Monitoring microbial populations of sulfate-reducing bacteria using an impedimetric immunosensor based on agglutination assay

An impedimetric immunosensor was fabricated for rapid and non-labeled detection of sulfate-reducing bacteria, Desulforibrio caledoiensis (SRB) by immobilizing lectin-Concanavalin A using an agglutination assay. The immobilization of lectin was conducted using amine coupling on the surface of a gold (Au) electrode assembled with 11-Mercaptounclecanoic acid. Electrochemical impedance spectroscopy (EIS) was used to verify the stepwise assembly of the sensor system. The work conditions of the impedimetric immunosensor, such as pH of the buffer solutions and the incubation time of lectin, were optimized. Faradic impedance spectra for charge transfer for the redox probe Fe(CN)(6)(3-/4-) were measured to determine SRB concentrations. The diameter of the Nyquist diagram that is equal to the charge-transfer resistance (RI) increased with increasing SRB concentration. A linear relationship between R-ct and SRB concentration was obtained in SRB concentration range of 1.8 to 1.8 x 10(7) cfu/ml. The variation of the SRB population during the growth process was also monitored using the impedimetric immunosensor. This approach has great potential for simple, low-cost. and time-saving monitoring of microbial populations. (C) 2009 Elsevier B.V. All rights reserved.

青海东部4种作物群落蒸散规律研究

采用改进的离体连续快速称重法对7~9月燕麦(Avena Sativa)、豌豆(Pisum Sativum)、无芒雀麦(Bromus Innermis)、紫花苜蓿(Medicago Sativa)枝和叶片的蒸腾速率、蒸腾量及土面蒸发量进行了测定。结果表明,豌豆的枝蒸腾速率和叶片蒸腾速率均最高,其次为紫花苜蓿和燕麦,无芒雀麦最低。在7~9月中,8月4种作物整株和叶片的平均日蒸腾量均最高,9月次之,7月最低。4种作物群落土面蒸发量的日变化无明显规律性。