眼镜蛇毒神经生长因子对成年猫坐骨神经损伤后的作用

目的　探讨从眼镜蛇毒分离纯化出的神经生长因子(nerve grow th facto r, N GF) 对成年猫坐骨神经 损伤后的影响。方法　制成成年猫坐骨神经损伤模型, 损伤局部注射蛇毒N GF 2 Lgö(kg·d) , 分别治疗10 d 和30 d, 并与对照组(损伤坐骨神经, 不给药物) 比较。结果　术后10 d, 对照组术侧远端神经纤维数量比治疗组明显减少 (P < 0. 01) , 治疗组术侧足底刺激出现早, 肢体活动恢复快。术后30 d, 治疗组术侧远端神经纤维大量再生, 再生神 经纤维数量已明显超过对照组和术后10 d 组水平(P < 0. 01) , 但结构紊乱, 轴突和郎氏结消失。术侧肢体在连续注 射N GF 16 d 左右出现足底刺激反应消失, 肢体瘫痪等改变。结论　眼镜蛇毒N GF 在神经损伤早期应用能减轻神 经纤维发生的溃变, 促进受损神经的再生与功能恢复; 而损伤局部长时间注射蛇毒N GF 则会导致神经纤维增生过 度, 丧失传导功能。

嗜盐菌中的类视紫红质蛋白

在嗜盐菌中发现了4种含视黄醛的膜蛋白: 细菌视紫红质、氯视紫红质、感觉视紫红质-I(M 慢视紫红质)和感觉视紫红质-Ⅱ(憎光视紫红质)。文中着重介绍了上述4中膜蛋白的结构、 功能及其相似性等方面的研究进展。图6参20

麝属(Moschus)的分类

根据各国博物馆中保存的标本, 用多变量分析的方法, 对古北区特有的麝属进行了分析、比较和进一步整理, 提供了麝属种和亚种检索表。图4参19

不同药物对心理应激猕猴的抗应激作用

以心理应激猕猴为动物模型,比较研究了狒猴应激前后外周血中ACTH、皮质醇、胰岛素以及游离T_(3),T_(4)的变化,并对安定、心得安等药物的抗应激效应进行了初步的探讨。实验结果表明,在应激前后,ACTH、皮质醇呈显著性差异(P<0.05和P<0.01),胰岛素呈上升趋势。安定可使应激动物的ACTH、皮质醇的水平明显降低(P<0.05),但是,对FT_(3),FT_(4)的影响不明显。心得安对各种激素无明显影响。

In vitro anti-HIV and -HSV activity and safety of sodium rutin sulfate as a microbicide candidate

Sodium rutin sulfate (SRS) is a sulfated rutin modified from the natural flavonol glycoside rutin. Here, we investigated its in vitro anti-HIV and -HSV activities and its cytotoxic profile. Fifty percent inhibitory concentration (IC50) values of SRS against HIV-1 X4 virus IIIB, HIV-1 R5 isolates Ada-M and Ba-L were 2.3 +/- 0.2, 4.5 +/- 2.0 and 8.5 +/- 3.8 mu M with a selectivity index (SI) of 563, 575 and 329, respectively. Its IC50 against primary R5 HIV-1 isolate from Yunnan province in China was 13.1 +/- 5.5 mu M, with a Sl of 197. In contrast, unsulfated rutin had no activity against any of the HIV-1 isolates tested. Further study indicated that SRS blocked viral entry and virus-cell fusion likely through interacting with the HIV- I envelope glycoprotein. SRS also demonstrated some activity against human herpes simplex virus (HSV) with an IC50 of 88.3 +/- 0.1 mu M and a Sl of 30. The 50% cytotoxicity concentration (CC50) of SRS was >3.0 mM, as determined in human genital ME 180, HeLa and primary human foreskin fibroblast cells. Minimum inhibitory concentration of SRS for vaginal lactobacilli was >3.0 mM. These results collectively indicate that SRS represents a novel candidate for anti-HIV-1/HSV microbicide development. (C) 2007 Elsevier B.V. All rights reserved.