重组人甲状旁腺素(rhPTH(1-84))的原核表达及发酵条件初步优化

下载PDF阅读器甲状旁腺激素(Parathyroid Hormone,PTH)是治疗骨质疏松症的药物之一.将人工合成全长人PTH(hPTH(1-84))的核苷酸序列插入pThioHis A载体中,然后转化大肠杆菌(Escherichia coli.),在IPTG的诱导下,成功实现了rhPTH(1-84)的原核表达.通过发酵条件的优化,初步确定1:40接种.LB+30% M9盐溶液的发酵培养基,37℃培养至OD600nm=0.8时,加入终浓度为0.6 mmol/L的IPTG,诱导8 h的较优发酵程序.

Utility of ITS1-5.8S-ITS2 sequences for species discrimination and phylogenetic inference of two closely related bucephalid digeneans (Digenea : Bucephalidae): Dollfustrema vaneyi and Dollfustrema hefeiensis

The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from 60 specimens belonging to two closely related bucephalid digeneans (Dollfustrema vaneyi and Dollfustrema hefeiensis) from different localities, hosts, and microhabitat sites were cloned to examine the level of sequence variation and the taxonomic levels to show utility in species identification and phylogeny estimation. Our data show that these molecular markers can help to discriminate the two species, which are morphologically very close and difficult to separate by classical methods. We found 21 haplotypes defined by 44 polymorphic positions in 38 individuals of D. vaneyi, and 16 haplotypes defined by 43 polymorphic positions in 22 individuals of D. hefeiensis. There is no shared haplotypes between the two species. Haplotype rather than nucleotide diversity is similar between the two species. Phylogenetic analyses reveal two robustly supported clades, one corresponding to D. vaneyi and the other corresponding to D. hefeiensis. However, the population structures between the two species seem to be incongruent and show no geographic and host-specific structure among them, further indicating that the two species may have had a more complex evolutionary history than expected.

Intraspecific phylogeography of Carchesium polypinum (Peritrichia, Ciliophora) from China, inferred from 18S-ITS1-5.8S ribosomal DNA

Based on the variation of site 34, 46, 241, 305 and 322 in the 18S-ITS1 rDNA sequence, 19 Carchesium polypinum populations collected from eight provinces of China were separated into northern and southern population along the delineation between the Yangtze River and the Pearl River. This geographic distribution pattern of Carchesium polypinum maybe results from two factors: the vicariance resulting from the formation of the delineation between the Pearl River and the Yangtze River accompanied with the uplift of Qinghai-Xizang Plateau, and the different dispersal paths of C. polypinum affected by the climate.

温度引起的ZnSe/Zn_（0.84）Mn_（0.16）Se超晶格中势阱层和势垒层的反转

测量了ZnSe，Zn_(0.84)Mn_(0.16)Se合金和ZnSe/Zn_(0.84)Mn_(0.16)Se超晶格的10～300K的变温光致发光谱。发现ZnSe的带隙在10K时比Zn_(0.84)Mn_(0.16)Se合金的带隙小，而在300K时比合金的带隙大。预计ZnSe/Zn_(0.84)Mn_(0.16)Se超晶格中在130K附近会发生势阱层和势垒层的反转。在ZnSe/Zn_(0.84)Mn_(0.16)Se超晶格中观测到了这种反转但发生在80K附近。超晶格中Zn_(0.84)Mn_(0.16)Se层的应变可能是反转温度变低的原因。

84.5MeV~(12)C~(4+)离子诱发金属靶原子发射的Kβ与Kα-X射线强度比值的研究

本实验是在中国科学院兰州近代物理研究所,国家实验室的重离子加速器上完成的.实验用OR-TEC公司生产的HPGe X射线探测器测量了84.5 MeV的12C4+离子轰击Cu,Mo,Ag,Cd,In,Sn,W和Au金属靶产生的K壳层特征X射线谱,计算了Kβ与Kα-X射线强度的比值,并将结果与Scofield用Hartree-Fock-Slater模型计算出的理论值与用其它方法(如:衰变幅射,用光子,电子,质子等粒子与靶相互作用)得到的实验值进行了比较.比较结果表明用84.5 MeV的12C4+离子轰击Cu,Mo,Ag,Cd,In,Sn,W和Au金属靶产生的Kβ与K-αX射线强度比值比理论值和其它实验值要大许多.

Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis

The sequences of the ITS (internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli (NB, PT and ST) were amplified, sequenced and analyzed. In addition, the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated. The results are as follows: the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains. The sequences of ITS l were 331 by to 334 bp, while those of the 5.8S rDNA were 158 by and the sequences of ITS2 ranged from 673 by to 681 bp. The sequences of the ITS had a high level of homology (up to 99.5%) with that of P. haitanensis (DQ662228) retrieved from GenBank, but were only approximately 50% homologous with those of other species of Porphyra. The results obtained when a phylogenetic tree was constructed coincided with the results of the homology analysis. These results suggest that the three cultivated strains of P. haitanensis evolved conservatively and that the ITS showed evolutionary consistency. However, the sequences of the ITS and 5.8S rDNA of different Porphyra species showed great variations. Therefore, the relationship of Porphyra interspecies phyletic evolution could be judged, which provides the proof for Porphyra identification study. However, proper classifications of the subspecies and the populations of Porphyra should be determined through the use of other molecular techniques to determine the genetic variability and rational phylogenetic relationships.