恒河猴大脑前额叶cDNA 文库的构建

为筛选出与认知、记忆相关的脑部表达基因及基因家族,利用TRIzol试剂从恒河猴大脑前额叶组织抽提总RNA,再用纯化试剂盒从总RNA中成功纯化出mRNA。按照Stratagene公司的cDNASynthesisKit(200401)、ZAP-cDNASynthesisKit(200400)和ZAP-cDNAGigapackⅢGoldCloningKit(200450)三个试剂盒的操作说明,构建了恒河猴大脑前额叶组织的cDNA文库。文库总库容为2·0×106克隆;绝大多数的cDNA插入片段≥0·5kb,平均长度≈1·0kb;cDNA片段与噬菌体载体重组率为97·3%。文库各项指标均达要求,为克隆大脑PFC区表达基因、测定基因编码区序列、揭示具有多种剪切组合模式等提供了可靠资源,也为相关基因表达调控的研究提供了方便。

smoothing document language model with local word graph

ACM SIGIR; ACM SIGWEB

a requirement traceability refinement method based on relevance feedback

Knowledge Systems Institute Graduate School

语句句法成分边界和韵律特征知觉

The research investigates the acoustic-phonetic correlates of various levels of syntactic boundaries and the perception of prosody in Mandarin Chinese, more specifically, the way speakers express the syntatic relations between sentence compounents and teh perceptual representations of prosody. The relation between phonology and syntax in Chinese language is studied by comparing the perceptual representations and syntactic structures of sentences. The results may have theoretical and practical implications for research in fields of speech perception, linguistics and psycholinguistics, and for the development of speech engineering in China.