85 resultados para 617 - Cirurgia. Ortopèdia. Oftalmologia

em Chinese Academy of Sciences Institutional Repositories Grid Portal


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In order to investigate the characteristics of water wave induced liquefaction in highly saturated sand in vertical direction, a one-dimensional model of highly saturated sand to water pressure oscillation is presented based oil the two-phase continuous media theory. The development of the effective stresses and the liquefaction thickness are analyzed. It is shown that water pressure oscillating loading affects liquefaction severely and the developing rate of liquefaction increases with the decreasing of the sand strength or the increasing of the loading strength. It is shown also that there is obvious phase lag in the sand Column. If the sand permeability is non-uniform, the pore pressure and the strain rise sharply at which the smallest permeability occurs. This solution may explain Why the fracture occurs in the sand column in some conditions.

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采用原位观测的方法研究了脆性泡沫铝材料在压缩载荷下细观与宏观断裂破坏规律和吸能机理。针对多孔泡沫金属材料提出一种细观原位加载实验方法,采用特别设计与制备的试件,在S570扫描电镜下研究了特定胞孔在压缩过程中孔壁的失效顺序和破坏规律,并揭示了能量吸收的细观机理。对块体材料的宏观压缩实验表明,脆性泡沫铝是以多个断裂带的形式破坏。研究发现,孔壁缺陷和胞孔形态缺陷是诱发断裂带形成与发展的重要因素。依据尺寸效应对细观与宏观实验下泡沫铝的性能进行了比较。

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The ablation in zinc selenide (ZnSe) crystal is studied by using 150-fs, 800-nm laser system. The images of the ablation pit measured by scanning electronic microscope (SEM) show no thermal stress and melting dynamics. The threshold fluence is measured to be 0.7 J/cm2. The ultrafast ablation dynamics is studied by using pump and probe method. The result suggests that optical breakdown and ultrafast melting take place in ZnSe irradiated under femtosecond laser pulses.

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We present a novel phase-shifting vectorial-shearing interferometer with a wedge plate phase-shifter. The interferometer is based on a modified Mach-Zehnder configuration; the common-path nature makes it capable of testing the wavefront of a short coherence-length light source, such as a laser diode. Vectorial shear (shearing in the x and y directions simultaneously) in an arbitrary direction is introduced by inserting two wedge plates orthogonally in two arms, respectively. One of the wedge plates is split into two parts (parallel part and wedge part); phase shift is produced by moving the wedge part in contact along the parallel part. The moving distance for a 2 pi phase shift is a few millimetres in specific conditions. The wedge plate phase-shifter increases the moving distance for phase shift and makes the control of phase shift relatively easy. We also discuss the lateral shear error and phase shift errors induced by wedge plates. The lateral shear error is small enough to be ignored; the phase shift error is determined mainly by the wedge angle error. Lastly, we give the experimental results of phase-shifting interference fringes in vectorial shear mode.

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Spurious reflection is one of the troublesome problems in phase-shifting interferometry. This paper deals with the problem on the basis of a two-run-times-two-frame phase-shift algorithm, in which the phase shifts are shared out between the reference beam and the object beam. The effect of spurious reflection on phase measurement is investigated; two simple methods for removal of the effect are presented and each needs only six interferograms. Two other solutions to the spurious reflection problem are also reviewed. The simulation results obtained using these four solutions are compared. The influence of a mix of phase-shifter miscalibration and spurious reflection on phase measurement is also discussed.

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Dependence of performances of non-line-of-sight (NLOS) solar-blind ultraviolet (UV) communication systems on atmosphere visibility is investigated numerically by correlating the propagation of UV radiation with the visibility. A simplified solar-blind UV atmospheric propagation model is introduced, and the NLOS UV communication system model is constituted based on the single scattering assumption. Using the model, numerical simulation is conducted for two typical geometry configurations and different modulation formats. The results indicate that the performance of the NLOS UV communication system is insensitive to variation of visibility in quite a large range, and deteriorates significantly only in very low-visibility weather, and is also dependent on the geometry configuration of the system. The results also show that the pulse position modulation (PPM) is preferable due to its high-power efficiency to improve the system performance. (c) 2007 Elsevier GmbH. All rights reserved.

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We prepare bismuth-doped borosilicate glasses and the luminescence properties in infrared wavelength region are investigated. Transmission spectrum, fluorescence spectrum and fluorescence decay curve are measured. The glasses exhibit a broad infrared luminescence peaking at 1340nm with the full width at half maximum of about 205nm, and lifetime of 273 mu s when excited by an 808-nm laser diode. The glasses are promising materials for broadband optical amplifiers and tunable lasers.

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花粉管是有花植物受精过程中雄性生殖单位的载体,它同根毛、真菌菌丝一样,具有典型的极性顶端生长模式。裸子植物花粉与被子植物相比,具有萌发时间较长,生长缓慢等特点。但是目前人们对于裸子植物花粉萌发和花粉管生长的机理还不清楚。本文以裸子植物白皮松(Pinus bungeana)的花粉为材料,采用细胞学和生理生化方法,包括应用普通光学显微镜、荧光显微镜、激光扫描共聚焦显微镜、显微红外光谱(FTIR)和透射电镜(TEM)等技术,对其花粉萌发和花粉管生长过程进行了较为系统的研究,旨在进一步揭示裸子植物花粉管发育的调控机制。 本论文首先研究了外源Ca2+ 和3种调钙药物(A23187、EGTA、TMB8)对白皮松花粉萌发和花粉管生长的影响。结果表明,在离体培养条件下,高浓度的Ca2+(1%)能完全抑制白皮松花粉的萌发,低浓度的Ca2+ 则影响不大,而花粉萌发和花粉管生长的最适Ca2+ 浓度为0.01%。用Ca2+ 载体A23187、Ca2+ 螯合剂EGTA和钙通道阻滞剂TMB8分别处理花粉后,花粉萌发和花粉管生长均受到抑制。另外,用 Ca2+ 荧光探针 Fluo-3AM标记,对Ca2+ 的分布变化进行了观察,发现在花粉萌发的初期,Ca2+ 向萌发孔聚集。在正常生长的花粉管中Ca2+ 呈梯度分布,顶端荧光最强。与对照相比,A23187处理后花粉粒中荧光增强,而EGTA和TMB8处理的花粉粒中荧光强度均减弱。并且这3种调钙药物还破坏了花粉管顶端的Ca2+ 浓度梯度,最终导致花粉管的生长受阻。 花粉萌发和花粉管的生长依赖于RNA和蛋白质的不断合成。在放线菌素D的存在下,花粉萌发基本不受影响,但花粉管的生长速度下降,花粉管中RNA含量也减少。而经过放线菌酮处理后,花粉萌发和花粉管生长均受到抑制,花粉管中蛋白质含量降低,同时花粉管顶端显著膨大。通过SDS-PAGE的结果表明,花粉粒萌发前后蛋白质图谱有明显差异。FTIR光谱分析表明,两种抑制剂处理均导致花粉管壁的化学组成发生了变化,例如蛋白质和饱和酯含量减少,而羧酸的含量增加。此外,由放线菌酮和放线菌素D处理后,花粉管的超微结构也发生了明显变化,其中特别是花粉管顶端的分泌系统遭到严重破坏。 纤维素的正常合成对于白皮松花粉管的生长是必需的。在正常培养基中添加纤维素生物合成抑制剂2,6-二氯苯腈(DCB)后,花粉萌发几乎不受影响,但是花粉管的形态发生异常,生长速率降低。DCB处理还导致花粉管壁中纤维素含量下降,而胼胝质在花粉管顶端积累。用识别酯化果胶的JIM7和识别酸性果胶的JIM5对离体培养的白皮松花粉管进行标记后,发现果胶成分呈异常分布图式。FTIR光谱分析结果表明花粉管细胞壁中蛋白质、羧酸以及饱和酯含量增加。同时,在电镜下观察发现,花粉管细胞壁顶端呈现不均匀加厚,其中主要的细胞器,如高尔基体和线粒体等膜结构均遭到破坏。 上述结果说明,白皮松成熟花粉粒中已含有花粉萌发和花粉管早期生长所必需的Ca2+ 和RNA,但是在花粉管的后续伸长过程中仍需要外源Ca2+ 的参与以及新RNA、蛋白质的不断合成。与被子植物不同,裸子植物花粉萌发的启动也需要新蛋白的合成。尽管在花粉管中纤维素的含量很低,但是对于细胞壁的构建、花粉管的正常形态的维持起着关键作用。

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MicroRNAs (miRNAs) are a growing class of small RNAs ( about 22 nt) that play crucial regulatory roles in the genome by targeting mRNAs for cleavage or translational repression. Most of the identified miRNAs are highly conserved among species, indicating

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以冷冻精子的复苏运动度、荧光染料Hoechst 33258 检测的细胞膜完整率、异硫氰酸荧光素标记的 花生凝集素(FITC2PNA) 检测的顶体完整率作为精子功能状态的指标, 对甘油、二甲亚砜、乙二醇和丙二醇4 种常用渗透性防冻剂在猕猴精子冷冻保存过程中的作用进行了比较。结果表明: 冷冻保存精子的复苏运动度, 甘油(4713 ±517 %) 和乙二醇(4418 ±617 %) > 二甲亚砜(2219 ±019 %) > 丙二醇(0 ±0 %) ; 细胞膜完整 率, 甘油(5418 ±312 %) 和乙二醇(5410 ±617 %) > 二甲亚砜(3715 ±710 %) > 丙二醇(2813 ±615 %) ; 顶 体完整率, 甘油(8212 ±214 %) 和乙二醇(8214 ±214 %) > 二甲亚砜(6817 ±517 %) 和丙二醇(7213 ± 315 %) ( P < 0105) 。结果提示: 二甲亚砜和丙二醇, 尤其是丙二醇并不适合猕猴精子的冷冻保存; 而乙二醇具 有和甘油相似的保护作用, 是一种极具潜力的猕猴精子冷冻保存的渗透性防冻剂。

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Bulges are common features of folded RNA structures. The RNA axial kinking caused by bulges has been confirmed by many experiments. Usually, a kinking angle zeta and a bending angle theta are used to describe the kinking and twisting of RNA molecules containing bulges. Here, we present two additional angles (twist angle zeta(1), twist angle zeta(2)) to describe the deformation of RNA helices induced by bulge loops because only two angles (a kinking angle zeta and a bending angle theta) are not enough to define the deformation of RNA induced by bulges. (C) 2002 Elsevier Science B.V. All rights reserved.