7 resultados para 63, 22 p.

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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Although spermatozoa from several species of nonhuman primates have been cryopreserved, there has been no report of success with rhesus macaque spermatozoa as judged by functional assays. Two Tris-egg yolk freezing media. TEST and TTE. which have: been successfully used for cynomolgus macaque (Macaca fascicularis) spermatozoa, were compared for cryopreservation of spermatozoa From four rhesus macaques (Macaca mulatta). The postthaw motility (percentage and duration) of spermatozoa cryopreserved in TTE was much higher than that for spermatozoa cryopreserved in TEST. The function of sperm cryopreserved in TTE was evaluated by in vitro fertilization or oocytes collected from gonadotropin-stimulated prepubertal rhesus macaques. Of the inseminated oocytes. 82 +/- 13% were fertilized and 63 +/- 22 and 39 +/- 21% of the resulting zygotes developed into morulae and blastocysts. respectively. These results indicate that rhesus macaque spermatozoa can be effectively cryopreserved in TTE medium. This finding will facilitate the application of in vivo and in vitro assisted reproductive technologies in this species. (C) 2001 Academic Press.

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: 4.83%4.95%(P<0.05)26.73%19.89%1.941.56SODACPMDASOD(P<0.05)995.43113.99446.6440.6ACP(P<0.05)90.19432.3673.26146.63MDA(P>0.05)3.3792.462.1728.96 (0, 0.2, 0.3, 0.5 mg L-1) Pb2+Pb2+SODACPMDAPb2+Pb2+Pb2+Pb2+Pb2+SODSODPb2+ACPACPPb2+MDAMDAPb2+Pb2+Pb2+ 1830(P>0.05)Reactive Oxygen SpeciesROSROS(P<0.05) SODACPMDA: 22.8220.96SOD(P<0.05)613.88102.01356.1550.22ACP(P<0.01)44.32.91ACP455.75485.33MDA2.6731.833.1735.33

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(Caragana korshinskii)(Hedysarum laeve)(Artimisia sphaerocephala)(Artimisia ordosica) 4797364 (1) 10-20 mm0.5-2 cm5 cm;10-20 mm0.5 cm1.52 cm0.5 cm4 (2) (30)57.57.5mm;15-20 mm50%;10 mm50%;4 (3) 0.5 cm7.5 mm4 (4) 2-7 cm41 cm57 cm1-5 cm20%7 cm45.23.27%48.45.21%20.84.63%22.44.83%4 (5) 20.250.5 H00.75 HRGRNAR75 mm/100 mm/0.50.75 H;50 mmRGR75 mmRGRNAR

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,X,,,(541.348.8)(4351724)mg/kg,(3.830.73)(4.281.56)mg/L(3.681.05)(0.450.25)mg/L96.15%89.60%25.24%,63.17%,(P<0.01);42.61%,28.83%,(P<0.01),

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WAXDNylon-1010;=4.9(?),b=5.4(?),c=27.8(?),=49,=77,=63.5,,P(?)Ruland60SAXS,Nylon-1010

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208090 2005520075KClTGMRGMTPM2 1 3.941.16 ng m-3 2 6.23.9 pg m-330.732.1 pg m-30.2%0.8% 3 8.63~22.5 ng m-3>4.74 ng m-3>2.55~8.83 ng m-3>1.65~3.57 ng m-3 4 5 >>>>>> 6 160030009.126.1 g m-2 yr-157.0 g m-2 yr-151.9 g m-2 yr-115.9 g m-2 yr-1(31 %) 7 142 kg yr-1>95%