A linkage map of common carp (Cyprinus carpio) based on AFLP and microsatellite markers

P>Common carp (Cyprinus carpio) is an important fish for aquaculture, but genomics of this species is still in its infancy. In this study, a linkage map of common carp based on Amplified Fragment Length Polymorphism (AFLP) and microsatellite (SSR) markers has been generated using gynogenetic haploids. Of 926 markers genotyped, 151 (149 AFLPs, two SSRs) were distorted and eliminated from the linkage analyses. A total of 699 AFLP and 20 microsatellite (SSR) markers were assigned to the map, which comprised 64 linkage groups and covered 5506.9 cM Kosambi, with an average interval distance of 7.66 cM Kosambi. The normality tests on interval map distances showed a non-normal marker distribution. Visual inspection of the map distance distribution histogram showed a cluster of interval map distances on the left side of the chart, which suggested the occurrence of AFLP marker clusters. On the other hand, the lack of an obvious cluster on the right side showed that there were a few big gaps which need more markers to bridge. The correlation analysis showed a highly significant relatedness between the length of linkage group and the number of markers, indicating that the AFLP markers in this map were randomly distributed among different linkage groups. This study is helpful for research into the common carp genome and for further studies of genetics and marker-assisted breeding in this species.

The Effects of Roughness on Adhesion Hysteresis

Adhesion hysteresis is defined as the difference between the work needed to separate two surfaces and that originally gained on bringing them together. Adhesion hysteresis is a common phenomenon in most surface/interface interactions. This paper studies the effects of surface roughness on adhesion hysteresis. We assumed that the surface asperity height distribution is Gaussian. Numerical simulations based on Fuller's model showed that adhesion hysteresis depended upon a single dimensionless parameter, the adhesion parameter, which represents the statistical average of a competition between the compressive forces exerted by the higher asperities, which are trying to separate the surfaces, and the adhesion forces of the lower asperities which are trying to hold the surfaces together. (C) Koninklijke Brill NV, Leiden, 2010

Fracture of colloidal single-crystal films fabricated by controlled vertical drying deposition

Controlled vertical drying deposition method was used to make high-quality single crystal close-packed colloidal films formed of different radii polystyrene latex spheres on glass substrates coming from a low concentration water suspension (0.1% volume fraction). Regardless of the spheres radii the film thickness was about 6.3 microns. However, cracks destroyed the crystalline film structure during the colloidal film growth. The effect of particle radius (85-215 nm range) on film cracking was systematically studied using in situ optical fracture monitoring. Primary parallel cracks run along the vertical growth direction, later followed by secondary branched cracks in-between the primary cracks due to residual water evaporation. Quantitative theoretical relationship between the cracks spacing and particles radius was derived and shows good agreement with experimental observations. Normalized cracks spacing is related to a reciprocal ratio of the dimensionless particle radius.

Pool Boiling on Wire in Microgravity - What Have We Learned

In the past years, steady pool boiling of degassed R113 on thin platinum wires has been studied systematically in our lab, including experiments in long-term microgravity aboard RS-22, in short-term microgravity in the Drop Tower Beijing / NMLC, and in normal gravity on the ground. Slight enhancement of nucleate boiling heat transfer is observed in microgravity, while dramatic changes of bubble behaviors are much evident. The value of CHF in microgravity is lower than that in normal gravity, but it can be predicted well by the Lienhard-Dhir correlation, although the dimensionless radius in the present case is far beyond its initial application range. The scaling of CHF with gravity is thus much different from the traditional viewpoint. Considering the influence of the Marangoni effects, the different characteristics of bubble behaviors in microgravity have been explained. A new bubble departure model has also been proposed, which can predict the whole observation both in microgravity and in normal gravity.

FLOW-PIPE-SEEPAGE COUPLING ANALYSIS OF SPANNING INITIATION OF A PARTIALLY-EMBEDDED PIPELINE

The initiation of pipeline spanning involves the coupling between the flow over the pipeline and the seepage-flow in the soil underneath the pipeline. The pipeline spanning initiation is experimentally observed and discussed in this article. It is qualitatively indicated that the pressure-drop induced soil seepage failure is the predominant cause for pipeline spanning initiation. A flow-pipe-seepage sequential coupling Finite Element Method (FEM) model is proposed to simulate the coupling between the water flow-field and the soil seepage-field. A critical hydraulic gradient is obtained for oblique seepage failure of the sand in the direction tangent to the pipe. Parametric study is performed to investigate the effects of inflow velocity, pipe embedment on the pressure-drop, and the effects of soil internal friction angle and pipe embedment-to-diameter ratio on the critical flow velocity for pipeline spanning initiation. It is indicated that the dimensionless critical flow velocity changes approximately linearly with the soil internal friction angle for the submarine pipeline partially-embedded in a sandy seabed.

Indentation of power law creep solids by self-similar indenters

For creep solids obeying the power law under tension proposed by Tabor, namely sigma = b(epsilon) over dot(m), it has been established through dimensional analysis that for self-similar indenters the load F versus indentation depth h can be expressed as F(t) = bh(2)(t)[(h) over dot(t)/h(t)](m)Pi(alpha) where the dimensionless factor Pi(alpha) depends on material parameters such as m and the indenter geometry. In this article, we show that by generalizing the Tabor power law to the general three dimensional case on the basis of isotropy, this factor can be calculated so that indentation test can be used to determine the material parameters b and m appearing in the original power law. Hence indentation test can replace tension test. This could be a distinct advantage for materials that come in the form of thin films, coatings or otherwise available only in small amounts. To facilitate application values of this constant are given in tabulated form for a range of material parameters. (C) 2010 Elsevier B.V. All rights reserved.

Controlling parameter for wave types of long flexible riser undergoing vortex-induced vibration

Submerged floating tunnel (SFT) is a popular concept of crossing waterways. The failure of the cable may occur due to vortex-induced-vibration (VIV), and the stability of the cable is crucial to the safety of the entire tunnel. Investigation results in recent years show that the vortex-induced vibration of the flexible cables with large aspect ratio reveals some new phenomena, for example, the vortex-induced wave, multi-mode competition, wide band random vibration, which have brought new challenges to the study of vortex-induced vibration of long flexible cables. In this paper, the dimensionless parameter controlling the wave types of dynamic response of slender cables undergoing vortex-induced vibration is investigated by means of dimensional analysis and finite element numerical simulations. Our results indicate that there are three types of response for a slender cable, i.e. standing wave vibration, traveling wave vibration and intermediate state. Based on dimensional analysis the controlling parameter is found to be related to the system damping including fluid damping and structural damping, order number of the locked-in modes and the aspect ratio of cable. Furthermore through numerical simulations and parameter regression, the expression and the critical value of controlling parameter is presented. At last the physical meaning of the parameter is analyzed and discussed.

Di-nuclear systems studied with the velocity filter SHIP

Various nuclear reactions like quasi-fission, fusion-fission or particle and cluster evaporation from excited compound nuclei were studied in heavy-ion reactions at the velocity filter SHIP of GSI. The velocity filter offers the possibility to detect all reaction products under zero degree relative to the beam direction. Together with the measurement of the product velocity distribution this allows for an identification of the underlying reaction mechanism. This article is focussed on reactions of Mg-25 and Ni-64 beams on Pb-206,Pb-207 targets at energies of 5.9 x A MeV and 8.7 x A MeV. Besides evaporation residues from Mg-25 + Pb-206 collisions we found evidence for rotation and quasi-fission of nuclear molecules formed in the entrance channel after the capture stage. The break-up of the systems showed a preferred clustering leading to isotopes in the region 84 <= Z <= 88 and 122 <= N <= 127 of the chart of nuclei.

Surface pigments,algal biomass profiles,and potential production of the euphotic layer:Relationships reinvestigated in view of remote-sensing applications

Maps of surface chlorophyllous pigment (Chl a + Pheo a) are currently produced from ocean color sensors. Transforming such maps into maps of primary production can be reliably done only by using light-production models in conjuction with additional information about the column-integrated pigment content and its vertical distribution. As a preliminary effort in this direction. $\ticksim 4,000$ vertical profiles pigment (Chl a + Pheo a) determined only in oceanic Case 1 waters have been statistically analyzed. They were scaled according to dimensionless depths (actual depth divided by the depth of the euphotic layer, $Z_e$) and expressed as dimensionless concentrations (actual concentration divided by the mean concentration within the euphotic layer). The depth $Z_e$ generally unknown, was computed with a previously develop bio-optical model. Highly sifnificant relationships were found allowing $\langle C \rangle_tot$, the pigment content of the euphotic layer, to be inferred from the surface concentration, $\bar C_pd$, observed within the layer of one penetration depth. According to their $\bar C_pd$ values (ranging from $0.01 to > 10 mg m^-3$), we categorized the profiles into seven trophic situations and computed a mean vertical profile for each. Between a quasi-uniform profile in eutrophic waters and a profile with a strong deep maximum in oligotrophic waters, the shape evolves rather regularly. The wellmixed cold waters, essentially in the Antarctic zone, have been separately examined. On average, their profiles are featureless, without deep maxima, whatever their trophic state. Averaged values their profiles are featureless, without deep maxima, whatever their trophic state. Averaged values their profiles are featureless, without deep maxima, whatever their trophic state. Averaged values of $ρ$, the ratio of Chl a tp (Chl a + Pheo a), have also been obtained for each trophic category. The energy stored by photosynthesizing algae, once normalized with respect to the integrated chlorophyll biomass $\langle C \rangle _tot $ is proportional to the available photosythetic energy at the surface via a parameter $ψ∗$ which is the cross-section for photosynthesis per unit of areal chlorophyll. By tanking advantage of the relative stability of $ψ∗.$ we can compute primary production from ocean color data acquired from space. For such a computation, inputs are the irradiance field at the ocean surface, the "surface" pigment from which $\langle C \rangle _tot$ can be derived, the mean $ρ value pertinent to the trophic situation as depicted by the $\bar C_pd or $\langle C \rangle _tot$ values, and the cross-section $ψ∗$. Instead of a contant $ψ∗.$ value, the mean profiles can be used; they allow the climatological field of the $ψ∗.$ parameter to be adjusted through the parallel use of a spectral light-production model.

基于考生比例的考场编排问题的分治算法

根据高考考场编排的一般要求和约束条件,建立了相应的数学模型,提出了基于考生比例的考场编排问题的分治算法,给出了算法的具体步骤,分析了算法的复杂度,验证了算法的合理性和有效性。实验结果表明,该算法能有效控制考生的分布,编排过程完全可以控制,最大程度地避免了前后左右相邻考生属同一中学,编排结果达到了比较理想的均衡状态。该算法速度快、效率高、易于实现、继承性强,很容易推广到其它类似问题的求解。

Robustness and dissipation of mitogen-activated protein kinases signal transduction network: Underlying funneled landscape against stochastic fluctuations

We uncovered the underlying energy landscape of the mitogen-activated protein kinases signal transduction cellular network by exploring the statistical natures of the Brownian dynamical trajectories. We introduce a dimensionless quantity: The robustness ratio of energy gap versus local roughness to measure the global topography of the underlying landscape. A high robustness ratio implies funneled landscape. The landscape is quite robust against environmental fluctuations and variants of the intrinsic chemical reaction rates.

Application of forecasting structural cracks technique of 3D move in Chengdao buried hill

3DMove software, based on the three-dimension structural model of geologic interpretation, can forecast reservoir cracks from the point of view of formation of the structural geology, and analyze the characteristics of the cracks. 3DMove software dominates in forecasting cracks. We forecast the developments and directions of the cracks in Chengbei buried hill with the application of forecasting technique in 3DMove software, and obtain the chart about strain distributing on top in buried hill and the chart about relative density and orientation and the chart about the analysis of crack unsealing. In Chengbei 30 buried hill zone, north-west and north-east and approximately east-west cracks in Cenozoic are very rich and the main directions in every fault block are different. Forecasting results that are also verified by those of drilling approximately accord with the data from well logging, the case of which shows that the technique has the better ability in forecasting cracks, and takes more effects on exploration and exploitation of crack reservoir beds in ancient buried hill reservoirs.

Determination of fractional energy loss of waves in nearshore waters using an improved high-order Boussinesq-type model

Fractional energy losses of waves due to wave breaking when passing over a submerged bar are studied systematically using a modified numerical code that is based on the high-order Boussinesq-type equations. The model is first tested by the additional experimental data, and the model's capability of simulating the wave transformation over both gentle slope and steep slope is demonstrated. Then, the model's breaking index is replaced and tested. The new breaking index, which is optimized from the several breaking indices, is not sensitive to the spatial grid length and includes the bottom slopes. Numerical tests show that the modified model with the new breaking index is more stable and efficient for the shallow-water wave breaking. Finally, the modified model is used to study the fractional energy losses for the regular waves propagating and breaking over a submerged bar. Our results have revealed that how the nonlinearity and the dispersion of the incident waves as well as the dimensionless bar height (normalized by water depth) dominate the fractional energy losses. It is also found that the bar slope (limited to gentle slopes that less than 1:10) and the dimensionless bar length (normalized by incident wave length) have negligible effects on the fractional energy losses.

Statistical distribution of wave-surface elevation for second-order random directional ocean waves in finite water depth

Based on the second-order random wave solutions of water wave equations in finite water depth, a statistical distribution of the wave-surface elevation is derived by using the characteristic function expansion method. It is found that the distribution, after normalization of the wave-surface elevation, depends only on two parameters. One parameter describes the small mean bias of the surface produced by the second-order wave-wave interactions. Another one is approximately proportional to the skewness of the distribution. Both of these two parameters can be determined by the water depth and the wave-number spectrum of ocean waves. As an illustrative example, we consider a fully developed wind-generated sea and the parameters are calculated for various wind speeds and water depths by using Donelan and Pierson spectrum. It is also found that, for deep water, the dimensionless distribution reduces to the third-order Gram-Charlier series obtained by Longuet-Higgins [J. Fluid Mech. 17 (1963) 459]. The newly proposed distribution is compared with the data of Bitner [Appl. Ocean Res. 2 (1980) 63], Gaussian distribution and the fourth-order Gram-Charlier series, and found our distribution gives a more reasonable fit to the data. (C) 2002 Elsevier Science B.V. All rights reserved.

Applications of improved 2-D numerical wave tanks in wave breaking

Song and Banner (2002, henceforth referred to as SB02) used a numerical wave tank (developed by Drimer and Agnon, and further refined by Segre, henceforth referred to as DAS) to study the wave breaking in the deep water, and proposed a dimensionless breaking threshold that based on the behaviour of the wave energy modulation and focusing during the evolution of the wave group. In this paper, two modified DAS models are used to further test the SB02's results, the first one (referred to MDAS1) corrected many integral calculation errors appeared in the DAS code, and the second one (referred to MDAS2) replaced the linear boundary element approximation of DAS into the cubic element on the free surface. Researches show that the results of MDAS1 are the same with those of DAS for the simulations of deep water wave breaking, but, the different values of the wavemaker amplitude, the breaking time and the maximum local average energy growth rate delta(max) for the marginal breaking cases are founded by MDAS2 and MDAS1. However, MDAS2 still satisfies the SB02' s breaking threshold. Furthermore, MDAS1 is utilized to study the marginal breaking case in the intermediate water depth when wave passes over a submerged slope, where the slope is given by 1 : 500, 1 : 300, 1 : 150 or 1 : 100. It is found that the maximum local energy density U increases significantly if the slope becomes steeper, and the delta(max) decreases weakly and increases intensively for the marginal recurrence case and marginal breaking case respectively. SB02's breaking threshold is still valid for the wave passing over a submerged slope gentler than 1 : 100 in the intermediate water depth.