Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis

Capillary zone electrophoresis (CZE) and affinity capillary electrophoresis (ACE) were applied to study the interaction between netropsin and a 14mer double-stranded DNA (dsDNA). The use of a polyacrylamide coated capillary can suppress the electroosmotic flow (EOF) and the adsorption of DNA onto the wall. Better analysis of the DNA was achieved in a coated capillary upon Tris-acetate. In CZE, the peak width broadened due to the affinity interaction between dsDNA and netropsin. In ACE, o-toluic acid, a negatively charged molecule was used as the indicator to monitor the changes of EOF when netropsin was added to the running buffer. The 14mer dsDNA showed different mobilities upon various concentrations of netropsin due to the affinity interaction between the dsDNA and netropsin. The binding constants of this interaction were (1.07 +/- 0.10) . 10(5) M-1 calculated from CZE and (4.75 +/- 0.30) . 10(4) M-1 from ACE using a Scatchard plot. The binding stoichiometry was 1:1 calculated from CZE which was superior to ACE in this study. (C) 2002 Elsevier Science B.V. All rights reserved.

土壤资源信息共享的WEBGIS实现

如何使用和共享现有的土壤科学数据一直是一个重要问题。为提高土壤资源信息的共享和利用效率,文章阐述了在因特网环境下实现土壤资源信息发布的体系结构及关键技术,讨论了其实现方法。采用WebGIS技术,以ARCIMS为平台,以广东省土壤系列图件为基础,构建土壤信息共享系统,通过WEBGIS进行土壤资源信息的发布和共享,并介绍了系统的主要功能。