15bromo-2,16-diacetoxyparguer-9(11)-en-7-ol (5), 15-bromoparguer-9(11)-en-16-ol (6), 15-bromoparguer-7-en-16-ol (7), two polyether triterpenes: thyrsiferol (8) and thyrsiferyl 23-acetate (9), and one C15-acetogenin, neolaurallene (10), were isolated from a sample of marine red alga Laurencia saitoi collected off the coast of Yantai. Their structures were established by detailed NMR spectroscopic analysis and comparison with literature data.

Laurenidificin, a new brominated C-15-acetogenin from the marine red alga Laurencia nidifica

new brominated C-15-acetogenin, namely, laurenidificin, was isolated from the marine red alga Laurencia nidifica. Its structure was determined on the basis of spectroscopic methods. (C) 2010 Bin Gui Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

Laurendecumallenes A-B and laurendecumenynes A-B, halogenated nonterpenoid C-15-Acetogenins from the marine red alga Laurencia decumbens

Four new halogenated nonterpenoid C-15-acetogenins, 4:7,6:13-bisepoxy-9,10-diol-1,12-dibromopentadeca-1,2-diene (1, laurendecumallene A), 4:7,6:12-bisepoxy-9,10-diol-1,13-dibromopentadeca-1,2-diene (2, laurendecumallene 13), (3Z)-6:10,7:13-bisepoxy-12-bromo-9-hydroperoxylpentadeca-3-en-1-yne (3, laurendecumenyne A), and (3Z)-6:10,9:13-bisepoxy-12-bromo-7-chloropentadeca-3-en-1-yne (4, laurendecumenyne 13), together with one known halogenated C-15-acetogenin elatenyne (5) were isolated and identified from the organic extract of the marine red alga Laurencia decumbens. Their structures and relative stereochemistry were established by means of spectroscopic analysis including UV, IR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and ID and 2D NMR techniques. All these metabolites were submitted for the cytotoxic assay against tumor cell line A549 (human lung adenocarcinoma), but all of them were found inactive (IC50 > 10 mu g/mL).

镜像核~(13)N-~(13)C和~(15)N-~(15)O中的激发态晕或皮(英文)

用非线性相对论平均场对两对镜像核13N 13C和15N 15O进行了研究. 发现无论在基态还是激发态, 用两套参数所得的结合能都跟实验值很接近. 计算结果显示13N的第一激发态 (2s1 /2 )和第三激发态(1d5 /2 )各存在一个非束缚的质子晕, 而13C的第三激发态 (1d5 /2 )存在一个弱束缚的中子皮. 另外研究表明, 在另一对镜像核15N 15O的第二激发态 (2s1 /2 )和第一激发态 (2s1 /2 )分别存在一个中子晕和质子皮.

微/纳米氧化锌（ZnO）的生长、性质及其应用研究

纳米氧化锌（ZnO）是一种直接宽带隙半导体材料，室温下其禁带隙宽为本3.37 eV，激子束缚能为60 meV。纳米ZnO有明显的尺寸效应、表面和界面效应等，物理化学性能优越。在压电材料、铁电材料、平面显示、表面声波、传感器、场发射器件、激光、光催化等方面有着广泛的用途。近年来，对纳米ZnO材料的研究成为国内外的一个热点。 本论文研究了用化学气相沉积（CVD）法制备微/纳米ZnO材料。通过控制实验条件，合成了多种特殊结构和形貌的微/纳米ZnO材料，并用扫描电子显微镜（SEM）、高分辨率透射电镜（HR-TEM）、X-射线衍射仪（XRD）、Raman光谱和光致发光（PL）等对材料的结构和光学性能进行了表征。采用CVD法，在温度为630 °C，氧气流量为15 sccm，氩气流量为300 sccm的条件下，制备了一种纳米带冠四足状ZnO（T-ZnO）。此结构ZnO材料的每根足顶端均有一规则的六方帽形结构，具有很大的比表面积。实验结果表明：合成的ZnO材料为纤维锌矿结构单晶，并且沿着(0001)方向生长；室温下的PL谱有两个激发峰，一个是在393 nm处相对较弱的近带紫外峰，另一个是在511 nm处强峰。而材料在600 °C下氧气中退火30 min后，511 nm附近的绿光激发辐射峰则基本消失了，这说明在511 nm处的绿光激发辐射峰可能是由于氧空位引起的。此外，通过改变实验条件，还得到了其他多种结构的微/纳米ZnO材料。 通过大量实验，找到了一种在低温下合成微/纳米ZnO材料的新方法，即水蒸气氧化法。用ZnI2作为锌源，水蒸气作为氧化剂，实验温度在300～500 °C范围内，大大低于通常CVD法的500～1500 °C。采用此法，用硅做基底，得到了一系列有趣的实验结果，大多数情况下ZnO纳米晶自组装成很规则的圆。而在瓷舟中收集到的纳米ZnO跟普通CVD法结果相似，可以得到锥状、棒状等结构的纳米晶，但其生长方式与硅基底上的有很大差别。此外，用水蒸气氧化法，还实现了ZnO纳米晶在碳纳米管（CNTs）上的直接生长，而且其PL性能增强，这可能是纳米ZnO和CNTs相互耦合的结果。在700 °C温度下，以锌粉和ZnI2作为锌源，用水蒸气作为氧化剂，在硅基底的正反面分别得到了纳米棒和纳米推子阵列。此外，还对水蒸气氧化法的化学反应机理进行了分析，实验结果证明：固态ZnI2在受热和一定真空度下先蒸发成ZnI2分子，ZnI2分子遇到水蒸气发生反应生成偶极ZnO分子，这些ZnO偶极分子在硅基底上通过静电力自组装成特殊的几何形状。 此外，还通过分子动力学模拟的方法，对材料的力学性能进行了研究，得到了ZnO的弹性常数和体弹性模量，模拟值跟其他研究人员的实验和模拟结果吻合得很好，并估出算了ZnO晶体的表面能和断裂韧性。 本论文还对制备材料的光催化性能进行了系统的研究，采用CVD法制备ZnO，对铬黑T（EBT）进行光催化降解实验。通过正交实验方法，得到了ZnO催化降解EBT的最佳工艺条件，即催化剂用量为5 g/L，光照强度为120 W，反应温度为20 °C，反应时间为120 min，EBT浓度为10 mg/L，溶液pH值为4。 在最佳实验条件下，20分钟内有95%的EBT被降解完，30分钟内则全部降解。因此，ZnO在EBT的降解中催化效率很高，在废水处理中具有潜在的应用前景。

中国西北地区分布的几种荒漠植物的生理生态学特性研究

白沙蒿(Artemisia sphaerocephala Krasch.)是中国西北部沙漠的流动及固定沙丘上广泛分布的优势种灌木。瘦果小而轻;借助显微镜和扫描电镜对瘦果的形态结构进行了观察，其种子的种皮与果皮愈合，果皮外层为很厚的粘液层，其粘液层在遇湿后迅速吸水膨胀，其重量增至原来的589倍。在自然生境中，粘液物质将沙粒粘附于瘦果的周围，使瘦果的重量发生变化。瘦果能长时间在水上漂浮，粘液物质有助于种子的萌发和苗的发育。白沙蒿产生三种不同颜色的瘦果，其种子具有不同的萌发速率但最终达到相同的萌发率。瘦果的粘液物质对白沙蒿种子的传播与萌发具有重要的生态意义。白沙蒿种子为需光种子，种子在光下萌发而在暗中受到抑制。种子萌发的适宜温度为250 C，在100 C和300 C萌发速率和萌发率都很低，萌发在50 C受到抑制。种子在沙中被埋越深，其萌发速率和萌发率就越低。在沙平面下2厘米或更深层沙土下出苗率为零。但是，当将种子上层沙土移走，只保留0.5厘米沙土覆盖种子，这些种子的萌发率达到原先就位于沙土下0.5厘米的种子的萌发率，但是后者的萌发速率较高。土壤水分含量越高，从1. 7%到14. 7%，其萌发就越快。土壤水分含量从19.4%起，种子的萌发受到了延迟，苗的发育受到了抑制。 梭梭(Haloxylon ammodendron)是我国西北荒漠中分布的多年生植物，属中亚荒漠植被成分。其种子萌发的最适温度为loo C，亚适宜温度为15-20 0C。从200C起，温度越高，萌发比率越低。种子无论在光下和暗中都能萌发，萌发率无显著性差异。浓度低于0.2 molL的NaCl溶液对萌发的影响不大;但从0.8 mol/L起，萌发率随着浓度增高而降低，直至为零。将在盐溶液中处理9d的种子转移至蒸馏水后，原来较高浓度下的种子具有较高的萌发恢复率。不同浓度下的种子的萌发率和萌发恢复率要比蒸馏水中的低，表明NaCl处理后的部分种子永久地失去萌发力。梭梭的种子为短命种子，在自然状态下，种子的含水量为8.5%，寿命约为10个月。将梭梭种子含水量降至2. 5%和1.4%，其耐贮藏力增强。经过500 C下5天和10天的人工加速老化后，超干种子表现出较强的抗老化能力。与对照相比，超干种子萌发率、根长及活力指数等均显著提高。较低的电导率和较高的脱氢酶活性，说明种子在超干处理后表现出了膜系统的完整性和较高的种子生活力。超干处理既能显著提高梭梭种子的耐贮性，也能有效保持种质的稳定性，是梭梭种质资源保护的重要途径之一。 沙柳（Salix psammophila）是毛乌素沙地流动沙丘和固定沙丘上广泛分布的重要固沙植物。沙柳叶片的光合速率呈现出双峰型的日动态变化，在午后14点出现光合午休现象。蒸腾速率呈现出单峰型的日动态变化，蒸腾在午后15点达到最大。水分利用效率在早晨9点后保持在2.5 μmol C02．mmol-l H20左右。叶片水分亏缺也表现出规律性的变化。从早晨开始，叶片水分亏缺逐渐增强，在18时达到最大值。比较生长季节的不同时期环境因子的变化情况表明，春末的光照强度和气温比夏末高，但土壤水分含量则相反。沙柳在春末的光合速率、蒸腾速率分别是夏末的79%和72.4%，表明水分亏缺是影响沙柳生长发育的主要限制因素。

毛乌素沙地油蒿与籽蒿种子萌发对光照的反应

油蒿(Artemisia ordosica)与籽蒿(Artemisia sphaerocephala)为毛乌素沙地的优势物种，广泛用于我国北方干旱、半干旱区的生态恢复。油蒿与籽蒿种子萌发对温度、水分等环境条件的反应已有较为明确的研究结果，但关于这两种蒿属植物种子萌发对光照反应的研究目前存在两种不同的结论，对飞播技术的改善造成一定影响。 影响油蒿与籽蒿种子萌发对光照反应的因素较多。如种源地、结实部位、种子颜色、种子保存方式与时间、实验条件、温度等。考虑种源地、种子保存时间与方式、结实部位及温度，从多方面系统研究油蒿与籽蒿种子萌发对光照的反应。结果表明当温度较低时(10:20C)，萌发率和萌发速率在黑暗条件下显著高于在光照条件下。采自榆林地区的种子萌发状况相对较差，萌发率和萌发速率在大多数情况下显著低于其他两地;各种源地上下部种子萌发率有一定差异，大多数结实部位为上部的种子最终萌发率和萌发速率高于下部的种子;保存几周的种子萌发率变化不大，但在温度较高和较低时，随着种子保存时间的延长，萌发率下降较快，而在适宜温度下种子萌发率变化不明显;种子保存方式的不同造成种子萌发情况的差异，但差异不明显;黑暗条件下比近似黑暗条件下的种子萌发率略低。上述因素对种子萌发均具有一定影响，但总体特征是种子萌发对光照的反应表现为强光抑制种子的萌发，黑暗促进种子萌发。当温度较高时(15:25C)，光照与黑暗条件下种子萌发率差异不大，但黑暗条件下种子的萌发速率显著高于光照条件下;当其它条件相同时，种子萌发率和萌发速率显著高于温度较低时(10:20C)。因而，适宜的温度和黑暗条件有利于油蒿与籽蒿种子的萌发。在飞播实践时应为种子创造一定的沙埋条件，创造有利于种子萌发的黑暗环境，促进种子的萌发，提高飞播工作的成效。

海南黄牛和徐闻黄牛线粒体DNA的多态性及其品种分化关系

以14种限制性内切酶分析来自海南岛的海南黄牛和雷州半岛的徐闻黄牛的线粒体DNA限制性片段长度多态性(mtDNA RFLP)。结果只有一种限制性内切酶(sal I)在海南黄牛中检测到多态性, 且其中的C型(15.0, 1.3)尚未见报道。结果还显示, 两个品种6个个体的mtDNA基因单倍型全部表现为A型, 即瘤牛的血统, 并表明两个品种的亲缘关系很近。

Bacillus pallidus sp nov., isolated from forest soil

A Gram-positive bacterium, designated strain CW 7(T), was isolated from forest soil in Anhui Province, south-east China. Cells were strictly aerobic, motile with peritrichous flagella and rod-shaped. The strain grew optimally at 30-37 degrees C and pH 7.0-8.0. The major fatty acids of strain CW 7(T) were anteiso-C-15:0, iso-C-15:0 and anteiso-C-17:0. The predominant menaquinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The G + C content of the genomic DNA was 42.3 mol%. Phylogenetic analysis indicated that strain CW 7(T) belonged to a monophyletic cluster within the genus Bacillus and showed 16S rRNA gene sequence similarities of less than 96.5% to recognized species of the genus Bacillus. The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analyses, showed that strain CW 7(T) represents a novel species of the genus Bacillus, for which the name Bacillus pallidus sp. nov. is proposed. The type strain is CW 7(T) (=KCTC 13200(T)=CCTCC AB 207188(T)=LMG 24451(T)).

Sphingobacterium siyangense sp nov., isolated from farm soil

The taxonomic position of a novel Gram-negative strain, designated Sy1(T), isolated from a farm-soil sample obtained from Jiangsu Province, PR China, was characterized by using a polyphasic approach. The cells were non-motile, non-spore-forming rods. The organism grew optimally at 30-37 degrees C and at pH 6.0-8.0. Based on 16S rRNA gene sequence analysis, strain Sy1(T) is a member of the genus Sphingobacterium; Sphingobacterium multivorum JCM 21156(T) was the nearest relative (98.5% sequence similarity). The predominant fatty acids of strain Sy1T were isoC15:0 (32.90/o), C16:0 (10.9%) and summed feature 3 (iso-C-15:0 2-OH and/or C-16:1 omega 7c; 24.1%). The DNA G + C content was 38.5 mol%. The low level of DNA-DNA relatedness (2.2 %) to S. multivorum JCM 21156 T in combination with differential morphological and biochemical properties demonstrated that strain SY1(T) (=KCTC 22131(T)= CGMCC 1.6855(T)) should be classified as representing a novel species of the genus Sphingobacterium for which the name Sphingobacterium siyangense sp. nov. is proposed.

Flavobacterium anhuiense sp nov., isolated from field soil

A novel strain, D3(T), isolated from a field-soil sample obtained from Anhui Province, PR China, was characterized taxonomically by using a polyphasic approach. The cells were Gram-negative, yellow-pigmented rods devoid of flagella, but showing gliding motility. The organism was able to grow at 5-37 degrees C and at pH 4.0-10.0. A comparative 16S rRNA gene sequence analysis indicated that strain D3(T) is a member of the genus Flavobacterium, sharing highest sequence similarity with the type strain of Flavobacterium defluvii (96.7 %). The major isoprenoid quinone was MK-6 and the predominant fatty acids were iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH) and C-16:0. The DNA G + C content was 31.4 mol%. On the basis of phylogenetic and phenotypic data, strain D3(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium anhuiense sp. nov. is proposed. The type strain is D3(T) (=KCTC 22128(T)= CGIVICC 1.6859(T)).

细菌视紫红质的构象模拟和视黄醛的顺反异构模型

在细菌视紫红质构象模拟的基础上提出了一个视黄醛顺反异构模型。该模型中视黄醛的C~(13)—C~(14)与Lys—216的C~(δ)—C~(ε) 处于一直线上, 这样, 顺反异构只涉及视黄醛C~(15)和Lys—216的N~(ζ)绕此直线为轴的旋转。理论计算表明, 这是一种空间障碍最小的模型, 并能通过Asp—96和Asp—212传递质子。图3表2参10

Feeding rates affect heat shock protein levels in liver of larval white sturgeon (Acipenser transmontanus)

A two-week trial was conducted to study the effect of feeding rates on heat shock protein levels in larval white sturgeon. The larvae (30 day post hatch, 230 mg initial body weight) were fed a commercial feed (12.6% moisture, 49.5% crude protein. 20.7% Crude fat, and 8.6% ash) at 5, 15. or 25% body weight per clay (BW d(-1)). Liver heat shock proteins (Hsp) were measured before and after the larvae were subjected to a heat shock from 18 to 26 degrees C at 1 degrees C/15 min and maintained at 26 degrees C for 4 h thereafter. Before heat shock, larvae fed 5% BW d(-1) had significantly (P<0.05) lower final body weight, RNA/DNA ratio, whole body lipid and protein content, and Hsp60 and Hsp70 levels but higher protein efficiency ratio, and whole body moisture content than larvae fed the two higher feeding rates. Heat shock significantly induced Hsp60 and Hsp70 levels in the liver of all fish but they were lower in larvae fed the 5% than those fed 15 and 25% BW d(-1). Hsp70 level increased much more than Hsp60 after the heat shock Suggesting that Hsp70 is a more sensitive biomarker under our experimental conditions. (c) 2008 Elsevier B.V. All rights reserved.