大模场面积光纤激光器79．4W单横模输出

abstract {A large-mode-area (LMA) multimode fiber before and after coiling was studied contrastively in the experiment. Single-transverse-mode output was achieved when the fiber laser was coiled around a mandrel of 65 mm radius. After coiling, beam quality factor of the laser dropped from 1.24 to 1.06 and slope efficiency dropped from 64.7% to 54.3%. When the launched pump power was 149 W, the corresponding output power was 94.7 W and 79.4 W, respectively. However, the brightness of the coiled fiber laser was 1.15 times that of the uncoiled. Coiled modal losses of different modes were also calculated for the fiber employed in the experiment. The measured results agree well with the calculated ones.}

昆明山海棠对中国仓鼠V_(79)细胞二酰基甘油含量的影响

研究以昆明山海棠根部水抽提物(Tripterygium hypoglaucum (Level) Hutch,THH)处理中国仓鼠V_(79)细胞,通过检测V_(79)细胞C-M细胞频率以及二酰基甘油(1,2-diacylgcerol,DAG)的含量测定,分析了THH诱发非整倍体与细胞醇磷酯信号通路的关系。结果指出:THH能在1mg/ml、2mg/ml两个剂量上使V_(79)细胞的DAG含量显著升高(P<0.001),并明显的提高C-M细胞频率(P<0.05),提示肌醇酯信号通路是介导THH诱发非整倍体的途径之一。

Construction of AFLP-based genetic linkage map for Zhikong scallop, Chlamys farreri Jones et Preston and mapping of sex-linked markers

Zhikong scallop (Chlamys farreri) is an economically important aquaculture species in China; however, frequent mass mortality seriously affects the development of its industry. Genetic linkage map is useful for genetic improvement and selective breeding of C. farreri. Linkage maps were constructed using an intraspecific F-1 cross and amplified fragment length polymorphism (AFLP) markers. Thirty-two selected AFLP primer combinations produced 545 AFLP markers that were polymorphic in either of the parents and segregated in the progeny. Of these segregating markers, 166 were mapped to 19 linkage groups of the female framework map, covering a total of 1503.9 cM, with an average marker spacing of 10.2 cM; and 197 markers were assigned to 20 linkage groups of the male map, covering a total of 1630.7 cM, with 9.2 cM per marker. A sex-linked marker was mapped on the female map with zero recombination and a LOD of 27.3. The genetic length of C farreri genome was estimated as 1889.0 cM for the female and 1995.9 cM for the male. The coverage of the framework map was calculated as 79.6% for the female and 81.7% for the male. When the triplets and doublets were considered, the observed length of the map was calculated as 1610.2 cM with coverage of 85.2% for the female, and 1880.5 cM with coverage of 94.2% for the male. The genetic maps presented here will serve as a basis for the construction of a high-resolution genetic map and mapping of economically important genes. (C) 2004 Published by Elsevier B.V.