重组竹叶青蛇毒纤溶酶原激活剂TSV-PA效应观察体内溶栓

】目的观察重组竹叶青蛇毒纤溶酶原激活剂一体内溶栓效应。方法利用家兔制备的脑血检 模型, 以生理盐水和尿激酶为对照, 用数字减影血管造影技术观察重组竹叶青蛇毒纤溶酶原激活剂飞体内溶 检效果。结果昭高剂量实验组喇再通时间为以内月州低剂量实验组止以内再 通。尿激酶组以刃再通时间为一以内生理盐水对照组没有观察到再通现象。实验动物后处 死解剖, 未观察到出血现象和其它异常现象。结论重组巧勺一在体内具有良好的溶检活性。

B itte r an d Sw e e t /Um am i Ta s te Re c ep to rs w ith D iffe ren tly Evo lu tio n a ry Pa thw ays

通过生物信息学和系统发育学分析,研究了苦味受体和甜味/鲜味受体的进化途径。结果显示,苦味受体 和甜味/鲜味受体在进化上具有远相关,并且具有不同的进化途径,提示这可能是导致这些受体具有不同功能,传 导不同味觉的原因。

昆明水源水和自来水水质致突变性及化学背景值Ⅱ．蝌蚪红细胞微核和CHO细胞染色体畸变及SCE试验

该文研究的目的是以蝌蚪微核及 CHO 培养细胞染色体畸变(CA)及姐妹染色单体交换(SCE)为指示器, 研究昆明市各水厂自来水和它们源水的潜在致突变性. 结果表明：①各水厂自来水及它们的源水诱发蝌蚪微核率, 除Ｙ－水厂自来水外都有显著性差异. ②CA 率的结果: 各水厂自来水及源水和对照组除了Ｗ－水厂自来水外, 都没有显著性差异. ③SCE率: 高浓度组(0.0625L/ml)的滇池源水及Ｗ－水厂自来水和低浓度组(0.03125L/ml)的Ｗ－水厂自来水同对照组相比, 有极显著性差异. 低浓度组滇池源水和高浓度组的Ｅ-, Ｓ-水厂自来水有显著性差异。

麻疹疫苗诱发中国仓鼠卵巢细胞(CHO-K_(1))染色体畸变和基因突变的研 究

以中国仓鼠卵巢细胞(CHO)次黄嘌呤-乌嘌呤磷酸核糖转移酶位点突变检测系统(CHO/HGPRT)检测国产麻疹疫苗的诱变性。实验证明,麻疹病毒及其疫苗可诱发染色体畸变。接种的三个剂量, 对细胞存活率无显著影响(P>0.05),但能使HGPRT位点正向突变频率升高。2.8logTC1D_(50)/ml和3.1logTC1D_(50)/ml组的突变频率的升高与对照组相比有统计学意义。表明HGPRT~(-)突变频率的升高与接种疫苗有关。

微生物降解邻苯二甲酸(O-PA)的动力学

<正> 苯二甲酸及其脂类在石油化工,特别是作为合成工业的增塑剂和韧化剂和在染料制造业中的重要性以及他们对江河湖海的广泛污染而越来越引起人们的关注。Kluwe研究了邻苯二甲酸脂的致癌影响。Kozumbo发现了这种脂类的致突变作用。Kaneshima发现,苯二甲酸浓度在500—1000ppm时毒性甚大;Wilson指出,苯二甲酸钠在500ppm以上时有很大毒性。所以,关于苯二甲酸在天然环境里归趋的研究便相继进行。Ribbons等用测压技术研究了土壤假单胞菌类对苯二甲酸的氧化代谢,证明4,5-二羟基苯二甲酸为其

Effects of solar UV radiation on morphology and photosynthesis of filamentous cyanobacterium Arthrospira platensis

To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which had been grown under sunlight for decades) while they were cultured with three solar radiation treatments: PAB (photosynthetically active radiation [PAR] plus UVR; 280 to 700 nm), PA (PAR plus UV-A; 320 to 700 nm), and P (PAR only; 400 to 700 nm). Solar UVR broke the spiral filaments of A. platensis exposed to full solar radiation in short-term low-cell-density cultures. This breakage was observed after 2 h for the indoor strain but after 4 to 6 h for the outdoor strain. Filament breakage also occurred in the cultures exposed to PAR alone; however, the extent of breakage was less than that observed for filaments exposed to full solar radiation. The spiral filaments broke and compressed when high-cell-density cultures were exposed to full solar radiation during long-term experiments. When UV-B was screened off, the filaments initially broke, but they elongated and became loosely arranged later (i.e., there were fewer spirals per unit of filament length). When UVR was filtered out, the spiral structure hardly broke or became looser. Photosynthetic 0, evolution in the presence of UVR was significantly suppressed in the indoor strain compared to the outdoor strain. UVR-induced inhibition increased with exposure time, and it was significantly lower in the outdoor strain. The concentration of UV-absorbing compounds was low in both strains, and there was no significant change in the amount regardless of the radiation treatment, suggesting that these compounds were not effectively used as protection against solar UVR. Self-shading, on the other hand, produced by compression of the spirals over adaptive time scales, seems to play an important role in protecting this species against deleterious UVR. Our findings suggest that the increase in UV-B irradiance due to ozone depletion not only might affect photosynthesis but also might alter the morphological development of filamentous cyanobacteria during acclimation or over adaptive time scales.

~(239)Pa的鉴别及其特性

利用60 MeV/u18O离子束轰击天然铀靶,经多核子转移反应生成重丰中子核素239Pa。用放射化学方法从被照射的靶中分离出镤。借助于239Pa和它的子体239U的γ射线观测和分析,鉴别了239Pa,测得239Pa的半衰期为(106±30)min。长寿命同位素,如232Th和238U,可给出一个测定核合成持续时间的可能性,而中等寿命同位素,如235U,则可给出有关产生函数时间历史的信息。总地说来,通过232Th/238U2、35U/238U或244Pu/238U的比率,用公式NA(Δ)/NB(Δ)=(PA/PB)f(λA,λB,S0,λR,Δ)便可推导出宇宙年龄。由239Pa的衰变途径,可简单叙述239Pa的异常长的半衰期对于宇宙年龄估计的影响。

用二异丙酮溶剂萃取~(233)Pa(Ⅴ)

用233Pa作示踪剂,苯作稀释剂,研究了二异丙酮(Di-iso-propyl-ketone)对Pa的溶剂萃取行为。分析了萃取效率与震荡时间、不同种类的无机酸浓度和萃取剂浓度的关系及F-对Pa溶剂萃取的影响。结果表明,二异丙酮是萃取Pa的一种优良的萃取剂;二异丙酮-盐酸体系适用于Pa的萃取研究。

甲基异丁基酮萃取Pa(Ⅴ)的研究

为了快速萃取分离Pa,并了解Pa在水溶液中的化学性质,以甲基异丁基酮(MIBK)为萃取剂,苯为稀释剂,以233Pa为示踪剂,研究了盐酸溶液中Pa(Ⅴ)的萃取行为,讨论了萃取时间、盐酸浓度、萃取剂浓度和HF对萃取Pa(Ⅴ)的影响。结果表明,萃取在10 s内达到平衡,甲基异丁基酮能够从大于8 mol/L的HCl溶液中定量萃取Pa,同时HCl介质中加入少量的HF会严重改变Pa的萃取行为。