Metabolism traits of 'all-fish' growth hormone transgenic common carp (Cyprinus carpio L.)

Transgenic animals with improved qualities have the potential to upset the ecological balance of a natural environment. We investigated metabolic rates of 'all-fish' growth hormone (GH) transgenic common carp under routine conditions and during starvation periods to determine whether energy stores in transgenic fish would deplete faster than controls during natural periods of starvation. Before the oxygen uptake was measured, the mean daily feed intake of transgenic carp was 2.12 times greater than control fish during 4 days of feeding. The average oxygen uptake of GH transgenic fish was 1.32 times greater than control fish within 96 h of starvation, but was not significantly different from controls between 96 and 144 h of starvation. At the same time, GH transgenic fish did not deplete energy reserves at a faster rate than did the controls, as the carcass energy contents of the two groups following a 60-d starvation period were not significantly different. Consequently, we suggest that increased routine oxygen uptake in GH transgenic common carp over that of control fish may be mainly due to the effects of feeding, and not to an increase in basal metabolism. GH transgenic fish are similar to controls in the regulation of metabolism to normally distribute energy reserves during starvation. (c) 2008 Published by Elsevier B.V.

Influence of rapid thermal annealing on InAs/InAlAs/InP quantum wires with different InAs deposited thickness

We have studied the effect of the post-growth rapid thermal annealing on optical and electrical properties of InAs/InAlAs/InP quantum wires with various InAs deposited thickness. Quite different annealing behaviors in photoluminescence and dark resistance are observed, which can be attributed to dislocations in samples. After annealing at 800 degrees C, quantum wires still exist in the sample with two monolayer InAs deposited thickness, but the temperature-dependent PL properties are changed greatly due to the intermixing of In/Al atoms. (c) 2005 Elsevier B.V. All rights reserved.

糖类衍生物在毛细管区带电泳下的分离研究

以新合成的1-萘基-3-甲基-5-吡唑啉酮（NMP）为柱前衍生试剂，采用毛细管区带电泳模式考察并优化了糖类衍生物的分离条件。实验采用58．5cm×50μmi．d．毛血管（有效柱长50cm），55mmol／L硼酸盐缓冲溶液（pH9．46），柱温20℃，分离电压22kV，进样10s，在不加任何添加剂的情况下，高效、快速地实现了9种糖的基线分离，并在最优化条件下进行了唐古特白刺实际样品的分离分析，结果令人满意。

Elongation Factor ELL (Eleven-Nineteen Lysine-rich Leukemia) Acts as a Transcription Factor for Direct Thrombospondin-1 Regulation

The eleven-nineteen lysine-rich leukemia (ELL) gene undergoes translocation and fuses in-frame to the multiple lineage leukemia gene in a substantial proportion of patients suffering from acute forms of leukemia. Studies show that ELL indirectly modulates transcription by serving as a regulator for transcriptional elongation as well as for p53, U19/Eaf2, and steroid receptor activities. Our in vitro and in vivo data demonstrate that ELL could also serve as a transcriptional factor to directly induce transcription of the thrombospondin-1 (TSP-1) gene. Experiments using ELL deletion mutants established that full-length ELL is required for the TSP-1 up-regulation and that the trans-activation domain likely resides in the carboxyl terminus. Moreover, the DNA binding domain may localize to the first 45 amino acids of ELL. Not surprisingly, multiple lineage leukemia-ELL, which lacks these amino acids, did not induce expression from the TSP-1 promoter. In addition, the ELL core-response element appears to localize in the -1426 to -1418 region of the TSP-1 promoter. Finally, studies using zebrafish confirmed that ELL regulates TSP-1 mRNA expression in vivo, and ELL could inhibit zebrafish vasculogenesis, at least in part, through up-regulating TSP-1. Given the importance of TSP-1 as an anti-angiogenic protein, our findings may have important ramifications for better understanding cancer.

温和条件下分子氧的活化和转移——Ⅰ.在三苯基膦和1-辛烯氧化中过氧酞菁钛的氧转移性能的研究

本文研究了过氧酞菁钛(PcTi0_2)在三苯基膦(Ph_3P)以及1-辛烯氧化反应中氧原子转移性能.在温和条件下,PcTiO_2可化学计量地转移一个氧原子至Ph_3P形成Ph_3O.然而,在1-辛烯的氧化反应中,只有在共催化剂,(PhCN)_2PdCl_2存在下,PcTiO_2才能将氧原子转移至被Pd化合物活化的烯烃上形成辛酮.实验结果表明,PcTiO_2在上述两个氧化反应中,只能起转移一个氧原子的作用,不可能形成以分子氧为氧化剂的催化氧化体系.