蛙虹彩病毒cop基因的克隆表达及亚细胞定位

从蛙虹彩病毒(Rana gryliovirus,RGV)基因组中克隆了含凋亡相关结构域的新基因-cop(Caspaserecruit ment domain only protein,COP)基因的全部编码区,成功构建了重组表达载体,进行了原核表达,并在鲤鱼上皮瘤细胞(Epithelioma papulosumcyprini,EPC)中进行了亚细胞定位.序列分析表明,RGVcop基因全长288 bp,编码一个长为95 aa,分子量为10.4×103的推定蛋白.二级结构预测表明其含有5个α螺旋.同源性比对分

控制沙尘暴的植被快速建设技术途径研究——以库布齐沙漠东缘为例

本文根据恢复生态学和景观生态学的基本原理,遵循自然规律和经济规律,依据当地生态条件和地表流沙分布状况,从水土资源优化配置角度出发,结合沙地植物自然分布的趋水性特征,采取以封育措施为主,因地制宜适度造林种草育藻,构建了一种创新的乔灌草藻四为一体的综合快速复合治沙模式,初步实现了脆弱生态系统趋于稳定,生物多样性明显增加,沙尘暴危害被有效遏制的目标。

长期继代柑橘愈伤胚状体诱导后的扫描和透射电镜观察

长期继代的伏令夏橙愈伤组织经扫描电镜和透射电镜观察显示,在MT基本培养基中培养20 d后,细胞之间连接紧密,有少量纤丝相连.贮藏物质和细胞器丰富,细胞表面形成皱褶;50 d后,细胞表面仍有山脉状皱褶.但皱褶幅度减少.细胞团表面的纤丝消失,细胞内游离核糖体增加.淀粉粒的体积减小。在胚状体诱导培养基中培养20 d后,出现疣突状和纤丝结构,有的纤丝形成网络状,细胞质形成一团,没有明显的细胞器的分化,但内质网发达,游离核糖体丰富,未发现淀粉粒和胞间连丝;50 d后,细胞表面的纤丝消失,细胞体积增大,彼此之问的间隙

黑尾近红鲌人工繁殖研究

报道了2001～2003年对长江上游特有鱼类黑尾近红鲌开展人工繁殖试验的结果。黑尾近红亲鱼来源于四川泸州长江上游一级支流龙溪河。催产剂为促黄体素释放激素类似物(LRH A),绒毛膜促性腺激素(HCG)与鲤鱼脑垂体(PG)。采用一次注射法,并用干法进行人工授精。共进行了23次人工催产,催产成功的占16次,有效的占3次,失败的占4次;共注射雌鱼70尾,其中46尾雌鱼排卵,平均催产率65.71%,受精率8.33%～100.0%,孵化率0.50%～94.71%,获得仔鱼29.3万尾。对亲鱼选择、催产方法、孵化与

野生和人工养殖的暗纹东方毒性的比较

国家自然科学基金赤潮重大项目 ( 39790 1 1 0 )

无机盐对蓝藻细胞结构的影响和原生质球形成

国家自然科学基金

用高效液相色谱法测定鱼样中的维生素D_3和E

用高效液相色谱法测定鱼样中的维生素Ｄ＿３和Ｅ徐立红，陈专，徐盈，张甬元（中国科学院水生生物研究所，武汉４３００７２）ＭＥＡＳＵＲＥＭＥＮＴＯＦＶＩＴＡＭＩＮＤ＿３ＡＮＤＶＩＴＡＭＩＮＥＯＦＦＩＳＨＳＡＭＰＬＥＳＢＹＨＰＬＣ￥ＸｕＬｉｈｏｎｇ，Ｃｈｅｎ...

湖北绿球藻目新植物

本文报道采自湖北省绿球藻目的5个新种,即粗角四棘藻、六锥四角藻、钝尖月形藻、湖北胸甲藻和三角空心藻。

鲤痘疮病病原的电镜观察初报

<正> 痘疮病是鲤越冬前后常见的疾病。过去由于该病在池塘中流行不广,危害也不大,因而未引起足够的重视。近年来由于网箱养鱼等高密度养殖技术的推广,该病日益常见而且严重。本文报道了患痘疮病鲤鱼组织的电镜观察。

真(鱼岁)(Phoxinus phoxinus(L.))的能量收支各组分与摄食量、体重及温度的关系

在不同的摄食水平(饥饿—最大量)及温度(5—15℃)下,对1—5g的真(鱼岁)的摄食量、排粪量、排泄量、代谢量,生长量及生化组成作了测定。真(鱼岁)的最大摄食量随体重及温度增加而增加。食物能量平均有6.5％损失于粪便中,5.1％损失于排泄物中。摄食代谢随摄食量增加而增加。在同一温度下,特定生长率与摄食量的关系是一减速增长曲线。当摄食不受限制时,生长率随温度增加而增加;当摄食受限制时,生长率随温度增加而下降。鱼体的干物质含量及能量含量随摄食量增加而增加。

长江三峡地区的原生动物区系研究

作者于1984、1987和1988年对长江三峡地区的原生动物区系组成和生态分布进行了调查。从奉节下至宜昌的峡区共鉴定了320种原生动物,包括鞭毛虫(无色植鞭虫和动鞭毛虫)40种、肉足虫87种和纤毛虫193种。其中有2新种、4新亚种和87种新记录。峡区原生动物绝大部分可在我国其它地区存在,无地理分布差异。根据出现率的高低分析,主要优势种是珍珠映毛虫、钩刺斜管虫、瞬目膜袋虫和小轮毛虫,以第一优势种珍珠映毛虫为代表的成分反映了亚热带特征,代表了东洋界的地理成分。宜昌干流及支流的种类最多,后者为其它江段支流的2.

东湖生态系统污染状况的FUZZY聚类分析

以水色、透明度、五日生化需氧量、总氮、总磷、悬浮物等感官的和生物学的6个参数为依据,用fuzzy聚类分析方法对武汉东湖生态系统的污染状况进行了分析研究,所获得的结果与实际情况相符。同时也简单地介绍和讨论了用于生态学的fuzzy聚类分析的一般方法。

Spatial analysis for spring bloom and nutrient limitation in Xiangxi bay of three Gorges Reservoir

The spatial and temporal dynamics of physical variables, inorganic nutrients and phytoplankton chlorophyll a were investigated in Xiangxi Bay from 23 Feb. to 28 Apr. every six days, including one daily sampling site and one bidaily sampling site. The concentrations of nutrient variables showed ranges of 0.02-3.20 mg/L for dissolved silicate (Si); 0.06-2.40 mg/L for DIN (NH4N + NO2N + NO3N); 0.03-0.56 mg/L for PO4P and 0.22-193.37 mu g/L for chlorophyll a, respectively. The concentration of chlorophyll a and inorganic nutrients were interpolated using GIS techniques. The results indicated that the spring bloom was occurred twice in space during the whole monitoring period (The first one: 26 Feb.-23 Mar.; the second one: 23 Mar.-28 Apr.). The concentration of DIN was always high in the mouth of Xiangxi Bay, and PO4P was high in the upstream of Xiangxi Bay during the whole bloom period. Si seems no obvious difference in space in the beginning of the spring bloom, but showed high heterogeneity in space and time with the development of spring bloom. By comparing the interpolated maps of chlorophyll a and inorganic variables, obvious consumptions of Si and DIN were found when the bloom status was serious. However, no obvious depletion of PO4P was found. Spatial regression analysis could explained most variation of Chl-a except at the begin of the first and second bloom. The result indicated that Si was the factor limiting Chl-a in space before achieved the max area of hypertrophic in the first and second bloom period. When Si was obviously exhausted, DIN became the factor limiting the Chl-a in space. Daily and bidaily monitoring of Site A and B, representing for high DIN: PO4P ratio and low DIN:PO4P ratio, indicated that the concentration of Si was decreased with times at both site A and B, and the dramatically drop of DIN was found in the end monitoring at site B. Multiple stepwise regression analysis indicated that Si was the most important factor affect the development of spring bloom both at site A and B in time series.

Photodegradation of 2-naphthol in aqueous solutions in the presence of humic acid and ferric ions

In this work, the photodegradation of the carcinogenic pollutant 2-naphthol in aqueous solution containing Aldrich humic acid (HA) and ferric ions (Fe(III)) under 125 W and 250 W high pressure mercury lamp (HPML, lambda >= 365 nm) irradiation was investigated. The photooxidation efficiencies were dependent on the pH values, light intensities and Fe(III)/HA concentration in the water, with higher efficiency at pHs 3-4, and 50 mu mol l(-1) Fe(III) with 20 mg l(-1) HA under 250 W HPML. The initial rate of photooxidation increases with increasing, the initial concentration of 2-naphthol from 10 mu mol l(-1) to 100 mu mol l(-1), while do not change at 50 and 100 mu mol l(-1). However, higher removal efficiency of 2-naphthol is achieved at its lower initial concentration of 10 mu mol l(-1), and initial rate of photooxidation is 0.193 mu mol l(-1) min(-1). Dissolved oxygen (DO) plays an important role in the system containing Fe(III)-HA complexes in which Fenton and photo-Fenton reactions were enhanced in the environment. Hydroxyl radicals produced in HA solution with or without ferric ions were determined by using benzene as free radical scavenger and phenol as scavenging products proportional to hydroxyl radicals. By using UV-Vis and excited fluorescence spectrum techniques, the main photooxidation products, which have higher absorption in the region of 240-340 nm, were found, and the mechanisms for the oxidative degradation is proposed.