Bacillus pallidus sp nov., isolated from forest soil

A Gram-positive bacterium, designated strain CW 7(T), was isolated from forest soil in Anhui Province, south-east China. Cells were strictly aerobic, motile with peritrichous flagella and rod-shaped. The strain grew optimally at 30-37 degrees C and pH 7.0-8.0. The major fatty acids of strain CW 7(T) were anteiso-C-15:0, iso-C-15:0 and anteiso-C-17:0. The predominant menaquinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The G + C content of the genomic DNA was 42.3 mol%. Phylogenetic analysis indicated that strain CW 7(T) belonged to a monophyletic cluster within the genus Bacillus and showed 16S rRNA gene sequence similarities of less than 96.5% to recognized species of the genus Bacillus. The results of the polyphasic taxonomic study, including phenotypic, chemotaxonomic and phylogenetic analyses, showed that strain CW 7(T) represents a novel species of the genus Bacillus, for which the name Bacillus pallidus sp. nov. is proposed. The type strain is CW 7(T) (=KCTC 13200(T)=CCTCC AB 207188(T)=LMG 24451(T)).

云南西双版纳片断热带雨林鼠形啮齿类的物种多样性研究

该文以３个不同面积和环境状况的片断热带雨林即西双版纳傣族“龙山林”和１个自然保护区对照样地为工作样区, 对热带雨林片断化后, 鼠形啮齿类物种多样性的变化及其与环境的关系等进行了研究, 结果如下：(1)４个样地铗捕获得的鼠形啮齿类种数分别为３、３、４和５种; (2)城子龙山和保护区对照样地的多样性指数较高(分别为2.058和1.944), 而曼养广龙山和曼俄龙山的物种多样性指数仅为1.165和1.350; (３)各“龙山”的鼠形啮齿类物种多样性与其面积大小的相关性较弱, 而与样地的隔离状况及人为干扰破坏的程度有较显著的相关性; (4)样地内鼠种组成的变化也和样地隔离状态及人为干扰破坏程度的不同密切相关; (5)各样地鼠形啮群落中生物量的分布也很不均匀。

Distribution of microcystins in various organs (heart, liver, intestine, gonad, brain, kidney and lung) of Wistar rat via intravenous injection

The distribution of microcystins (MCs) in various tissues of Wistar rats was studied under laboratory conditions. Rats were injected intravenously (i.v.) with extracted MCs at a dose of 80 mu g MC-LRequivalent/kg body weight. MCs concentrations in various tissues were detected at 1, 2. 4, 6, 12 and 24 h post-injection using liquid chromatography-mass spectrometry (LC-MS). The highest concentration of MCs was found in kidney (0.034-0.295 mu g/g dry weight), followed by lung (0.007-0.067 mu g/g dry weight), stomach (0.010-0.058 mu g/g dry weight) and liver (0.003-0.052 mu g/g dry weight). The maximum MCs content in the whole body of rat, 2.9% of the injected dose, was observed at 2 h post-injection. MCs concentration was higher in kidney than in liver during the experiment, and two peaks of MCs concentration (at 2 and 24 h, respectively) were observed in kidney, indicating that MCs can be excreted directly via kidney of rat. Though heart, intestine, spleen, brain, gonad and stomach contained less than 0.2% of injected MCs during the whole experiment stage, the presence of MCs in these tissues represents potential damage to them. (c) 2008 Elsevier Ltd. All Fights reserved.

Nonylphenol and octylphenol in urban eutrophic lakes of the subtropical China

The paper studied two estrogenic pollutants, 4-nonylphenol (NP) and 4-tert-octylphenol (OP) in water, suspended particle (SP) and sediments in urban eutrophic lakes. The concentrations of NP ranged from 1.94 to 32.85 mu g/l, 0.876 to 31.13 mu g/l and 3.54 to 32.43 mu g/g dry weight (dw) in water, suspended particle (SP) and sediments, respectively, and that of OP from 0.027 to 1.44 mu g/l, 0.008 to 1.777 mu g/l and 0.058 to 1.245 mu g/g dw in water, suspended particle (SP) and sediments, respectively. An increasing trend in the concentration was noticed in all matrices close to the sewage inlets, which was found to be the major factor affecting the spatial distribution of alkylphenols (APs) in the lakes. Due to restoration of submerged macrophytes, which might accumulate APs, the contaminations of APs in the Little Moon Lake (LML) and the Little Lotus Lake (LLL) were lower than those in the Big, Moon Lake (BML) and the Bier Lotus Lake (BLL). A reasonable correlation of NP and OP was obtained among water, suspended particle and sediment. The possible environmental stress of APs concentration on aquatic organisms in Wuhan urban lakes was also discussed.

Tuning of detection wavelength in a resonant-cavity-enhanced quantum-dot-embedded photodiode by changing detection angle

We have fabricated a resonant-cavity-enhanced photodiode (RCE-PD) with InGaAs quantum dots (QDs) as an active medium. This sort of QD-embedded RCE-PD is capable of a peak external quantum efficiency of 32% and responsivity of 0.27A/W at 1.058 mu m with a full width at half maximum (FWHM) of 5 nm. Angle-resolved photocurrent response eventually proves that with the detection angle changing from 0 degrees to 60 degrees, the peak-current wavelength shifts towards the short wavelength side by 37 nm, while the quantum efficiency remains larger than 15%.

Improvement of Ge self-organized quantum dots by use of Sb surfactant

A Sb-mediated growth technique is developed to deposit Ge quantum dots (QDs) of small size, high density, and foe of dislocations. These QDs were grown at low growth temperature by molecular beam epitaxy. The photoluminescence and absorption properties of these Ge QDs suggest an indirect-to-direct conversion, which is in good agreement with a theoretical calculation. (C) 1998 American Institute of Physics. [S0003-6951(98)00420-3].

取代环戊二烯基稀土有机氯化物的合成及其反应

本文通过无水LnCl_3(Ln = Pr.Nd.Gd)与两倍摩尔的叔丁基环戊二烯基碱金属盐在THF中60－80℃反应，分离到一类新的二（叔丁基环戊二烯基）稀土氯化物（T-DuCp）_2 2net.nTHF (Ln = Pr,Nd,n = 2 Ln = Gd,n = 1)，对它们进行了元素分析。红外光谱及水鲜色质谱的表征。对于配合物（t-BuCp）_2PrCl·2THF的单晶，测定了它的晶体结构，晶体层单斜晶系P21/C空间群，晶胞参数为：a = 15.080 b = 8.855 c = 21.196A, β = 110.34°V = 2653.9A~3 δ = 4，结构分析表明此配合物是一中性的单分子配合物。最后的R = Rw = 0.058平均Pr-C.2.81 Pr-Cent 2.53A, Pr-Cl及Rr-O键长分别为2.72与2.62A。本文通过Lnel_3(Ln = Nd.Pr.Ga)与等摩尔的叔丁基环戊二烯基碱金属盐在THF中60－80℃反应，分离到一类中性的单(叔丁基环戊二烯基)稀土二氯化物，并对它们进行了元素分析，红外光谱及水鲜色质谱的表征。本文通过轻稀土元素La,Pr的三氯化物与带基钠以1:2摩尔比在THF中70－80℃反应，分离到了二带基轻稀土氯化物（CaH_7）_2LaCl.2THF及[(CqH_7)_2PrCl.THF]_2。并且对此二配合物进行了元素分析。红外光谱及水鲜色质谱的表征。对于[(CuH_7)_2PrCl.TH]_2配合物，测定了它的晶体结构，这是第一个得到结构表征的茚基稀土氯化物，晶体层于单斜晶系，P_(21)/C空间群，晶胞参数为a = 7.808 b = 17.796 c = 14.070A β = 93.97°v = 1950.31A. E = 2最后的R = 0.045. Rw = 0.039结构分析表明此配合物以中性的二聚体形式存在。平均的Pr-C.2.81 Pr-Cent 2.53. Pr-Cl.2.84H Pr-O钻长2.54A。为了进一步研究不同配体对配合物结构的影响，我们还研究了Gael_3与Nae_5Mes以1:1摩尔比在THF中的反应，分离到了两种配合物[(NaTHF)(C_5MesGd.THF)_2Cl_5]_2.6THF(I)及L_5Me_5GdCl2.3THF(II)并且对配合物（I），测定了它的晶体结构，晶体层于三斜晶系。Pi空间群。晶胞参数a = 12.183 b = 13.638 c = 17.883A, α = 110.38 β = 94.04 γ = 99.44°, V = 2721.20A, E = 1。结构分析表明，此配合物是一种以两个Na原子通过THF中的O原子而桥联的金层有机配合物，在结构上有十分新颖的特点。在此配合物分子中含有四个Gd原子及二个Na原子，Na及Gd间以Cl桥键相联结，Gd-Gd_2 = 4.033 Gdll-Na = 2.818A。最后的R = 0.04M Rw = 0.042。本文还对（t-BuCp）_2P_2Cl.2THF与NaH及LiAlH4的反应进行了初步的研究，分离到（t-Bucp）_2PrH.2THF及(t-Bucp)_2P_2RIH_4.3THF两种新的氢化物，并且对它们进行了元素分析，红外光谱的表征，对于它们水鲜产物的气相分中的H_2，用气相色谱法进行了定性表征。

Syntheses and reactions of heterometallic complexes of nickel and copper with tetrathiotungstates. The molecular structures of [Ph(4)P](2)[S2W(mu-S)(2)Ni(S-2)] and W(mu-S)(4)Cu-2(PPh(3))(4)center dot py

Reaction of [Ph(4)P]2WS4 With NiCl2 in methanol solution in the presence of NaOCH3 leads to the formation of [Ph(4)P](2) [S2W(mu-S)(2)Ni(S-2)] (I) A Similar reaction between (NH4)(2)WS4 and NiCl2 under O-2 atmosphere in the presence of Ph(4)PCl or (n)Bu(4)NCl affords [Ph(4)P](2)([(S-2)W(O)(mu-S)(2)]Ni-2] (IIa) and [(n)Bu(4)N](2)([(S-2)W(O)(mu-S)(2)]Ni-2} (IIb) Under argon the same reaction gives [Ph(4)P](2)[Ni(WS4)(2)] (IIIa) and [(n)Bu(4)N](2)[Ni(WS4)(2)] (IIIb). [Ph(4)P](2)[Ni(WOS3)(2)] (IV) and [Ph(4)P](2)[Ni(WO2S2)(2)] (V) can be prepared from the reaction of [Ph(4)P]2WOS3 and [Ph(4)P]2WO2S2 with NiCl2. Treatment of (NH4)(2)WS4 with CuCl in the presence of PPh(3) in boiling pyridine produces W(mu-S)(4)Cu-2(PPh(3))(3) (VI), which can further react with excess PPh(3) to give W(mu-S)(4)Cu-2(PPh(3))(4) . py (VII). Complex I crystallizes in the space group P2(1)/n with the cell parameters: a = 20.049(4), b = 17.010(4), c = 14.311(7) Angstrom; beta = 110.24(3)degrees and Z = 4; R = 0.058 for 4267 independent reflections. The structural study confirms that complex I contains two terminal sulfide ligands, two bridging sulfide ligands, a side-on disulfide ligand, and a planar central W(mu-S)(2)Ni four membered ring. Complex VII crystallizes in the space group C2/c with the cell parameters: a = 26.436(8), b = 20.542(6), c = 19.095(8) Angstrom; beta = 125.00(3)degrees and Z = 4; R = 0.080 for 3802 independent reflections. The structural study reveals a perfect linear arrangement of the three metal atoms Cu-W-Cu.

Determination of five pharmacologically active compounds extracted from Rhodiola for natural product drug discovery with HPLC-APCI-MS

A rapid and sensitive liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (HPLC-APCI-MS) assay for the determination of five pharmacologically active compounds (PAC) extracted from the traditional Chinese medicine, Rhodiola , namely salidroside, tyrosol, rhodionin, gallic acid, and ethyl gallate has been developed. In this method, PAC could be baseline separated and detected with DAD at 275 nm. The validation of the method, including sensitivity, linearity, repeatability, and recovery, was examined. The linear calibration curves were acquired with correlation coefficient >0.999 and the limits of detection LOD (at a signal-to-noise ratio=3:1) were between 0.058 and 1.500 mu mol/L. It was found, that the amounts of PAC varied with different species of Rhodiola . The established method is rapid and reproducible for the separation of five natural pharmacologically active compounds from extracts of Rhodiola with satisfactory results.

河西走廊棘豆毒草中的硒及其与牲畜中毒关系研究

河西走廊地区草原生态环境,棘豆等毒草泛滥成灾,导致大量牲畜中毒死亡。棘豆毒草导致的牲畜中毒症状与历史上马可波罗记载的古代肃州牲畜毒草中毒症状及其相似。为了确定马可波罗所记载的肃州牲畜毒草中毒是否与硒有关,本项研究对河西走廊地区主要草原畜牧区肃南县草原发育的棘豆属植物及其土壤中的硒进行了测定,测定结果表明:土壤硒含量平均值为0.205±0.173mg/kg,与世界平均土壤硒含量相近。棘豆属植物小花棘豆根、茎叶的硒平均含量分别为0.112±0.038mg/kg(0.052～0.174mg/kg),0.102±0.027mg/kg(0.066～0.158 mg/kg);黄花棘豆全株硒平均含量为0.066±0.009mg/kg(0.058～0.078mg/kg),种子硒:0.107mg/kg。可见该区棘豆属毒草硒含量远低于能导致一般动物中毒的3mg/kg植物硒含量。根据肃南县棘豆毒草发育的地理位置、牲畜中毒症状调查及其硒含量测定的结果,本文认为马可波罗所记载的中国西部肃州牲畜毒草中毒是棘豆属毒草中毒,其主要毒素成分不是硒。