肝素钠的生产及其存在的问题和解决的方法(II)

就粗品肝素钠生产的原料控制、硬件设施、管理和环保等方面进行了论述，介绍了一些改进的方法和措施，并就该方面的一些问题进行了探讨，提出了解决的方法。

面包质地品质性状的研究II面包小麦烘烤品质的逐步回归分析

测定面包小麦品种的常用品质性状 ,经过选择后 ,确定其蛋白质含量、湿面筋含量、SDS沉降值、Zeleny沉降值、Pelshenke值、溶涨值、和面仪曲线和粉质仪曲线特征值及种子贮藏蛋白高分子量麦谷蛋白亚基评分等为研究对象。结果表明 ,同美国优质面包小麦相比 ,首届面包小麦品种的蛋白质含量和沉降值较高 ;面包体积较大 ,但是面包质地品质 (三项品质评分 :平滑度、弹揉性和内部结构 )表现较差 ;研究的 14个品质特征值变幅较大 ,研究材料表现出不同的蛋白质含量和不同的面筋强度等特点 ,通过逐步回归分析 ,对面包体积影响突出的性状是SDS沉降值、粉质仪形成时间、高分子量麦谷蛋白亚基评分和Pelshenke值 ;对面包的质地品质贡献突出的性状是Zeleny沉降值、和面曲线的和面时间、粉质仪的形成时间和Pelshenke值

Isolation and characterization of photosystem II of Porphyra yezoensis ueda

The thylakoid membranes were isolated and purified from gametophyte of Porphyrayezoensis Ueda (P yezoensis) by sucrose density gradient ultracentrifugation. After R yezoensis gametophyte thylakoid membranes were solubilized with SDS, the photosystem 11 (PSII) particles were isolated and purified. The activity of PSII particles was determined with DCIP (2,6-dichloroindophenol) photoreduction reaction. The composition of purified PSII particles was detected by SDS-PAGE. As a result, seven proteins including 55 kD protein, 47 kD protein, 43 kD protein, 33 kD protein, 31 kD protein, 29 kD protein, and 18 kD protein were found. Compared with PSII particles of higher plants and other algae, they were identified as D1/D2 complex, CP47, CP43, 33 kD protein, D1, D2 and cyt c-550 respectively. Besides, other three new proteins of 20 kD, 16 kD and 14 kD respectively were found. Among these extrinsic proteins, the 16 kD and 14 kD proteins had not been reported previously, and the 20 kD protein was found for the first time in multicellular red algae.

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

Thylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the photosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC. The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein was also detected. However, cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.

Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea

The highly pure and active photosystem II (PSII) complex was isolated from Bangia fusco-purpurea (Dillw) Lyngb., an important economic red alga in China, through two steps of sucrose density gradient ultracentrifugation and characterized by the room absorption and fluorescence emission spectra, DCIP (2,6-dichloroindophenol) reduction, and oxygen evolution rates. The PSII complex from B. fusco-purpurea had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Moreover, the acquired PSII complex displayed high oxygen evolution (139 mu mol O-2/(mg Chl h) in the presence of 2.5 mM 2,6-dimethybenzoqinone as an artificial acceptor and was active in photoreduction of DCIP (2,6-dichloroindophenol) by DPC (1,5-diphenylcarbazide) at 163 U/(mg Chl a h). SDS-PAGE also suggested that the purified PSII complex contained four intrinsic proteins (D1, D2, CP43, and CP47) and four extrinsic proteins (33-kD protein, 20-kD protein, cyt c-550, and 14-kD protein).