蜂猴(Nycticebus coucang)和树鼩(Tupaia belangeri chinensis)mt DNA 10种限制酶物理图谱

本研究用10种限制性内切酶对蜂猴和树鼩肝脏线粒体DNA进行了分析. 蜂猴线粒体DNA, BglⅠHind Ⅲ、PstⅠ、SalⅠ和XhoⅠ均各只1个切点、EcoRⅠ、BglⅡ和Pvu Ⅱ各有2切点, BamHⅠ和 EcoRⅤ 分别有3个切点. 对于树鼩线粒体 DNA, BglⅡ、Hind Ⅲ、PstⅠ、SalⅠ和 XhoⅠ均只1个切点, BamHⅠBglⅠ和 PvuⅡ 分别有2个切点, EcoRⅠ 有3个切点, EcoRⅤ有4个切点. 根据单酶和双酶解片段的数目和分子量, 建立了蜂猴和树鼩线粒体 DNA 的物理图谱。

Genome 10K: A Proposal to Obtain Whole-Genome Sequence for 10 000 Vertebrate Species

The human genome project has been recently complemented by whole-genome assessment sequence of 32 mammals and 24 nonmammalian vertebrate species suitable for comparative genomic analyses. Here we anticipate a precipitous drop in costs and increase in sequ

The Anti-HIV Actions of 7-and 10-Substituted Camptothecins

Camptothecin (CPT), a traditional anti-tumor drug, has been shown to possess anti-HIV-1 activity. To increase the antiviral potency, the anti-HIV activities of two CPT derivatives, 10-hydroxy-CPT and 7-hydroxymethyl-CPT, were evaluated in vitro. The therapy index (TI) of CPT, 10-hydroxy-CPT and 7-hydroxymethyl-CPT against HIV-1(IIIB) in C8166 were 24.2, 4.2 and 198.1, and against clinical isolated strain HIV-1(KM018) in PBMC were 10.3, 3.5 and 66.0, respectively. While the TI of CPT, 10-hydroxy-CPT and 7-hydroxymethyl-CPT against HIV-2(CBL-20) were 34.5, 10.7 and 317.0, respectively, and the TI of the three compounds against HIV-2(ROD) showed the similar values. However, when the antiviral mechanisms were considered, we found there was no inhibition of 7-hydroxymethyl-CPT on viral cell-to-cell transmission, and was no inhibition on reverse transcriptase, protease or integrase in cell-free systems. 7-Hydroxymethyl-CPT showed no selective killing of chronically infected cells after 3 days of incubation. In conclusion, 7-hydroxymethyl-CPT showed more potent anti-HIV activity, while 10-hydroxy-CPT had less efficient activity, compared with the parent CPT. Though the antiviral mechanisms remain to be further elucidated; the modification of -OH residues at C-7 of CPT could enhance the antiviral activity, while of -OH residues at C-10 of CPT had decreased the antiviral activity, which provides the preliminary modification strategy for anti-viral activities enhancement of this compound.

Anti-HIV Actions of 7- and 10-Substituted Camptothecins (vol 15, pg 138, 2010)

The authors thank the anonymous reviewer for helpful comments on the early version of the manuscript. This work was financially supported by the earmarked fund for Modern Agro-industry Technology Research System, the Science Fund for Young Scholars in Sichuan Province (Grant No: ZQ 026-017), and the National 863 Project of China (No. 2008AA101001).

鸟类核型研 Ⅶ: 雉冬10种鸟类

报道了雉科10种鸟的核型, 并依据核型和DNA比较数据提出了对雉科中亚科分类的新观点。 图版1表3参11