2 resultados para 1Dx5 1Dy10
em Chinese Academy of Sciences Institutional Repositories Grid Portal
Resumo:
5336021Dx51Dy1041Dx5PCRBC_1F_1BC_2F_1BC_3F_11Dx5BC_3F_1BC_3F_2SDS-PAGEBC_3F_2HMW-GS53346024BC_3F_252510
Resumo:
, HMW- GS , HMW- GS W958T.durum Desf. T.aestivum L1DSDS- PAGE1Dx5, 1Dx51Dx51Dx51Dx21Dx51Dx5N1Dx51Dx51Dx21Dx51Dx51Dx51Dx51Dx51Dx51Dx5SDS-PAGEWheat is one of the major crops utilized worldwide. Nevertheless, due to the lackof the superior HMW- GS, the wheat quality in China is not satisfying. Therefore,identification and characterization of the superior HMW- GS will lay good foundation to the wheat breeding.W958 is a new bread wheat line developed by interspecific cross (T.durum Desf.T.aestivum L). It contains a novel HMW- GS. We have designated such subunit as1Dx5 here for its unique character. To confirm that such subunit is innovative andbeneficial for wheat breeding program on the molecular level, we have investigated itin terms of inheritance, DNA and protein sequence, dough property and agronomictrait associated with it. The result shows that 1Dx5is as superior as 1Dx5 in terms of dough property.In addition, we have cloned the promoter, N- terminal as well as partial centralrepetitive domain of the allele coding for this subunit. Comparison of the amino acidsequence of 1Dx5 with that of 1Dx5 and 1Dx2 showed that the superior quality of1Dx5 subunit may result from the degree of conservation of the repetitive sequencesby which the glutenin polymers interact via inter-chain hydrogen bonds formedbetween the subunit repetitive domains with longer subunits forming more stableinteractions. In addition, we have developed two dominant molecular markers tofacilitate the discrimination of 1Dx5 and 1Dx5 which could no be achieved by SDS-PAGE.
