Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification

A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. (c) 2008 Elsevier B.V. All rights reserved.

Modification of Fe/Cu multilayers under 400 keV Xe20+ irradiation

Two kinds of Fe/Cu multilayers with different modulation wavelength were deposited on cleaved Si(100) substrates and then irradiated at room temperature using 400 keV Xe20+ in a wide range of irradiation fluences. As a comparison, thermal annealing at 300-900 degrees C was also carried out in vacuum. Then the samples were analyzed by XRD and the evolution of crystallite structures induced by irradiation was investigated. The obtained XRD patterns showed that, with increase of the irradiation fluence, the peaks of Fe became weaker, the peaks related to Cu-based fcc solid solution and Fe-based bcc solid solution phase became visible and the former became strong gradually. This implied that the intermixing at the Fe/Cu interface induced by ion irradiation resulted in the formation of the new phases which could not be achieved by thermal annealing. The possible intermixing mechanism of Fe/Cu multilayers induced by energetic ion irradiation was briefly discussed.

Experimental study of the high-voltage breakdown and simulations for the RFQ cooler and buncher RFQ1L

The RFQ cooler and buncher RFQ1L is one of the key parts of the being-built super-heavy nuclide research spectrometer. In order to understand the high-voltage breakdown phenomenon, the voltages between electrodes have been measured. In addition, more extensive simulations have been performed for better understanding and optimizing the RFQ1L work points.

CSRe团簇内靶温度闭环控制器设计；Design of Closed Loop Temperature Controller for CSRe Internal Target System

介绍了国家重大科学工程项目——兰州重离子加速器冷却存储环(HIRFL-CSR)的实验环(CSRe)团簇内靶温度闭环控制器的设计。该控制器给气体喷嘴处测温电阻提供长时间稳定度为0.1%的1mA恒定电流,通过12位ADC得到喷嘴温度,并通过混合信号处理器MSP430F149来实现制冷/加热闭环操作。在多种不同实验气体的情况下,该控制器的温度控制精度小于0.5K。目前,该控制器在现场工作良好。

Heat capacity and enthalpy of fusion of pyrimethanil laurate (C24H37N3O2)

Low-temperature heat capacities of pyrimethanil laurate (C24H37N3O2) were precisely measured with an automated adiabatic calorimeter over the temperature range between T = 78 K and T = 340 K. The sample was observed to melt at (321.52 +/- 0.04) K. The molar enthalpy and entropy of fusion as well as the chemical purity of the compound were determined to be (67244 +/- 11) J (.) mol(-1), (209.28 +/- 0.02) J (.) mol(-1) (.) K-1, (0.9943 +/- 0.0004) mass fraction, respectively. The extrapolated melting temperature for the absolutely pure compound obtained from fractional melting experiments was (322.264 +/- 0.006) K. (C) 2004 Elsevier Ltd. All rights reserved.

长白山北坡森林群落交错区优势树种动态(英文)

基于28个20mx90m样地的调查数据,利用Lotka-Volterra模型,本文分析了长白山北坡阔叶红松(Pinuskoraiensis)林和云冷杉林(也叫暗针叶林)群落交错区优势树种之间的竞争及动态。结果显示:在自然条件下,群落将向两个方向分化,一是以云杉(PiceajezoensisandP.koraiensis)和冷杉(Abiesnephrolepis)为优势的群落,并在达到平衡时冷杉占绝对优势(相对优势度的77.1%):另一种是以红松或云冷杉和阔叶树占绝对优势的针阔混交林,并在达到平衡时,阔叶树在阔叶红松林中占相对优势度的50%,在云冷杉一阔叶林类型中占66%。同时,本研究说明:(1)阔叶红松林和云冷杉林都是长白山气候顶极群落:(2)交错区具有过渡性质:(3)森林群落的分化结果说明演替的方向受局部生境的影响。图1表3参24。

东北阔叶红松林群落类型划分及物种多样性

运用TWINSPAN分类方法,对我国东北阔叶红松林群落类型进行划分,对乔木层树种进行聚类,并对东北地区长白山、大秃顶子山、平顶山和丰林保护区4个样点的物种多样性进行对比研究.结果表明:24个样地中共记录到维管束植物264种,隶属于64科147属.经过聚类,将阔叶红松林划分为3个群落类型组和7个群落类型;同时,将33个乔木树种间的关联性划分为8组.阔叶红松林群落的物种丰富度和多样性为草本层>灌木层>乔木层.在4个样点中,长白山的样地平均物种丰富度最高,为63.长白山和大秃顶子山乔木层和灌木层的物种多样性略高于平顶山和丰林保护区;丰林保护区草本层的物种多样性为2.83,高于其它3个样点.平顶山灌木层和长白山草本层的均匀度最低,分别为0.71和0.80.

黑龙江省东部山区森林植物多样性与环境梯度的相关性研究(英文)

为了探讨森林植物多样性与环境梯度的相关关系,本研究在黑龙江省帽儿山国家森林公园调查了23块不同结构的森林群落,每个群落布设的样方面积为400m2。森林植物多样性的计算采用Shannon-Wiener多样性指数,环境梯度的量化采用Whittaker的集合环境梯度计算方法,同时,采用相似性测度方法计算了Beta多样性指数。结果表明:Shannon-Wiener多样性指数随环境梯度的增大而增大,并且β多样性指数与环境梯度呈线性增加的趋势。图2表3参12。

黑土区春小麦群体物质和能量积累研究

利用大田和实验室测定的方法,测定黑土区春小麦群体根、茎、叶、穗等物质和能量在整个生育期的积累过程,旨在为黑土区农业生态系统研究提供实测依据。结果表明,在整个生育期中,春小麦不同器官鲜物质积累的最大值出现的时期各异;除叶和20 cm以下根外,其它器官干物质累积均随生长发育而增加;各器官含水量差异较大,但都随生长而降低;每个器官能量的累积总量并不都是随生长发育而持续增长,且器官间单位干物质所含能量差异较大,变化范围为0.9×10~4 J·g~(-1)(根,孕穗期)至1.5×10~4 J·g~(-1)(叶,三叶期),根、秸秆、籽粒积累的能量分别为6.8×10~5、78.9×10~5和87.9×10~5 J·m~(-2),分别占总积累能量的3.9％、45.4％、50.7％。同实测法相比,计算法获得的秸秆能值高出31％。因此,测定法较计算法能更精确地反映能量在生态系统中的流动。