红壤地区陆气耦合模式研究及其应用

论述了陆气相互作用研究在人类生存环境与发展区域经济中的重要意义与研究现状，在原有研究工作基础上，针对中国科学院红壤试验站地区陆气水热传输过程，提出了一个多层陆气耦合模式。对植被内部湍流交换的物理过程作了深入研究，特别考虑了叶片气孔为非饱和水汽条件下的交换情况，并且给出了修正后的根系吸水模式。陆气耦合模式分别对大气、植被、土壤作多层划分，以助于细致了解沿高度分布的各物理量。利用本模式对中国科学院红壤试验站地区油菜地陆气水热交换过程进行了数值模拟，模拟结果与实测值吻合较好。证明该模式成功地模拟了陆气相互作用过

双模式高精度可调超分辨光瞳滤波器

基于双折射波片的特性，提出了一种具有双调节模式的连续可调超分辨光瞳滤波器。该滤波器通过两种调节模式实现超分辨，且在一定范围内连续可调。在横向旋转模式下，该滤波器为复振幅型光瞳滤波器，纵向倾斜模式下为纯相位型超分辨滤波器。通过对超分辨参量的分析，得到最佳设计参量，可以实现大的视场角，大的超分辨范围、高的调节精度。这种滤波器为实际应用提供了更多的方便。

深海鱼皮胶原肽紧肤抗衰老面膜及制备方法

本发明公开一种深海鱼皮胶原肽紧肤抗衰老膏状面膜及其制备方法。该方法以提取的深海鱼皮胶原多肽1~10份结合玫瑰纯露1~3份、海藻糖1~2份、菊糖0.5~1份为功能原料，以无机粉状物10~30份、淀粉5~10份、保湿剂5~10份、增稠剂1~2份、油脂5~10份以及表面活性剂1~2份为辅助原料，原料中各组分均以重量计，经溶解、混合、搅拌、均质等工艺制得深海鱼皮胶原肽紧肤抗衰老膏状面膜。本发明的胶原多肽面膜主要以天然动植物提取物为活性成分，不仅具有深层清洁、补充肌肤营养、平整细纹、延缓衰老的功能，而且具有良好的延展性和粘接性，是一种经济实用有效的美容护肤化妆品。

Polymerase chain reaction based C4AQ0 and C4BQ0 genotyping: association with systemic lupus erythematosus in southwest Han Chinese

Objective: To investigate the association of complement C4 null genes (C4QO, including C4AQO and C4BQO) and C2 gene with systemic lupus erythematosus (SLE) in southwest Han Chinese; 136 patients with SLE and 174 matched controls were genotyped. Methods: C4 null genes were determined by a polymerase chain reaction (PCR) procedure with sequence specific primers (PCR-SSP). The 2 bp insertion in exon 29, which was previously identified in non-Chinese populations and caused defective C4A genes, was directly typed by sequencing the whole exon 29 using exon specific primers. The exon 6 of complement C2 was also sequenced in both the patients and controls. Results: The frequency of homozygous C4AQO allele was 12.5% (17/136) in patients with SLE compared with 1.1% (2/174) in controls (p<0.001, odds ratio (OR)=12.286, 95% confidence interval (95% CI) 2.786 to 54.170). There was no significant difference for homozygous C4BQO allele between patients with SLE and controls (p=0.699). Patients with the C4AQO gene had an increased risk of acquiring renal disorder, serositis, and anti-dsDNA antibodies compared with those without C4AQO (for renal disorder, p=0.018, OR=8.951, 95% Cl 1.132 to 70.804; for serositis, p=0.011, OR 4.891, 95% CI 1.574 to 15.198; for anti-dsDNA, p=0.004, OR 7.630, 95%Cl 1.636 to 35.584). None of the patients or controls had the 2 bp insertion in exon 29 of the C4 gene. The type I C2 deficiency was not detected in the 3 10 samples. Conclusion: It is suggested that deficiency of C4A (not due to a 2 bp insertion in exon 29), but not C4B or C2, may be a risk factor for acquiring SLE in south west Han Chinese; this results in increased risk of renal disorder, serositis, and anti-dsDNA antibodies in patients with SLE. Racial differences seem to be relevant in susceptibility to SLE.

Abnormal regional homogeneity of drug-naive obsessive-compulsive patients

To explore the possible abnormal resting-state activity in patients with obsessive-compulsive disorder (OCD), the regional homogeneity (ReHo) of 22 pairs of patients and well-matched healthy controls was calculated. Compared with controls, the patients showed higher ReHo in the left anterior cingulate cortex, but lower ReHo in the left inferior temporal gyrus. These findings supported the abnormal resting-state brain activity in drug-naive OCD patients. No significant correlations between ReHo value and four clinical characteristics were found, suggesting that abnormal ReHo might be trait-related in OCD. NeuroReport 21:786-790 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

柱状黄杆菌胶原蛋白酶的克隆与表达

利用PCR方法从柱状黄杆菌(Flavobacterium columnare)G4株中克隆了1个胶原蛋白酶基因(GenBank登录号EF501979)。同源性比对发现该基因与柱状黄杆菌的另外1种胶原酶基因(GenBank登录号EAZ95511.1)最为相近,有84%的一致性和93%的相似性。该属的另外2种细菌,约氏黄杆菌(Flavobacterium johnsoniae)和嗜冷黄杆菌(F.psychrophilum)都有与该胶原酶基因相似的基因,其相似性分别为92%和83%。该基因经KpnI和SalI酶

三峡库区藻类种群结构与密度变化及其与氮磷浓度的相关性分析

2003年1月~2004年12月对三峡湖北库区江段5个采样点及其一级支流——香溪河流域6个采样点的浮游藻类进行了为期2周年的调查,共采集和鉴定浮游藻类定性和定量水样1 000余个,获得有效数据2 000多个。数据显示,蓄水前(2003年1月~2003年6月)、蓄水1周年(2003年6月~2004年5月)和蓄水1.5年(2004年6月~2004年12月)3个时间段之间,被调查水域的浮游藻类群落结构、细胞密度和水质状况均存在一定的差异。总体而言,藻类的群落结构干流江段以硅藻为主,绿藻次之;支流水域以绿藻占据首

微囊藻毒素LR诱导细胞毒性机制研究的细胞模型建立

以18种细胞系为材料,研究微囊藻毒素LR(20μg/mL和50μg/mL)所诱导的细胞毒性。形态观察表明,在经过30h以上的微囊藻毒素处理后,PC-3,J82,786-0,5637,VERO-E6等5种细胞出现了明显的细胞形态改变,毒素浓度越高,形态改变越厉害。微囊藻毒素LR的细胞毒性用LDH泄漏来表示。结果显示,5种毒素处理细胞的LDH泄漏呈剂量依赖性增加,其中5637和PC-3的LDH泄漏在同样的处理后较为厉害;同对照比较,SOD活力在20μg/mL MCLR处理下呈增加趋势,但在50μg/mL浓度下

Combined effects of ultraviolet radiation and temperature on morphology, photosynthesis, and DNA of Arthrospira (Spirulina) platensis (Cyanophyta)

Natural levels of solar UVR were shown to break and alter the spiral structure of Arthrospira (Spirulina) platensis (Nordst.) Gomont during winter. However, this phenomenon was not observed during summer at temperatures of similar to 30 degrees C. Since little has been documented on the interactive effects of solar UV radiation (UVR; 280-400 nm) and temperature on cyanobacteria, the morphology, photosynthesis, and DNA damage of A. platensis were examined using two radiation treatments (PAR [400-700 nm] and PAB [PAR + UV-A + UV-B: 280-700]), three temperatures (15, 22, and 30 degrees C), and three biomass concentrations (100, 160, and 240 mg dwt [dry weight] . L-1). UVR caused a breakage of the spiral structure at 15 degrees C and 22 degrees C, but not at 30 degrees C. High PAR levels also induced a significant breakage at 15 degrees C and 22 degrees C, but only at low biomass densities, and to lesser extent when compared with the PAB treatment. A. platensis was able to alter its spiral structure by increasing helix tightness at the highest temperature tested. The photochemical efficiency was depressed to undetectable levels at 15 degrees C but was relatively high at 30 degrees C even under the treatment with UVR in 8 h. At 30 degrees C, UVR led to 93%-97% less DNA damage when compared with 15 degrees C after 8 h of exposure. UV-absorbing compounds were determined as negligible at all light and temperature combinations. The possible mechanisms for the temperature-dependent effects of UVR on this organism are discussed in this paper.