链霉菌S227抗耐药菌活性成分的研究

本论文以从四川峨嵋山森林土壤中分离筛选获得的一株产抗耐药性活性化合物的链霉菌S227为材料，对发酵液中活性物质的分离纯化及抗耐药性活性进行了研究。 建立了抗耐药性活性的定性、定量检测方法。建立的管蝶法活性定量检测的标准回归方程为：D=4.8229Ln(C)+3.6326 R=0.9972 ；纸片法活性定量检测的标准回归方程为：D=5.5Ln(C)-12.794 R=0.999。 根据建立的样品活性的检测方法，测定了发酵液的初始活性。实验证明活性物质的温度、pH稳定性好。 通过活性的定性、定量追踪方法，分别利用等体积的石油醚、乙酸乙酯、正丁醇在不同的pH梯度下萃取，确定了pH3条件下正丁醇能最大程度的萃取活性物质，说明活性物质极性很大。对正丁醇萃取相经过两次硅胶柱层析及薄层层析分离得到具有抗耐药菌活性的纯化样品S227-4。 经过核磁共振氢谱、碳谱数据分析初步确定S227-4为四聚糖，通过糖的水解实验初步确定S227-4由葡萄糖和半乳糖组成。 纸片法活性检测表明S227-4具有抗耐药菌活性。采用MIC测定法对该样品抗耐药活性进行研究。在证明该样品本身不具有抗菌活性的基础上，以临床分离的耐药性金黄色葡萄球菌为指示菌，考察了该样品与抗生素联合使用时对耐药菌抗生素MIC（最小抑菌浓度）值的影响，结果表明在不影响菌体生长的浓度条件下，该样品能明显降低多株耐药菌对多种抗生素的MIC值，不同程度地恢复所测试耐药菌对相应抗生素的敏感性。如S227-4与青霉素钠联用可以使S. aureus 12352的MIC降低8倍，而与红霉素联用可以使S. aureus 12334的MIC降低128倍。 The purification process and the activity of the anti bacterial drug resistance compounds produced by Streptomyces S227 isolated from the forest soil sample of the Mountain E’MEI in Sichuan Province were studied in this thesis. Quantitative and qualitative activity assay methods of the active compounds were established. The regression equation of the tube method was D=4.8229Ln(C)+3.6326, R=0.9972. The regression equation of the paper method was D=5.5Ln(C)-12.794, R=0.999. According to the established activity assay method, the incipient activity of the broth was evaluated. And it was proved that the stability of the active compounds was good. By quantitative and qualitative activity tracing method, petroleum ether, ethyl acetate and butanol were used to extract the active compounds at different pH. The result showed that butanol was the most effective agent for active component recovery at pH3. From the butanol extraction a purified sample, S227-4, was isolated by silica gel column chromatography and thin-layer chromatography . S227-4 was proved to be a tetra- saccharide by 1H-NMR and 13C-NMR. And its monosaccharides include glucose and galactose by hydrolysate analysis. The anti-drug resistant activity of S227-4 was tested in vitro by MICs assay using different drug resistant Staphylococcus aureus strains isolated clinically. The sample itself showed no anti-microbial activity in growth inhibitory experiment, but when it was used together with different antibiotics, it could remarkably decrease the MICs of different clinically isolated drug-resistant bacterial strains to these antibiotics. For example, when S227-4 was used with penicillin, the MIC of S. aureus 12352 decreased 8 times compared with that when penicillin was used alone. Meanwhile when it was used with erythromycin the MIC of S. aureus 12334 deceased 128 times compared with erythromycin alone.

短寿命缺中子同位素~(130,129,128)Ce的(EC+β~+)衰变

首次报道130Ce、129Ce和128Ce的(EC+β+)衰变纲图,其中包括核素的产生、核素的分离鉴别以及纲图的建立。着重补充了一些重要的技术细节,更新了129Ce的衰变纲图,并对这些纲图中涉及的物理问题进行了讨论。

缺中子~(130)Pm和~(128)Pr核的EC/β~+衰变研究

171MeV 36Ar束轰击96Ru靶由熔合蒸发反应生成了130 Pm和12 8Pr核 .由氦喷嘴快速带传输系统将反应产物送到低本底区 .基于X γ t,γ γ t符合测量 ,首次建立了130 Pm的 (EC + β+)衰变纲图 .重新研究了12 8Pr核的衰变 ,订正和补充了γ射线 ,建立了新的 (EC + β+)衰变纲图

A New High-Spin Level Scheme of I-128

High-spin states in the odd-odd nucleus I-128 are investigated via the Sn-124(Li-7,3n)I-128 reaction at 28 and 32 MeV beam energies. A new level scheme of I-128 is established up to high-spin states at I-pi = 16, including 48 levels and 72 gamma transitions. The present level scheme is largely different from the one in a recent publication due to identification of several doublet and triplet gamma transitions and their proper placements in the level scheme. The high-spin level structure exhibits no obvious collective properties and is possibly associated with two and multi-quasiparticle configurations.

潘洛铁矿滑坡特征分析及防治

分析了潘洛铁矿露天矿滑坡灾害发生发展的特征,得出该滑坡是一个动态的、多层次的复合型滑坡;找出了影响该边坡稳定的主要因素是大气降雨和采矿切割坡脚;根据这些影响因素和滑坡发展变化规律,提出了科学合理的滑坡治理方案,即动态的综合治理方案.经现场实施后,通过滑坡监测数据表明,该滑坡得到了彻底根治.

Molecular dynamics simulation of hydrogen enhancing dislocation emission

The interactive pair potential between Al and H is obtained based on the ab initio calculation and the Chen-Mobius 3D lattice inversion formula. By utilizing the pair potentials calculated, the effects of hydrogen on the dislocation emission from crack tip have been studied. The simulated result shows that hydrogen can reduce the cohesive strength for Al single crystal, and then the critical stress intensity factor for partial dislocation emission decreases from 0.11 MPa root m (C-H = 0) to 0.075 MPa root m (C-H=0.72%) and 0.06 MPa root m (C-H = 1.44%). This indicates thar hydrogen can enhance the dislocation emission. The simulation also shows that atoms of hydrogen can gather and turn into small bubbles, resulting in enhancement of the equilibrium vacancy concentration.

Rheological flow from a die and painting on a moving solid wall

Die swell is an important, phenomenon. in polymer processing, and is explained usually by rheological properties of the fluid. Because of the nonuniform of temperature distribution on the free surface of the liquid jet, the thermo capillary convection driven by surface tension gradient exists. The rheological fluid flowing out of a die and painting on a moving solid wall is studied by the numerical finite element method of a two-dimensional and unsteady model in the present paper, and both the rheological effect of a non-Newtonian fluid and the thermocapillary effect are considered. The results show that both,effects; will enlarge the cross-section of the fluid jet, and the rheological effect of non-Newtonian fluid dominates the process in general.

电子束引起的热击波和层裂破坏效应(英文)

本文对强流低能相对论电子束引起LT－12铝产生的热击波和层裂进行了分析．电子束在靶材中的能量沉积是非常复杂的，通常都用Monte－Carlo数值模拟的方法来计算能量沉积，本文发展了一种新的半经验解析方法．在研究由电子束引起材料的动态响应时，对于靶材表面的熔化过程要进行适当考虑，本文使用了GRAY三相状态方程，详细考虑了这种复杂的溶化过程．同时在研究层裂破坏效应及应力波的衰减规律时，放弃了通常的von—Mises流体弹塑性模型，而使用了应变率相关的本构模型．计算结果和实验结果比较一致．

一种新的破坏模式：反冲塞

观察到了长脉冲激光束辐照到铜薄片上产生的新的破坏模式．这种破坏可以分为三个阶段：鼓包，剪垣变形局部化及由冲塞所引起的贯穿，这种破坏模式的典型特征是鼓包和冲塞都是沿着入射激光相反的方向进行的，它同人们所塾知的激光引起材料破坏的分裂、烤化和汽化等不同，激发束的特殊的空间分布造成了这种新的破坏模式．

脉冲风洞中用油滴表面流动显示技术研究激波-边界层干扰流动

本文介绍一种适合于脉冲风洞中显示复杂流动的表面油滴技术及其实验结果。在实验时间为20至500ms 的风洞中用表面油滴技术能清晰地显示尖前缘翼诱导激波-边界层干扰流表面流谱的详细特征,其特征位置与用铂薄膜电阻温度计和液晶热图测量结果吻合。油流图像表明尖翼高超声速干扰流具有相似于超声速干扰流的锥形特性,大翼偏转角时,存在二次分离再附现象。实验结果证实了尖翼上游干扰角的高超声速相似特性。

可压缩流边界层参数的工程计算方法

本文把边界层理论中的参考焓方法推广到具有压力梯度的二维和三维轴对称流动,得到了物面动量厚度和摩阻的计算公式.同时利用边界层理论的某些成果推导了边界层各种厚度之间的近似关系.所得公式计算方便,与目前流行的但只适用于特殊情况的公式和有关的实验数据的比较表明,本文的结果是满意的.