Molecular orientation of copper phthalocyanine in thin films and their gas-sensing properties

Copper phthalocyanine derivative Langmuir-Blodgett (LB) films were prepared by vertical dipping and horizontal lifting methods. Molecular orientation of copper phthalocyanine derivative in thin films was studied by polarized UV-Vis spectra. The relationship between the molecular orientation of copper phthalocyanine in LB films and their gas-sensing properties was investigated.

Ion-channel sensing of ferricyanide anion based on a supported bilayer lipid membrane

Ferricyanide anion has usually been used as a marker of ion-channel sensors. In this work we first found that ferricyanide, itself, can act as a stimulus to regulate the permeability of sBLM prepared from didodecyldimethylammonium bromide (a kind of synthetic lipid) on a GC electrode. We used cyclic voltammetry and a.c. impedance to investigate this phenomenon. The interaction between sBLM and ferricyanide concerns time. Furthermore, we developed a sensor for ferricyanide anion. The ion-channel sensor is highly sensitive. It can detect ferricyanide concentration as low as 5 muM.

Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing

Edwardsiella tarda is a gram-negative pathogen with a broad host range that includes humans, animals, and fish. Recent studies have shown that the LuxS/autoinducer type 2 (AI-2) quorum sensing system is involved in the virulence of E. tarda. In the present study, it was found that the E. tarda LuxS mutants bearing deletions of the catalytic site (C site) and the tyrosine kinase phosphorylation site, respectively, are functionally inactive and that these dysfunctional mutants can interfere with the activity of the wild-type LuxS. Two small peptides, 5411 and 5906, which share sequence identities with the C site of LuxS, were identified. 5411 and 5906 proved to be inhibitors of AI-2 activity and could vitiate the infectivity of the pathogenic E. tarda strain TX1. The inhibitory effect of 5411 and 5906 on AI-2 activity is exerted on LuxS, with which these peptides specifically interact. The expression of 5411 and 5906 in TX1 has multiple effects (altering biofilm production and the expression of certain virulence-associated genes), which are similar to those caused by interruption of luxS expression. Further study found that it is very likely that 5411 and 5906 can be released from the strains expressing them and, should TX1 be in the vicinity, captured by TX1. Based on this observation, a constitutive 5411 producer (Pseudomonas sp. strain FP3/pT5411) was constructed in the form of a fish commensal isolate that expresses 5411 from a plasmid source. The presence of FP3/pT5411 in fish attenuates the virulence of TX1. Finally, it was demonstrated that fish expressing 5411 directly from tissues exhibit enhanced resistance against TX1 infection.

Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing

Edwardsiella tarda is a gram-negative pathogen with a broad host range that includes humans, animals, and fish. Recent studies have shown that the LuxS/autoinducer type 2 (AI-2) quorum sensing system is involved in the virulence of E. tarda. In the present study, it was found that the E. tarda LuxS mutants bearing deletions of the catalytic site (C site) and the tyrosine kinase phosphorylation site, respectively, are functionally inactive and that these dysfunctional mutants can interfere with the activity of the wild-type LuxS. Two small peptides, 5411 and 5906, which share sequence identities with the C site of LuxS, were identified. 5411 and 5906 proved to be inhibitors of AI-2 activity and could vitiate the infectivity of the pathogenic E. tarda strain TX1. The inhibitory effect of 5411 and 5906 on AI-2 activity is exerted on LuxS, with which these peptides specifically interact. The expression of 5411 and 5906 in TX1 has multiple effects (altering biofilm production and the expression of certain virulence-associated genes), which are similar to those caused by interruption of luxS expression. Further study found that it is very likely that 5411 and 5906 can be released from the strains expressing them and, should TX1 be in the vicinity, captured by TX1. Based on this observation, a constitutive 5411 producer (Pseudomonas sp. strain FP3/pT5411) was constructed in the form of a fish commensal isolate that expresses 5411 from a plasmid source. The presence of FP3/pT5411 in fish attenuates the virulence of TX1. Finally, it was demonstrated that fish expressing 5411 directly from tissues exhibit enhanced resistance against TX1 infection.

SATELLITE REMOTE-SENSING PREDICTION OF JAPANESE PILCHARD FISHING GROUND IN THE HUANGHAI SEA AND THE EAST-CHINA-SEA

The thermophily, fishing season and central fishing ground of Japanese pilchard (Sardinops melanosticta) were studied by using satellite remote sensing (SRS) and other methods in Haizhou Bay and Tsushima waters during 1986-1990. A rapid prediction method of fishing ground is presented. Moreover, the results indicated that the thermophilic values of the fish stock are 11-20 degrees C and both fishing grounds are in increasing temperature process from the beginning to the end of the fishing period. The Japanese pilchards gather vigorously at the sea surface temperature of 15-17 degrees C. The water temperature is a key factor affecting the fishing season and the catch of the fishing ground. The increasing temperature process restricts the fishing season development and central fishing ground formation. The accuracy of 15 predictions made in the Haizhou Bay fishing ground is up to 91.3%, and 37 predictions made in the Tsushima, fishing ground shorten the fish detection time by 13.4% - 22% on the average.

Remote sensing of ocean waves by along-track interferometric synthetic aperture radar

A new wave retrieval method for the Along-Track Interferometric Synthetic Aperture Radar (AT-InSAR) phase image is presented. The new algorithm, named parametric retrieval algorithm (PRA), uses the full nonlinear mapping relations. It differs from previous retrieval algorithms in that it does not require a priori information about the sea state or the wind vector from scatterometer data. Instead, it combines the observed AT-InSAR phase spectrum and assumed wind vector to estimate the wind sea spectrum. The method has been validated using several C-band and X-band HH-polarized AT-InSAR observations collocated with spectral buoy measurements. In this paper, X-band and C-band HH-polarized AT-InSAR phase images of ocean waves are first used to study AT-InSAR wave imaging fidelity. The resulting phase spectra are quantitatively compared with forward-mapped in situ directional wave spectra collocated with the AT-InSAR observations. Subsequently, we combine the parametric retrieval algorithm (PRA) with X-band and C-band HH-polarized AT-InSAR phase images to retrieve ocean wave spectra. The results show that the ocean wavelengths, wave directions, and significant wave heights estimated from the retrieved ocean wave spectra are in agreement with the buoy measurements.

Application of effective medium approximation theory to ocean remote sensing under wave breaking

Based on the effective medium approximation theory of composites, the empirical model proposed by Pandey and Kakar is remedied to investigate the microwave emissivity of sea surface under wave breaking driven by strong wind. In the improved model, the effects of seawater bubbles, droplets and difference in temperature of air and sea interface (DTAS) on the emissivity of sea surface covered by whitecaps are discussed. The model results indicate that the effective emissivity of sea surface increases with DTAS increasing, and the impacts of bubble structures and thickness of whitecaps layer on the emissivity are included in the model by introducing the effective dielectric constant of whitecaps layer. Moreover, a good agreement is obtained by comparing the model results with the Rose's experimental data.

Remote sensing of ocean waves by polarimetric SAR

A new method to measure ocean wave slope spectra using fully polarimetric synthetic aperture radar (POLSAR) data was developed without the need for a complex hydrodynamic modulation transform function. There is no explicit use of a hydrodynamic modulation transfer function. This function is not clearly known and is based on hydrodynamic assumptions. The method is different from those developed by Schuler and colleagues or Pottier but complements their methods. The results estimated from NASA Jet Propulsion Laboratory (JPL) Airborne Synthetic Aperture Radar (AIRSAR) C-band polarimetric SAR data show that the ocean wavelength, wave direction, and significant wave height are in agreement with buoy measurements. The proposed method can be employed by future satellite missions such as RADARSAT-2.

Colorimetric and ratiometric fluorescence sensing of fluoride: Tuning selectivity in proton transfer

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