Developmental potential of vitrified holstein cattle embryos fertilized in vitro with sex-sorted sperm

In vitro fertilization (IVF) is a feasible way to utilize sex-sorted sperm to produce offspring of a predetermined sex in the livestock industry. The objective of the present study was to examine the effects of various factors on bovine IVF and to systema

Syntheses and structures of Na-2[Zn-II(ida)(2)].7H(2)O and Na-4[Hg-II(nta)(2)].7H(2)O

The title complexes were synthesized and the crystal structures of their salts were determined by single-crystal X-ray structure analyses. Na-2[Zn-I(ida)(2)]. 7H(2)O: Triclinic, P1, a=0.523 4(2) nm, b=0.897 10(10) nm, c=1.069 10(10) nm, alpha=85.910(10)degrees, beta= 76.380(10)degrees, gamma=83.52(2)degrees, V=0.484 2(2) nm(3), Z=1. The complex anion [Zn-I (ida)(2)](2-) has a pseudo-octahedral structure in which the two N atoms: are in a trans configuration. Na-4[Hg-I(nta)(2)]. 7H(2)O: Monoclinic, C-c, a = 1.795 0(4) nm, b = 0.892 9(2)nm, c = 1.575 4(2) nm, beta = 92.78 (3)degrees, V = 2.526 2(9) nm(3), Z = 4. The complex anion [Hg-I (nta)(2)](4-) has a pseudo-bicapped-octahedral structure in which the two N atoms are in a trans configuration.

Na_2[Zn~Ⅱ(ida)_2]·7H_2O和Na_4[Hg~Ⅱ(nta)_2]·7H_2O的合成及结构测定

金属离子ＺｎⅡ和ＨｇⅡ分别是生物体必需的微量元素和对人体有害的毒性金属．如何有效地摄取ＺｎⅡ和排除ＨｇⅡ一直是化学家感兴趣的研究课题．而ＺｎⅡ和ＨｇⅡ能与各种氨基酸形成配合物，由于它们处于同一族，电子结构都是ｄ１０，故其配位结构只取决于金属离子半径的…

金属-氨基多羧酸类配合物分子和晶体结构 Ⅰ.Na_2[Fe~Ⅲ(ida)_2]_2·3H_2O配合物的合成及结构测定

氨基多羧酸类配合物的配位数和结构不但与金属离子的半径、电荷和电子结构有关，而且还与氨基多羧酸类配体的形状和大小有关。本文报道了Ｎａ２［ＦｅⅢ（ｉｄａ）２］２·３Ｈ２Ｏ（ｉｄａ＝亚氨基二乙酸根）配合物的合成并测定了它的分子结构和晶体结构。具体结果如下：单斜晶系，Ｐ２１／ｃ空间群，ａ＝１５．６４８０（１０），ｂ＝１６．７８７０（１０），ｃ＝１０．３４７（２），β＝９０．７９０（１０）°，Ｖ＝２７１７．７（６）３，单位晶胞中的分子数为３，对于４７８９个独立的衍射点，它的Ｒ值为０．０３３３。其中两个配合物离子［ＦｅⅢ（ｉｄａ）２］－都是Ｎ和Ｎ顺位的变形八面体结构。由此可知具有对称电子结构（ｈｉｇｈ－ｓｐｉｎｄ５）的ＦｅⅢ与被看成是ｅｄｔａ（＝乙二胺四乙酸根）去掉了ＣＨ２－ＣＨ２的双ｉｄａ配体形成的配合物只能选择六配位八面体结构。

Investigation on molecular and crystal structures of metal complexes with aminopolycarboxylic acids .1. Synthesis and structure of Na-2[Fe-III(ida)(2)](2)center dot 3H(2)O

The crystal structure of the title complex salt has been determined by single-crystal X-ray structure analysis. The crystal data areas follows; Monoclinic, P2(1)/c, a=15.6480(10)Angstrom, b=16.7870(10)Angstrom, c=10.347(2)Angstrom, beta=90.790(10), V=2717.7(6)Angstrom(3), Z=3, and R=0.0333 for 4789 unique reflections. The complex anion has a pseudo-octahedral structure distorted more than the Cr-III and Co-III analogs, in which each, iminodiacetato ligand (ida(2-)) is coordinated in a facial fashion with the two N atoms in a cis configuration, resulting in an unsym-fac structure.

Genetic diversity of cattle in South China as revealed by blood protein electrophoresis

Genetic variation of 31 blood protein loci in 236 cattle from eight South China populations (including mithan, Bos frontalis) and a Holstein population was investigated by means of horizontal starch gel electrophoresis. Thirteen loci (ALB, CAR, Hb-b, Np, PGM, Amy-I, PEP-B, AKP, 6PGD, Cp, Pa, EsD, and TF) were found to be polymorphic. The comparison of average heterozygosities (H) shows that all the native cattle embrace a rich genetic diversity Our results on protein polymorphism suggest that cattle in China originated mainly from Bos indicus and Bos taurus; Xuwen, Hainan, Wenshan, and Dehong cattle and the Dehong zebu are close to zebu-type cattle, and Diqing and Zhaotong cattle are close to the taurine. The mithan was very different from other native cattle, and we suggest that its origin was complicated and may be influenced by other cattle species.

中国蝽科二新种

本文记述了蝽科二新种, 分别属于珀蝽属(Plautia Stal)和卷蝽属(Paterculus Distant). 文中描述所用长度单位均为ｍｍ。

Electroluminescence from Au/(SiO2/Si/SiO2) nanometer double barrier/p-Si structures and its mechanism

SiO2/Si/SiO2 nanometer double barriers (SSSNDB) with Si layers of twenty-seven different thicknesses in a range of 1-5 nm with an interval of 0.2 nm have been deposited on p-Si substrates using two-target alternative magnetron sputtering. Electroluminescence (EL) from the semitransparent Au film/SSSNDB/p-Si diodes and from a control diode without any Si layer have been observed under forward bias. Each EL spectrum of all these diodes can be fitted by two Gaussian bands with peak energies of 1.82 and 2.25 eV, and full widths at half maximum of 0.38 and 0.69 eV, respectively. It is found that the current, EL peak wavelength and intensities of the two Gaussian bands of the Au/SSSNDB/p-Si structure oscillate synchronously with increasing Si layer thickness with a period corresponding to half a de Broglie wavelength of the carriers. The experimental results strongly indicate that the EL originates mainly from two types of luminescence centres with energies of 1.82 and 2.25 eV in the SiO2 barriers, rather than from the nanometer Si well in the SSSNDB. The EL mechanism is discussed in detail.

Solitary magnon excitations in a one-dimensional antiferromagnet with Dzyaloshinsky-Moriya interactions

We investigate solitary excitations in a model of a one-dimensional antiferromagnet including a single-ion anisotropy and a Dzyaloshinsky-Moriya antisymmetric exchange interaction term. We employ the Holstein-Primakoff transformation, the coherent state ansatz and the time variational principle. We obtain two partial differential equations of motion by using the method of multiple scales and applying perturbation theory. By so doing, we show that the motion of the coherent amplitude must satisfy the nonlinear Schrodinger equation. We give the single-soliton solution.

Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry

Peptide mass mapping analysis, utilizing a regenerable enzyme microreactor with metal-ion chelated adsorption of enzyme, combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was developed. Different procedures from the conventional approaches were adopted to immobilize the chelator onto the silica supports, that is, the metal chelating agent of iminodiacetic acid (IDA) was reacted with glycidoxypropyltrimethoxysilane (GLYMO) before its immobilization onto the inner wall of the fused-silica capillary pretreated with NH4HF2. The metal ion of copper and subsequently enzyme was specifically adsorbed onto the surface to form the immobilized enzyme capillary microreactor, which was combined with MALDI-TOF-MS to apply for the mass mapping analysis of nL amounts of protein samples. The results revealed that the peptide mapping could routinely be generated from 0.5 pmol protein sample in 15 min at 50degreesC, even 20 fmol cytochrome c could be well digested and detected.

电子反常供需型Diels-Alder反应合成2-吡啶基吡啶

电子反常供需型Diels-Alder反应(inverse electron demabd Diels-Alder reaction,IDA)因其在天然生物碱及类似物合成中的应用,在近20年来已经逐渐引起关注[1].含氮芳杂环体系1,2,4-三嗪由于其特有的缺电子特征,作为双烯体非常适于进行这类反应[2].本文利用IDA反应从3-吡啶基-5,6-二取代-1,2,4-三嗪出发,设计出一条简捷、低成本的合成路线,成功地合成出不对称取代的2,3′-联吡啶和2,4′-联吡啶.这一类化合物已被应用于药物中间体或有机配体.