Conformational transition of DNA in electroreduction studied by in situ UV and CD thin layer spectroelectrochemistry

Electrochemically induced three conformational transitions of calf thymus DNA from B-10.4 to Z(10.2)-DNA and from B-10.2 to B-10.4 and to C-DNA in 10 mM phosphate buffer solution (pH 7.21) at glassy carbon electrode are found and studied by in situ circular dichroism (CD) thin layer spectroelectrochemistry with singular value decomposition least square (SVDLS) analysis. It indicates that the so-called B-10.2 form and the C-form of DNA may be composed of B-10.4 and left-A DNA and of B-10.4 and right-A DNA, respectively. The irreversible electrochemical reduction of adenine and cytosine groups in the DNA molecule is studied by UV-Vis spectroelectrochemistry. Some electrochemical parameters alphan = 0.17, E-0' = -0.70 V (vs. Ag/AgCl), and the standard heterogeneous electron transfer rate constant, k(0) = 1.8 x 10(-5) cm s(-1) are obtained by double logarithmic analysis and non-linear regression. (C) 2000 Elsevier Science B.V. All rights reserved.

The synthesis of phenyl-capped trianiline and teraaniline by a modified-pseudo-high dilution technique and their UV-Vis spectral studies

Phenyl-capped trianiline and tetraaniline in the leucoemeraldine oxidation state were synthesized through a modified-pseudo-high-dilution technique. The chemical oxidation process of these compounds were studied by UV-Vis spectra. It was found that phenyl-capped trianiline in the leucoemeraldine oxidation state was oxidized to its EB form and then decomposed, Phenyl-capped tetraaniline was oxidized to its EB form and then to the pernigraniline oxidation state.

Zn_2SiO_4∶Mn的VUV和UV光谱特性

采用高温固相反应法以硅酸为原料合成了等离子显示用荧光体Zn2 SiO4 ∶Mn ,研究了Zn2 SiO4 ∶Mn的VUV和UV光谱特性 ,表明波长小于 2 0 0nm的部分的基质吸收带主要是氧的 2 p轨道到锌的 3d轨道跃迁产生的 ,波长大于 2 0 0nm的部分的基质吸收带是氧的 2 p轨道到硅的 3p轨道跃迁吸收。在VUV和UV激发下 ,Mn2 +的浓度与发射强度的相关性研究表明 ,在不同区域激发时荧光体的发射强度随着Mn2 +的浓度的变化存在明显不同

Luminescent properties of Gd3PO7 : Eu in UV/VUV region

The luminescent properties of Gd3PO7:Eu were investigated in W and VUV regions. This phosphor has such strong emissions around 618.5 nn under UV excitation, especially around 209nn, that it has a better colour saturation than that of Y2O3:Eu. It could be a new potential red phosphor for lamp and other applications with W-excitation source or even for displaying devices. In the VUV excitation spectrum of Eu3+ emissions, energy absorptions by Gd3+, Gd-O and PO43- have been observed, on which it can be inferred that there are three kinds of energy transferring processes to Eu-3(+) ions. The energy absorptions of Gd3+, Gd-O and PO43- are induced by 4f --> 4f transitions, a bandgap transition of Gd3PO7 and intramolecular (2t(2) --> 2a, 3t(2)) transition, respectively. The energy transfer efficiency of the PO43- intramolecular transition is lower compared with that of the bandgap transition (Gd-O).

NMR and UV-Vis study on phenyl-capped oligoaniline salts

Phenyl-capped oligoanilines in the oxidized state and their salts (by camphor sulfonic acid, CSA) were comparatively studied by UV-Vis and NMR spectroscopy. The UV-Vis spectra revealed a similar electron transfer behaviour and similar structure in the tetraaniline to those in polyaniline. Upon formation of the salt, H-1 NMR CH peaks of the oligomers showed large shifts to lower fields. The longer the molecule of a oligomer is, the more the shift, indicating that the charge brought into the N atoms by the proton was redistributed over the whole molecule. The CH and quaternary carbon peak-shifts support the electron cloud motion route H -->C -->C -->N -->H. This is in agreement with the four ring BQ derivatives model.

Production of active oxygen species from Taxus cuspidata induced by far-UV radiation

In order to understand the role of active oxygen species in mediating plant injuries induced by far-UV radiation, seedlings of Taxus cuspidata Sieb. et Zucc. were irradiated by far-UV rays in laboratory for 4 weeks. The production of organic free-radicals in detached needles, and the production of O-2(radical anion) and O-1(2) in isolated chloroplasts were detected weekly by electron spin resonance (ESR) to evaluate their relative importance. The results show that the cumulative effect of far-UV irradiation, is best indicated by the production of organic free radicals in the needles, O-2(radical anion) production in chloroplasts is the next. The enhancement of O-1(2) production in chloroplasts by the cumulative far-UV irradiation seems to be not so important as O-2(radical anion) in mediating injuries induced by, far-UV radiation because of its high background value.

Synthesis of monodisperse pheyl-capped oligoaniline pentamer and hexamer in the leucoemeraldine oxidation state and EB state and its UV studies

Aniline pentamer and hexamer in the leucoemeraldine oxidation state were synthesized through a novel method. The method was accomplished by the reaction of parent aniline tetramer in the pernigraniline oxidation state with diphenylamine and N-phenyl-1,4-phenylenediamine in the leucoemeraldine oxidation state respectively. The oligomers in the leucoemeraldine oxidation state were characterized by IR, NMR, elemental analysis and MALDI-MS. Aniline pentamer and hexamer in the emeraldine oxidation state were synthesized by the oxidation of Ag2O in DMF. It was found that some fragmentation occurred when the pentamer and hexamer were oxidized by (NH4)(2)S2O8 and FeCl3. 6H(2)O. The pentamer and hexamer in the emeraldine oxidation state was studied by UV/Vis spectra. The relative intensity of exciton peak for pentaaniline showed a little increase compared with that of hexaaniline.

Combination of amperometric detector & UV detector for capillary electrophoresis

A new electrochemical cell assembly with the combination of UV and amperometric detector (AD) based on their complementarity was described. A Nafion tubing junction was used to decouple the high voltage from the separation capillary in the rear of on-column UV detector. In this mode, the electroactive and inert compounds could be detected by UV and AD at the same time. Aromatic amines were determined with the UV and the end-column AD detection to evaluate the performances of the cell assembly. Such an improved electrochemical detector could match the capillary with different diameters. By simple adjustment of the screws, the positioning of the working electrode and the detection capillary was easily gained without microscope. It is also very easy to assemble and disassemble the working electrode when needed. (C) 1999 Elsevier Science B.V. All rights reserved.

In situ UV-vis and CD thin-layer spectroelectrochemistry study of the electrochemical behavior of L-noradrenaline in alkaline solution

Electrochemical redox behavior of noradrenaline in alkaline solution on a glassy carbon electrode has been investigated by in situ UV-vis and CD spectroelectrochemistry by using a long optical path thin-layer cell. The experimental data were processed by using a double logarithmic method of analysis together with nonlinear regression which confirmed that the first step in both the oxidation of noradrenaline and reduction of noradrenochrome is a two-electron irreversible process governed by an EE mechanism. The kinetic parameters of the electrode reactions, i.e., charge transfer coefficient and the number of electrons transferred, alpha(1)n(1) = 0.11 and alpha(2)n(2) = 0.23, formal potentials modified with kinetics, E-1(0') = 0.65 (+/- 0.01) V and E-2(0') = 0.72V and standard rate cnstants, k(1)(0) = 7.0(+/-0.5)x10(-5) cm s(-1), for the first and second steps in the oxidation process of noradrenaline, and similarly, alpha(1)n(1) = 0.33, alpha(2)n(2) = 0.58, E-1(0') = 0.37(+/-0.01) V, E-0' = -0.25 (+/-0.01) V and k(1)(0) approximate to k(2)(0) = 1.06 (+/-0.05)x10(-4) cm s(-1) for the first and second steps in the reduction process of noradrenochrome were also determined.

卟啉和卟啉铜分子的成膜特性和UV－Vis光谱表征

合成了５，１０，１５，２０－四（对－异戊酯苯基）卟啉及其铜配合物，研究了它们在气液界面的成膜性质，制备了上述化合物的ＬＢ膜，利用ＵＶ—Ｖｉｓ光谱对化合物在ＬＢ膜结构中的取向进行了表征．研究结果表明．两种化合物在界面上的成膜性质和分子环面在ＬＢ膜结构中的取向均受到铜离子配位的影响.

IN-SITU UV-VISIBLE SPECTROELECTROCHEMICAL AND CIRCULAR DICHROIC ELECTROCHEMICAL STUDY OF BILIRUBIN AND BILIRUBIN PLUS HUMAN SERUM-ALBUMIN COMPLEX

The electro-oxidation of bilirubin (BR) in aqueous solution was investigated by cyclic voltammetry and in-situ thin-layer spectroelectrochemical techniques, It was found that both oxidation processes of BR are two electron transfer reactions. A mechanism

DETERMINATION OF THE MOLECULAR-ORIENTATION OF A PHTHALOCYANINE DERIVATIVE IN A LANGMUIR-BLODGETT-FILM BY POLARIZED UV VIS SPECTRA

Langmuir-Blodgett (LB) films of (p-carboxyphenoxy)-tri(2,4-di-tert-pentyl phenoxy)phthalocyanine copper(II) (asyCuPc) are prepared; the associated forms of the compound in chloroform solution and the particular orientation of asyCuPc molecular macrocycles in LB films is determined by polarized UV-VIS.

Lack of genetic differentiation in the shrimp Penaeus chinensis in the northwestern pacific

Genetic differentiation of the shrimp Penaeus chinensis in the Yellow Sea and Bohai Sea was investigated using the mitochondrial control region (CR). RFLP of a partial CR segment (613 bp) shows that 106 out of 122 (86.9%) individuals from six sampling localities along the coast of northern China and the west coast of the Korean Peninsula share the same haplotype, and the haplotype frequencies among localities are not significantly different. The findings are further confirmed by sequencing the complete CR. Divergence of the complete CR (992 bp) is less than 1.6% in 14 individuals from the six localities. F-statistics based on RFLP data and the TCS network of sequencing data suggest little genetic differentiation of P. chinensis in the Yellow Sea and Bohai Sea. Mismatch analysis suggests a rapid expansion of P. chinensis population to the Yellow Sea and the Bohai Sea, which probably occurred with the rapid rise in sea level after the last glacial maximum. Despite the lack of genetic heterogeneity, we propose that P. chinensis populations in this region should be treated as separate management units, as fishery management programs have to be applied on a local basis by different governments.

高寒草甸植物抗氧化系统对长期增强UV-B辐射的响应

通过研究自然条件下模拟平流层臭氧破坏5%时近地表面增加的太阳UV-B辐射对高寒草甸4种典型植物（矮嵩草Kobresia humilis、垂穗披碱草Elymus nutans、麻花艽Gentiana straminea和鹅绒委陵菜Po-tentilla anserina）的抗氧化系统的影响表明,尽管各植物的抗氧化系统组分变化不同,但4种植物的膜脂过氧化程度没有加剧,长期增强UV-B辐射没有对膜系统造成损伤。在自然长期增强UV-B条件下,4种植物的膜脂过氧化产物——丙二醛（MDA）含量与对照相比无显著差异。垂穗披碱草、鹅绒委陵菜的谷胱甘肽（GSH）含量增加,麻花艽的超氧化物歧化酶（SOD）活性与鹅绒委陵菜的过氧化氢酶（CAT）活性上升,同时麻花艽的类胡萝卜素（Car）含量亦显著增加。可见这些植物已能很好地适应UV-B强辐射,其抗氧化能力除了与抗氧化系统各组分的协同作用有关外,也可能与种的适应性有关。