Preparation of a blocked isocyanate compound and its grafting onto styrene-b-(ethylene-co-1-butene)-b-styrene triblock copolymer

The epsilon-caprolactam was used to block the isocyanate group to enhance the storage stability of allyl (3-isocyanate-4-tolyl) carbamate. The spectra of FTIR and NMR showed that blocked allyl (3-isocyanate-4-tolyl) carbamate (BTAI) possesses two chemical functions, an 1-olefin double bond and a blocked isocyanate group. The FTIR spectrum showed BTAI could regenerate isocyanate group at elevated temperature. DSC and TG/DTA indicated the minimal dissociation temperature was about 135 degrees C and the maximal dissociation rate appeared at 226 degrees C. Then the styrene-b-(ethylene-co-1-butene)-b-styrene triblock copolymer (SEBS) was functionalized by BTAI via melt free radical grafting. The effect of temperature, monomer and initiator concentrations on the grafting degree and grafting efficiency was evaluated. The highest grafting degree was obtained at 200 degrees C. The grafting degree and grafting efficiency increased with the enhanced concentration of BTAI or initiator.

G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?

Some G-quadruplex DNA aptamers have been found to strongly bind hemin to form DNAzymes with peroxidase-like activity. To help determine the most suitable DNAzymes and to understand how they work, five previously reported G-quadruplex aptamers were compared for their binding affinity and then the potential catalytic mechanism of their corresponding hemin-G-quadruplex DNAzymes was explored. Among these aptamers, a G-quadruplex named AGRO100 was shown to possess the highest hemin-binding affinity and the best DNAzyme function. This means that AGRO100 is the most ideal candidate for DNAzyme-based analysis. Furthermore, we found the peroxidase-like activity of DNAzyme to be primarily dependent on the concentration of H2O2 and independent of that of the peroxidase substrate (that is, 2,2-azino-bis(3-ethytbenzothiazoline-6-sulfonic acid)diammonium salt). Accordingly, a reaction mechanism for DNAzyme-catalyzed peroxidation is proposed. This study provides new insights into the G-quadruplex-based DNAzymes and will help us to further extend their applications in the analytical field.

Discovery of genes involved in defense/immunity functions in a haemocytes cDNA library from Fenneropenaeus chinensis by ESTs annotation

Crustacean haemocytes play important roles in the host immune response including recognition, phagocytosis, melanization, cytotoxicity and inter-cellular signal communication. Expressed sequence tags (ESTs) analysis is proved to be an efficient approach not only for gene discovery, but also for gene expression profiles performance. In order to further understand the innate immune system and defense mechanisms of Chinese shrimp at molecular level, complementary DNA library is constructed from the haemocyte tissue of Fenneropenaeus chinensis. A total of 2371 cDNA clones are successfully sequenced and the average sequence length is 460 bp. About 50% are identified as orthologs of known genes from other organisms by BLASTx and BLASTn program. By sequences comparability and analysis, 34 important genes including 177 ESTs are identified that may be involved in defense or immune functions in shrimp based on the known knowledge. These genes are categorized into five categories according to their putative functions in shrimp immune system: 13 genes are different types of antimicrobial peptides (AMP, penaeidin, antilipopolysaccharide factor, etc.), and their proportion is about 3 8%; 11 genes belong to prophenoloxidase system (prophenoloxidase, serine proteinase, serine proteinase inhibitor, etc.), and their proportion is about 32%; five genes have high homology with clotting protein (lectin, transglutaminase, etc), and their proportion is about 15%; three genes may be involved in inter-cell signal communication (peroxinectin, integrin), and their proportion is about 9%; two genes have been identified to be chaperone proteins (Hsc70, thioredoxin peroxidase), and their proportion is about 6%. These EST sequences enrich our understanding of the immune genes of F chinensis and will help farther experimental research into immune factors and improve our knowledge of the immune mechanisms of shrimp. (c) 2007 Elsevier B.V. All rights reserved.

On SAR wind speed ambiguities and related geophysical model functions

Recent investigations show that normalized radar cross sections for C-band microwave sensors decrease under high wind conditions with certain incident angles instead of increase, as is the case for low to moderate wind speeds. This creates the problem of ambiguities in high wind speed retrievals from synthetic aperture radar (SAR). In the present work, four geophysical model functions (GMFs) are studied, namely the high wind C-band model 4 (CMOD4HW), C-band model 5 (CMOD5), the high wind vertical polarized GMF (HWGMF_VV), and the high wind horizontal polarized GMF (HWGMF_HH). Our focus is on model behaviours relative to wind speed ambiguities. We show that, except for CMOD4HW, the other GMFs exhibit the wind speed ambiguity problem. To consider this problem in high wind speed retrievals from SAR, we focus on hurricanes and propose a method to remove the speed ambiguity using the dominant hurricane wind structure.

Forms and functions of inorganic carbon in the Jiaozhou Bay sediments

Inorganic carbon forms and their influencing factors, mutual transformation and contribution to carbon cycling in the Jiaozhou Bay sediments were discussed. The results show that inorganic carbon in sediments could be divided into five forms: NaCl form, NH3 center dot H2O form, NaOH form, NH2OH center dot HCl form and HCl form. Thereinto, NH2OH center dot HCl form and HCl form account for more than 70% of total inorganic carbon. There was close relationship among every form of inorganic carbon and their correlativity was clearly different with different sedimentary environment except the similar strong positive correlation among NH2OH center dot HCl form, HCl form and total inorganic carbon in all regions of the Jiaozhou Bay. All forms of inorganic carbon were influenced by organic carbon, pH, Eh, Es, nitrogen and phosphorus in sediments, but their influence had different characteristics in different regions. Every farm of inorganic carbon transformed into each other continuously during early diagenesis of sediments and the common phenomenon was that NaCl form, NH3 center dot H2O form, NaOH form and NH2OH center dot HCl form might transform into steady HCl form. NaCl form, NH3 center dot H2O form, NaOH form and NH2OH center dot HCl form could participate in carbon recycle and they are potential carbon source; HCl form may be buried for a long time in sediments, and it may be one of the final resting places of atmospheric CO2. Inorganic carbon which entered into sediments was about 4.98 x 10(10) g in the Jiaozhou Bay every year, in which about 1.47x10(10) g of inorganic carbon might be buried for a long time and about 3.51. x 10(10) g of inorganic carbon might return into seawater and take part in carbon recycling.

小波域地震资料的处理方法（WSSP）研究

The modeling formula based on seismic wavelet can well simulate zero - phase wavelet and hybrid-phase wavelet, and approximate maximal - phase and minimal - phase wavelet in a certain sense. The modeling wavelet can be used as wavelet function after suitable modification item added to meet some conditions. On the basis of the modified Morlet wavelet, the derivative wavelet function has been derived. As a basic wavelet, it can be sued for high resolution frequency - division processing and instantaneous feature extraction, in acoordance with the signal expanding characters in time and scale domains by each wavelet structured. Finally, an application example proves the effectiveness and reasonability of the method. Based on the analysis of SVD (Singular Value Decomposition) filter, by taking wavelet as basic wavelet and combining SVD filter and wavelet transform, a new de - noising method, which is Based on multi - dimension and multi-space de - noising method, is proposed. The implementation of this method is discussed the detail. Theoretical analysis and modeling show that the method has strong capacity of de - noising and keeping attributes of effective wave. It is a good tool for de - noising when the S/N ratio is poor. To give prominence to high frequency information of reflection event of important layer and to take account of other frequency information under processing seismic data, it is difficult for deconvolution filter to realize this goal. A filter from Fourier Transform has some problems for realizing the goal. In this paper, a new method is put forward, that is a method of processing seismic data in frequency division from wavelet transform and reconstruction. In ordinary seismic processing methods for resolution improvement, deconvolution operator has poor part characteristics, thus influencing the operator frequency. In wavelet transform, wavelet function has very good part characteristics. Frequency - division data processing in wavelet transform also brings quite good high resolution data, but it needs more time than deconvolution method does. On the basis of frequency - division processing method in wavelet domain, a new technique is put forward, which involves 1) designing filter operators equivalent to deconvolution operator in time and frequency domains in wavelet transform, 2) obtaining derivative wavelet function that is suitable to high - resolution seismic data processing, and 3) processing high resolution seismic data by deconvolution method in time domain. In the method of producing some instantaneous characteristic signals by using Hilbert transform, Hilbert transform is very sensitive to high - frequency random noise. As a result, even though there exist weak high - frequency noises in seismic signals, the obtained instantaneous characteristics of seismic signals may be still submerged by the noises. One method for having instantaneous characteristics of seismic signals in wavelet domain is put forward, which obtains directly the instantaneous characteristics of seismic signals by taking the characteristics of both the real part (real signals, namely seismic signals) and the imaginary part (the Hilbert transfom of real signals) of wavelet transform. The method has the functions of frequency division and noise removal. What is more, the weak wave whose frequency is lower than that of high - frequency random noise is retained in the obtained instantaneous characteristics of seismic signals, and the weak wave may be seen in instantaneous characteristic sections (such as instantaneous frequency, instantaneous phase and instantaneous amplitude). Impedance inversion is one of tools in the description of oil reservoir. one of methods in impedance inversion is Generalized Linear Inversion. This method has higher precision of inversion. But, this method is sensitive to noise of seismic data, so that error results are got. The description of oil reservoir in researching important geological layer, in order to give prominence to geological characteristics of the important layer, not only high frequency impedance to research thin sand layer, but other frequency impedance are needed. It is difficult for some impedance inversion method to realize the goal. Wavelet transform is very good in denoising and processing in frequency division. Therefore, in the paper, a method of impedance inversion is put forward based on wavelet transform, that is impedance inversion in frequency division from wavelet transform and reconstruction. in this paper, based on wavelet transform, methods of time - frequency analysis is given. Fanally, methods above are in application on real oil field - Sansan oil field.