Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai-Tibet Plateau

Swertia przewalskii Pissjauk. (Gentianaceae) is a critically endangered and endemic plant of the Qinghai-Tibet Plateau in China. RAPD and ISSR analyses were carried out on a total of 63 individuals to assess the extent of genetic variation in the remaining three populations. Percentage of polymorphic bands was 94% (156 bands) for RAPD and 96% (222 bands) for ISSR. A pairwise distance measure calculated from the RAPD and ISSR data was used as input for analysis of molecular variance (AMOVA). AMOVA indicated that a high proportion of the total genetic variation (52% for RAPD and 56% for ISSR) was found among populations; pairwise Phi(ST) comparisons showed that the three populations examined were significantly different (p < 0.001). Significant genetic differentiation was found based on different measures (AMOVA and Hickory theta(B)) in S. przewalskii (0.52 on RAPD and 0.56 on ISSR; 0.46 on RAPD and 0.45 on ISSR). The differentiation of the populations corresponded to low average gene flow (0.28 based on RAPD and 0.31 based on ISSR), whereas genetic distance-based clustering and coalescent-based assignment analyses revealed significant genetic isolation among populations. Our results indicate that genetic diversity is independent of population size. We conclude that although sexual reproduction and gene flow between populations of S. przewalskii are very limited, they have preserved high levels of genetic diversity. The main factors responsible for the high level of difference among populations are the isolation and recent fragmentation under human disturbance.

Seasonal patterns of gross primary production and ecosystem respiration in an alpine meadow ecosystem on the Qinghai-Tibetan Plateau

We measured the net ecosystem CO2 exchange (NEE) in an alpine meadow ecosystem (latitude 37degrees29'-45'N, longitude 101degrees12'-23'E, 3250 m above sea level) on the Qinghai-Tibetan Plateau throughout 2002 by the eddy covariance method to examine the carbon dynamics and budget on this unique plateau. Diurnal changes in gross primary production (GPP) and ecosystem respiration (R-e) showed that an afternoon increase of NEE was highly associated with an increase of R-e. Seasonal changes in GPP corresponded well to changes in the leaf area index and daily photosynthetic photon flux density. The ratio of GPP/R-e was high and reached about 2.0 during the peak growing season, which indicates that mainly autotrophic respiration controlled the carbon dynamics of the ecosystem. Seasonal changes in mean GPP and R-e showed compensatory behavior as reported for temperate and Mediterranean ecosystems, but those of GPP(max) and R-emax were poorly synchronized. The alpine ecosystem exhibited lower GPP (575 g C m(-2) y(-1)) than, but net ecosystem production (78.5 g C m(-2) y(-1)) similar to, that of subalpine forest ecosystems. The results suggest that the alpine meadow behaved as a CO2 sink during the 1-year measurement period but apparently sequestered a rather small amount of C in comparison with similar alpine ecosystems.

孤南-三合村洼陷含油气系统研究

The development petroleum geology has made people from studying and studying and predicting in statically and respectively the pool-forming conditions of an area such as oil source bed, reservoir, overlying formation, migration, trap and preservation, etc. to regarding these conditions as well as roles of generation, reservation and accumulation as an integrated dynamic evolution development system to do study .Meanwhile apply various simulating means to try to predict from quantitative angle. Undoubtedly, the solution of these questions will accumulate exploration process, cut down exploration cost and obtain remarkable economic and social benefits. This paper which take sedimentology ,structural geology and petroleum geology as guides and take petroleum system theory as nucleus and carry out study thinking of beginning with static factor and integration of point and face as well as regarding dynamic state factor as factor and apply study methods of integration of geology, Lab research and numerical modeling proceed integrated dissect and systematic analysis to GuNan-SanHeCun depression. Also apply methods of integration of sequence stratigraphy, biostratigraphy, petrostratigraphy and seismic data to found the time-contour stratigraphic framework and reveal time-space distribution of depositional system and meantime clarify oil-source bed, reservoir and overlying distribution regular patterns. Also use basin analysis means to study precisely the depositional history, packed sequences and evolution. Meanwhile analyze systematically and totally the fracture sequence and fault quality and fault feature, study the structural form, activity JiCi and time-space juxtaposion as well as roles of fault in migration and accumulation of oil and gas of different rank and different quality fault. Simultaneously, utilize seismic, log, analysis testing data and reservoir geology theory to do systematic study and prediction to GuNan-SanHeCun reservoir, study the reservoir types macroscopic distribution and major controlling factors, reservoir rock, filler and porosity structural features as well as distribution of reservoir physical property in 3D space and do comprehensive study and prediction to major controlling and influential factors of reservoir. Furthermore, develop deepingly organic geochemistry comprehensive study, emphasis on two overlaps of oil source rock (ESI, ES3) organic geochemistry features, including types, maturity and spatial variations of organic matter to predict their source potential .Also apply biological marks to proceed oil-to-source correlation ,thereby establish bases for distribution of petroleum system. This study recover the oil generation history of oil source rocks, evaluate source and hydrocarbon discharge potential ,infer pool-forming stages and point out the accumulation direction as well as discover the forming relations of mature oil-source rock and oil reservoir and develop research to study dynamic features of petroleum system. Meanwhile use systematic view, integrate every feature and role of pool forming and the evolution history and pool-forming history, thereby lead people from static conditions such as oil source bed, reservoir, overlying formation, migration, trap and preservation to dynamically analyzing pool-forming process. Also divide GuNan-SanHeCun depression into two second petroleum system, firstly propose to divide second petroleum system according to fluid tress, structural axis and larger faults of cutting depression, and divide lower part of petroleum system into five secondary systems. Meanwhile establish layer analysis and quantitative prediction model of petroleum model, and do quantitative prediction to secondary petroleum system.

大别（超）包产到户铁岩和阜新火山岩铂族元素斌存规律研究

The platinum-group elements (PGE), including Os, Ir, Ru, Rh, Pt and Pd, axe strongly siderophile and chalcophile. On the basis of melting temperature, the PGE may be divided into two groups: the Ir group (IPGE, >2000°C) consisting of Os, Ir and Ru, and the Pd group (PPGE, <20GO°C) consisting of Rh, Pt and Pd. Because of their unique geochemical properties, PGE provide critical information on global-scale differentiation processes, such as core-mantle segregation, late accretionary history, and core-mantle exchange. In addition, they may be used to identify magma source regions and unravel complex petrogenetic processes including partial melting, melt percolation and metasomatism in the mantle, magma mixing and crustal contamination in magma chambers and melt crystallization.Compared with other rocks, (ultra)mafic rocks have lower REE content but higher PGE content, so PGE have advantages in studying the petrogeneses and evolution of them. In this study, we selected (ultra)mafic rocks collected in Dabie Orogen and volcanic rocks from Fuxin Region. Based on the distribution and behaviour of platinum-group elements, combined with other elements, we speculate the magma evolution and source mantle of these (ultra)mafic rocks and volcanic rocks.Many (ultra)mafic rocks are widely distributed in Dabie Region. According to their deformation and metamorphism, we classed them into three types. One is intrusive (ultra)mafic rocks, which are generally undeformed and show no or little sign of metamorphism, such as (ultra)mafic intrusions in Shacun, zhujiapu, Banzhufan, qingshan, Xiaohekou, Jiaoziyan, Renjiawan and Daoshichong. The other one is ultrahigh pressure metamorphic (ultra)mafic rocks, some of them appeared as eelogites, such as complex in Bixiling and adjacent Maowu. Another one is intense deformed and metamorphic, termed as tectonic slice, alpine-type (ultra)mafic rocks. The most representative is Raobazhai and Dahuapin. However, there are many controversies about the formation of those (ultra)mafic rocks. Here, we select typical rocks of the three types. The PGE were determined by inductively coupled plasma mass spectrometry (ICP-MS) ater NiS fire-assay and tellurium co-precipitation.The PGE tracing shows that three components are needed in the source of the cretaceous (uitra)mafic intrusions. They could be old enriched sub-continental lithospheric mantle, lower crust and depleted asthenospheric mantle. The pattern of PGE also shows the primitive magma of these intrusions underwent S saturation. According to palladium, we can conclude that the mantle enrich in PGE. Distribution of PGE in Bixiiing and Maowu (ultra)mafic rocks display they are products of magmas fractional crystallization. The (ultra)mafic rocks in Bixiiing and Maowu are controlled by various magmatic processes and the source mantle is depleted in PGE. Of interest is that the mantle produced UHP (ultra)mafic rocks are PGE-depleted, whereas the mantle of cretaceous (ultra)mafic intrusions are enrich in PGE. This couldindicate that the mantle change from PGE-enriched to PGE-depleted during120-OOMa, which in accord with the time of tectonic system change in the East China. At the same time, (ultra)mafic intrusions in cretaceous took information of deep mantle, which means the processes in deep mantle arose structural movement in the crust The character of PGE in alpine-type (ultra)mafic rocks declared that the rocks had experienced two types of metasomatic processes - hydrous melt derived from slab and silicate melt. In addition, we analyze the platinum-group elements in volcanic rocks on the northern margin of the North China Craton, Fuxin. The volcanic rocks characterized by negative anomalies of platinum. This indicates that platinum alloys, which may host some Pt resided in the mantle. The PGE patterns also show that Jianguo alkali basalts derived from asthenospheric mantle source, but wulahada high-Mg andesites derived from lithospheric mantle.

亚洲地形和海陆分布变化对东亚环境格局形成演化的作用：数值模拟与地质记录对比

Earlier studies on the distribution of geological environmental indicators in China revealed drastic changes from a zonal climate pattern (planetary-wave-dominant pattern) in the Paleogene to a monsoon-dominant one in the Neogene, which suggested an inception of the initial East-Asian summer monsoon. However, there are different views about the time and causes of the changes.Here, we attempt to compile a series of paleoenvironmental maps based on newly collected climate indicators from the literatures and chronologically constrained evidence of geological maps in order to re-examine the temporal and spatial evolution of climate belts in China during the Cenozoic with special emphasis on the changes of the arid belt. These indicators include mammalian fauna, coal, carbonate concretions, jarosite, salt, gypsum deposits and pollen assemblages etc, with chronological controls that we believe reliable. Pollen assemblages and mammalian fauna have been classified into three categories (arid, semi-arid/sub-humid, humid) to reflect the intensity of aridity/humidity. Salt, jarosite and gypsum deposits are classified as the arid indicators. Carbonate concretions and coal are classified into the semi-arid/sub-humid and humid one respectively. Paleoenvironmental maps at 8 time slices have been reconstructed. They are the Paleocene, Eocene, Oligocene, Miocene, Early Miocene, Middle Miocene, Late Miocene and Pliocene.And furthermore, we attempt to use IAP^AGCM to simulate the evolution of climate belts in emphasizing on the changes of the rain band, and compare the results with the paleoenvironmental maps in order to examine the causes of the drastic paleoenvironmental changes near the Oligocene/Miocene boundary. 36 sensitive numerical experiments are carried out using the IAP__AGCM to analyze the impacts of the uplift of the Himalayan-Tibetan complex, shrinkage of the Paratethys Sea, expansion of the South China Sea and the development of the polar ice sheets on rain band in China.The main conclusions are as follows:The obtained results essentially confirm the earlier conclusions about a zonal climate pattern in the Paleogene and a different pattern in the Neogene, and illustrate that a monsoon-dominant environmental pattern with inland aridity formed by the Early Miocene, which is temporally consist with the onset of eolian deposits in China.Cenozoic cooling and the formation of polar ice sheets are unlikely the main causes to the changes of environmental patterns mentioned above in China. But northern hemispheric cooling and the ice-sheets can intensify the Siberian High Pressure, and strengthen the winter monsoon circulations and enhance the aridity in the west part of China. These results support the earlier studies.Shrinkage of the Paratethys Sea and uplift of the Himalayan-Tibetan complex played important roles in strengthening the East Asian monsoon and induceing the above changes of environmental pattern, which is consistent with the earlier studies. Furthermore, "the monsoon-dominant pattern" appears when the Himalayan-Tibetan complex reaches to about 1000-2000 meters high and the Paratethys Sea retreats to the Turan Plate.4) Expansion of the South China Sea is another significant factor that drives the evolution of environmental patterns. We believe that the above three factors co-act and drive the change of the environmental patterns from a planetary-wave-dominant one to a monsoon-dominant one. However, the impacts of each factor vary by regions. The uplift mainly increases the humidity in Southwestern China and the aridity in northwestern country. The shrinkage mainly increases the humidity in Northern China and also enhances the aridity in the northwestern country. The expansion greatly increases the humidity in the south part of China.

工作社会规范观念的心理学研究与跨文化比较

Data on meaning of working (MOW) were collected from 905 respondents in Beijing area through a stratified sampling procedure of the questionnaire used in the restudy of meaning of working (which is still on going, adapted for using in China). Some parts of the factor structure obtained in this study are similar to what found by the MOW International Research Team (1987). Detailed analysis and cross-cultural comparisons were concentrated on the most similar parts societal norms about working (SNW). T-scores of the obligation norm (ON) and entitlement norm (EN) of Chinese were calculated based on the reaction frequencies of relevant items provided by the MOW International Research Team and shown as follows. The results reveal the obligation orientation characteristic of Chinese respondents. And more, 4 SNW patterns of Chinese respondents were obtained through multivariate cluster analysis and multivariate discrimination analysis and shown as follows. The influences of antecedent variables on SNW and the consequences of SNW were analyzed. Two questions were raised from these analyses: 1) The present measurements of SNW are not able to reflect the nature of the relation and dependency between ON and EN and are not free from social desirability; 2) the relationship between SNW and actual working behaviors need to be tested while the relationship between SNW and several work intentions was confirmed. A forced-choice scale of SNW was designed to overcome the shortcomes of the original scale. This scale includes 30 items. Each item is composed of a ON statement and a EN statement with similar values of social desirability. A repertory grids test was used to exam the constructure validity and to analyze the differences of cognitive structures of the 4 SNW patterns. The results indicated that the new scale had a good discrimination power. An experiment was designed to test the relationship of SNW and the actual work behaviors of the respondents. The respondents' action in completing a task was chosen as working behavior index. 4 hypotheses were tested: 1) higher obligation orientation subjects are more likely to complete tasks no matter overtime; 2) scheduled tasks are more likely to be completed no matter overtime; 3) the obligation norm about working is positively correlated with general obligation norms; and, 4) external motivtors will enhance lower obligation orientation subjects' working motivation more than higher obligation orientation subjects' the results verified hypothesis 1 and 3, but didn't support hypothesis 2 and 4 these findings will have great implication in administrating.

Characteristic infrared spectroscopic patterns in the protein bands of human breast cancer tissue

Infrared (IR) spectra of normal, hyperplasia, fibroadenoma and carcinoma tissues of human breast obtained from 96 patients have been determined and analyzed statistically. Several spectral differences were detected in the frequency regions of N-H stretching, amide I, II and III bands: (1) the bands in the region 3000-3600cm-1 shifted to lower frequencies for the carcinomatous tissue; (2) the A(3300)/A(3075) absorbance ratio was significantly higher for the fibroadenoma than for the other types of tissues; (3) the frequency of the a-helix amide I band decreased for the malignant tissue, while the corresponding beta -sheet amide I band frequency increased; (4) the A(1657)/A(1635) and A(1553)/A(1540) absorbance ratios were the highest for fibroadenoma and carcinoma tissues; (5) the A(1680)/A(1657) absorbance ratio decreased significantly in the order of normal > hyperplasia > fibroadenoma > carcinoma; (6) the A(1651)/A(1545) absorbance ratio increased slightly for the fibroadenoma and the carcinoma tissues; (7) the bands at 1204 and 1278 cm(-1), assigned to the vibrational modes of the collagen, did not appear in the original spectra as resolved peaks and were distinctly stronger in the deconvoluted spectra of the carcinoma tissue and (8) the A(1657)/A(1204) and A(1657)/A(1278) absorbance ratios, both yielding information on the relative content of collagen, increased in the order of normal < hyperplasia < carcinoma < fibroadenoma. The said differences imply that the information is useful for the diagnosis of breast cancer and malignant breast abnormalities, and may serve as a basis for further studies on conformational changes in tissue proteins during carcinogenesis. (C) 2001 Elsevier Science B.V. All rights reserved.

Distribution patterns of PCDD/Fs in chlorinated chemicals

The purpose of this research was to determine polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) in five chlorinated chemicals (phthalocyanine copper, phthalocyanine green, chloranil-1 and 2, and triclosan), and to compare their 2,3,7,8-tetrachlordibenzo-IpI-dioxin equivalents (TEQ). The distribution patterns of total PCDD/Fs and 2,3,7,8-substituted PCDD/Fs were elucidated in detail. The TEQ values of toxic PCDD/Fs in all chemicals were in the range of 5.03-1379.55 ng I-TEQ/kg. The contribution of OCDD and OCDF in phthalocyanine green was 75% of the total TEQ. For chloranils, the maximum contribution of toxic PCDD/Fs was from 2,3,7,8-substituted HxCDF and 2,3,7,8-substituted HpCDF. The TEQ of HxCDF and HpCDF in chloranil-1 was 90% and in chloranil-2 was 71%. And the toxic contribution increased with the degree of chlorination for PCDFs. (C) 2005 Elsevier Ltd. All rights reserved.