NONINVASIVE GIANT PANDA PATERNITY EXCLUSION

Giant panda hair samples obtained by noninvasive methods served as a source of DNA for amplification of seven giant panda microsatellite loci utilizing the polymerase chain reaction. Thirteen giant pandas held in Chinese zoos were tested for identification of paternity. Some males listed as sires have been excluded as the biological father of captive-born giant pandas. Because of the death of some potential sires, paternity is still not assigned for some giant pandas, although there is a high likelihood that paternity assignment could be made if postmortem samples are available for genetic analysis. The DNA microsatellite variation assayed by the test we have developed provides a rapid, highly informative, and noninvasive method for paternity identification in giant pandas. (C) 1994 Wiley-Liss, Inc.

Genetic Stability of Progeny from an Artificial Allotetraploid Carp Using Sperm from Five Fish Species

Microsatellite markers and D-loop sequences of mtDNA from a female allotetraploid parent carp and her progenies of generations 1 and 2 induced by sperm of five distant fish species were analyzed. Eleven microsatellite markers were used to identify 48 alleles from the allotetraploid female. The same number of alleles (48) appeared in the first and second generations of the gynogenetic offspring, regardless of the source of the sperm used as an activator. The mtDNA D-loop analysis was performed on the female tetraploid parent, 25 gynogenetic offspring, and 5 sperm-donor species. Fourteen variable sites from the 1,018 bp sequences were observed in the offspring as compared to the female tetraploid parent. Results from D-loop sequence and microsatellite marker analysis showed exclusive maternal transmission, and no genetic information was derived from the father. Our study suggests that progenies of artificial tetraploid carp are genetically stable, which is important for genetic breeding of this tetraploid fish.

我国部分地区中学生的学校回避倾向研究

The aim of this research is to explore the relationships between school avoidance tendency and school stressors, stress response, social support, coping style of secondary school students. Three studies were carried out. School avoidance tendency scale, mental health scale, coping style scale of secondary school students from Japan were revised in the first research. In the second research, the difference in gender, cities, grades of five variables were discussed. In the third research, the relationships of five variables were discussed. A total of 883 students from grade one of middle school to grade 3 of high school in Beijing, Taiyuan and Wulanchabu, completed three scales. Analysis showed that: 1. The main effects of city, gender, and grade were present significantly on School avoidance tendency, and the inter-effects were not significant. The differences were significant between three grades of middle schools, and not significant between three grades of high schools. The high school students were significant than middle school students on school avoidance tendency. 2. The main effect of city was not significant, but gender, and grade were present significantly on stressor, and the inter-effects were not significant. The most stressor was study one, than the relationship stressor from teachers. The relationship stressor from friends was at least. 3. The main effects of city and grade were present significantly on stress responses, but the main effect of gender was not significant, and the inter-effect was not significant. The most stress response was the physical response. 4. The main effects of city and gender, the inter-effect were not present significantly on social supports. The main effect of grade was present significantly. The most social support was from mother, than from friends, father, and the teachers. 5. The differences between cites on coping styles were not significant. Positive coping style was used mostly, than the style of help requirement, cognitive coping style. 6. Stressor and stress responses had significant positive predictive role on school avoidance tendency. Social supports had negative predictive role. Social supports had not moderator role between stressor, stress responses and schools avoidance tendency. Stress responses had a part of mediator between stressor and school avoidance tendency. 7. In the coping styles, positive coping style and help requirement style had negative predictive role, but cognitive coping style had positive predictive role on school avoidance tendency. Coping styles had negative predictive role. Coping styles had not moderator role between stressor, stress responses and school avoidance tendency.

中学生自我状态现状及其与亲子沟通、心理健康的关系研究

The communication between parents and children is one of the focuses in adolescence research area. However，the ego states in communication were seldom referred to. In present research, the relationship among high school students’ ego states, parent-child communication status and mental health level was explored. Study1: Ego State Questionnaire (ESQ) was revised according to 400 high school students from both rural and urban areas. Study2: Revised Ego State Questionnaire was administered to 450 high school students. Data was analyzed by cluster analysis. Study3: The relationship among ego states, parent-child communication status and mental health level was researched systematically according to 450 high school students. The main results of the thesis were as follows: 1) The five factors of Johu Dusy’s ego states were confirmed. 2) The revised RESQ had good reliability and validity, and could be used in researching middle school students ego states and related area. 3) Gender differences: Girls’ NP score was higher than boys’. Boys’ Adult and AC score was higher than girls. 4)School difference: students’ A and FC from key high school scored higher than students form normal high school. 5) The students were divided into two groups according to parental education level. The split point was high school. Students whose parents’ education level was above high school scored higher in NP than the other group of students. 6) The students were clustered into 4 groups according to cluster analysis: adapted、rational、contradictory、self-centered。 7) adapted、self-centered students’SCL-90 score was lower than average level, contradictory students’ score was higher than average level, rational students’score was equal to average level. 8) Both NP and Adult ego states had significant negative correlation with SCL total score, CP ego states had significant postive correlation SCL total score. 9) NP, A ego states had significant postive correlation with family communication atmosphere, father-son, father-daughter, mother-son, mother-daughter communication status . CP had significant negative correlation with above variables. AC had significant negative correlation with family communication atmosphere . 10) The stepwise regression showed that Adult ，Control Parent ego states and communication between parents and children score had prevented from mental health significantly. Adult ego states impacted mental health through communication between parents and children.

亲子依恋对大学生人际关系影响的性别效应

Parent-child attachment refers to the emotional bond that forms between parent and child, which has great influences on the child’s interpersonal development. Present study applied both correlation method and secure attachment memory activation method to investigate the “gender relation effects” of parent-child attachment’s influences on college students’ close relationships, general attachment representation, and emotion and social loneliness. The basic hypotheses on “gender relation” were set as: “same-sex” parent-child attachment has more influences on college students’ “same-sex” interpersonal relationships, while “opposite-sex” parent-child attachment has more influences on college students’ “opposite-sex” interpersonal relationships. Major findings includes: 1. There was weak correlation of attachment security between father-child and mother-child relationships. The findings indicated that, among college students, the security of “opposite-sex” parent-child attachment representation is higher to some extent than that of “same-sex” parent-child attachment representation. 2. There were significant correlations between parent-child attachment and college students’ attachment anxiety in close relationships. Major findings indicated that “opposite-sex” parent-child attachment security negatively predicts college students’ attachment anxiety in both “same-sex” and “opposite-sex” close relationships. 3. Gender relation effects were significant in the correlations between parent-child attachment and college students’ level of avoidant attachment representation. “Same-sex” parent-child attachment security positively predicted avoidant attachment level in college students’ “same-sex” close relationships, while “opposite-sex” parent-child attachment security positively predicted avoidant attachment level in “opposite-sex” close relationships. 4. Parent-child attachment security memory activation had significant influences on college students’ general attachment representation, in which gender relation effects indicated that: the memory activation of father-child attachment security significantly increases participants’ security of self-model in general attachment representation to “male others”; while the memory activation of mother-child attachment security significantly increase participants’ security of others-model in general attachment representation to “female others”. 5. For male college students, father-son attachment security negatively predicted their emotion and social loneliness. For female college students, father-daughter attachment security negatively predicted their emotion loneliness, while mother-daughter attachment security negatively predicted their social loneliness. Attachment security memory activation had significant influences on college students’ social loneliness, in which gender relation effects was confirmed in that only father-child attachment security memory activation significantly decreased male participants’ level of social loneliness. The results indicated that gender relation effects are significant in the influences of parent-child attachment on college students’ interpersonal relationship representations, especially when the level of avoidant of attachment in college students’ close relationships was predicted by parent-child attachment representation, and when the memory activation of parent-child attachment influenced college students’ general attachment representation. The present study confirmed to some extent that gender relation consistency exists in attachment representations among different interpersonal relations, and serves as a new model for analysis of gender differences in the research fields. In the present study, however, gender relation effects were not confirmed in all the interpersonal relationship representations, which indicated the complexity in the problems of gender differences in the research fields of close relationships.