218 resultados para CA2 STORES
Resumo:
The vertical distribution of the variables relevant to P forms in sediments were studied in a shallow Chinese freshwater lake (Lake Donghu) in 1997, 1998, 1999 and 2000, to assess the contribution of enzyme to P availability in sediment cores. Sediment P was fractionationd into iron-bound P, calcium-bound P, acid soluble organic P (ASOP) and hot NaOH extractable residual organic P. The former two species made the largest contribution to the sediment P pool. All P species exhibited significantly higher concentrations in different depths at Station I, compared with those found at Station II, except for ASOP. Coupled with these lower ASOP concentrations, the V-max data of alkaline phosphatase, measured on the same samples, were significantly higher at station I. Taken together, ASOP were probably important in supplying the enzymatic substrate (Phosphatase Hydrolyzable Phosphorus, PHP) into interstitial water. Dissolved orthophosphate and PHP concentrations were highly heterogeneous , but peaked in subsurface, paralleled by higher V-max and lower K-m values of alkaline phosphatase, throughout the sediment core. Sediment in the eutrophic lake is not only enriched in available P (iron-bound P), or stores residual P, but also tends to release PHP, thereby inducing the production of alkaline phosphatase and releasing o-P into water column by enzymatic hydrolysis. The latter process may also occur in relatively deep sediment layers.
Resumo:
Silver crucian carp (Carassius auratus gibelio) is a unique triploid bisexual species that can reproduce by gynogenesis. As all other gynogenetic animals, it keeps its chromosome integrity by inhibiting the first meiosis division (no extrusion of the first pole body). To understand the molecular events governing this reproduction mode, suppression subtractive hybridization was used to identify the genes differentially expressed in fully-grown oocytes of the gynogenetic and gonochoristic crucian carp (gyno-carp and gono-carp). From two specific subtractive cDNA libraries, the clones screened out by dot blots and virtual Northern blots were chosen to clone, full-length cDNA by RACE. Four differentially expressed genes were obtained. Two are novel genes and are expressed specifically in the oocytes. The gyno-carp stores much more mRNA of cyclin A2, a new member of the fish A-type cyclin gene, in its fully-grown oocyte than in the gono-carp. The last gene is histone H2A. The histone H2As of these two closely related crucian carps are quite different in the C-terminus. Preliminary characterization of the four genes has been analyzed by nucleotide and deduced amino acid sequence and Northern analysis. (C) 2001 Elsevier Science B.V. All rights reserved.
Resumo:
Cu2+,pHCu2+Cu2+,;pH6Cu2+20 mg/L0.25 mm,,,Na+Ca2+Cu2+,0.1mol/L HCl96.1%Cu2+Thomas,10.94mg/gCu2+,
Resumo:
p21Waf1/Cip1p21 p21 pBV220-p213042p21p21p21 p21suraminBis-ANS10-711suraminp21p21C-CaMp21141-164p21p21141164CaMp21CaM10-7p21CaMHp21141-164CaMCaMCa2+p21141164150151CaM159p21141164CaM14%p21141164CaM
Resumo:
3(cdb3) BL21(DE3)pET28b-cdb337OD6000.61mM IPTG 30cdb3cdb3cdb3, cdb3- cdb3TrpGuHClcdb3 TrppH6.010.0cdb3Tm15pH 7.2pH9.2Cd2+Ca2+Cu2+Co2+Mg2 Zn2+cdb3Trp50Mcdb3cdb3N123TFETFE80% ATPcdb3ATP3mMcdb3
Resumo:
1DNADNADNADNA2DNA-CTABDNADNADNADNA3DNADNADNAAFMDNA pBR322DNADNA4Mg2+Ca2Sr2Ba2+DNADNAMg2Ca2+Sr2+DNABa2+DNADNAB-AB-ASr2-B-A
Resumo:
(rESCs)rESCsrESCs1) rESCs/(NEPs)(RG)(NPs)(GPs)NEPs/RG--NEPs/RGNotchFGFR(GRPs)rESCs2) NO(10M250M SNP)rESCsA2B5+/Nestin/PSA-NCAMNetrin-DCCNOCa26Netrin-DCCNO
Resumo:
AnntoxinAnSFcDNA Anntoxin AnSF cDNA Gene Bank FJ598043Anntoxin 60 Kunitz Anntoxin 3D-NMR Anntoxin 1-62-43-5Kunitz 1-42-3AnSF 123 C Calmadolin EF hESCrNSCAnntoxin AnntoxinAnntoxinrAnntoxin rAnntoxin Anntoxin Anntoxin Kunitz Cone SnailConkunitzin-S1black cobra, Dendroaspis polylepis polylepis-DaTX K 32.8%36.7%fishStonustoxin Anntoxin rat DRGNa+K+Ca2+Anntoxin TTX-SNav K+Ca2+K+Kv1.1Kv1.2Kv1.3Kv2.1 Kv4.2Kv4.3Anntoxin K+K+Anntoxin 3D-NMR NMR PDB ID 2KCR BMRB ID 16094Anntoxin Kunitz RT-PCRWesternBlot ELISA Anntoxin mRNA 29.55.39 4.80 2.02 /Anntoxin Anntoxin Anntoxin Laphygma exigua HubnerEnhydris plumbeaCoturnix coturnixKunming miceLD50 504502500 3000 /Anntoxin AnSF AnSFrAnSF10100 500ng/mlAnSF hESC 10~100 ng/ml hESC 10100 500ng/mlAnSF rNSC 10ng/ml rNSC 500ng/ml AnSF hESC rNSC rNSC RT-PCR AnSF AnSF AnSF AnSF
Resumo:
Tabancus yao Macquart ADP A2(PLA2) HepG2 mRNA1106 cDNA4002344 544205 222attactins mucin ,Hybomitra bimaculata47-82% 55 6 kDa3 Kunitz TYTI Anemonia sulcata AsKC3 SA5II 66% 2.58610-4M 7 kDa65 3 Taymin 43% : 16080140 120g/mL , TYML1 ABO AB , 8 Ca2+ 26 kDa,234 10 Macquaritin-2, (25%-33%),: StejnulxinTMVAADPU46619 :, 24-30 kDa Macquaritin-3 Macquaritin-1 N 16 V N Y C R L P C R G C D Y H V V A V D Y L G L P G R G Y H VPCR, Macquaritin-3 232 23 Macquaritin-1 N V A V D Y L G L P 5 cDNA 5 ,,33.3%-93.0%, 50% 5 5
Resumo:
HAH2 Olsen-POlsen-P1020Ca2-PCa8-PAl-P10HAH2Olsen-P40.9%33.1%23.2%20HAH232.4%24.9%16.7%30Al-PCa2-POlsen-PAl-PCa2-POlsen-P ---47.1%~50.2%H2450kg/hm29.32%15kg/ hm2HA150kg/ hm25.99%4.99%
Resumo:
3090 CuPbZnCdHakansonCdCdCd Cd24hCdCdCd21.714.818429Cd21.7CdCd CdCl-Ca2+ > Mg2+> HCO3-SO42-Na+ Cd
Resumo:
1758-3600m 6175826203200350035873600m22540185225 1.2251028452251010928 1604511745B1394873U/ml16SrDNAB1394Bacillus subtilis60pH 8.0 405060 Mn2+ Mg2+ Ca2+Hg2+ Fe3+ Cu2+ Zn2+ Fe2+PMSF 2.10040 Rhizoctonia solaniCandida albicans373537%35%1845%SHA6Fusarium oxysporum10SHA6, SHA6Aurantimonas altamirensis 3.205SHA4100g/ml83%,400ppm48h38%SHA4Nocardiopsis sp
Resumo:
(1990)8(CK)(M)(N)+(N+M)+(NP)++(NP+M)++(NPK)+++(NPK+M) 19902004(0~20cm)199520012007(0~20cm)18(Ca2-PCa8-PAl-PFe-P)PO-PCa10-PP P(Olsen-P)Y=15.127+2.158x1+0.152x2+0.265x3+0.168x4-0.128x5-0.247x6 (R2=0.9997F=571.78Pr=0.032Yx1x2x3x4x5x6Olsen-PCa2-PCa8-PAl-PFe-PO-PCa10-P) (M)()Ca2-PCa8-PAl-PCa2-PCa8-P(M)Ca2-PCa8-P(M)Ca2-PCa8-P(M)Al-PFe-PO-PCa2-PCa8-P ()PP 8(M)PP(N+M)P
Resumo:
To investigate the roles of intercellular gap junctions and extracellular ATP diffusion in bone cell calcium signaling propagation in bone tissue, in vitro bone cell networks were constructed by using microcontact printing and self-assembled monolayer technologies. In the network, neighboring cells were interconnected through functional gap junctions. A single cell at the center of the network was mechanically stimulated by using an AFM nanoindenter. Intracellular calcium ([Ca2+](i)) responses of the bone cell network were recorded and analyzed. In the untreated groups, calcium propagation from the stimulated cell to neighboring cells was observed in 40% of the tests. No significant difference was observed in this percentage when the intercellular gap junctions were blocked. This number, however, decreased to 10% in the extracellular ATP-pathway-blocked group. When both the gap junction and ATP pathways were blocked, intercellular calcium waves were abolished. When the intracellular calcium store in ER was depleted, the indented cell can generate calcium transients, but no [Ca2+](i) signal can be propagated to the neighboring cells. No [Ca2+](i) response was detected in the cell network when the extracellular calcium source was removed. These findings identified the biochemical pathways involved in the calcium signaling propagation in bone cell networks. Published by Elsevier Ltd.
Resumo:
ArthrobacterK110 8 , ,K110 8 5 5 ,pH 7 0 ,Co2 + Fe2 + ,Ca2 + K110 8 , 5 ,5 5 K110 8 , ,N
