Flower color chimera and abnormal leaf mutants induced by C-12(6+) heavy ions in Salvia splendens Ker-Gawl.

The effect of C-12(6+) heavy ions bombardment on mutagenesis in Salvia splendens Ker-Gawl. was studied. Dose-response studies indicated that there was a peak of malformation frequency of S. splendens at 200 Gy. Abnormal leaf mutants of the bileaf, trileaf and tetraleaf conglutination were selected. Meanwhile, a bicolor flower chimera with dark red and fresh red flower was isolated in M1 generation of S. splendens. Random amplified polymorphic DNA (RAPD) analysis demonstrated that DNA variations existed among the wild-type, fresh and dark red flower shoots of the chimera. The dark red flower shoots of the chimera were conserved and cultivated at a large-scale through micropropagation. MS supplemented with 2.0 mg/L BA and 0.3 mg/L NAA was the optimal medium in which the maximum proliferation ratio (5.2-fold) and rooting rate (88%) were achieved after 6 weeks. Our findings provide an important method to improve the ornamental quality of S. splendens.

川西地区几种优势苔藓植株元素含量及其时空差异性

揭示苔藓植株元素含量及其时空差异性是深入开展利用苔藓植物指示环境和筛选理想监测藓种的科学基础。采用微波消解（ICP-AES）方法对九寨沟自然遗产地原始林景点停车场周围三个采样带：I为停车场植被小岛（PS），向外约120 m半径为II带（D120），再向外距离约1,000 m为III带（D1000）和3个方向上的大羽藓和毛尖青藓2种苔藓中13种元素（A1, S, Ca, P, Cu, Fe, K, Mg, Mn, Pb, Ni,Zn和Cd）含量进行了测定和比较，并对采自边坡方向（PS-3）上2005和2007年的4种苔藓植物（南木藓、大羽藓、平藓和厚角絹藓）也进行了13种元素含量的测定和比较。同时对九寨沟自然遗产地、黄龙自然遗产地、夹金山和梦笔山的冷杉原始林4个地区的3种苔藓中（大羽藓、锦丝藓和塔藓）的A1、Ca、Cu、Fe、K、Mg、P、Zn、C和N 10种元素含量进行测定和比较。 对大羽藓和毛尖青藓2种苔藓植物内13种元素的测定发现：1）同种苔藓对不同元素的富集能力不同。A1、Ca、K、Mg和P的元素含量在苔藓中的富集较其他元素的要高。2）不同种苔藓植物对同种元素的富集能力不同，毛尖青藓较大羽藓能够监测出更多的元素；对于被检测出的元素，毛尖青藓监测出的累积量大于大羽藓，其中累积量最大的元素是Ca为10 874.286 μg.g-1.year-1，最小的是Cu元素为5.438 μg.g-1.year-1。对05年和07年的平藓、大羽藓、南木藓和厚角絹藓元素含量的测定表明：元素在两年中的积累量是不同的。综合分析表明，苔藓生物监测方法可有效监测景点停车场机动车尾气中排放出的典型元素（如Cu、Pb、Ni、Zn、Cd等）含量，而毛尖青藓较大羽藓能更可靠的监测九寨沟自然遗产地单景点汽车尾气金属元素种类及其排放量。 对4个地区3种苔藓10种元素监测发现：1）Ca、Mg、Zn、K和Cu元素在黄龙地区的含量高于在其它地区的含量；P元素在梦笔山地区的含量为最高；Fe和Al元素在夹金山地区的含量高于在其它地区的含量。2）C元素在其它因素一致的情况下，进行不同年龄间的元素含量比较，结果显示元素含量在各年龄间并不存在明显的差异性；N元素含量与年龄的差异出现在黄龙林下及林窗的塔藓、九寨沟林下的锦丝藓及塔藓和梦笔山的塔藓中，而在锦丝藓中未出现元素含量与年龄的差异性；塔藓能检测出更多的元素种类其含量与年龄间存在显著差异，锦丝藓检测到的元素种类次之；除锦丝藓（锦丝藓在林窗中几乎检测不到元素含量与年龄间的差异性）外，其他两种藓在林窗中能检测出的元素种类大于在林下的检测种类。3）元素含量与生境间存在差异性的元素分别有：黄龙大羽藓中的Ca、P和Mg元素；九寨锦丝藓中的Zn元素；夹金山塔藓中的Al、Fe和Zn元素；梦笔山大羽藓中的Al、Fe和Mg元素及锦丝藓中的Ca元素。4）区域、年龄和生境因素对苔藓植株元素含量均达到了显著水平，但两两之间及三者之间的交互作用由于元素种类的不同存在差异。综合分析表明，苔藓植物中的元素含量受年龄、区域的影响较生境的更大。 The revelation of mosses elements content and its spatiotemporal differences is the scientific foundation of moss monitoring. To determine the feasibility of moss monitoring metal depositions derived from travel bus emiss ions in scenic spot, we collected one year-growth samples of two mosses Brachythecim piligerum and Thuidium cymbifolium with different distance (island center, 120m，1000m ) and different direction (north, south and east) far from parking site island from Yuanshilin Spot at Jiuzhaigou World Nature Heritage in the Western Sichuan of China and determined thirteen element （A1, S, Ca, P, Cu, Fe, K, Mg, Mn, Pb, Ni, Zn, Cd）contents by ICP-AES analysis method. And picked 4 kinds of mosses (Macrothamnium macrocarpu m（Reinw. etHornsch.）Fleisch.，Thuidium cymbifolium，Entodon concinnus (De Not.) Par., Neckera pennata) from the direction PS-3 in 2005 and 2007, determined 13 element contents. Collected 3 kinds of moss (Thuidium cymbifo lium, Actinothuidium Hookeri (Mitt.) and Hyolcomium splendens (Hedw.) from Jiuzhaigou natural heritage, Huanglong natural heritage, Mt. Jiajin and Mt. Mengbi in primeval forest with two habitat (undergrowth and forest gap), determined element contents of A1, Ca, Cu, Fe, K, Mg, P, Zn, C and N. We found that, 1) The elements content are difference in same type of moss. Content of A1, Ca, K, Mg and P are higher than others. 2) While the contents of A1, S, Ca, P, Cu, Fe, K and Zn had significant correlation with the different distances from parking site, the nearer the distance was, the more accumulation was; 2) Comparing to Thuidium cymbifolium, Brachythecium piligerum can test more elements which contents show the significant correlation with distances. And Brachythecium piligerum can have greater element accumulation than Thuidium cymbifolium. The element contents in two years (2005 and 2007) are different. The present study found that moss can reliably bio-indicate metal deposition from traffic emissions in one scenic spot and Brachythecium piligerum is a good moss for bio-indicating element content from traffic emissions at Jiuzhaigou World natural Heritage. Determined the 10 element contents in 4 areas through 3 kinds of moss we found that, 1) Ca, Mg, Zn, K and Cu element content is higher in the Huanglong area than in other areas, The P element content in Mt. Jiajin is higher than other areas, Fe and Al element content is higher in the Mt. Jiajin than in other areas. 2) The content of C had no significant correlation with age. As for N, this significant correlation found in Hyolcomium splendens (Hedw.) with 2 habitat in Huanglong, Actinothuidium Hookeri (Mitt.) and Hyolcomium splendens (Hedw.) with undergrowth in Jiuzhai, Hyolcomium splendens (Hedw.) in Mt. Mengbi. The types of elements which content showt he significant correlation are most in Hyolcomium splendens (Hedw.) and least in Thuidium cymbifolium. Except Actinothuidium Hookeri (Mitt.), the types of elements that content had significant correlation with age in forest gap are more than in undergrowth. 3) The elements which content had significant correlation with habitat are P, Ca and Mg in Thuidium cymbifolium in Huanglong, Zn in Actinothuidium Hookeri (Mitt.) in Jiuzhai, Al, Fe and Zn in Hyolcomium splendens (Hedw.) in Mt. Jiajin, in Mt. Mengbi Al, Fe and Mg in Thuidium cymbifolium and Ca in Actinothuidium Hookeri (Mitt.). 4）The region, the age and the habitat factor has coeffect element content. The correlationship between element contents and the age, the region is closer than habitat.

优良水土保持植物——芨芨草

芨芨草 (Achnatherum splendens)又称积极草、席芨草等 ,属禾本科芨芨草属。多分布于我国北方 ,在黄土高原常分布于道路、村庄及沟坡陡坎上 ,多呈零星块状分布 ,集中连片较少。1　生物生态学特性芨芨草为多年生草本植物 ,构成紧密的丘状草丛 ,丛径一般为 0 .5～ 1 .0 m,茎秆直立、坚硬 ,株高 0 .6～ 1 .8m。叶片坚韧、卷折 ,长 0 .3～ 0 .6 m。园锥花序 ,长 0 .3～ 0 .5 cm,小穗长 6～8mm,灰绿带紫色 ,在黄土高原多为 6～ 7月开花 ,8月中旬到 9月上旬种子成熟 ,芒自外稃齿间伸出 ,不扭转 ,易脱落。根粗坚韧 ,外被沙套 ,须根发达 ,成庞大根系 ,入土深达 0 .5～ 1 .5 m,根直径均达 0 .2～ 0 .3cm,根幅在 1 .5～ 2 .0 m,其根上有白色毛状菌根。在沙质棕钙土上 ,芨芨草有明显分蘖节位移现象 ,在放牧过度地段上 ,由于土壤坚实 ,即盐分集中在土壤上层 ,使之毛细管现象加速土壤干燥 ,因此 ,从土壤的剖面观察到残存的大量死根 ,即活根系集中于剖面的上部 ,由于根量的减少 ,引起分蘖数减少 ,如不合理放牧或刈割利用...

芨芨草在黄土边坡治理中的应用效果

针对黄土高原铁路、公路、大坝及农田地埂边坡长期裸露的水土流失与生态环境问题,选择禾本科植物-芨芨草,进行了为期10年的引种、栽培、生理生态特性与水保等方面的综合开发利用研究。结果表明,芨芨草具有耐旱、耐盐碱、耐瘠薄、抗风沙和极强的固土护坡作用,采用的包衣穴播、分根栽植、基茎扦插3大栽培技术,使芨芨草植株在长期干旱裸露的边坡能快速形成密集的地上部分和发达的根系,固结土壤,防止地表径流冲刷土壤,保护了铁路、公路、大坝及农田地埂边坡的生态安全,为我国西部同类型区的边坡治理,提供了抗逆性较强的优良禾本科植物与配套的栽培技术体系。

青海湖地区围栏封育芨芨草种子形态及其萌发特性

以青海湖地区三角城种羊场的芨芨草(Achnatherum splendens)为观测对泉,研究围栏内外芨芨草的种子外观特征差异,及其在5,10,15,20,25,30℃时的萌发特性,以期评价围栏封育对种子形态和萌发特性的影响.结果表明,围栏内芨芨草种子无论是颗粒大小,还是饱满程度均好于围栏外;在温度低于5℃时,芨芨草种子将不萌发,随着温度的升高,发芽率不断上升,高于25℃发芽率有下降的趋势.低温或高温均不利于种子萌发,即低于20℃或超过25℃萌发均受到不同程度抑制,芨芨草种子萌发适宜温度为20～25℃.围栏内芨芨草种子的萌发状况好于围栏外.